Restriction map for
pFN21AB3512
(Linear) (Six-base) MAPSORT of: KIBB3512.seq Check: 256 from: 1 to: 1242
KIBB3512.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AflII C'TTAA_G
Cuts at: 0 415 1242
Size: 415 827
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 746 778 1242
Size: 746 32 464
Fragments arranged by size:
746 464 32
AlwNI CAG_nnn'CTG
Cuts at: 0 196 357 1242
Size: 196 161 885
Fragments arranged by size:
885 196 161
ApoI r'AATT_y
Cuts at: 0 176 1242
Size: 176 1066
AseI AT'TA_AT
Cuts at: 0 953 1242
Size: 953 289
BanII G_rGCy'C
Cuts at: 0 44 1242
Size: 44 1198
BbsI GAAGACnn'nnnn_
Cuts at: 0 68 651 1217 1242
Size: 68 583 566 25
Fragments arranged by size:
583 566 68 25
BclI T'GATC_A
Cuts at: 0 708 743 1242
Size: 708 35 499
Fragments arranged by size:
708 499 35
BglI GCCn_nnn'nGGC
Cuts at: 0 967 979 1242
Size: 967 12 263
Fragments arranged by size:
967 263 12
BglII A'GATC_T
Cuts at: 0 411 1242
Size: 411 831
BmrI ACTGGGnnnn_n'
Cuts at: 0 93 1242
Size: 93 1149
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 648 1242
Size: 648 594
Bpu10I CC'TnA_GC
Cuts at: 0 785 1242
Size: 785 457
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1191 1242
Size: 1191 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 334 1242
Size: 334 908
BsaBI GATnn'nnATC
Cuts at: 0 1161 1242
Size: 1161 81
BseYI C'CCAG_C
Cuts at: 0 200 1232 1242
Size: 200 1032 10
Fragments arranged by size:
1032 200 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1160 1242
Size: 418 742 82
Fragments arranged by size:
742 418 82
Bsp1286I G_dGCh'C
Cuts at: 0 44 1242
Size: 44 1198
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 390 1242
Size: 390 852
BsrGI T'GTAC_A
Cuts at: 0 592 1242
Size: 592 650
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1168 1242
Size: 1168 74
BstEII G'GTnAC_C
Cuts at: 0 18 1242
Size: 18 1224
BstYI r'GATC_y
Cuts at: 0 411 1242
Size: 411 831
BtgI C'CryG_G
Cuts at: 0 86 334 1242
Size: 86 248 908
Fragments arranged by size:
908 248 86
BtsI GCAGTG_nn'
Cuts at: 0 284 1188 1242
Size: 284 904 54
Fragments arranged by size:
904 284 54
EcoO109I rG'GnC_Cy
Cuts at: 0 354 680 1242
Size: 354 326 562
Fragments arranged by size:
562 354 326
EcoRI G'AATT_C
Cuts at: 0 176 1242
Size: 176 1066
FspI TGC'GCA
Cuts at: 0 719 1242
Size: 719 523
HaeII r_GCGC'y
Cuts at: 0 591 1242
Size: 591 651
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 256 288 1207 1239 1242
Size: 256 32 919 32 3
Fragments arranged by size:
919 256 32 32 3
HincII GTy'rAC
Cuts at: 0 225 1242
Size: 225 1017
MfeI C'AATT_G
Cuts at: 0 1145 1242
Size: 1145 97
NcoI C'CATG_G
Cuts at: 0 334 1242
Size: 334 908
NdeI CA'TA_TG
Cuts at: 0 497 1242
Size: 497 745
NspI r_CATG'y
Cuts at: 0 187 1017 1077 1242
Size: 187 830 60 165
Fragments arranged by size:
830 187 165 60
PflMI CCAn_nnn'nTGG
Cuts at: 0 196 1242
Size: 196 1046
PpuMI rG'GwC_Cy
Cuts at: 0 354 1242
Size: 354 888
SfcI C'TryA_G
Cuts at: 0 126 988 1242
Size: 126 862 254
Fragments arranged by size:
862 254 126
SmlI C'TyrA_G
Cuts at: 0 415 1206 1242
Size: 415 791 36
Fragments arranged by size:
791 415 36
SphI G_CATG'C
Cuts at: 0 187 1242
Size: 187 1055
StuI AGG'CCT
Cuts at: 0 1096 1242
Size: 1096 146
StyI C'CwwG_G
Cuts at: 0 334 1065 1242
Size: 334 731 177
Fragments arranged by size:
731 334 177
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 910 1242
Size: 910 332
TatI w'GTAC_w
Cuts at: 0 592 1242
Size: 592 650
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 197 229 843 875 1242
Size: 197 32 614 32 367
Fragments arranged by size:
614 367 197 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 197 1242
Size: 197 1045
XmnI GAAnn'nnTTC
Cuts at: 0 11 176 1242
Size: 11 165 1066
Fragments arranged by size:
1066 165 11
Enzymes that do cut:
AflII AloI AlwNI ApoI AseI BanII BbsI BclI
BglI BglII BmrI BplI Bpu10I BpuEI BsaI BsaBI
BseYI BsgI Bsp1286I BspMI BsrGI BstAPI BstEII BstYI
BtgI BtsI EcoO109I EcoRI FspI HaeII Hin4I HincII
MfeI NcoI NdeI NspI PflMI PpuMI SfcI SmlI
SphI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AlfI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BfrBI BlpI Bme1580I BmgBI BmtI BpmI BsaAI
BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoRV FalI FseI FspAI HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI