Restriction map for
pFN21AB3492
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsiEI BsiHKAI BsiWI
BsoBI Bsp1407I BspDI BspHI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
FauI FbaI FseI FspI HaeII
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MslI MunI NaeI NarI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AccI 434 GTATAC GT^[AC][GT]AC
AccII 41 CGCG CG^CG
AccIII 144 TCCGGA T^CCGGA
AfaI 607 GTAC GT^AC
AflIII 174 ACATGT A^C[GA][CT]GT
AlwI 16 GATCC GGATC(4/5)
BalI 585 TGGCCA TGG^CCA
BanI 67 GGTGCC G^G[CT][GA]CC
BanII 99 GGGCTC G[GA]GC[CT]^C
BfaI 626 CTAG C^TAG
BglI 133 GCCACTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 99 GGGCTC G[AGT]GC[ACT]^C
BsaWI 144 TCCGGA [AT]^CCGG[AT]
BseAI 144 TCCGGA T^CCGGA
BseRI 374 CTCCTC GAGGAG(10/8)
BsgI 227 GTGCAG GTGCAG(16/14)
BsmBI 30 CGTCTC CGTCTC(1/5)
Bsp1286I 99 GGGCTC G[AGT]GC[ACT]^C
BspEI 144 TCCGGA T^CCGGA
BspLU11I 174 ACATGT A^CATGT
BspMI 922 GCAGGT ACCTGC(4/8)
BsrDI 1171 GCAATG GCAATG(2/0)
Bst1107I 434 GTATAC GTA^TAC
BstF5I 1160 GGATG GGATG(2/0)
BstUI 41 CGCG CG^CG
BstYI 15 AGATCC [GA]^GATC[CT]
BstZ17I 434 GTATAC GTA^TAC
CfoI 40 GCGC GCG^C
DraII 92 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 669 CCATGG C^C[GA][CT]GG
EaeI 585 TGGCCA [CT]^GGCC[GA]
EcoO109I 92 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoT22I 458 ATGCAT ATGCA^T
FokI 1160 GGATG GGATG(9/13)
HapII 145 CCGG C^CGG
HhaI 40 GCGC GCG^C
HinP1I 40 GCGC G^CGC
HpaII 145 CCGG C^CGG
MaeI 626 CTAG C^TAG
MaeII 813 ACGT A^CGT
MflI 15 AGATCC [GA]^GATC[CT]
MluNI 585 TGGCCA TGG^CCA
MroI 144 TCCGGA T^CCGGA
MscI 585 TGGCCA TGG^CCA
MspI 145 CCGG C^CGG
MvnI 41 CGCG CG^CG
NcoI 669 CCATGG C^CATGG
NdeI 475 CATATG CA^TATG
NsiI 458 ATGCAT ATGCA^T
NspI 174 ACATGT [GA]CATG^[CT]
PleI 328 GACTC GAGTC(4/5)
PpuMI 92 AGGACCT [GA]G^G[AT]CC[CT]
PstI 773 CTGCAG CTGCA^G
RsaI 607 GTAC GT^AC
SmlI 390 CTTGAG C^T[CT][GA]AG
SspI 1027 AATATT AAT^ATT
StuI 282 AGGCCT AGG^CCT
TaqI 1080 TCGA T^CGA
TfiI 201 GAATC G^A[AT]TC
TthHB8I 1080 TCGA T^CGA
XcmI 264 CCAGATGATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 15 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 37 GCGG CCGC(-3/-1)
42 GCGG
AcsI 233 GAATTC [GA]^AATT[CT]
1082 GAATTC
ApoI 233 GAATTC [GA]^AATT[CT]
1082 GAATTC
BpmI 330 CTCCAG CTGGAG(16/14)
1108 CTGGAG
BsmFI 552 GTCCC GGGAC(10/14)
849 GTCCC
BsmI 3 GAATGC GAATGC(1/-1)
570 GAATGC
BstXI 332 CCAGCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1056 CCACCCTCTTGG
EarI 126 GAAGAG CTCTTC(1/4)
629 GAAGAG
EcoRI 233 GAATTC G^AATTC
1082 GAATTC
EcoT14I 669 CCATGG C^C[AT][AT]GG
1049 CCTTGG
HincII 621 GTCAAC GT[CT]^[GA]AC
943 GTTGAC
HindII 621 GTCAAC GT[CT]^[GA]AC
943 GTTGAC
HinfI 201 GAATC G^A[ACGT]TC
328 GACTC
HphI 412 GGTGA GGTGA(8/7)
737 TCACC
Ksp632I 126 GAAGAG CTCTTC(1/4)
629 GAAGAG
PflMI 90 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
503 CCAAAAAATGG
SfcI 773 CTGCAG C^T[GA][CT]AG
1043 CTATAG
StyI 669 CCATGG C^C[AT][AT]GG
1049 CCTTGG
Van91I 90 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
503 CCAAAAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwNI AvaII BbvI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI HaeIII ItaI MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PvuII Sau3AI Sau96I ScrFI SfaNI
Tru9I TseI Tsp45I Tsp509I TspRI