(Circular) (Six-base) MAPSORT of: pFN21AB3513.seq Check: 7686 from: 1 to: 7774 pFN21AB3513 7774 bp With 182 enzymes: SgfI * February 27, 2009 14:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4763 4763 Size: 7774 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5584 278 Size: 53 83 186 936 4048 2468 Fragments arranged by size: 4048 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2781 5117 1937 Size: 844 2336 4594 Fragments arranged by size: 4594 2336 844 Acc65I G'GTAC_C Cuts at: 5095 5095 Size: 7774 AclI AA'CG_TT Cuts at: 1563 5901 6274 1563 Size: 4338 373 3063 Fragments arranged by size: 4338 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 3231 3723 5837 7221 7489 1632 Size: 497 1102 492 2114 1384 268 1917 Fragments arranged by size: 2114 1917 1384 1102 497 492 268 AflII C'TTAA_G Cuts at: 820 1017 3332 820 Size: 197 2315 5262 Fragments arranged by size: 5262 2315 197 AflIII A'CryG_T Cuts at: 1203 3174 6673 7650 1203 Size: 1971 3499 977 1327 Fragments arranged by size: 3499 1971 1327 977 AgeI A'CCGG_T Cuts at: 6605 6605 Size: 7774 AhdI GACnn_n'nnGTC Cuts at: 6503 6503 Size: 7774 AleI CACnn'nnGTG Cuts at: 1166 2894 4005 1166 Size: 1728 1111 4935 Fragments arranged by size: 4935 1728 1111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2650 1184 Size: 1466 6308 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2862 2894 2862 Size: 32 7742 AlwNI CAG_nnn'CTG Cuts at: 3592 4591 5517 7089 3592 Size: 999 926 1572 4277 Fragments arranged by size: 4277 1572 999 926 ApaLI G'TGCA_C Cuts at: 5831 6987 5831 Size: 1156 6618 ApoI r'AATT_y Cuts at: 1493 2598 3733 4828 5083 5309 5461 7570 1493 Size: 1105 1135 1095 255 226 152 2109 1697 Fragments arranged by size: 2109 1697 1135 1105 1095 255 226 152 AseI AT'TA_AT Cuts at: 160 1032 6328 160 Size: 872 5296 1606 Fragments arranged by size: 5296 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7774 AvaI C'yCGr_G Cuts at: 1870 1943 4358 5099 1870 Size: 73 2415 741 4545 Fragments arranged by size: 4545 2415 741 73 AvrII C'CTAG_G Cuts at: 3661 3661 Size: 7774 BamHI G'GATC_C Cuts at: 3543 5104 5471 3543 Size: 1561 367 5846 Fragments arranged by size: 5846 1561 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2759 3530 5095 6550 618 Size: 325 217 627 972 771 1565 1455 1842 Fragments arranged by size: 1842 1565 1455 972 771 627 325 217 BanII G_rGCy'C Cuts at: 1391 3507 3768 5093 1391 Size: 2116 261 1325 4072 Fragments arranged by size: 4072 2116 1325 261 BbeI G_GCGC'C Cuts at: 3534 3534 Size: 7774 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3936 4707 4828 928 Size: 971 2037 771 121 3874 Fragments arranged by size: 3874 2037 971 771 121 BbvCI CC'TCA_GC Cuts at: 2502 4089 2502 Size: 1587 6187 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5965 5999 1903 Size: 34 4028 34 3678 Fragments arranged by size: 4028 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4956 5666 6876 4956 Size: 710 1210 5854 Fragments arranged by size: 5854 1210 710 BclI T'GATC_A Cuts at: 1548 2131 4551 1548 Size: 583 2420 4771 Fragments arranged by size: 4771 2420 583 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5046 6385 136 Size: 107 122 71 631 605 3374 1339 1525 Fragments arranged by size: 3374 1525 1339 631 605 122 107 71 BglII A'GATC_T Cuts at: 4860 7769 4860 Size: 2909 4865 BlpI GC'TnA_GC Cuts at: 5191 5191 Size: 7774 Bme1580I G_kGCm'C Cuts at: 1790 4445 5835 6991 1790 Size: 2655 1390 1156 2573 Fragments arranged by size: 2655 2573 1390 1156 BmgBI CAC'GTC Cuts at: 1318 2024 3114 1318 Size: 706 1090 5978 Fragments arranged by size: 5978 1090 706 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2796 3950 4418 Size: 416 53 83 399 24 1379 1154 468 Cuts at: 4418 4681 5505 6463 442 Size: 263 824 958 1753 Fragments arranged by size: 1753 1379 1154 958 824 468 416 399 263 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7774 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2673 2735 4155 4305 6434 1239 Size: 1434 62 1420 150 2129 2579 Fragments arranged by size: 2579 2129 1434 1420 150 62 Bpu10I CC'TnA_GC Cuts at: 2502 4089 2502 Size: 1587 6187 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2200 2963 2978 5899 6764 7062 2200 Size: 763 15 2921 865 298 2912 Fragments arranged by size: 2921 2912 865 763 298 15 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2586 2691 3092 3852 3914 6437 882 Size: 617 1087 105 401 760 62 2523 2219 Fragments arranged by size: 2523 2219 1087 760 617 401 105 62 BsaAI yAC'GTr Cuts at: 493 1204 2768 5565 493 Size: 711 1564 2797 2702 Fragments arranged by size: 2797 2702 1564 711 BsaBI GATnn'nnATC Cuts at: 2194 7768 2194 Size: 5574 2200 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3531 3612 5038 5581 Size: 53 83 186 936 1998 81 1426 543 Cuts at: 5581 5963 6598 275 Size: 382 635 1451 Fragments arranged by size: 1998 1451 1426 936 635 543 382 186 83 81 53 BsaWI w'CCGG_w Cuts at: 3156 6207 6605 6879 7026 7437 3156 Size: 3051 398 274 147 411 3493 Fragments arranged by size: 3493 3051 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2236 2266 2671 2701 3642 Size: 30 165 30 833 30 405 30 941 Cuts at: 3642 3672 4010 4040 1178 Size: 30 338 30 4912 Fragments arranged by size: 4912 941 833 405 338 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2604 2641 3560 3614 1186 Size: 1418 37 919 54 5346 Fragments arranged by size: 5346 1418 919 54 37 BseYI C'CCAG_C Cuts at: 3637 5049 6977 7473 3637 Size: 1412 1928 496 3938 Fragments arranged by size: 3938 1928 1412 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2226 4811 1163 Size: 573 490 2585 4126 Fragments arranged by size: 4126 2585 573 490 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3798 5224 5985 7013 1540 Size: 275 186 1797 1426 761 1028 2301 Fragments arranged by size: 2301 1797 1426 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5835 5920 6991 5835 Size: 85 1071 6618 Fragments arranged by size: 6618 1071 85 BsmI GAATG_Cn' Cuts at: 5285 5378 5285 Size: 93 7681 BsmBI CGTCTCn'nnnn_ Cuts at: 2711 2711 Size: 7774 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3507 3768 4445 5093 5835 5920 6991 Size: 399 1717 261 677 648 742 85 1071 Cuts at: 6991 1391 Size: 2174 Fragments arranged by size: 2174 1717 1071 742 677 648 399 261 85 BspEI T'CCGG_A Cuts at: 7437 7437 Size: 7774 BspHI T'CATG_A Cuts at: 4497 5663 7393 4497 Size: 1166 1730 4878 Fragments arranged by size: 4878 1730 1166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3269 4763 5129 844 Size: 2425 1494 366 3489 Fragments arranged by size: 3489 2425 1494 366 BsrBI CCG'CTC Cuts at: 1394 1823 4546 5661 1394 Size: 429 2723 1115 3507 Fragments arranged by size: 3507 2723 1115 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2727 2853 3922 3933 4329 6269 6443 Size: 1183 1479 126 1069 11 396 1940 174 Cuts at: 6443 65 Size: 1396 Fragments arranged by size: 1940 1479 1396 1183 1069 396 174 126 11 BsrFI r'CCGG_y Cuts at: 1700 1810 6418 6605 1700 Size: 110 4608 187 2869 Fragments arranged by size: 4608 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7774 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7774 BssSI C'ACGA_G Cuts at: 2202 5834 6846 2202 Size: 3632 1012 3130 Fragments arranged by size: 3632 3130 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3353 794 Size: 2559 5215 BstBI TT'CG_AA Cuts at: 2521 6578 2521 Size: 4057 3717 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7774 BstXI CCAn_nnnn'nTGG Cuts at: 3055 3895 4468 3055 Size: 840 573 6361 Fragments arranged by size: 6361 840 573 BstYI r'GATC_y Cuts at: 1972 2214 2569 3543 4860 5104 5471 5856 5873 Size: 242 355 974 1317 244 367 385 17 Cuts at: 5873 7325 7411 7423 7769 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1317 974 385 367 355 346 244 242 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3574 3788 3810 3924 513 Size: 552 939 1570 214 22 114 4363 Fragments arranged by size: 4363 1570 939 552 214 114 22 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7035 Fragments arranged by size: 7035 683 56 BtsI GCAGTG_nn' Cuts at: 3303 4012 4724 4799 5290 6084 6104 3303 Size: 709 712 75 491 794 20 4973 Fragments arranged by size: 4973 794 712 709 491 75 20 ClaI AT'CG_AT Cuts at: 1554 3744 1554 Size: 2190 5584 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7739 DraI TTT'AAA Cuts at: 5078 5078 Size: 7774 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3106 6781 809 Size: 507 1790 3675 1802 Fragments arranged by size: 3675 1802 1790 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3015 3795 5221 Size: 54 1425 50 275 18 1185 780 1426 Cuts at: 5221 6110 7739 8 Size: 889 1629 43 Fragments arranged by size: 1629 1426 1425 1185 889 780 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3795 5221 1537 Size: 275 1983 1426 4090 Fragments arranged by size: 4090 1983 1426 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2364 4496 4878 4995 5704 1353 Size: 1011 2132 382 117 709 3423 Fragments arranged by size: 3423 2132 1011 709 382 117 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6361 6735 6881 387 Size: 1064 138 4772 374 146 1280 Fragments arranged by size: 4772 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2673 2735 3231 3723 4155 4305 Size: 393 497 544 62 496 492 432 150 Cuts at: 4305 5837 6434 7221 7489 1239 Size: 1532 597 787 268 1524 Fragments arranged by size: 1532 1524 787 597 544 497 496 492 432 393 268 150 62 EcoNI CCTnn'n_nnAGG Cuts at: 3596 3596 Size: 7774 EcoO109I rG'GnC_Cy Cuts at: 3643 4080 4588 3643 Size: 437 508 6829 Fragments arranged by size: 6829 508 437 EcoRI G'AATT_C Cuts at: 2598 4828 5083 5461 2598 Size: 2230 255 378 4911 Fragments arranged by size: 4911 2230 378 255 FspI TGC'GCA Cuts at: 6280 6280 Size: 7774 HaeII r_GCGC'y Cuts at: 1678 3534 6921 1678 Size: 1856 3387 2531 Fragments arranged by size: 3387 2531 1856 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2636 2668 2671 2703 3202 Size: 32 135 32 1064 32 3 32 499 Cuts at: 3202 3234 3642 3674 3769 3801 4010 4042 4072 Size: 32 408 32 95 32 209 32 30 Cuts at: 4072 4104 6415 6447 6489 6521 1373 Size: 32 2311 32 42 32 2626 Fragments arranged by size: 2626 2311 1064 499 408 209 135 95 42 32 32 32 32 32 32 32 32 32 32 32 30 3 HincII GTy'rAC Cuts at: 669 1657 1938 5118 5364 669 Size: 988 281 3180 246 3079 Fragments arranged by size: 3180 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7774 HpaI GTT'AAC Cuts at: 5364 5364 Size: 7774 KasI G'GCGC_C Cuts at: 3530 3530 Size: 7774 KpnI G_GTAC'C Cuts at: 5099 5099 Size: 7774 MfeI C'AATT_G Cuts at: 2746 4275 5373 2746 Size: 1529 1098 5147 Fragments arranged by size: 5147 1529 1098 MluI A'CGCG_T Cuts at: 7650 7650 Size: 7774 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1999 6888 7072 1305 Size: 694 4889 184 2007 Fragments arranged by size: 4889 2007 694 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3017 10 Size: 54 1425 343 1185 4767 Fragments arranged by size: 4767 1425 1185 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2801 2894 3773 4005 4106 4448 5732 Size: 648 1635 93 879 232 101 342 1284 Cuts at: 5732 6091 6250 518 Size: 359 159 2042 Fragments arranged by size: 2042 1635 1284 879 648 359 342 232 159 101 93 MspA1I CmG'CkG Cuts at: 1606 2045 2210 4036 4683 5190 5867 7015 7260 Size: 439 165 1826 647 507 677 1148 245 Cuts at: 7260 1606 Size: 2120 Fragments arranged by size: 2120 1826 1148 677 647 507 439 245 165 NarI GG'CG_CC Cuts at: 3531 3531 Size: 7774 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6283 Fragments arranged by size: 6283 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7774 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7774 NotI GC'GGCC_GC Cuts at: 5221 5221 Size: 7774 NspI r_CATG'y Cuts at: 1132 1229 3178 5132 6677 1132 Size: 97 1949 1954 1545 2229 Fragments arranged by size: 2229 1954 1949 1545 97 PasI CC'CwG_GG Cuts at: 1348 3975 4591 1348 Size: 2627 616 4531 Fragments arranged by size: 4531 2627 616 PciI A'CATG_T Cuts at: 3174 6673 3174 Size: 3499 4275 PflMI CCAn_nnn'nTGG Cuts at: 1108 4591 1108 Size: 3483 4291 PmeI GTTT'AAAC Cuts at: 5078 5078 Size: 7774 PmlI CAC'GTG Cuts at: 2768 2768 Size: 7774 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3133 3165 5843 5875 7356 7388 3133 Size: 32 2678 32 1481 32 3519 Fragments arranged by size: 3519 2678 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4080 4080 Size: 7774 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7774 PsiI TTA'TAA Cuts at: 5344 5344 Size: 7774 PstI C_TGCA'G Cuts at: 830 2044 2863 5126 830 Size: 1214 819 2263 3478 Fragments arranged by size: 3478 2263 1214 819 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7774 PvuII CAG'CTG Cuts at: 2045 2210 4036 2045 Size: 165 1826 5783 Fragments arranged by size: 5783 1826 165 SalI G'TCGA_C Cuts at: 1936 5116 1936 Size: 3180 4594 SbfI CC_TGCA'GG Cuts at: 5126 5126 Size: 7774 ScaI AGT'ACT Cuts at: 6022 7734 6022 Size: 1712 6062 SexAI A'CCwGG_T Cuts at: 4961 4961 Size: 7774 SfcI C'TryA_G Cuts at: 826 1045 2040 2364 2859 5122 6257 6938 7129 Size: 219 995 324 495 2263 1135 681 191 Cuts at: 7129 826 Size: 1471 Fragments arranged by size: 2263 1471 1135 995 681 495 324 219 191 SfoI GGC'GCC Cuts at: 3532 3532 Size: 7774 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7774 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7774 SmaI CCC'GGG Cuts at: 1872 5101 1872 Size: 3229 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2179 2942 2957 3332 5878 6779 Size: 197 926 236 763 15 375 2546 901 Cuts at: 6779 7041 820 Size: 262 1553 Fragments arranged by size: 2546 1553 926 901 763 375 262 236 197 15 SnaBI TAC'GTA Cuts at: 493 5565 493 Size: 5072 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7774 SphI G_CATG'C Cuts at: 1132 5132 1132 Size: 4000 3774 SspI AAT'ATT Cuts at: 5 52 5698 5 Size: 47 5646 2081 Fragments arranged by size: 5646 2081 47 StuI AGG'CCT Cuts at: 1517 3989 1517 Size: 2472 5302 StyI C'CwwG_G Cuts at: 513 1065 2004 3102 3661 3984 5213 513 Size: 552 939 1098 559 323 1229 3074 Fragments arranged by size: 3074 1229 1098 939 559 552 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2390 2610 2900 4822 4916 5041 5801 Size: 1255 810 220 290 1922 94 125 760 Cuts at: 5801 5818 5971 6156 6601 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1922 1498 1255 810 760 445 290 220 185 153 125 94 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2946 3997 6020 Size: 274 80 33 51 1443 969 1051 2023 Cuts at: 6020 7732 96 Size: 1712 138 Fragments arranged by size: 2023 1712 1443 1051 969 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2236 2268 7734 7766 2236 Size: 32 5466 32 2244 Fragments arranged by size: 5466 2244 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2683 4805 5012 1316 Size: 1367 2122 207 4078 Fragments arranged by size: 4078 2122 1367 207 XbaI T'CTAG_A Cuts at: 5110 5110 Size: 7774 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7774 XmaI C'CCGG_G Cuts at: 1870 5099 1870 Size: 3229 4545 XmnI GAAnn'nnTTC Cuts at: 3696 4634 5903 3696 Size: 938 1269 5567 Fragments arranged by size: 5567 1269 938 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5582 276 Size: 53 83 186 936 4048 2468 Fragments arranged by size: 4048 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI XcmI