(Circular) (Six-base) MAPSORT of: pFN21AB3338.seq Check: 8631 from: 1 to: 8188 pFN21AB3338 8188 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4415 4415 Size: 8188 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5091 5998 278 Size: 53 83 186 936 3555 907 2468 Fragments arranged by size: 3555 2468 936 907 186 83 53 AccI GT'mk_AC Cuts at: 1937 3660 4767 5295 5531 1937 Size: 1723 1107 528 236 4594 Fragments arranged by size: 4594 1723 1107 528 236 Acc65I G'GTAC_C Cuts at: 5509 5509 Size: 8188 AclI AA'CG_TT Cuts at: 1563 6315 6688 1563 Size: 4752 373 3063 Fragments arranged by size: 4752 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2612 3052 4160 5083 5150 5445 6251 7635 Size: 980 440 1108 923 67 295 806 1384 Cuts at: 7635 7903 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1108 980 923 806 440 295 268 67 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7991 AflIII A'CryG_T Cuts at: 1203 2265 2937 3177 7087 8064 1203 Size: 1062 672 240 3910 977 1327 Fragments arranged by size: 3910 1327 1062 977 672 240 AgeI A'CCGG_T Cuts at: 3912 7019 3912 Size: 3107 5081 AhdI GACnn_n'nnGTC Cuts at: 6917 6917 Size: 8188 AleI CACnn'nnGTG Cuts at: 1166 3314 5219 1166 Size: 2148 1905 4135 Fragments arranged by size: 4135 2148 1905 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8188 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2924 2956 2924 Size: 32 8156 AlwNI CAG_nnn'CTG Cuts at: 2343 2644 2902 3259 3763 4240 4372 4581 5931 Size: 301 258 357 504 477 132 209 1350 Cuts at: 5931 7503 2343 Size: 1572 3028 Fragments arranged by size: 3028 1572 1350 504 477 357 301 258 209 132 ApaI G_GGCC'C Cuts at: 2740 3274 3442 3631 3820 2740 Size: 534 168 189 189 7108 Fragments arranged by size: 7108 534 189 189 168 ApaLI G'TGCA_C Cuts at: 6245 7401 6245 Size: 1156 7032 ApoI r'AATT_y Cuts at: 1493 5497 5723 5875 7984 1493 Size: 4004 226 152 2109 1697 Fragments arranged by size: 4004 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6742 160 Size: 872 5710 1606 Fragments arranged by size: 5710 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8188 AvaI C'yCGr_G Cuts at: 1870 1943 2629 3462 3631 3819 5513 1870 Size: 73 686 833 169 188 1694 4545 Fragments arranged by size: 4545 1694 833 686 188 169 73 AvrII C'CTAG_G Cuts at: 3494 3494 Size: 8188 BamHI G'GATC_C Cuts at: 2650 5518 5885 2650 Size: 2868 367 4953 Fragments arranged by size: 4953 2868 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3134 4622 5509 6964 618 Size: 325 217 627 1347 1488 887 1455 1842 Fragments arranged by size: 1842 1488 1455 1347 887 627 325 217 BanII G_rGCy'C Cuts at: 1391 2390 2740 3164 3274 3442 3631 3820 4181 Size: 999 350 424 110 168 189 189 361 Cuts at: 4181 4221 4265 4388 4398 4501 4616 4749 5263 Size: 40 44 123 10 103 115 133 514 Cuts at: 5263 5338 5507 1391 Size: 75 169 4072 Fragments arranged by size: 4072 999 514 424 361 350 189 189 169 168 133 123 115 110 103 75 44 40 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2452 5292 5433 928 Size: 971 553 2840 141 3683 Fragments arranged by size: 3683 2840 971 553 141 BbvCI CC'TCA_GC Cuts at: 4349 4874 4349 Size: 525 7663 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4458 4492 6379 6413 1903 Size: 34 2521 34 1887 34 3678 Fragments arranged by size: 3678 2521 1887 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3156 6080 7290 3156 Size: 2924 1210 4054 Fragments arranged by size: 4054 2924 1210 BclI T'GATC_A Cuts at: 1548 4776 1548 Size: 3228 4960 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3626 3815 6799 Size: 107 122 71 631 605 1954 189 2984 Cuts at: 6799 136 Size: 1525 Fragments arranged by size: 2984 1954 1525 631 605 189 122 107 71 BglII A'GATC_T Cuts at: 2997 4885 8183 2997 Size: 1888 3298 3002 Fragments arranged by size: 3298 3002 1888 BlpI GC'TnA_GC Cuts at: 2917 4848 5605 2917 Size: 1931 757 5500 Fragments arranged by size: 5500 1931 757 Bme1580I G_kGCm'C Cuts at: 1790 2552 2740 3274 3442 3631 3820 3940 4525 Size: 762 188 534 168 189 189 120 585 Cuts at: 4525 4551 6249 7405 1790 Size: 26 1698 1156 2573 Fragments arranged by size: 2573 1698 1156 762 585 534 189 189 188 168 120 26 BmgBI CAC'GTC Cuts at: 1318 3616 1318 Size: 2298 5890 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3013 3927 4127 Size: 416 53 83 399 24 1596 914 200 Cuts at: 4127 5919 6877 442 Size: 1792 958 1753 Fragments arranged by size: 1792 1753 1596 958 914 416 399 200 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3236 1056 Size: 2180 6008 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5016 5016 Size: 8188 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2183 3150 4412 4763 5432 6848 1239 Size: 944 967 1262 351 669 1416 2579 Fragments arranged by size: 2579 1416 1262 967 944 669 351 Bpu10I CC'TnA_GC Cuts at: 3839 4349 4874 3839 Size: 510 525 7153 Fragments arranged by size: 7153 525 510 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 6313 7178 7476 6313 Size: 865 298 7025 Fragments arranged by size: 7025 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6851 882 Size: 617 5352 2219 Fragments arranged by size: 5352 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2938 5979 493 Size: 711 1734 3041 2702 Fragments arranged by size: 3041 2702 1734 711 BsaBI GATnn'nnATC Cuts at: 2453 8182 2453 Size: 5729 2459 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2421 3954 5088 5995 Size: 53 83 186 936 888 1533 1134 907 Cuts at: 5995 6377 7012 275 Size: 382 635 1451 Fragments arranged by size: 1533 1451 1134 936 907 888 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2619 3912 6621 7019 7293 7440 7851 2619 Size: 1293 2709 398 274 147 411 2956 Fragments arranged by size: 2956 2709 1293 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5078 5108 1178 Size: 30 165 30 3675 30 4258 Fragments arranged by size: 4258 3675 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2087 5126 1186 Size: 901 3039 4248 Fragments arranged by size: 4248 3039 901 BseYI C'CCAG_C Cuts at: 2383 2394 3726 3918 3947 5004 5151 5229 7391 Size: 11 1332 192 29 1057 147 78 2162 Cuts at: 7391 7887 2383 Size: 496 2684 Fragments arranged by size: 2684 2162 1332 1057 496 192 147 78 29 11 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2144 2225 2318 3527 4175 1163 Size: 573 408 81 93 1209 648 5176 Fragments arranged by size: 5176 1209 648 573 408 93 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5638 6399 7427 1540 Size: 275 186 3637 761 1028 2301 Fragments arranged by size: 3637 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2158 3834 4388 4398 4749 5263 6249 6334 7405 Size: 1676 554 10 351 514 986 85 1071 Cuts at: 7405 2158 Size: 2941 Fragments arranged by size: 2941 1676 1071 986 554 514 351 85 10 BsmI GAATG_Cn' Cuts at: 5008 5699 5792 5008 Size: 691 93 7404 Fragments arranged by size: 7404 691 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2390 2552 2740 3164 3274 3442 Size: 399 368 232 162 188 424 110 168 Cuts at: 3442 3631 3820 3834 3940 4181 4221 4265 4388 Size: 189 189 14 106 241 40 44 123 Cuts at: 4388 4398 4501 4525 4551 4616 4749 5263 5338 Size: 10 103 24 26 65 133 514 75 Cuts at: 5338 5507 6249 6334 7405 1391 Size: 169 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 514 424 399 368 241 232 189 189 188 169 168 162 133 123 110 106 103 85 75 65 44 40 26 24 14 10 BspEI T'CCGG_A Cuts at: 2619 7851 2619 Size: 5232 2956 BspHI T'CATG_A Cuts at: 6077 7807 6077 Size: 1730 6458 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4248 4415 5543 844 Size: 3404 167 1128 3489 Fragments arranged by size: 3489 3404 1128 167 BsrBI CCG'CTC Cuts at: 1394 1823 6075 1394 Size: 429 4252 3507 Fragments arranged by size: 4252 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3332 5058 5165 6683 6857 65 Size: 1183 2084 1726 107 1518 174 1396 Fragments arranged by size: 2084 1726 1518 1396 1183 174 107 BsrFI r'CCGG_y Cuts at: 1700 1810 2216 3850 3912 6832 7019 1700 Size: 110 406 1634 62 2920 187 2869 Fragments arranged by size: 2920 2869 1634 406 187 110 62 BsrGI T'GTAC_A Cuts at: 96 5370 96 Size: 5274 2914 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8188 BssSI C'ACGA_G Cuts at: 2144 3047 4059 6248 7260 2144 Size: 903 1012 2189 1012 3072 Fragments arranged by size: 3072 2189 1012 1012 903 BstAPI GCAn_nnn'nTGC Cuts at: 794 4581 794 Size: 3787 4401 BstBI TT'CG_AA Cuts at: 6992 6992 Size: 8188 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8188 BstXI CCAn_nnnn'nTGG Cuts at: 3940 5317 3940 Size: 1377 6811 BstYI r'GATC_y Cuts at: 1972 2135 2595 2650 2757 2997 4885 5518 5885 Size: 163 460 55 107 240 1888 633 367 Cuts at: 5885 6270 6287 7739 7825 7837 8183 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1888 1452 633 460 385 367 346 240 163 107 86 55 17 12 Bsu36I CC'TnA_GG Cuts at: 2253 2253 Size: 8188 BtgI C'CryG_G Cuts at: 513 1065 2688 3393 3532 3810 4257 4377 4446 Size: 552 1623 705 139 278 447 120 69 Cuts at: 4446 513 Size: 4255 Fragments arranged by size: 4255 1623 705 552 447 278 139 120 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3423 487 Size: 683 56 2197 5252 Fragments arranged by size: 5252 2197 683 56 BtsI GCAGTG_nn' Cuts at: 2886 3515 3521 4278 4591 4705 5430 5704 6498 Size: 629 6 757 313 114 725 274 794 Cuts at: 6498 6518 2886 Size: 20 4556 Fragments arranged by size: 4556 794 757 725 629 313 274 114 20 6 ClaI AT'CG_AT Cuts at: 1554 4855 1554 Size: 3301 4887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8153 DraI TTT'AAA Cuts at: 5492 5492 Size: 8188 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3959 4340 7195 809 Size: 507 2643 381 2855 1802 Fragments arranged by size: 2855 2643 1802 507 381 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2175 2685 3618 Size: 54 1425 50 275 18 345 510 933 Cuts at: 3618 3807 3870 3897 4254 4890 5635 6524 8153 Size: 189 63 27 357 636 745 889 1629 Cuts at: 8153 8 Size: 43 Fragments arranged by size: 1629 1425 933 889 745 636 510 357 345 275 189 63 54 50 43 27 18 EagI C'GGCC_G Cuts at: 1537 1812 5635 1537 Size: 275 3823 4090 Fragments arranged by size: 4090 3823 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3101 3918 4280 6118 1353 Size: 1748 817 362 1838 3423 Fragments arranged by size: 3423 1838 1748 817 362 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2237 6775 7149 7295 387 Size: 1064 138 648 4538 374 146 1280 Fragments arranged by size: 4538 1280 1064 648 374 146 138 EcoICRI GAG'CTC Cuts at: 4386 4396 4747 5261 4386 Size: 10 351 514 7313 Fragments arranged by size: 7313 514 351 10 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2183 2612 3052 3150 4160 4412 4763 Size: 393 551 429 440 98 1010 252 351 Cuts at: 4763 5083 5150 5432 5445 6251 6848 7635 7903 Size: 320 67 282 13 806 597 787 268 Cuts at: 7903 1239 Size: 1524 Fragments arranged by size: 1524 1010 806 787 597 551 440 429 393 351 320 282 268 252 98 67 13 EcoNI CCTnn'n_nnAGG Cuts at: 3242 3493 3785 3242 Size: 251 292 7645 Fragments arranged by size: 7645 292 251 EcoO109I rG'GnC_Cy Cuts at: 2736 2882 3270 3271 3378 3438 3439 3627 3628 Size: 146 388 1 107 60 1 188 1 Cuts at: 3628 3778 3816 4333 4564 4960 2736 Size: 150 38 517 231 396 5964 Fragments arranged by size: 5964 517 396 388 231 188 150 146 107 60 38 1 1 1 EcoRI G'AATT_C Cuts at: 5497 5875 5497 Size: 378 7810 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2617 4750 5398 2617 Size: 2133 648 5407 Fragments arranged by size: 5407 2133 648 FspI TGC'GCA Cuts at: 2206 6694 2206 Size: 4488 3700 HaeII r_GCGC'y Cuts at: 1678 2924 3764 5354 7335 1678 Size: 1246 840 1590 1981 2531 Fragments arranged by size: 2531 1981 1590 1246 840 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2127 2159 3757 3789 4206 Size: 32 135 32 555 32 1598 32 417 Cuts at: 4206 4238 4949 4981 5078 5110 6829 6861 6903 Size: 32 711 32 97 32 1719 32 42 Cuts at: 6903 6935 1373 Size: 32 2626 Fragments arranged by size: 2626 1719 1598 711 555 417 135 97 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4591 5532 5778 669 Size: 988 281 2653 941 246 3079 Fragments arranged by size: 3079 2653 988 941 281 246 HindIII A'AGCT_T Cuts at: 748 5375 748 Size: 4627 3561 HpaI GTT'AAC Cuts at: 5778 5778 Size: 8188 KpnI G_GTAC'C Cuts at: 5513 5513 Size: 8188 MfeI C'AATT_G Cuts at: 5787 5787 Size: 8188 MluI A'CGCG_T Cuts at: 8064 8064 Size: 8188 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2686 7302 7486 1305 Size: 1381 4616 184 2007 Fragments arranged by size: 4616 2007 1381 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3620 3872 3899 4892 10 Size: 54 1425 343 1788 252 27 993 3306 Fragments arranged by size: 3306 1788 1425 993 343 252 54 27 MslI CAynn'nnrTG Cuts at: 518 1166 2219 2730 3176 3314 3450 4518 5219 Size: 648 1053 511 446 138 136 1068 701 Cuts at: 5219 5315 6146 6505 6664 518 Size: 96 831 359 159 2042 Fragments arranged by size: 2042 1068 1053 831 701 648 511 446 359 159 138 136 96 MspA1I CmG'CkG Cuts at: 1606 2210 2239 2986 3712 4581 4741 5082 5460 Size: 604 29 747 726 869 160 341 378 Cuts at: 5460 5604 6281 7429 7674 1606 Size: 144 677 1148 245 2120 Fragments arranged by size: 2120 1148 869 747 726 677 604 378 341 245 160 144 29 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7636 NdeI CA'TA_TG Cuts at: 387 387 Size: 8188 NheI G'CTAG_C Cuts at: 1052 3232 1052 Size: 2180 6008 NotI GC'GGCC_GC Cuts at: 5635 5635 Size: 8188 NspI r_CATG'y Cuts at: 1132 1229 2224 2269 3181 4539 5546 7091 1132 Size: 97 995 45 912 1358 1007 1545 2229 Fragments arranged by size: 2229 1545 1358 1007 995 912 97 45 PasI CC'CwG_GG Cuts at: 1348 3552 4111 4663 1348 Size: 2204 559 552 4873 Fragments arranged by size: 4873 2204 559 552 PciI A'CATG_T Cuts at: 2265 3177 7087 2265 Size: 912 3910 3366 Fragments arranged by size: 3910 3366 912 PflMI CCAn_nnn'nTGG Cuts at: 1108 3327 1108 Size: 2219 5969 PfoI T'CCnGG_A Cuts at: 2088 5106 5236 2088 Size: 3018 130 5040 Fragments arranged by size: 5040 3018 130 PmeI GTTT'AAAC Cuts at: 5492 5492 Size: 8188 PmlI CAC'GTG Cuts at: 2938 2938 Size: 8188 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2345 2377 6257 6289 7770 7802 2345 Size: 32 3880 32 1481 32 2731 Fragments arranged by size: 3880 2731 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2882 3778 4333 2882 Size: 896 555 6737 Fragments arranged by size: 6737 896 555 PshAI GACnn'nnGTC Cuts at: 1613 4044 4489 1613 Size: 2431 445 5312 Fragments arranged by size: 5312 2431 445 PsiI TTA'TAA Cuts at: 5758 5758 Size: 8188 PspOMI G'GGCC_C Cuts at: 2736 3270 3438 3627 3816 2736 Size: 534 168 189 189 7108 Fragments arranged by size: 7108 534 189 189 168 PstI C_TGCA'G Cuts at: 830 2991 3747 4368 4586 5076 5540 830 Size: 2161 756 621 218 490 464 3478 Fragments arranged by size: 3478 2161 756 621 490 464 218 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8188 PvuII CAG'CTG Cuts at: 2210 2986 4741 5460 2210 Size: 776 1755 719 4938 Fragments arranged by size: 4938 1755 776 719 SacI G_AGCT'C Cuts at: 4388 4398 4749 5263 4388 Size: 10 351 514 7313 Fragments arranged by size: 7313 514 351 10 SalI G'TCGA_C Cuts at: 1936 5530 1936 Size: 3594 4594 SanDI GG'GwC_CC Cuts at: 2882 2882 Size: 8188 SbfI CC_TGCA'GG Cuts at: 5540 5540 Size: 8188 ScaI AGT'ACT Cuts at: 2579 4805 6436 8148 2579 Size: 2226 1631 1712 2619 Fragments arranged by size: 2619 2226 1712 1631 SexAI A'CCwGG_T Cuts at: 3258 3258 Size: 8188 SfcI C'TryA_G Cuts at: 826 1045 2987 3743 4364 4582 5072 5536 6671 Size: 219 1942 756 621 218 490 464 1135 Cuts at: 6671 7352 7543 826 Size: 681 191 1471 Fragments arranged by size: 1942 1471 1135 756 681 621 490 464 219 218 191 SfiI GGCCn_nnn'nGGCC Cuts at: 3626 3815 3626 Size: 189 7999 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8188 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8188 SmaI CCC'GGG Cuts at: 1872 3633 3821 5515 1872 Size: 1761 188 1694 4545 Fragments arranged by size: 4545 1761 1694 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 6292 7193 7455 820 Size: 197 926 4349 901 262 1553 Fragments arranged by size: 4349 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5979 493 Size: 5486 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8188 SphI G_CATG'C Cuts at: 1132 5546 1132 Size: 4414 3774 SspI AAT'ATT Cuts at: 5 52 6112 5 Size: 47 6060 2081 Fragments arranged by size: 6060 2081 47 StuI AGG'CCT Cuts at: 1517 2673 4362 1517 Size: 1156 1689 5343 Fragments arranged by size: 5343 1689 1156 StyI C'CwwG_G Cuts at: 513 1065 2271 2965 3494 3900 5627 513 Size: 552 1206 694 529 406 1727 3074 Fragments arranged by size: 3074 1727 1206 694 552 529 406 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2627 3326 3800 4328 6215 6232 6385 Size: 1255 1047 699 474 528 1887 17 153 Cuts at: 6385 6570 7015 325 Size: 185 445 1498 Fragments arranged by size: 1887 1498 1255 1047 699 528 474 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2577 2787 4803 Size: 274 80 33 51 1443 600 210 2016 Cuts at: 4803 5223 5370 6434 8146 96 Size: 420 147 1064 1712 138 Fragments arranged by size: 2016 1712 1443 1064 600 420 274 210 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3038 3070 4114 4146 4411 4443 8148 8180 3038 Size: 32 1044 32 265 32 3705 32 3046 Fragments arranged by size: 3705 3046 1044 265 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2456 5291 1316 Size: 1140 2835 4213 Fragments arranged by size: 4213 2835 1140 XbaI T'CTAG_A Cuts at: 5524 5524 Size: 8188 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4738 4738 Size: 8188 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8188 XmaI C'CCGG_G Cuts at: 1870 3631 3819 5513 1870 Size: 1761 188 1694 4545 Fragments arranged by size: 4545 1761 1694 188 XmnI GAAnn'nnTTC Cuts at: 6317 6317 Size: 8188 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5089 5996 276 Size: 53 83 186 936 3555 907 2468 Fragments arranged by size: 3555 2468 936 907 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SapI SfoI SrfI SwaI