Restriction map for
pFN21AB3680
(Linear) (Six-base) MAPSORT of: KIBB3680.seq Check: 8665 from: 1 to: 2853
KIBB3680.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 488 1532 2214 2726 2853
Size: 488 1044 682 512 127
Fragments arranged by size:
1044 682 512 488 127
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 856 2853
Size: 856 1997
AlwNI CAG_nnn'CTG
Cuts at: 0 154 493 715 1581 2853
Size: 154 339 222 866 1272
Fragments arranged by size:
1272 866 339 222 154
ApoI r'AATT_y
Cuts at: 0 2348 2530 2853
Size: 2348 182 323
Fragments arranged by size:
2348 323 182
AvaI C'yCGr_G
Cuts at: 0 649 2853
Size: 649 2204
BamHI G'GATC_C
Cuts at: 0 1257 2853
Size: 1257 1596
BanII G_rGCy'C
Cuts at: 0 821 1730 2770 2853
Size: 821 909 1040 83
Fragments arranged by size:
1040 909 821 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 1948 2403 2698 2853
Size: 1948 455 295 155
Fragments arranged by size:
1948 455 295 155
BclI T'GATC_A
Cuts at: 0 1304 1503 2853
Size: 1304 199 1350
Fragments arranged by size:
1350 1304 199
BglII A'GATC_T
Cuts at: 0 1401 2853
Size: 1401 1452
BlpI GC'TnA_GC
Cuts at: 0 31 305 2853
Size: 31 274 2548
Fragments arranged by size:
2548 274 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 192 664 736 1724 1870 2853
Size: 192 472 72 988 146 983
Fragments arranged by size:
988 983 472 192 146 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 651 2853
Size: 651 2202
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 692 940 1079 1196 1817 1898 2853
Size: 149 543 248 139 117 621 81 955
Fragments arranged by size:
955 621 543 248 149 139 117 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 211 2853
Size: 211 2642
BsaBI GATnn'nnATC
Cuts at: 0 1505 2597 2853
Size: 1505 1092 256
Fragments arranged by size:
1505 1092 256
BsaHI Gr'CG_yC
Cuts at: 0 2814 2853
Size: 2814 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 248 278 690 720 1871 1901 2853
Size: 248 30 412 30 1151 30 952
Fragments arranged by size:
1151 952 412 248 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 475 2125 2161 2853
Size: 475 1650 36 692
Fragments arranged by size:
1650 692 475 36
BseYI C'CCAG_C
Cuts at: 0 1375 2281 2853
Size: 1375 906 572
Fragments arranged by size:
1375 906 572
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 1625 1754 2482 2853
Size: 701 924 129 728 371
Fragments arranged by size:
924 728 701 371 129
BsiHKAI G_wGCw'C
Cuts at: 0 821 1011 2853
Size: 821 190 1842
Fragments arranged by size:
1842 821 190
BsmI GAATG_Cn'
Cuts at: 0 2568 2853
Size: 2568 285
Bsp1286I G_dGCh'C
Cuts at: 0 821 1011 1730 2770 2853
Size: 821 190 719 1040 83
Fragments arranged by size:
1040 821 719 190 83
BspHI T'CATG_A
Cuts at: 0 92 1148 2853
Size: 92 1056 1705
Fragments arranged by size:
1705 1056 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1761 2853
Size: 1761 1092
BsrBI CCG'CTC
Cuts at: 0 1617 2853
Size: 1617 1236
BsrDI GCAATG_nn'
Cuts at: 0 431 432 1475 2853
Size: 431 1 1043 1378
Fragments arranged by size:
1378 1043 431 1
BsrGI T'GTAC_A
Cuts at: 0 2573 2853
Size: 2573 280
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1006 1900 2853
Size: 1006 894 953
Fragments arranged by size:
1006 953 894
BstEII G'GTnAC_C
Cuts at: 0 536 642 2853
Size: 536 106 2211
Fragments arranged by size:
2211 536 106
BstXI CCAn_nnnn'nTGG
Cuts at: 0 549 1818 2853
Size: 549 1269 1035
Fragments arranged by size:
1269 1035 549
BstYI r'GATC_y
Cuts at: 0 1257 1401 2001 2639 2853
Size: 1257 144 600 638 214
Fragments arranged by size:
1257 638 600 214 144
Bsu36I CC'TnA_GG
Cuts at: 0 2153 2853
Size: 2153 700
BtgI C'CryG_G
Cuts at: 0 52 1241 1280 2853
Size: 52 1189 39 1573
Fragments arranged by size:
1573 1189 52 39
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1931 2853
Size: 1931 922
BtsI GCAGTG_nn'
Cuts at: 0 1296 1538 2248 2615 2853
Size: 1296 242 710 367 238
Fragments arranged by size:
1296 710 367 242 238
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1044 1079 2853
Size: 1044 35 1774
Fragments arranged by size:
1774 1044 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2593 2853
Size: 2593 260
EaeI y'GGCC_r
Cuts at: 0 156 891 2135 2654 2853
Size: 156 735 1244 519 199
Fragments arranged by size:
1244 735 519 199 156
EarI CTCTTCn'nnn_
Cuts at: 0 452 620 1319 1439 2153 2853
Size: 452 168 699 120 714 700
Fragments arranged by size:
714 700 699 452 168 120
EcoICRI GAG'CTC
Cuts at: 0 819 2853
Size: 819 2034
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 149 488 692 940 1079 1196 1532 1817
Size: 149 339 204 248 139 117 336 285
Cuts at: 1817 1898 2214 2726 2853
Size: 81 316 512 127
Fragments arranged by size:
512 339 336 316 285 248 204 149
139 127 117 81
EcoNI CCTnn'n_nnAGG
Cuts at: 0 919 2853
Size: 919 1934
EcoO109I rG'GnC_Cy
Cuts at: 0 933 2853
Size: 933 1920
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 160 192 559 591 1114 1146
Size: 40 32 88 32 367 32 523 32
Cuts at: 1146 1561 1593 2853
Size: 415 32 1260
Fragments arranged by size:
1260 523 415 367 88 40 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1222 2483 2853
Size: 1222 1261 370
Fragments arranged by size:
1261 1222 370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1341 1446 2853
Size: 637 704 105 1407
Fragments arranged by size:
1407 704 637 105
MscI TGG'CCA
Cuts at: 0 893 2656 2853
Size: 893 1763 197
Fragments arranged by size:
1763 893 197
MslI CAynn'nnrTG
Cuts at: 0 547 1147 1816 2399 2608 2853
Size: 547 600 669 583 209 245
Fragments arranged by size:
669 600 583 547 245 209
MspA1I CmG'CkG
Cuts at: 0 1876 2527 2819 2853
Size: 1876 651 292 34
Fragments arranged by size:
1876 651 292 34
NcoI C'CATG_G
Cuts at: 0 52 1241 1280 2853
Size: 52 1189 39 1573
Fragments arranged by size:
1573 1189 52 39
NspI r_CATG'y
Cuts at: 0 1968 2853
Size: 1968 885
PasI CC'CwG_GG
Cuts at: 0 1592 2853
Size: 1592 1261
PflMI CCAn_nnn'nTGG
Cuts at: 0 1981 2853
Size: 1981 872
PfoI T'CCnGG_A
Cuts at: 0 917 2853
Size: 917 1936
PpuMI rG'GwC_Cy
Cuts at: 0 933 2853
Size: 933 1920
PstI C_TGCA'G
Cuts at: 0 1758 1875 2255 2388 2420 2853
Size: 1758 117 380 133 32 433
Fragments arranged by size:
1758 433 380 133 117 32
PvuII CAG'CTG
Cuts at: 0 1876 2527 2853
Size: 1876 651 326
Fragments arranged by size:
1876 651 326
SacI G_AGCT'C
Cuts at: 0 821 2853
Size: 821 2032
SapI GCTCTTCn'nnn_
Cuts at: 0 1439 2853
Size: 1439 1414
ScaI AGT'ACT
Cuts at: 0 202 1869 2853
Size: 202 1667 984
Fragments arranged by size:
1667 984 202
SfcI C'TryA_G
Cuts at: 0 485 563 1118 1754 1871 2251 2313 2384
Size: 485 78 555 636 117 380 62 71
Cuts at: 2384 2416 2853
Size: 32 437
Fragments arranged by size:
636 555 485 437 380 117 78 71
62 32
SmaI CCC'GGG
Cuts at: 0 651 2853
Size: 651 2202
StuI AGG'CCT
Cuts at: 0 2469 2853
Size: 2469 384
StyI C'CwwG_G
Cuts at: 0 52 1241 1280 2853
Size: 52 1189 39 1573
Fragments arranged by size:
1573 1189 52 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 987 2853
Size: 987 1866
TatI w'GTAC_w
Cuts at: 0 200 904 1867 2573 2853
Size: 200 704 963 706 280
Fragments arranged by size:
963 706 704 280 200
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 231 248 263 280 666 690 698 722
Size: 231 17 15 17 386 24 8 24
Cuts at: 722 2853
Size: 2131
Fragments arranged by size:
2131 386 231 24 24 17 17 15
8
Tth111I GACn'n_nGTC
Cuts at: 0 2755 2853
Size: 2755 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1248 2853
Size: 1248 1605
XmaI C'CCGG_G
Cuts at: 0 649 2853
Size: 649 2204
XmnI GAAnn'nnTTC
Cuts at: 0 383 2333 2853
Size: 383 1950 520
Fragments arranged by size:
1950 520 383
Enzymes that do cut:
AcuI AlfI AlwNI ApoI AvaI BamHI BanII BbsI
BclI BglII BlpI BmrI BplI BpmI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII MmeI
MscI MslI MspA1I NcoI NspI PasI PflMI PfoI
PpuMI PstI PvuII SacI SapI ScaI SfcI SmaI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI Bme1580I BmgBI BmtI Bpu10I BpuEI BsaAI
BsaWI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BssSI
BstBI BstZ17I ClaI DraI DraIII EagI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
XbaI XhoI ZraI