Restriction map for
pFN21AB3512
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AspI
AvaI AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfaI BlnI BlpI BpmI
Bpu1102I BsaAI BsaHI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FseI HgaI Hin1I HindIII HpaI
HphI KasI KpnI Ksp632I KspI
MaeI MaeII MluI MluNI MroI
MscI MslI MspA1I MvnI NaeI
NarI NciI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
TaqI Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AcsI 176 GAATTC [GA]^AATT[CT]
AflII 415 CTTAAG C^TTAAG
AlwI 1156 GGATC GGATC(4/5)
ApoI 176 GAATTC [GA]^AATT[CT]
AseI 952 ATTAAT AT^TAAT
AsnI 952 ATTAAT AT^TAAT
AvaII 354 GGACC G^G[AT]CC
AviII 717 TGCGCA TGC^GCA
BanII 40 GAGCCC G[GA]GC[CT]^C
BfrI 415 CTTAAG C^TTAAG
BglII 411 AGATCT A^GATCT
BmyI 40 GAGCCC G[AGT]GC[ACT]^C
BsaBI 1157 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 328 GGTCTC GGTCTC(1/5)
BsmFI 70 GGGAC GGGAC(10/14)
Bsp1286I 40 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 592 TGTACA T^GTACA
BspMI 399 GCAGGT ACCTGC(4/8)
BsrGI 592 TGTACA T^GTACA
BstAPI 1162 GCATCCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 18 GGTAACC G^GT[ACGT]ACC
BstPI 18 GGTAACC G^GT[ACGT]ACC
BstYI 411 AGATCT [GA]^GATC[CT]
EcoO65I 18 GGTAACC G^GT[ACGT]ACC
EcoRI 176 GAATTC G^AATTC
FauI 621 GCGGG CCCGC(4/6)
FspI 717 TGCGCA TGC^GCA
HaeII 587 GGCGCT [GA]GCGC^[CT]
HapII 162 CCGG C^CGG
HincII 223 GTCAAC GT[CT]^[GA]AC
HindII 223 GTCAAC GT[CT]^[GA]AC
HpaII 162 CCGG C^CGG
MamI 1157 GATCAGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1145 CAATTG C^AATTG
MflI 411 AGATCT [GA]^GATC[CT]
MspI 162 CCGG C^CGG
MunI 1145 CAATTG C^AATTG
NcoI 334 CCATGG C^CATGG
NdeI 496 CATATG CA^TATG
NlaIV 1083 GGAGCC GG[ACGT]^[ACGT]CC
PflMI 190 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 353 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 952 ATTAAT AT^TAAT
SphI 183 GCATGC GCATG^C
SspBI 592 TGTACA T^GTACA
StuI 1094 AGGCCT AGG^CCT
Van91I 190 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 190 CCAGATGCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 411 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 974 CCGC CCGC(-3/-1)
621 GCGG
AluI 617 AGCT AG^CT
888 AGCT
AlwNI 191 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
352 CAGGACCTG
Asp700I 7 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
172 GAAGGAATTC
BclI 708 TGATCA T^GATCA
743 TGATCA
BglI 961 GCCATGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
973 GCCGCCCAGGC
BsgI 397 GTGCAG GTGCAG(16/14)
1139 GTGCAG
BsiYI 190 CCAGATGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1166 CCAATGCAGGG
BslI 190 CCAGATGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1166 CCAATGCAGGG
CfoI 588 GCGC GCG^C
718 GCGC
DraII 353 AGGACCT [GA]G^G[ACGT]CC[CT]
679 AGGGCCT
DsaI 86 CCACGG C^C[GA][CT]GG
334 CCATGG
EcoO109I 353 AGGACCT [GA]G^G[ACGT]CC[CT]
679 AGGGCCT
EcoT14I 334 CCATGG C^C[AT][AT]GG
1065 CCAAGG
FbaI 708 TGATCA T^GATCA
743 TGATCA
HhaI 588 GCGC GCG^C
718 GCGC
HinP1I 588 GCGC G^CGC
718 GCGC
SfcI 126 CTACAG C^T[GA][CT]AG
988 CTACAG
SmlI 415 CTTAAG C^T[CT][GA]AG
1206 CTCAAG
StyI 334 CCATGG C^C[AT][AT]GG
1065 CCAAGG
Tsp45I 486 GTCAC ^GT[GC]AC
518 GTGAC
XmnI 7 GAATATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
172 GAAGGAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI BbsI BbvI BpuAI BsaJI
BsmAI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboI MboII MnlI MseI
MvaI MwoI NdeII NlaIII NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI Tru9I TseI Tsp509I TspRI