(Circular) (Six-base) MAPSORT of: pFN21AB3657.seq Check: 8839 from: 1 to: 5785 pFN21AB3657 5785 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2287 2287 Size: 5785 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3595 278 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2850 3128 1937 Size: 913 278 4594 Fragments arranged by size: 4594 913 278 Acc65I G'GTAC_C Cuts at: 3106 3106 Size: 5785 AclI AA'CG_TT Cuts at: 1563 3912 4285 1563 Size: 2349 373 3063 Fragments arranged by size: 3063 2349 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2516 2684 3848 5232 5500 1632 Size: 884 168 1164 1384 268 1917 Fragments arranged by size: 1917 1384 1164 884 268 168 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5588 AflIII A'CryG_T Cuts at: 1203 2149 4684 5661 1203 Size: 946 2535 977 1327 Fragments arranged by size: 2535 1327 977 946 AgeI A'CCGG_T Cuts at: 4616 4616 Size: 5785 AhdI GACnn_n'nnGTC Cuts at: 4514 4514 Size: 5785 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5785 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5785 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2656 2688 2656 Size: 32 5753 AlwNI CAG_nnn'CTG Cuts at: 2041 2083 3528 5100 2041 Size: 42 1445 1572 2726 Fragments arranged by size: 2726 1572 1445 42 ApaLI G'TGCA_C Cuts at: 3842 4998 3842 Size: 1156 4629 ApoI r'AATT_y Cuts at: 1493 2297 2403 3094 3320 3472 5581 1493 Size: 804 106 691 226 152 2109 1697 Fragments arranged by size: 2109 1697 804 691 226 152 106 AseI AT'TA_AT Cuts at: 160 1032 4339 160 Size: 872 3307 1606 Fragments arranged by size: 3307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 AvaI C'yCGr_G Cuts at: 1870 1943 2066 3110 1870 Size: 73 123 1044 4545 Fragments arranged by size: 4545 1044 123 73 BamHI G'GATC_C Cuts at: 3115 3482 3115 Size: 367 5418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2062 2102 2831 3106 4561 Size: 325 217 627 275 40 729 275 1455 Cuts at: 4561 618 Size: 1842 Fragments arranged by size: 1842 1455 729 627 325 275 275 217 40 BanII G_rGCy'C Cuts at: 1391 2099 2575 3104 1391 Size: 708 476 529 4072 Fragments arranged by size: 4072 708 529 476 BbeI G_GCGC'C Cuts at: 2106 2106 Size: 5785 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2490 928 Size: 971 591 4223 Fragments arranged by size: 4223 971 591 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3976 4010 1903 Size: 34 2039 34 3678 Fragments arranged by size: 3678 2039 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3677 4887 3677 Size: 1210 4575 BclI T'GATC_A Cuts at: 1548 2974 1548 Size: 1426 4359 BfrBI ATG'CAT Cuts at: 2599 2637 2599 Size: 38 5747 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4396 136 Size: 107 122 71 631 605 2724 1525 Fragments arranged by size: 2724 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2338 5780 2338 Size: 3442 2343 BlpI GC'TnA_GC Cuts at: 3202 3202 Size: 5785 Bme1580I G_kGCm'C Cuts at: 1790 2067 3846 5002 1790 Size: 277 1779 1156 2573 Fragments arranged by size: 2573 1779 1156 277 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5785 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3516 4474 442 Size: 416 53 83 399 24 2099 958 1753 Fragments arranged by size: 2099 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5785 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2073 2155 2312 2879 4445 1239 Size: 834 82 157 567 1566 2579 Fragments arranged by size: 2579 1566 834 567 157 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3910 4775 5073 3910 Size: 865 298 4622 Fragments arranged by size: 4622 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2292 4448 882 Size: 617 793 2156 2219 Fragments arranged by size: 2219 2156 793 617 BsaAI yAC'GTr Cuts at: 493 1204 2150 2551 3576 493 Size: 711 946 401 1025 2702 Fragments arranged by size: 2702 1025 946 711 401 BsaBI GATnn'nnATC Cuts at: 2310 5779 2310 Size: 3469 2316 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2103 3592 3974 4609 Size: 53 83 186 936 570 1489 382 635 Cuts at: 4609 275 Size: 1451 Fragments arranged by size: 1489 1451 936 635 570 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4218 4616 4890 5037 5448 4218 Size: 398 274 147 411 4555 Fragments arranged by size: 4555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5560 Fragments arranged by size: 5560 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5785 BseYI C'CCAG_C Cuts at: 4988 5484 4988 Size: 496 5289 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5212 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3235 3996 5024 1540 Size: 275 186 1234 761 1028 2301 Fragments arranged by size: 2301 1234 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3846 3931 5002 3846 Size: 85 1071 4629 Fragments arranged by size: 4629 1071 85 BsmI GAATG_Cn' Cuts at: 3296 3389 3296 Size: 93 5692 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2099 2575 3104 3846 3931 5002 Size: 399 277 32 476 529 742 85 1071 Cuts at: 5002 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 529 476 399 277 85 32 BspEI T'CCGG_A Cuts at: 5448 5448 Size: 5785 BspHI T'CATG_A Cuts at: 3674 5404 3674 Size: 1730 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2287 3140 844 Size: 1443 853 3489 Fragments arranged by size: 3489 1443 853 BsrBI CCG'CTC Cuts at: 1394 1823 3672 1394 Size: 429 1849 3507 Fragments arranged by size: 3507 1849 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4280 4454 65 Size: 1183 3032 174 1396 Fragments arranged by size: 3032 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2031 4429 4616 1700 Size: 110 221 2398 187 2869 Fragments arranged by size: 2869 2398 221 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5785 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5785 BssSI C'ACGA_G Cuts at: 3845 4857 3845 Size: 1012 4773 BstAPI GCAn_nnn'nTGC Cuts at: 794 2710 794 Size: 1916 3869 BstBI TT'CG_AA Cuts at: 4589 4589 Size: 5785 BstEII G'GTnAC_C Cuts at: 1184 2317 1184 Size: 1133 4652 BstXI CCAn_nnnn'nTGG Cuts at: 2842 2842 Size: 5785 BstYI r'GATC_y Cuts at: 1972 2338 3115 3482 3867 3884 5336 5422 5434 Size: 366 777 367 385 17 1452 86 12 Cuts at: 5434 5780 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 777 385 367 366 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5233 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5046 Fragments arranged by size: 5046 683 56 BtsI GCAGTG_nn' Cuts at: 2163 3301 4095 4115 2163 Size: 1138 794 20 3833 Fragments arranged by size: 3833 1138 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5750 DraI TTT'AAA Cuts at: 3089 3089 Size: 5785 DraIII CAC_nnn'GTG Cuts at: 2562 2562 Size: 5785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4792 809 Size: 507 3476 1802 Fragments arranged by size: 3476 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2553 2643 2961 Size: 54 1425 50 275 18 723 90 318 Cuts at: 2961 3232 4121 5750 8 Size: 271 889 1629 43 Fragments arranged by size: 1629 1425 889 723 318 275 271 90 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3232 1537 Size: 275 1420 4090 Fragments arranged by size: 4090 1420 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3066 3715 1353 Size: 1713 649 3423 Fragments arranged by size: 3423 1713 649 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4372 4746 4892 387 Size: 1064 138 2783 374 146 1280 Fragments arranged by size: 2783 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2155 2312 2516 2684 2879 3848 Size: 393 441 82 157 204 168 195 969 Cuts at: 3848 4445 5232 5500 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 969 787 597 441 393 268 204 195 168 157 82 EcoO109I rG'GnC_Cy Cuts at: 2452 2995 2452 Size: 543 5242 EcoRI G'AATT_C Cuts at: 2297 3094 3472 2297 Size: 797 378 4610 Fragments arranged by size: 4610 797 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2827 2827 Size: 5785 FspI TGC'GCA Cuts at: 4291 4291 Size: 5785 HaeII r_GCGC'y Cuts at: 1678 2106 4932 1678 Size: 428 2826 2531 Fragments arranged by size: 2826 2531 428 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 2698 2730 3065 Size: 32 135 32 1037 32 57 32 335 Cuts at: 3065 3097 4426 4458 4500 4532 1373 Size: 32 1329 32 42 32 2626 Fragments arranged by size: 2626 1329 1037 335 135 57 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2655 2851 3129 3375 669 Size: 988 281 717 196 278 246 3079 Fragments arranged by size: 3079 988 717 281 278 246 196 HindIII A'AGCT_T Cuts at: 748 2366 748 Size: 1618 4167 HpaI GTT'AAC Cuts at: 3375 3375 Size: 5785 KasI G'GCGC_C Cuts at: 2102 2102 Size: 5785 KpnI G_GTAC'C Cuts at: 3110 3110 Size: 5785 MfeI C'AATT_G Cuts at: 3384 3384 Size: 5785 MluI A'CGCG_T Cuts at: 5661 5661 Size: 5785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2244 4899 5083 1305 Size: 939 2655 184 2007 Fragments arranged by size: 2655 2007 939 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2555 10 Size: 54 1425 343 723 3240 Fragments arranged by size: 3240 1425 723 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2247 2529 3743 4102 4261 518 Size: 648 1081 282 1214 359 159 2042 Fragments arranged by size: 2042 1214 1081 648 359 282 159 MspA1I CmG'CkG Cuts at: 1606 2435 2900 3201 3878 5026 5271 1606 Size: 829 465 301 677 1148 245 2120 Fragments arranged by size: 2120 1148 829 677 465 301 245 NarI GG'CG_CC Cuts at: 2103 2103 Size: 5785 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5233 NdeI CA'TA_TG Cuts at: 387 2235 387 Size: 1848 3937 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5785 NotI GC'GGCC_GC Cuts at: 3232 3232 Size: 5785 NsiI A_TGCA'T Cuts at: 2601 2639 2601 Size: 38 5747 NspI r_CATG'y Cuts at: 1132 1229 2921 3143 4688 1132 Size: 97 1692 222 1545 2229 Fragments arranged by size: 2229 1692 1545 222 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5785 PciI A'CATG_T Cuts at: 4684 4684 Size: 5785 PflMI CCAn_nnn'nTGG Cuts at: 1108 2329 2562 1108 Size: 1221 233 4331 Fragments arranged by size: 4331 1221 233 PmeI GTTT'AAAC Cuts at: 3089 3089 Size: 5785 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3854 3886 5367 5399 3854 Size: 32 1481 32 4240 Fragments arranged by size: 4240 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2452 2995 2452 Size: 543 5242 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5785 PsiI TTA'TAA Cuts at: 3355 3355 Size: 5785 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2208 2240 2208 Size: 32 5753 PstI C_TGCA'G Cuts at: 830 3137 830 Size: 2307 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5785 PvuII CAG'CTG Cuts at: 2435 2435 Size: 5785 SalI G'TCGA_C Cuts at: 1936 2849 3127 1936 Size: 913 278 4594 Fragments arranged by size: 4594 913 278 SbfI CC_TGCA'GG Cuts at: 3137 3137 Size: 5785 ScaI AGT'ACT Cuts at: 4033 5745 4033 Size: 1712 4073 SfcI C'TryA_G Cuts at: 826 1045 2209 3133 4268 4949 5140 826 Size: 219 1164 924 1135 681 191 1471 Fragments arranged by size: 1471 1164 1135 924 681 219 191 SfoI GGC'GCC Cuts at: 2104 2104 Size: 5785 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5785 SmaI CCC'GGG Cuts at: 1872 3112 1872 Size: 1240 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3889 4790 5052 820 Size: 197 926 1946 901 262 1553 Fragments arranged by size: 1946 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3576 493 Size: 3083 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5785 SphI G_CATG'C Cuts at: 1132 3143 1132 Size: 2011 3774 SspI AAT'ATT Cuts at: 5 52 3709 5 Size: 47 3657 2081 Fragments arranged by size: 3657 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5785 StyI C'CwwG_G Cuts at: 513 1065 3224 513 Size: 552 2159 3074 Fragments arranged by size: 3074 2159 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3812 3829 3982 4167 4612 325 Size: 1255 2232 17 153 185 445 1498 Fragments arranged by size: 2232 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4031 5743 96 Size: 274 80 33 51 1443 2054 1712 138 Fragments arranged by size: 2054 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5745 5777 5745 Size: 32 5753 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5785 XbaI T'CTAG_A Cuts at: 3121 3121 Size: 5785 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5785 XmaI C'CCGG_G Cuts at: 1870 3110 1870 Size: 1240 4545 XmnI GAAnn'nnTTC Cuts at: 3914 3914 Size: 5785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3593 276 Size: 53 83 186 936 2059 2468 Fragments arranged by size: 2468 2059 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI