Restriction map for pFN21AB3348
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaLI       AscI        Asp718I     AspEI       AspI        
AviII       AvrII       BanI        BbeI        BbsI        
BbvCI       BclI        BfrI        BglII       BlnI        
BpuAI       BseAI       BsiEI       BsiWI       BsmI        
BspDI       BspEI       BspHI       BspLU11I    BsrFI       
BssSI       BstBI       BstEII      BstPI       Bsu36I      
Cfr10I      ClaI        CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HpaI        KasI        
KpnI        MfeI        MluI        MroI        MunI        
NaeI        NarI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PinAI       PmeI        
PshAI       Psp1406I    PvuI        RcaI        RsrII       
SalI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SnaBI       SphI        SspI        SwaI        
Tth111I     XbaI        XcmI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        1414  GACGTC               GACGT^C
AccI         1279  GTATAC               GT^[AC][GT]AC
AcyI         1414  GACGTC               G[GA]^CG[CT]C
AseI          501  ATTAAT               AT^TAAT
AsnI          501  ATTAAT               AT^TAAT
AvaI         1853  CCCGGG               C^[CT]CG[GA]G
BamHI         671  GGATCC               G^GATCC
BbrPI         260  CACGTG               CAC^GTG
BciVI         765  GGATAC               GTATCC(6/5)
BglI          626  GCCGAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI         260  CACGTG               [CT]AC^GT[GA]
BsaBI         528  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI        1414  GACGTC               G[GA]^CG[CT]C
BsaWI        1669  TCCGGT               [AT]^CCGG[AT]
BsmBI        1416  CGTCTC               CGTCTC(1/5)
BsoBI        1853  CCCGGG               C^[CT]CG[GA]G
Bsp1407I      926  TGTACA               T^GTACA
BsrBI         202  CCGCTC               CCGCTC(-3/-3)
BsrGI         926  TGTACA               T^GTACA
BssHII       2001  GCGCGC               G^CGCGC
Bst1107I     1279  GTATAC               GTA^TAC
BstXI         106  CCAGGACTATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1279  GTATAC               GTA^TAC
DraI          795  TTTAAA               TTT^AAA
DrdI         1870  GACCCGCGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI        1887  CCTGGATGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI        1944  GAATTC               G^AATTC
EcoT14I       292  CCTTGG               C^C[AT][AT]GG
HaeII        1497  GGCGCT               [GA]GCGC^[CT]
HgaI           46  GACGC                GACGC(5/10)
Hin1I        1414  GACGTC               G[GA]^CG[CT]C
HindIII      2044  AAGCTT               A^AGCTT
KspI         1873  CCGCGG               CCGC^GG
MamI          528  GATTGACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI          896  ACATGC               [GA]CATG^[CT]
PmaCI         260  CACGTG               CAC^GTG
PmlI          260  CACGTG               CAC^GTG
PshBI         501  ATTAAT               AT^TAAT
SacII        1873  CCGCGG               CCGC^GG
SapI         1524  GAAGAGC              GCTCTTC(1/4)
SbfI         1932  CCTGCAGG             CCTGCA^GG
SexAI         389  ACCAGGT              A^CC[AT]GGT
SmaI         1853  CCCGGG               CCC^GGG
SpeI         1900  ACTAGT               A^CTAGT
Sse8387I     1932  CCTGCAGG             CCTGCA^GG
SspBI         926  TGTACA               T^GTACA
StuI         1447  AGGCCT               AGG^CCT
StyI          292  CCTTGG               C^C[AT][AT]GG
XmaCI        1853  CCCGGG               C^CCGGG
XmaI         1853  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI         1405  AAATTC               [GA]^AATT[CT]
             1944  GAATTC              
ApaI           72  GGGCCC               GGGCC^C
             1856  GGGCCC              
ApoI         1405  AAATTC               [GA]^AATT[CT]
             1944  GAATTC              
Asp700I       946  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1323  GAAGGTTTTC          
BcgI          628  CGAGGAGGCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1017  GCAGACCGTTCG        
BfaI         1901  CTAG                 C^TAG
             1909  CTAG                
DsaI         1873  CCGCGG               C^C[GA][CT]GG
             1950  CCGTGG              
EarI          592  CTCTTC               CTCTTC(1/4)
             1524  GAAGAG              
Ksp632I       592  CTCTTC               CTCTTC(1/4)
             1524  GAAGAG              
MaeI         1901  CTAG                 C^TAG
             1909  CTAG                
MseI          502  TTAA                 T^TAA
              796  TTAA                
MslI          771  CACCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1750  CACGCAGATG          
PflMI        1767  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2010  CCAGATCCTGG         
PleI          872  GACTC                GAGTC(4/5)
              278  GAGTC               
SacI          759  GAGCTC               GAGCT^C
             1207  GAGCTC              
SmlI           95  CTCAAG               C^T[CT][GA]AG
             1456  CTCAAG              
TaqI          657  TCGA                 T^CGA
              870  TCGA                
TfiI          659  GAATC                G^A[AT]TC
             1551  GAATC               
Tru9I         502  TTAA                 T^TAA
              796  TTAA                
Tsp45I        339  GTCAC                ^GT[GC]AC
             1492  GTGAC               
Tsp509I      1406  AATT                 ^AATT
             1945  AATT                
TthHB8I       657  TCGA                 T^CGA
              870  TCGA                
Van91I       1767  CCAGGAAATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2010  CCAGATCCTGG         
XmnI          946  GAAGTGCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1323  GAAGGTTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   85 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AlwNI       AspHI       AvaII       BalI        BanII       
BbvI        BcnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
Bsp1286I    BspMI       BsrDI       BsrI        BssKI       
BstAPI      BstF5I      BstNI       BstUI       BstYI       
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        MaeII       MaeIII      MboI        MboII       
MflI        MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MvnI        MwoI        NciI        
NdeII       NlaIII      NlaIV       PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TseI        TspRI       XhoII       
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