(Circular) (Six-base) MAPSORT of: pFN21AB3651.seq Check: 7013 from: 1 to: 7717 pFN21AB3651 7717 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3088 3088 Size: 7717 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5527 278 Size: 53 83 186 936 3991 2468 Fragments arranged by size: 3991 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5060 1937 Size: 3123 4594 Acc65I G'GTAC_C Cuts at: 5038 5038 Size: 7717 AclI AA'CG_TT Cuts at: 1563 5844 6217 1563 Size: 4281 373 3063 Fragments arranged by size: 4281 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3419 4104 4298 4418 4535 5780 7164 7432 Size: 1787 685 194 120 117 1245 1384 268 Cuts at: 7432 1632 Size: 1917 Fragments arranged by size: 1917 1787 1384 1245 685 268 194 120 117 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7520 AflIII A'CryG_T Cuts at: 1203 3811 6616 7593 1203 Size: 2608 2805 977 1327 Fragments arranged by size: 2805 2608 1327 977 AgeI A'CCGG_T Cuts at: 6548 6548 Size: 7717 AhdI GACnn_n'nnGTC Cuts at: 6446 6446 Size: 7717 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7717 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2163 3216 1184 Size: 979 1053 5685 Fragments arranged by size: 5685 1053 979 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2018 2050 2402 2434 2018 Size: 32 352 32 7301 Fragments arranged by size: 7301 352 32 32 AlwNI CAG_nnn'CTG Cuts at: 2122 2969 3656 4276 5460 7032 2122 Size: 847 687 620 1184 1572 2807 Fragments arranged by size: 2807 1572 1184 847 687 620 ApaI G_GGCC'C Cuts at: 4973 4973 Size: 7717 ApaLI G'TGCA_C Cuts at: 3744 3887 5774 6930 3744 Size: 143 1887 1156 4531 Fragments arranged by size: 4531 1887 1156 143 ApoI r'AATT_y Cuts at: 1493 4886 5026 5252 5404 7513 1493 Size: 3393 140 226 152 2109 1697 Fragments arranged by size: 3393 2109 1697 226 152 140 AseI AT'TA_AT Cuts at: 160 1032 6271 160 Size: 872 5239 1606 Fragments arranged by size: 5239 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7717 AvaI C'yCGr_G Cuts at: 1870 1943 3669 5042 1870 Size: 73 1726 1373 4545 Fragments arranged by size: 4545 1726 1373 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2919 2952 2919 Size: 33 7684 BamHI G'GATC_C Cuts at: 5047 5414 5047 Size: 367 7350 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2572 5038 6493 618 Size: 325 217 627 785 2466 1455 1842 Fragments arranged by size: 2466 1842 1455 785 627 325 217 BanII G_rGCy'C Cuts at: 1391 2369 4973 5036 1391 Size: 978 2604 63 4072 Fragments arranged by size: 4072 2604 978 63 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3432 4647 928 Size: 971 1533 1215 3998 Fragments arranged by size: 3998 1533 1215 971 BbvCI CC'TCA_GC Cuts at: 2650 3348 2650 Size: 698 7019 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4832 4866 5908 5942 1903 Size: 34 2895 34 1042 34 3678 Fragments arranged by size: 3678 2895 1042 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5609 6819 5609 Size: 1210 6507 BclI T'GATC_A Cuts at: 1548 1548 Size: 7717 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3017 3710 6328 Size: 107 122 71 631 605 1345 693 2618 Cuts at: 6328 136 Size: 1525 Fragments arranged by size: 2618 1525 1345 693 631 605 122 107 71 BglII A'GATC_T Cuts at: 4983 7712 4983 Size: 2729 4988 BlpI GC'TnA_GC Cuts at: 3106 5134 3106 Size: 2028 5689 Bme1580I G_kGCm'C Cuts at: 1790 3748 3761 3891 4973 5778 6934 1790 Size: 1958 13 130 1082 805 1156 2573 Fragments arranged by size: 2573 1958 1156 1082 805 130 13 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7717 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2414 3385 5448 Size: 416 53 83 399 24 997 971 2063 Cuts at: 5448 6406 442 Size: 958 1753 Fragments arranged by size: 2063 1753 997 971 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7717 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2044 2044 Size: 7717 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2162 2613 2682 3152 3251 3981 6377 1239 Size: 923 451 69 470 99 730 2396 2579 Fragments arranged by size: 2579 2396 923 730 470 451 99 69 Bpu10I CC'TnA_GC Cuts at: 2056 2650 3348 3474 2056 Size: 594 698 126 6299 Fragments arranged by size: 6299 698 594 126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2640 4964 4991 5842 6707 7005 2640 Size: 2324 27 851 865 298 3352 Fragments arranged by size: 3352 2324 865 851 298 27 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5006 6380 882 Size: 617 3507 1374 2219 Fragments arranged by size: 3507 2219 1374 617 BsaAI yAC'GTr Cuts at: 493 1204 5508 493 Size: 711 4304 2702 Fragments arranged by size: 4304 2702 711 BsaBI GATnn'nnATC Cuts at: 2610 3203 7711 2610 Size: 593 4508 2616 Fragments arranged by size: 4508 2616 593 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5524 5906 6541 275 Size: 53 83 186 936 3991 382 635 1451 Fragments arranged by size: 3991 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4771 6150 6548 6822 6969 7380 4771 Size: 1379 398 274 147 411 5108 Fragments arranged by size: 5108 1379 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2402 2432 3775 3805 1178 Size: 30 165 30 999 30 1343 30 5090 Fragments arranged by size: 5090 1343 999 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3706 3829 3956 3959 4579 4847 1186 Size: 2520 123 127 3 620 268 4056 Fragments arranged by size: 4056 2520 620 268 127 123 3 BseYI C'CCAG_C Cuts at: 2199 2337 2467 3086 3240 3396 3558 4721 4814 Size: 138 130 619 154 156 162 1163 93 Cuts at: 4814 4966 6920 7416 2199 Size: 152 1954 496 2500 Fragments arranged by size: 2500 1954 1163 619 496 162 156 154 152 138 130 93 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2135 2960 3024 3710 3818 1163 Size: 573 399 825 64 686 108 5062 Fragments arranged by size: 5062 825 686 573 399 108 64 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2334 5167 5928 6956 1540 Size: 275 186 333 2833 761 1028 2301 Fragments arranged by size: 2833 2301 1028 761 333 275 186 BsiHKAI G_wGCw'C Cuts at: 2369 3748 3891 5778 5863 6934 2369 Size: 1379 143 1887 85 1071 3152 Fragments arranged by size: 3152 1887 1379 1071 143 85 BsmI GAATG_Cn' Cuts at: 4078 5228 5321 4078 Size: 1150 93 6474 Fragments arranged by size: 6474 1150 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2369 3748 3761 3891 4973 5036 5778 Size: 399 579 1379 13 130 1082 63 742 Cuts at: 5778 5863 6934 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1379 1082 1071 742 579 399 130 85 63 13 BspEI T'CCGG_A Cuts at: 7380 7380 Size: 7717 BspHI T'CATG_A Cuts at: 5606 7336 5606 Size: 1730 5987 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2473 3088 3367 5072 844 Size: 1629 615 279 1705 3489 Fragments arranged by size: 3489 1705 1629 615 279 BsrBI CCG'CTC Cuts at: 1394 1823 5604 1394 Size: 429 3781 3507 Fragments arranged by size: 3781 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4596 6212 6386 65 Size: 1183 3348 1616 174 1396 Fragments arranged by size: 3348 1616 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3231 6361 6548 1700 Size: 110 1421 3130 187 2869 Fragments arranged by size: 3130 2869 1421 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7717 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7717 BssSI C'ACGA_G Cuts at: 5777 6789 5777 Size: 1012 6705 BstAPI GCAn_nnn'nTGC Cuts at: 794 2122 2131 2572 2947 3002 3886 794 Size: 1328 9 441 375 55 884 4625 Fragments arranged by size: 4625 1328 884 441 375 55 9 BstBI TT'CG_AA Cuts at: 6521 6521 Size: 7717 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7717 BstXI CCAn_nnnn'nTGG Cuts at: 3740 3740 Size: 7717 BstYI r'GATC_y Cuts at: 1972 3123 3159 4983 5047 5414 5799 5816 7268 Size: 1151 36 1824 64 367 385 17 1452 Cuts at: 7268 7354 7366 7712 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1824 1452 1151 385 367 346 86 64 36 17 12 Bsu36I CC'TnA_GG Cuts at: 2893 2893 Size: 7717 BtgI C'CryG_G Cuts at: 513 1065 2004 2838 4143 513 Size: 552 939 834 1305 4087 Fragments arranged by size: 4087 1305 939 834 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6978 Fragments arranged by size: 6978 683 56 BtsI GCAGTG_nn' Cuts at: 2118 2129 2821 3339 3746 4096 5233 6027 6047 Size: 11 692 518 407 350 1137 794 20 Cuts at: 6047 2118 Size: 3788 Fragments arranged by size: 3788 1137 794 692 518 407 350 20 11 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7717 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7682 DraI TTT'AAA Cuts at: 5021 5021 Size: 7717 DraIII CAC_nnn'GTG Cuts at: 2870 2870 Size: 7717 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6724 809 Size: 507 5408 1802 Fragments arranged by size: 5408 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2319 2331 2459 Size: 54 1425 50 275 18 489 12 128 Cuts at: 2459 3037 5164 6053 7682 8 Size: 578 2127 889 1629 43 Fragments arranged by size: 2127 1629 1425 889 578 489 275 128 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2331 5164 1537 Size: 275 519 2833 4090 Fragments arranged by size: 4090 2833 519 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3459 4025 4511 5647 1353 Size: 2106 566 486 1136 3423 Fragments arranged by size: 3423 2106 1136 566 486 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6304 6678 6824 387 Size: 1064 138 4715 374 146 1280 Fragments arranged by size: 4715 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2367 2367 Size: 7717 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2162 2613 2682 3152 3251 3419 3981 Size: 393 530 451 69 470 99 168 562 Cuts at: 3981 4104 4298 4418 4535 5780 6377 7164 7432 Size: 123 194 120 117 1245 597 787 268 Cuts at: 7432 1239 Size: 1524 Fragments arranged by size: 1524 1245 787 597 562 530 470 451 393 268 194 168 123 120 117 99 69 EcoNI CCTnn'n_nnAGG Cuts at: 2480 2726 2892 3531 2480 Size: 246 166 639 6666 Fragments arranged by size: 6666 639 246 166 EcoO109I rG'GnC_Cy Cuts at: 2660 2743 3361 3379 3859 4915 4970 2660 Size: 83 618 18 480 1056 55 5407 Fragments arranged by size: 5407 1056 618 480 83 55 18 EcoRI G'AATT_C Cuts at: 5026 5404 5026 Size: 378 7339 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4958 4958 Size: 7717 FspI TGC'GCA Cuts at: 6223 6223 Size: 7717 HaeII r_GCGC'y Cuts at: 1678 6864 1678 Size: 5186 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2561 2593 3775 3807 3942 Size: 32 135 32 989 32 1182 32 135 Cuts at: 3942 3944 3974 3976 4315 4319 4347 4349 4351 Size: 2 30 2 339 4 28 2 2 Cuts at: 4351 4381 6358 6390 6432 6464 1373 Size: 30 1977 32 42 32 2626 Fragments arranged by size: 2626 1977 1182 989 339 135 135 42 32 32 32 32 32 32 30 30 28 4 2 2 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 5061 5307 669 Size: 988 281 3123 246 3079 Fragments arranged by size: 3123 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7717 HpaI GTT'AAC Cuts at: 5307 5307 Size: 7717 KpnI G_GTAC'C Cuts at: 5042 5042 Size: 7717 MfeI C'AATT_G Cuts at: 2235 5316 2235 Size: 3081 4636 MluI A'CGCG_T Cuts at: 7593 7593 Size: 7717 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2483 4337 4715 4802 6831 7015 1305 Size: 1178 1854 378 87 2029 184 2007 Fragments arranged by size: 2029 2007 1854 1178 378 184 87 MscI TGG'CCA Cuts at: 10 64 1489 1832 2321 3039 10 Size: 54 1425 343 489 718 4688 Fragments arranged by size: 4688 1425 718 489 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2604 5675 6034 6193 518 Size: 648 1368 70 3071 359 159 2042 Fragments arranged by size: 3071 2042 1368 648 359 159 70 MspA1I CmG'CkG Cuts at: 1606 2137 2203 2467 3554 3646 3653 5133 5810 Size: 531 66 264 1087 92 7 1480 677 Cuts at: 5810 6958 7203 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1480 1148 1087 677 531 264 245 92 66 7 NcoI C'CATG_G Cuts at: 513 1065 2004 2838 4143 513 Size: 552 939 834 1305 4087 Fragments arranged by size: 4087 1305 939 834 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7717 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7717 NotI GC'GGCC_GC Cuts at: 5164 5164 Size: 7717 NspI r_CATG'y Cuts at: 1132 1229 3815 5075 6620 1132 Size: 97 2586 1260 1545 2229 Fragments arranged by size: 2586 2229 1545 1260 97 PasI CC'CwG_GG Cuts at: 1348 2497 2831 3776 1348 Size: 1149 334 945 5289 Fragments arranged by size: 5289 1149 945 334 PciI A'CATG_T Cuts at: 3811 6616 3811 Size: 2805 4912 PflMI CCAn_nnn'nTGG Cuts at: 1108 3415 3913 4821 1108 Size: 2307 498 908 4004 Fragments arranged by size: 4004 2307 908 498 PfoI T'CCnGG_A Cuts at: 2590 2698 4233 2590 Size: 108 1535 6074 Fragments arranged by size: 6074 1535 108 PmeI GTTT'AAAC Cuts at: 5021 5021 Size: 7717 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2402 2434 5786 5818 7299 7331 2402 Size: 32 3352 32 1481 32 2788 Fragments arranged by size: 3352 2788 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2660 3361 3379 3859 2660 Size: 701 18 480 6518 Fragments arranged by size: 6518 701 480 18 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7717 PsiI TTA'TAA Cuts at: 5287 5287 Size: 7717 PspOMI G'GGCC_C Cuts at: 4969 4969 Size: 7717 PstI C_TGCA'G Cuts at: 830 2136 2560 2956 3753 5069 830 Size: 1306 424 396 797 1316 3478 Fragments arranged by size: 3478 1316 1306 797 424 396 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7717 PvuII CAG'CTG Cuts at: 2203 2467 3554 3646 3653 2203 Size: 264 1087 92 7 6267 Fragments arranged by size: 6267 1087 264 92 7 SacI G_AGCT'C Cuts at: 2369 2369 Size: 7717 SalI G'TCGA_C Cuts at: 1936 5059 1936 Size: 3123 4594 SapI GCTCTTCn'nnn_ Cuts at: 4025 4025 Size: 7717 SbfI CC_TGCA'GG Cuts at: 5069 5069 Size: 7717 ScaI AGT'ACT Cuts at: 4409 5965 7677 4409 Size: 1556 1712 4449 Fragments arranged by size: 4449 1712 1556 SfcI C'TryA_G Cuts at: 826 1045 2132 2556 2952 3507 3749 4298 4495 Size: 219 1087 424 396 555 242 549 197 Cuts at: 4495 5065 6200 6881 7072 826 Size: 570 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 681 570 555 549 424 396 242 219 197 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3710 3710 Size: 7717 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7717 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7717 SmaI CCC'GGG Cuts at: 1872 3671 5044 1872 Size: 1799 1373 4545 Fragments arranged by size: 4545 1799 1373 SmlI C'TyrA_G Cuts at: 820 1017 1943 2655 4979 5006 5821 6722 6984 Size: 197 926 712 2324 27 815 901 262 Cuts at: 6984 820 Size: 1553 Fragments arranged by size: 2324 1553 926 901 815 712 262 197 27 SnaBI TAC'GTA Cuts at: 493 5508 493 Size: 5015 2702 SpeI A'CTAG_T Cuts at: 152 4096 152 Size: 3944 3773 SphI G_CATG'C Cuts at: 1132 5075 1132 Size: 3943 3774 SspI AAT'ATT Cuts at: 5 52 5641 5 Size: 47 5589 2081 Fragments arranged by size: 5589 2081 47 StuI AGG'CCT Cuts at: 1517 2306 2648 2732 1517 Size: 789 342 84 6502 Fragments arranged by size: 6502 789 342 84 StyI C'CwwG_G Cuts at: 513 1065 2004 2838 3987 4143 4861 5156 513 Size: 552 939 834 1149 156 718 295 3074 Fragments arranged by size: 3074 1149 939 834 718 552 295 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2315 2891 4151 4223 5744 5761 5914 Size: 1255 735 576 1260 72 1521 17 153 Cuts at: 5914 6099 6544 325 Size: 185 445 1498 Fragments arranged by size: 1521 1498 1260 1255 735 576 445 185 153 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4407 5963 7675 Size: 274 80 33 51 1443 2430 1556 1712 Cuts at: 7675 96 Size: 138 Fragments arranged by size: 2430 1712 1556 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3221 3253 3345 3377 3561 3593 7677 7709 3221 Size: 32 92 32 184 32 4084 32 3229 Fragments arranged by size: 4084 3229 184 92 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7717 XbaI T'CTAG_A Cuts at: 4151 5053 4151 Size: 902 6815 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7717 XmaI C'CCGG_G Cuts at: 1870 3669 5042 1870 Size: 1799 1373 4545 Fragments arranged by size: 4545 1799 1373 XmnI GAAnn'nnTTC Cuts at: 5846 5846 Size: 7717 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5525 276 Size: 53 83 186 936 3991 2468 Fragments arranged by size: 3991 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfoI SrfI SwaI XcmI