(Circular) (Six-base) MAPSORT of: pFN21AB3492.seq Check: 1828 from: 1 to: 5896 pFN21AB3492 5896 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3706 278 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2440 3239 1937 Size: 503 799 4594 Fragments arranged by size: 4594 799 503 Acc65I G'GTAC_C Cuts at: 3217 3217 Size: 5896 AclI AA'CG_TT Cuts at: 1563 4023 4396 1563 Size: 2460 373 3063 Fragments arranged by size: 3063 2460 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2742 3959 5343 5611 1632 Size: 1110 1217 1384 268 1917 Fragments arranged by size: 1917 1384 1217 1110 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5699 AflIII A'CryG_T Cuts at: 1203 2179 4795 5772 1203 Size: 976 2616 977 1327 Fragments arranged by size: 2616 1327 977 976 AgeI A'CCGG_T Cuts at: 4727 4727 Size: 5896 AhdI GACnn_n'nnGTC Cuts at: 4625 4625 Size: 5896 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5896 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2281 3166 1184 Size: 1097 885 3914 Fragments arranged by size: 3914 1097 885 AlwNI CAG_nnn'CTG Cuts at: 2024 2101 2810 3639 5211 2024 Size: 77 709 829 1572 2709 Fragments arranged by size: 2709 1572 829 709 77 ApaLI G'TGCA_C Cuts at: 3953 5109 3953 Size: 1156 4740 ApoI r'AATT_y Cuts at: 1493 2238 3087 3205 3431 3583 5692 1493 Size: 745 849 118 226 152 2109 1697 Fragments arranged by size: 2109 1697 849 745 226 152 118 AseI AT'TA_AT Cuts at: 160 1032 4450 160 Size: 872 3418 1606 Fragments arranged by size: 3418 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 AvaI C'yCGr_G Cuts at: 1870 1943 3221 1870 Size: 73 1278 4545 Fragments arranged by size: 4545 1278 73 BamHI G'GATC_C Cuts at: 3226 3593 3226 Size: 367 5529 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 3217 4672 618 Size: 325 217 627 285 1145 1455 1842 Fragments arranged by size: 1842 1455 1145 627 325 285 217 BanII G_rGCy'C Cuts at: 1391 2108 3215 1391 Size: 717 1107 4072 Fragments arranged by size: 4072 1107 717 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4925 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4087 4121 1903 Size: 34 2150 34 3678 Fragments arranged by size: 3678 2150 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3788 4998 3788 Size: 1210 4686 BclI T'GATC_A Cuts at: 1548 1548 Size: 5896 BfrBI ATG'CAT Cuts at: 2465 2465 Size: 5896 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2144 4507 136 Size: 107 122 71 631 605 472 2363 1525 Fragments arranged by size: 2363 1525 631 605 472 122 107 71 BglII A'GATC_T Cuts at: 5891 5891 Size: 5896 BlpI GC'TnA_GC Cuts at: 3313 3313 Size: 5896 Bme1580I G_kGCm'C Cuts at: 1790 3957 5113 1790 Size: 2167 1156 2573 Fragments arranged by size: 2573 2167 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5896 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3627 4585 442 Size: 416 53 83 399 24 2210 958 1753 Fragments arranged by size: 2210 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5896 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2394 2394 Size: 5896 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2320 3134 4556 1239 Size: 1081 814 1422 2579 Fragments arranged by size: 2579 1422 1081 814 Bpu10I CC'TnA_GC Cuts at: 2077 2077 Size: 5896 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2416 4021 4886 5184 2416 Size: 1605 865 298 3128 Fragments arranged by size: 3128 1605 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4559 882 Size: 617 3060 2219 Fragments arranged by size: 3060 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3687 493 Size: 711 2483 2702 Fragments arranged by size: 2702 2483 711 BsaBI GATnn'nnATC Cuts at: 5890 5890 Size: 5896 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3703 4085 4720 275 Size: 53 83 186 936 2170 382 635 1451 Fragments arranged by size: 2170 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2149 4329 4727 5001 5148 5559 2149 Size: 2180 398 274 147 411 2486 Fragments arranged by size: 2486 2180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2090 2120 2131 2161 1178 Size: 30 165 30 687 30 11 30 4913 Fragments arranged by size: 4913 687 165 30 30 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2370 1186 Size: 1184 4712 BseYI C'CCAG_C Cuts at: 2746 5099 5595 2746 Size: 2353 496 3047 Fragments arranged by size: 3047 2353 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2253 1163 Size: 573 517 4806 Fragments arranged by size: 4806 573 517 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3346 4107 5135 1540 Size: 275 186 1345 761 1028 2301 Fragments arranged by size: 2301 1345 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3957 4042 5113 3957 Size: 85 1071 4740 Fragments arranged by size: 4740 1071 85 BsmI GAATG_Cn' Cuts at: 2014 2581 3407 3500 2014 Size: 567 826 93 4410 Fragments arranged by size: 4410 826 567 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2041 2041 Size: 5896 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 3215 3957 4042 5113 1391 Size: 399 318 1107 742 85 1071 2174 Fragments arranged by size: 2174 1107 1071 742 399 318 85 BspEI T'CCGG_A Cuts at: 2149 5559 2149 Size: 3410 2486 BspHI T'CATG_A Cuts at: 3785 5515 3785 Size: 1730 4166 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2918 3251 844 Size: 2074 333 3489 Fragments arranged by size: 3489 2074 333 BsrBI CCG'CTC Cuts at: 1394 1823 3783 1394 Size: 429 1960 3507 Fragments arranged by size: 3507 1960 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3183 4391 4565 65 Size: 1183 1935 1208 174 1396 Fragments arranged by size: 1935 1396 1208 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4540 4727 1700 Size: 110 2730 187 2869 Fragments arranged by size: 2869 2730 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5896 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5896 BssSI C'ACGA_G Cuts at: 3956 4968 3956 Size: 1012 4884 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5896 BstBI TT'CG_AA Cuts at: 4700 4700 Size: 5896 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5896 BstXI CCAn_nnnn'nTGG Cuts at: 2344 3068 2344 Size: 724 5172 BstYI r'GATC_y Cuts at: 1972 2020 3226 3593 3978 3995 5447 5533 5545 Size: 48 1206 367 385 17 1452 86 12 Cuts at: 5545 5891 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1206 385 367 346 86 48 17 12 BstZ17I GTA'TAC Cuts at: 2441 2441 Size: 5896 BtgI C'CryG_G Cuts at: 513 1065 2674 513 Size: 552 1609 3735 Fragments arranged by size: 3735 1609 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5157 Fragments arranged by size: 5157 683 56 BtsI GCAGTG_nn' Cuts at: 3412 4206 4226 3412 Size: 794 20 5082 Fragments arranged by size: 5082 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5896 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5861 DraI TTT'AAA Cuts at: 3200 3200 Size: 5896 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4903 809 Size: 507 3587 1802 Fragments arranged by size: 3587 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2590 3343 4232 Size: 54 1425 50 275 18 760 753 889 Cuts at: 4232 5861 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 760 753 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3343 1537 Size: 275 1531 4090 Fragments arranged by size: 4090 1531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2126 2629 3826 1353 Size: 773 503 1197 3423 Fragments arranged by size: 3423 1197 773 503 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4483 4857 5003 387 Size: 1064 138 2894 374 146 1280 Fragments arranged by size: 2894 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2320 2742 3134 3959 4556 5343 5611 Size: 393 688 422 392 825 597 787 268 Cuts at: 5611 1239 Size: 1524 Fragments arranged by size: 1524 825 787 688 597 422 393 392 268 EcoO109I rG'GnC_Cy Cuts at: 2098 2098 Size: 5896 EcoRI G'AATT_C Cuts at: 2238 3087 3205 3583 2238 Size: 849 118 378 4551 Fragments arranged by size: 4551 849 378 118 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2731 2731 Size: 5896 FspI TGC'GCA Cuts at: 4402 4402 Size: 5896 HaeII r_GCGC'y Cuts at: 1678 5043 1678 Size: 3365 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 4537 4569 4611 Size: 32 135 32 518 32 2415 32 42 Cuts at: 4611 4643 1373 Size: 32 2626 Fragments arranged by size: 2626 2415 518 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2628 2950 3240 3486 669 Size: 988 281 690 322 290 246 3079 Fragments arranged by size: 3079 988 690 322 290 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5896 HpaI GTT'AAC Cuts at: 3486 3486 Size: 5896 KpnI G_GTAC'C Cuts at: 3221 3221 Size: 5896 MfeI C'AATT_G Cuts at: 3495 3495 Size: 5896 MluI A'CGCG_T Cuts at: 5772 5772 Size: 5896 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5010 5194 1305 Size: 3705 184 2007 Fragments arranged by size: 3705 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2592 10 Size: 54 1425 343 760 3314 Fragments arranged by size: 3314 1425 760 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3854 4213 4372 518 Size: 648 2688 359 159 2042 Fragments arranged by size: 2688 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2495 2783 2981 3312 3989 5137 5382 1606 Size: 889 288 198 331 677 1148 245 2120 Fragments arranged by size: 2120 1148 889 677 331 288 245 198 NcoI C'CATG_G Cuts at: 513 1065 2674 513 Size: 552 1609 3735 Fragments arranged by size: 3735 1609 552 NdeI CA'TA_TG Cuts at: 387 2481 387 Size: 2094 3802 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5896 NotI GC'GGCC_GC Cuts at: 3343 3343 Size: 5896 NsiI A_TGCA'T Cuts at: 2467 2467 Size: 5896 NspI r_CATG'y Cuts at: 1132 1229 2183 3254 4799 1132 Size: 97 954 1071 1545 2229 Fragments arranged by size: 2229 1545 1071 954 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5896 PciI A'CATG_T Cuts at: 2179 4795 2179 Size: 2616 3280 PflMI CCAn_nnn'nTGG Cuts at: 1108 2101 2514 1108 Size: 993 413 4490 Fragments arranged by size: 4490 993 413 PfoI T'CCnGG_A Cuts at: 2094 2094 Size: 5896 PmeI GTTT'AAAC Cuts at: 3200 3200 Size: 5896 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3965 3997 5478 5510 3965 Size: 32 1481 32 4351 Fragments arranged by size: 4351 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 2098 Size: 5896 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5896 PsiI TTA'TAA Cuts at: 3466 3466 Size: 5896 PstI C_TGCA'G Cuts at: 830 2782 3248 830 Size: 1952 466 3478 Fragments arranged by size: 3478 1952 466 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5896 PvuII CAG'CTG Cuts at: 2495 2783 2981 2495 Size: 288 198 5410 Fragments arranged by size: 5410 288 198 SalI G'TCGA_C Cuts at: 1936 3238 1936 Size: 1302 4594 SbfI CC_TGCA'GG Cuts at: 3248 3248 Size: 5896 ScaI AGT'ACT Cuts at: 4144 5856 4144 Size: 1712 4184 SfcI C'TryA_G Cuts at: 826 1045 2778 3048 3244 4379 5060 5251 826 Size: 219 1733 270 196 1135 681 191 1471 Fragments arranged by size: 1733 1471 1135 681 270 219 196 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5896 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5896 SmaI CCC'GGG Cuts at: 1872 3223 1872 Size: 1351 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2395 4000 4901 5163 820 Size: 197 926 452 1605 901 262 1553 Fragments arranged by size: 1605 1553 926 901 452 262 197 SnaBI TAC'GTA Cuts at: 493 3687 493 Size: 3194 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5896 SphI G_CATG'C Cuts at: 1132 3254 1132 Size: 2122 3774 SspI AAT'ATT Cuts at: 5 52 3034 3820 5 Size: 47 2982 786 2081 Fragments arranged by size: 2982 2081 786 47 StuI AGG'CCT Cuts at: 1517 2289 1517 Size: 772 5124 StyI C'CwwG_G Cuts at: 513 1065 2674 3054 3335 513 Size: 552 1609 380 281 3074 Fragments arranged by size: 3074 1609 552 380 281 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2219 2852 3923 3940 4093 4278 4723 Size: 1255 639 633 1071 17 153 185 445 Cuts at: 4723 325 Size: 1498 Fragments arranged by size: 1498 1255 1071 639 633 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4142 5854 96 Size: 274 80 33 51 1443 2165 1712 138 Fragments arranged by size: 2165 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2131 2163 5856 5888 2131 Size: 32 3693 32 2139 Fragments arranged by size: 3693 2139 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5896 XbaI T'CTAG_A Cuts at: 3232 3232 Size: 5896 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2276 2276 Size: 5896 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5896 XmaI C'CCGG_G Cuts at: 1870 3221 1870 Size: 1351 4545 XmnI GAAnn'nnTTC Cuts at: 4025 4025 Size: 5896 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3704 276 Size: 53 83 186 936 2170 2468 Fragments arranged by size: 2468 2170 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI