(Circular) (Six-base) MAPSORT of: pFN21AB3465.seq Check: 5387 from: 1 to: 5740 pFN21AB3465 5740 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3550 278 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3083 1937 Size: 1146 4594 Acc65I G'GTAC_C Cuts at: 3061 3061 Size: 5740 AclI AA'CG_TT Cuts at: 1563 3867 4240 1563 Size: 2304 373 3063 Fragments arranged by size: 3063 2304 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 2661 3803 5187 5455 1632 Size: 638 391 1142 1384 268 1917 Fragments arranged by size: 1917 1384 1142 638 391 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5543 AflIII A'CryG_T Cuts at: 1203 4639 5616 1203 Size: 3436 977 1327 Fragments arranged by size: 3436 1327 977 AgeI A'CCGG_T Cuts at: 4571 4571 Size: 5740 AhdI GACnn_n'nnGTC Cuts at: 4469 4469 Size: 5740 AleI CACnn'nnGTG Cuts at: 1166 2787 1166 Size: 1621 4119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5740 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2657 2689 2657 Size: 32 5708 AlwNI CAG_nnn'CTG Cuts at: 2027 3483 5055 2027 Size: 1456 1572 2712 Fragments arranged by size: 2712 1572 1456 ApaLI G'TGCA_C Cuts at: 2893 3797 4953 2893 Size: 904 1156 3680 Fragments arranged by size: 3680 1156 904 ApoI r'AATT_y Cuts at: 1493 2233 2527 3049 3275 3427 5536 1493 Size: 740 294 522 226 152 2109 1697 Fragments arranged by size: 2109 1697 740 522 294 226 152 AseI AT'TA_AT Cuts at: 160 1032 4294 160 Size: 872 3262 1606 Fragments arranged by size: 3262 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 AvaI C'yCGr_G Cuts at: 1870 1943 2419 3065 1870 Size: 73 476 646 4545 Fragments arranged by size: 4545 646 476 73 BamHI G'GATC_C Cuts at: 2424 3070 3437 2424 Size: 646 367 4727 Fragments arranged by size: 4727 646 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3061 4516 618 Size: 325 217 627 1274 1455 1842 Fragments arranged by size: 1842 1455 1274 627 325 217 BanII G_rGCy'C Cuts at: 1391 2420 3059 1391 Size: 1029 639 4072 Fragments arranged by size: 4072 1029 639 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4769 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3931 3965 1903 Size: 34 50 34 1910 34 3678 Fragments arranged by size: 3678 1910 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2752 2772 3632 4842 2752 Size: 20 860 1210 3650 Fragments arranged by size: 3650 1210 860 20 BclI T'GATC_A Cuts at: 1548 1548 Size: 5740 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4351 136 Size: 107 122 71 631 605 2679 1525 Fragments arranged by size: 2679 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5735 5735 Size: 5740 BlpI GC'TnA_GC Cuts at: 3157 3157 Size: 5740 Bme1580I G_kGCm'C Cuts at: 1790 2897 2972 3801 4957 1790 Size: 1107 75 829 1156 2573 Fragments arranged by size: 2573 1156 1107 829 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5740 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2015 3471 4429 Size: 416 53 83 399 24 598 1456 958 Cuts at: 4429 442 Size: 1753 Fragments arranged by size: 1753 1456 958 598 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5740 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2322 2471 4400 1239 Size: 1083 149 1929 2579 Fragments arranged by size: 2579 1929 1083 149 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3865 4730 5028 3865 Size: 865 298 4577 Fragments arranged by size: 4577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2509 2728 4403 882 Size: 617 1010 219 1675 2219 Fragments arranged by size: 2219 1675 1010 617 219 BsaAI yAC'GTr Cuts at: 493 1204 3531 493 Size: 711 2327 2702 Fragments arranged by size: 2702 2327 711 BsaBI GATnn'nnATC Cuts at: 2634 5734 2634 Size: 3100 2640 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3547 3929 4564 275 Size: 53 83 186 936 2014 382 635 1451 Fragments arranged by size: 2014 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2268 4173 4571 4845 4992 5403 2268 Size: 1905 398 274 147 411 2605 Fragments arranged by size: 2605 1905 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5515 Fragments arranged by size: 5515 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2233 2531 1186 Size: 1047 298 4395 Fragments arranged by size: 4395 1047 298 BseYI C'CCAG_C Cuts at: 2228 4943 5439 2228 Size: 2715 496 2529 Fragments arranged by size: 2715 2529 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2366 2474 2741 1163 Size: 573 630 108 267 4162 Fragments arranged by size: 4162 630 573 267 108 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3190 3951 4979 1540 Size: 275 186 1189 761 1028 2301 Fragments arranged by size: 2301 1189 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2897 3801 3886 4957 2897 Size: 904 85 1071 3680 Fragments arranged by size: 3680 1071 904 85 BsmI GAATG_Cn' Cuts at: 3251 3344 3251 Size: 93 5647 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2420 2897 2972 3059 3801 3886 4957 Size: 399 630 477 75 87 742 85 1071 Cuts at: 4957 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 630 477 399 87 85 75 BspEI T'CCGG_A Cuts at: 5403 5403 Size: 5740 BspHI T'CATG_A Cuts at: 3629 5359 3629 Size: 1730 4010 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3095 844 Size: 2251 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3627 1394 Size: 429 1804 3507 Fragments arranged by size: 3507 1804 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2126 4235 4409 65 Size: 1183 878 2109 174 1396 Fragments arranged by size: 2109 1396 1183 878 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2443 4384 4571 1700 Size: 110 633 1941 187 2869 Fragments arranged by size: 2869 1941 633 187 110 BsrGI T'GTAC_A Cuts at: 96 2472 2814 2879 96 Size: 2376 342 65 2957 Fragments arranged by size: 2957 2376 342 65 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5740 BssSI C'ACGA_G Cuts at: 3800 4812 3800 Size: 1012 4728 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5740 BstBI TT'CG_AA Cuts at: 4544 4544 Size: 5740 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5740 BstYI r'GATC_y Cuts at: 1972 2424 3070 3437 3822 3839 5291 5377 5389 Size: 452 646 367 385 17 1452 86 12 Cuts at: 5389 5735 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 646 452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2043 513 Size: 552 978 4210 Fragments arranged by size: 4210 978 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5001 Fragments arranged by size: 5001 683 56 BtsI GCAGTG_nn' Cuts at: 3256 4050 4070 3256 Size: 794 20 4926 Fragments arranged by size: 4926 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5740 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5705 DraI TTT'AAA Cuts at: 3044 3044 Size: 5740 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4747 809 Size: 507 3431 1802 Fragments arranged by size: 3431 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3187 4076 5705 Size: 54 1425 50 275 18 1357 889 1629 Cuts at: 5705 8 Size: 43 Fragments arranged by size: 1629 1425 1357 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3187 1537 Size: 275 1375 4090 Fragments arranged by size: 4090 1375 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2726 3670 1353 Size: 1373 944 3423 Fragments arranged by size: 3423 1373 944 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4327 4701 4847 387 Size: 1064 138 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2322 2471 2661 3803 4400 5187 Size: 393 638 52 149 190 1142 597 787 Cuts at: 5187 5455 1239 Size: 268 1524 Fragments arranged by size: 1524 1142 787 638 597 393 268 190 149 52 EcoNI CCTnn'n_nnAGG Cuts at: 2337 2337 Size: 5740 EcoRI G'AATT_C Cuts at: 2233 3049 3427 2233 Size: 816 378 4546 Fragments arranged by size: 4546 816 378 FspI TGC'GCA Cuts at: 4246 4246 Size: 5740 HaeII r_GCGC'y Cuts at: 1678 4887 1678 Size: 3209 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 4381 4413 4455 Size: 32 135 32 467 32 2310 32 42 Cuts at: 4455 4487 1373 Size: 32 2626 Fragments arranged by size: 2626 2310 467 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3084 3330 669 Size: 988 281 1146 246 3079 Fragments arranged by size: 3079 1146 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5740 HpaI GTT'AAC Cuts at: 3330 3330 Size: 5740 KpnI G_GTAC'C Cuts at: 3065 3065 Size: 5740 MfeI C'AATT_G Cuts at: 3339 3339 Size: 5740 MluI A'CGCG_T Cuts at: 5616 5616 Size: 5740 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4854 5038 1305 Size: 3549 184 2007 Fragments arranged by size: 3549 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3918 Fragments arranged by size: 3918 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2787 3698 4057 4216 518 Size: 648 843 778 911 359 159 2042 Fragments arranged by size: 2042 911 843 778 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2365 2390 3156 3833 4981 5226 1606 Size: 759 25 766 677 1148 245 2120 Fragments arranged by size: 2120 1148 766 759 677 245 25 NcoI C'CATG_G Cuts at: 513 1065 2043 513 Size: 552 978 4210 Fragments arranged by size: 4210 978 552 NdeI CA'TA_TG Cuts at: 387 2566 387 Size: 2179 3561 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5740 NotI GC'GGCC_GC Cuts at: 3187 3187 Size: 5740 NspI r_CATG'y Cuts at: 1132 1229 2296 2545 3034 3098 4643 1132 Size: 97 1067 249 489 64 1545 2229 Fragments arranged by size: 2229 1545 1067 489 249 97 64 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5740 PciI A'CATG_T Cuts at: 4639 4639 Size: 5740 PflMI CCAn_nnn'nTGG Cuts at: 1108 2446 1108 Size: 1338 4402 PmeI GTTT'AAAC Cuts at: 3044 3044 Size: 5740 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3809 3841 5322 5354 3809 Size: 32 1481 32 4195 Fragments arranged by size: 4195 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5740 PsiI TTA'TAA Cuts at: 3310 3310 Size: 5740 PstI C_TGCA'G Cuts at: 830 3092 830 Size: 2262 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5740 PvuII CAG'CTG Cuts at: 2390 2390 Size: 5740 SalI G'TCGA_C Cuts at: 1936 3082 1936 Size: 1146 4594 SbfI CC_TGCA'GG Cuts at: 3092 3092 Size: 5740 ScaI AGT'ACT Cuts at: 3988 5700 3988 Size: 1712 4028 SfcI C'TryA_G Cuts at: 826 1045 3088 4223 4904 5095 826 Size: 219 2043 1135 681 191 1471 Fragments arranged by size: 2043 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5740 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5740 SmaI CCC'GGG Cuts at: 1872 2421 3067 1872 Size: 549 646 4545 Fragments arranged by size: 4545 646 549 SmlI C'TyrA_G Cuts at: 820 1017 1943 3844 4745 5007 820 Size: 197 926 1901 901 262 1553 Fragments arranged by size: 1901 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3531 493 Size: 3038 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5740 SphI G_CATG'C Cuts at: 1132 2296 2545 3098 1132 Size: 1164 249 553 3774 Fragments arranged by size: 3774 1164 553 249 SspI AAT'ATT Cuts at: 5 52 3664 5 Size: 47 3612 2081 Fragments arranged by size: 3612 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5740 StyI C'CwwG_G Cuts at: 513 1065 2043 3179 513 Size: 552 978 1136 3074 Fragments arranged by size: 3074 1136 978 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2701 2901 3767 3784 3937 4122 4567 Size: 1255 1121 200 866 17 153 185 445 Cuts at: 4567 325 Size: 1498 Fragments arranged by size: 1498 1255 1121 866 445 200 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2472 2814 2879 Size: 274 80 33 51 1443 495 342 65 Cuts at: 2879 3986 5698 96 Size: 1107 1712 138 Fragments arranged by size: 1712 1443 1107 495 342 274 138 80 65 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5700 5732 5700 Size: 32 5708 Tth111I GACn'n_nGTC Cuts at: 1316 2051 1316 Size: 735 5005 XbaI T'CTAG_A Cuts at: 3076 3076 Size: 5740 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5740 XmaI C'CCGG_G Cuts at: 1870 2419 3065 1870 Size: 549 646 4545 Fragments arranged by size: 4545 646 549 XmnI GAAnn'nnTTC Cuts at: 3869 3869 Size: 5740 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3548 276 Size: 53 83 186 936 2014 2468 Fragments arranged by size: 2468 2014 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI