(Circular) (Six-base) MAPSORT of: pFN21AB3410.seq Check: 5226 from: 1 to: 6994 pFN21AB3410 6994 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2050 2617 2050 Size: 567 6427 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4804 278 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4337 1937 Size: 2400 4594 Acc65I G'GTAC_C Cuts at: 4315 4315 Size: 6994 AclI AA'CG_TT Cuts at: 1563 5121 5494 1563 Size: 3558 373 3063 Fragments arranged by size: 3558 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2414 2510 3050 3235 3287 5057 6441 Size: 641 141 96 540 185 52 1770 1384 Cuts at: 6441 6709 1632 Size: 268 1917 Fragments arranged by size: 1917 1770 1384 641 540 268 185 141 96 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6797 AflIII A'CryG_T Cuts at: 1203 3258 4119 5893 6870 1203 Size: 2055 861 1774 977 1327 Fragments arranged by size: 2055 1774 1327 977 861 AgeI A'CCGG_T Cuts at: 5825 5825 Size: 6994 AhdI GACnn_n'nnGTC Cuts at: 2413 5723 2413 Size: 3310 3684 AleI CACnn'nnGTG Cuts at: 1166 3965 1166 Size: 2799 4195 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6994 AlwNI CAG_nnn'CTG Cuts at: 2032 2065 2185 2206 2359 2668 2722 2785 2911 Size: 33 120 21 153 309 54 63 126 Cuts at: 2911 3028 3520 4049 4737 6309 2032 Size: 117 492 529 688 1572 2717 Fragments arranged by size: 2717 1572 688 529 492 309 153 126 120 117 63 54 33 21 ApaI G_GGCC'C Cuts at: 2805 2805 Size: 6994 ApaLI G'TGCA_C Cuts at: 3089 5051 6207 3089 Size: 1962 1156 3876 Fragments arranged by size: 3876 1962 1156 ApoI r'AATT_y Cuts at: 1493 3452 4163 4303 4529 4681 6790 1493 Size: 1959 711 140 226 152 2109 1697 Fragments arranged by size: 2109 1959 1697 711 226 152 140 AseI AT'TA_AT Cuts at: 160 1032 5548 160 Size: 872 4516 1606 Fragments arranged by size: 4516 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 AvaI C'yCGr_G Cuts at: 1870 1943 2807 4319 1870 Size: 73 864 1512 4545 Fragments arranged by size: 4545 1512 864 73 AvrII C'CTAG_G Cuts at: 3694 3694 Size: 6994 BamHI G'GATC_C Cuts at: 2016 2397 2547 4324 4691 2016 Size: 381 150 1777 367 4319 Fragments arranged by size: 4319 1777 381 367 150 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3233 3391 4315 5770 618 Size: 325 217 627 1446 158 924 1455 1842 Fragments arranged by size: 1842 1455 1446 924 627 325 217 158 BanII G_rGCy'C Cuts at: 1391 2170 2585 2805 4313 1391 Size: 779 415 220 1508 4072 Fragments arranged by size: 4072 1508 779 415 220 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2714 3038 3937 928 Size: 971 815 324 899 3985 Fragments arranged by size: 3985 971 899 815 324 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3778 3812 5185 5219 1903 Size: 34 1841 34 1373 34 3678 Fragments arranged by size: 3678 1841 1373 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4886 6096 4886 Size: 1210 5784 BclI T'GATC_A Cuts at: 1548 1548 Size: 6994 BfrBI ATG'CAT Cuts at: 4037 4037 Size: 6994 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2570 5605 136 Size: 107 122 71 631 605 898 3035 1525 Fragments arranged by size: 3035 1525 898 631 605 122 107 71 BglII A'GATC_T Cuts at: 6989 6989 Size: 6994 BlpI GC'TnA_GC Cuts at: 2579 2892 4411 2579 Size: 313 1519 5162 Fragments arranged by size: 5162 1519 313 Bme1580I G_kGCm'C Cuts at: 1790 2271 2805 3093 3236 3247 4103 5055 6211 Size: 481 534 288 143 11 856 952 1156 Cuts at: 6211 1790 Size: 2573 Fragments arranged by size: 2573 1156 952 856 534 481 288 143 11 BmgBI CAC'GTC Cuts at: 1318 2824 1318 Size: 1506 5488 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2104 2921 3574 Size: 416 53 83 399 24 687 817 653 Cuts at: 3574 4725 5683 442 Size: 1151 958 1753 Fragments arranged by size: 1753 1151 958 817 687 653 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6994 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3964 4304 3964 Size: 340 6654 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2270 2333 2600 2657 3566 5654 1239 Size: 968 63 63 267 57 909 2088 2579 Fragments arranged by size: 2579 2088 968 909 267 63 63 57 Bpu10I CC'TnA_GC Cuts at: 3899 3899 Size: 6994 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5119 5984 6282 5119 Size: 865 298 5831 Fragments arranged by size: 5831 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4172 5657 882 Size: 617 2673 1485 2219 Fragments arranged by size: 2673 2219 1485 617 BsaAI yAC'GTr Cuts at: 493 1204 4068 4201 4785 493 Size: 711 2864 133 584 2702 Fragments arranged by size: 2864 2702 711 584 133 BsaBI GATnn'nnATC Cuts at: 6988 6988 Size: 6994 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2409 4801 5183 5818 Size: 53 83 186 936 876 2392 382 635 Cuts at: 5818 275 Size: 1451 Fragments arranged by size: 2392 1451 936 876 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5427 5825 6099 6246 6657 5427 Size: 398 274 147 411 5764 Fragments arranged by size: 5764 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2630 2660 1178 Size: 30 165 30 1227 30 5512 Fragments arranged by size: 5512 1227 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 2948 3009 3329 1186 Size: 1279 483 61 320 4851 Fragments arranged by size: 4851 1279 483 320 61 BseYI C'CCAG_C Cuts at: 2025 2178 2263 2393 2565 2574 3889 4049 4090 Size: 153 85 130 172 9 1315 160 41 Cuts at: 4090 6197 6693 2025 Size: 2107 496 2326 Fragments arranged by size: 2326 2107 1315 496 172 160 153 130 85 41 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 3199 3318 3488 1163 Size: 573 483 980 119 170 4669 Fragments arranged by size: 4669 980 573 483 170 119 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4444 5205 6233 1540 Size: 275 186 2443 761 1028 2301 Fragments arranged by size: 2443 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 2898 3093 5055 5140 6211 2226 Size: 672 195 1962 85 1071 3009 Fragments arranged by size: 3009 1962 1071 672 195 85 BsiWI C'GTAC_G Cuts at: 3058 3058 Size: 6994 BsmI GAATG_Cn' Cuts at: 4039 4505 4598 4039 Size: 466 93 6435 Fragments arranged by size: 6435 466 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2034 2600 2034 Size: 566 6428 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2170 2226 2271 2585 2805 2898 3093 Size: 399 380 56 45 314 220 93 195 Cuts at: 3093 3236 3247 4103 4313 5055 5140 6211 1391 Size: 143 11 856 210 742 85 1071 2174 Fragments arranged by size: 2174 1071 856 742 399 380 314 220 210 195 143 93 85 56 45 11 BspEI T'CCGG_A Cuts at: 6657 6657 Size: 6994 BspHI T'CATG_A Cuts at: 4883 6613 4883 Size: 1730 5264 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2050 2617 3735 4349 844 Size: 1206 567 1118 614 3489 Fragments arranged by size: 3489 1206 1118 614 567 BsrBI CCG'CTC Cuts at: 1394 1823 4881 1394 Size: 429 3058 3507 Fragments arranged by size: 3507 3058 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3367 5489 5663 65 Size: 1183 2119 2122 174 1396 Fragments arranged by size: 2122 2119 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2871 3288 3885 4259 5638 5825 1700 Size: 110 1061 417 597 374 1379 187 2869 Fragments arranged by size: 2869 1379 1061 597 417 374 187 110 BsrGI T'GTAC_A Cuts at: 96 2343 96 Size: 2247 4747 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6994 BssSI C'ACGA_G Cuts at: 3500 5054 6066 3500 Size: 1554 1012 4428 Fragments arranged by size: 4428 1554 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 2722 3177 794 Size: 1412 516 455 4611 Fragments arranged by size: 4611 1412 516 455 BstBI TT'CG_AA Cuts at: 5798 5798 Size: 6994 BstEII G'GTnAC_C Cuts at: 1184 2986 1184 Size: 1802 5192 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2746 4072 4189 4201 2146 Size: 600 1326 117 12 4939 Fragments arranged by size: 4939 1326 600 117 12 BstYI r'GATC_y Cuts at: 1972 2016 2397 2547 2880 3189 4324 4691 5076 Size: 44 381 150 333 309 1135 367 385 Cuts at: 5076 5093 6545 6631 6643 6989 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1135 385 381 367 346 333 309 150 86 44 17 12 Bsu36I CC'TnA_GG Cuts at: 2543 3021 2543 Size: 478 6516 BtgI C'CryG_G Cuts at: 513 1065 2004 2129 3228 4182 513 Size: 552 939 125 1099 954 3325 Fragments arranged by size: 3325 1099 954 939 552 125 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3781 487 Size: 683 56 2555 3700 Fragments arranged by size: 3700 2555 683 56 BtsI GCAGTG_nn' Cuts at: 2666 3187 3632 4510 5304 5324 2666 Size: 521 445 878 794 20 4336 Fragments arranged by size: 4336 878 794 521 445 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6994 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6959 DraI TTT'AAA Cuts at: 4298 4298 Size: 6994 DraIII CAC_nnn'GTG Cuts at: 2230 2230 Size: 6994 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6001 809 Size: 507 4685 1802 Fragments arranged by size: 4685 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 2691 2787 Size: 54 1425 50 275 18 241 620 96 Cuts at: 2787 2850 2943 3422 4191 4441 5330 6959 8 Size: 63 93 479 769 250 889 1629 43 Fragments arranged by size: 1629 1425 889 769 620 479 275 250 241 96 93 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4441 1537 Size: 275 2629 4090 Fragments arranged by size: 4090 2629 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3263 4924 1353 Size: 1910 1661 3423 Fragments arranged by size: 3423 1910 1661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2234 5581 5955 6101 387 Size: 1064 138 645 3347 374 146 1280 Fragments arranged by size: 3347 1280 1064 645 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2270 2273 2333 2414 2510 2600 Size: 393 575 63 3 60 81 96 90 Cuts at: 2600 2657 3050 3235 3287 3566 5057 5654 6441 Size: 57 393 185 52 279 1491 597 787 Cuts at: 6441 6709 1239 Size: 268 1524 Fragments arranged by size: 1524 1491 787 597 575 393 393 279 268 185 96 90 81 63 60 57 52 3 EcoNI CCTnn'n_nnAGG Cuts at: 2198 3488 2198 Size: 1290 5704 EcoO109I rG'GnC_Cy Cuts at: 2191 2802 3025 3481 3750 3873 3895 4081 2191 Size: 611 223 456 269 123 22 186 5104 Fragments arranged by size: 5104 611 456 269 223 186 123 22 EcoRI G'AATT_C Cuts at: 4163 4303 4681 4163 Size: 140 378 6476 Fragments arranged by size: 6476 378 140 FspI TGC'GCA Cuts at: 5500 5500 Size: 6994 HaeII r_GCGC'y Cuts at: 1678 3443 4065 6141 1678 Size: 1765 622 2076 2531 Fragments arranged by size: 2531 2076 1765 622 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 2539 2571 2960 Size: 32 135 32 827 32 108 32 389 Cuts at: 2960 2992 5635 5667 5709 5741 1373 Size: 32 2643 32 42 32 2626 Fragments arranged by size: 2643 2626 827 389 135 108 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2407 2953 4338 4584 669 Size: 988 281 469 546 1385 246 3079 Fragments arranged by size: 3079 1385 988 546 469 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6994 HpaI GTT'AAC Cuts at: 4584 4584 Size: 6994 KpnI G_GTAC'C Cuts at: 4319 4319 Size: 6994 MfeI C'AATT_G Cuts at: 2377 4593 2377 Size: 2216 4778 MluI A'CGCG_T Cuts at: 6870 6870 Size: 6994 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6108 6292 1305 Size: 4803 184 2007 Fragments arranged by size: 4803 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4193 10 Size: 54 1425 343 2361 2811 Fragments arranged by size: 2811 2361 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3678 3914 3965 4070 4187 4952 5311 Size: 648 2512 236 51 105 117 765 359 Cuts at: 5311 5470 518 Size: 159 2042 Fragments arranged by size: 2512 2042 765 648 359 236 159 117 105 51 MspA1I CmG'CkG Cuts at: 1606 2032 2304 2325 2569 2578 2719 2785 2911 Size: 426 272 21 244 9 141 66 126 Cuts at: 2911 3430 4094 4410 5087 6235 6480 1606 Size: 519 664 316 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 519 426 316 272 245 244 141 126 66 21 9 NcoI C'CATG_G Cuts at: 513 1065 2004 2129 4182 513 Size: 552 939 125 2053 3325 Fragments arranged by size: 3325 2053 939 552 125 NdeI CA'TA_TG Cuts at: 387 2296 387 Size: 1909 5085 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6994 NotI GC'GGCC_GC Cuts at: 4441 4441 Size: 6994 NsiI A_TGCA'T Cuts at: 4039 4039 Size: 6994 NspI r_CATG'y Cuts at: 1132 1229 3262 4123 4352 5897 1132 Size: 97 2033 861 229 1545 2229 Fragments arranged by size: 2229 2033 1545 861 229 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6994 PciI A'CATG_T Cuts at: 3258 4119 5893 3258 Size: 861 1774 4359 Fragments arranged by size: 4359 1774 861 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 3801 1108 Size: 1077 1616 4301 Fragments arranged by size: 4301 1616 1077 PfoI T'CCnGG_A Cuts at: 2184 2745 2184 Size: 561 6433 PmeI GTTT'AAAC Cuts at: 4298 4298 Size: 6994 PmlI CAC'GTG Cuts at: 4201 4201 Size: 6994 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3305 3329 3337 3356 3361 3388 5063 5095 6576 Size: 24 8 19 5 27 1675 32 1481 Cuts at: 6576 6608 3305 Size: 32 3691 Fragments arranged by size: 3691 1675 1481 32 32 27 24 19 8 5 PpuMI rG'GwC_Cy Cuts at: 3025 3750 3873 3895 3025 Size: 725 123 22 6124 Fragments arranged by size: 6124 725 123 22 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6994 PsiI TTA'TAA Cuts at: 4564 4564 Size: 6994 PspOMI G'GGCC_C Cuts at: 2801 2801 Size: 6994 PstI C_TGCA'G Cuts at: 830 2037 2211 2502 2673 2718 4045 4346 830 Size: 1207 174 291 171 45 1327 301 3478 Fragments arranged by size: 3478 1327 1207 301 291 174 171 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6994 PvuII CAG'CTG Cuts at: 2032 2569 2578 2719 2785 2911 3430 4094 2032 Size: 537 9 141 66 126 519 664 4932 Fragments arranged by size: 4932 664 537 519 141 126 66 9 SalI G'TCGA_C Cuts at: 1936 4336 1936 Size: 2400 4594 SbfI CC_TGCA'GG Cuts at: 4346 4346 Size: 6994 ScaI AGT'ACT Cuts at: 2516 3956 5242 6954 2516 Size: 1440 1286 1712 2556 Fragments arranged by size: 2556 1712 1440 1286 SexAI A'CCwGG_T Cuts at: 2226 2226 Size: 6994 SfcI C'TryA_G Cuts at: 826 1045 2033 2207 2498 2669 2714 2828 3553 Size: 219 988 174 291 171 45 114 725 Cuts at: 3553 4041 4342 5477 6158 6349 826 Size: 488 301 1135 681 191 1471 Fragments arranged by size: 1471 1135 988 725 681 488 301 291 219 191 174 171 114 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6994 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6994 SmaI CCC'GGG Cuts at: 1872 4321 1872 Size: 2449 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5098 5999 6261 820 Size: 197 926 3155 901 262 1553 Fragments arranged by size: 3155 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4785 493 Size: 4292 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6994 SphI G_CATG'C Cuts at: 1132 4352 1132 Size: 3220 3774 SspI AAT'ATT Cuts at: 5 52 4918 5 Size: 47 4866 2081 Fragments arranged by size: 4866 2081 47 StuI AGG'CCT Cuts at: 1517 2642 1517 Size: 1125 5869 StyI C'CwwG_G Cuts at: 513 1065 2004 2129 3489 3694 3933 4182 4433 Size: 552 939 125 1360 205 239 249 251 Cuts at: 4433 513 Size: 3074 Fragments arranged by size: 3074 1360 939 552 251 249 239 205 125 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2192 5021 5038 5191 5376 5821 325 Size: 1255 612 2829 17 153 185 445 1498 Fragments arranged by size: 2829 1498 1255 612 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 2514 3954 Size: 274 80 33 51 1443 366 171 1440 Cuts at: 3954 4144 5240 6952 96 Size: 190 1096 1712 138 Fragments arranged by size: 1712 1443 1440 1096 366 274 190 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3265 3297 6954 6986 3265 Size: 32 3657 32 3273 Fragments arranged by size: 3657 3273 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6994 XbaI T'CTAG_A Cuts at: 4330 4330 Size: 6994 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2182 3680 3874 2182 Size: 1498 194 5302 Fragments arranged by size: 5302 1498 194 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6994 XmaI C'CCGG_G Cuts at: 1870 4319 1870 Size: 2449 4545 XmnI GAAnn'nnTTC Cuts at: 3521 3845 5123 3521 Size: 324 1278 5392 Fragments arranged by size: 5392 1278 324 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4802 276 Size: 53 83 186 936 3268 2468 Fragments arranged by size: 3268 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BaeI BbeI BbvCI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI