Restriction map for
pFN21AB3338
(Linear) (Six-base) MAPSORT of: KIBB3338.seq Check: 2401 from: 1 to: 3483
KIBB3338.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2410 3483
Size: 2410 1073
AatII G_ACGT'C
Cuts at: 0 3086 3483
Size: 3086 397
AccI GT'mk_AC
Cuts at: 0 1655 2762 3290 3483
Size: 1655 1107 528 193
Fragments arranged by size:
1655 1107 528 193
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 607 1047 2155 3078 3145 3440 3483
Size: 607 440 1108 923 67 295 43
Fragments arranged by size:
1108 923 607 440 295 67 43
AflIII A'CryG_T
Cuts at: 0 260 932 1172 3483
Size: 260 672 240 2311
Fragments arranged by size:
2311 672 260 240
AgeI A'CCGG_T
Cuts at: 0 1907 3483
Size: 1907 1576
AleI CACnn'nnGTG
Cuts at: 0 1309 3214 3483
Size: 1309 1905 269
Fragments arranged by size:
1905 1309 269
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 919 951 3483
Size: 919 32 2532
Fragments arranged by size:
2532 919 32
AlwNI CAG_nnn'CTG
Cuts at: 0 338 639 897 1254 1758 2235 2367 2576
Size: 338 301 258 357 504 477 132 209
Cuts at: 2576 3483
Size: 907
Fragments arranged by size:
907 504 477 357 338 301 258 209
132
ApaI G_GGCC'C
Cuts at: 0 735 1269 1437 1626 1815 3483
Size: 735 534 168 189 189 1668
Fragments arranged by size:
1668 735 534 189 189 168
AvaI C'yCGr_G
Cuts at: 0 624 1457 1626 1814 3483
Size: 624 833 169 188 1669
Fragments arranged by size:
1669 833 624 188 169
AvrII C'CTAG_G
Cuts at: 0 1489 3483
Size: 1489 1994
BamHI G'GATC_C
Cuts at: 0 645 3483
Size: 645 2838
BanI G'GyrC_C
Cuts at: 0 1129 2617 3483
Size: 1129 1488 866
Fragments arranged by size:
1488 1129 866
BanII G_rGCy'C
Cuts at: 0 385 735 1159 1269 1437 1626 1815 2176
Size: 385 350 424 110 168 189 189 361
Cuts at: 2176 2216 2260 2383 2393 2496 2611 2744 3258
Size: 40 44 123 10 103 115 133 514
Cuts at: 3258 3333 3483
Size: 75 150
Fragments arranged by size:
514 424 385 361 350 189 189 168
150 133 123 115 110 103 75 44
40 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 447 3287 3428 3483
Size: 447 2840 141 55
Fragments arranged by size:
2840 447 141 55
BbvCI CC'TCA_GC
Cuts at: 0 2344 2869 3483
Size: 2344 525 614
Fragments arranged by size:
2344 614 525
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2453 2487 3483
Size: 2453 34 996
Fragments arranged by size:
2453 996 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1151 3483
Size: 1151 2332
BclI T'GATC_A
Cuts at: 0 2771 3483
Size: 2771 712
BglI GCCn_nnn'nGGC
Cuts at: 0 1621 1810 3483
Size: 1621 189 1673
Fragments arranged by size:
1673 1621 189
BglII A'GATC_T
Cuts at: 0 992 2880 3483
Size: 992 1888 603
Fragments arranged by size:
1888 992 603
BlpI GC'TnA_GC
Cuts at: 0 912 2843 3483
Size: 912 1931 640
Fragments arranged by size:
1931 912 640
Bme1580I G_kGCm'C
Cuts at: 0 547 735 1269 1437 1626 1815 1935 2520
Size: 547 188 534 168 189 189 120 585
Cuts at: 2520 2546 3483
Size: 26 937
Fragments arranged by size:
937 585 547 534 189 189 188 168
120 26
BmgBI CAC'GTC
Cuts at: 0 1611 3483
Size: 1611 1872
BmrI ACTGGGnnnn_n'
Cuts at: 0 1008 1922 2122 3483
Size: 1008 914 200 1361
Fragments arranged by size:
1361 1008 914 200
BmtI G_CTAG'C
Cuts at: 0 1231 3483
Size: 1231 2252
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3011 3483
Size: 3011 472
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 1145 2407 2758 3427 3483
Size: 178 967 1262 351 669 56
Fragments arranged by size:
1262 967 669 351 178 56
Bpu10I CC'TnA_GC
Cuts at: 0 1834 2344 2869 3483
Size: 1834 510 525 614
Fragments arranged by size:
1834 614 525 510
BsaAI yAC'GTr
Cuts at: 0 933 3483
Size: 933 2550
BsaBI GATnn'nnATC
Cuts at: 0 448 3483
Size: 448 3035
BsaHI Gr'CG_yC
Cuts at: 0 416 1949 3083 3483
Size: 416 1533 1134 400
Fragments arranged by size:
1533 1134 416 400
BsaWI w'CCGG_w
Cuts at: 0 614 1907 3483
Size: 614 1293 1576
Fragments arranged by size:
1576 1293 614
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3073 3103 3483
Size: 3073 30 380
Fragments arranged by size:
3073 380 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 82 3121 3483
Size: 82 3039 362
Fragments arranged by size:
3039 362 82
BseYI C'CCAG_C
Cuts at: 0 378 389 1721 1913 1942 2999 3146 3224
Size: 378 11 1332 192 29 1057 147 78
Cuts at: 3224 3483
Size: 259
Fragments arranged by size:
1332 1057 378 259 192 147 78 29
11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 220 313 1522 2170 3483
Size: 139 81 93 1209 648 1313
Fragments arranged by size:
1313 1209 648 139 93 81
BsiHKAI G_wGCw'C
Cuts at: 0 153 1829 2383 2393 2744 3258 3483
Size: 153 1676 554 10 351 514 225
Fragments arranged by size:
1676 554 514 351 225 153 10
BsmI GAATG_Cn'
Cuts at: 0 3003 3483
Size: 3003 480
Bsp1286I G_dGCh'C
Cuts at: 0 153 385 547 735 1159 1269 1437 1626
Size: 153 232 162 188 424 110 168 189
Cuts at: 1626 1815 1829 1935 2176 2216 2260 2383 2393
Size: 189 14 106 241 40 44 123 10
Cuts at: 2393 2496 2520 2546 2611 2744 3258 3333 3483
Size: 103 24 26 65 133 514 75 150
Fragments arranged by size:
514 424 241 232 189 189 188 168
162 153 150 133 123 110 106 103
75 65 44 40 26 24 14 10
BspEI T'CCGG_A
Cuts at: 0 614 3483
Size: 614 2869
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2243 2410 3483
Size: 2243 167 1073
Fragments arranged by size:
2243 1073 167
BsrDI GCAATG_nn'
Cuts at: 0 1327 3053 3160 3483
Size: 1327 1726 107 323
Fragments arranged by size:
1726 1327 323 107
BsrFI r'CCGG_y
Cuts at: 0 211 1845 1907 3483
Size: 211 1634 62 1576
Fragments arranged by size:
1634 1576 211 62
BsrGI T'GTAC_A
Cuts at: 0 3365 3483
Size: 3365 118
BssSI C'ACGA_G
Cuts at: 0 139 1042 2054 3483
Size: 139 903 1012 1429
Fragments arranged by size:
1429 1012 903 139
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2576 3483
Size: 2576 907
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1935 3312 3483
Size: 1935 1377 171
Fragments arranged by size:
1935 1377 171
BstYI r'GATC_y
Cuts at: 0 130 590 645 752 992 2880 3483
Size: 130 460 55 107 240 1888 603
Fragments arranged by size:
1888 603 460 240 130 107 55
Bsu36I CC'TnA_GG
Cuts at: 0 248 3483
Size: 248 3235
BtgI C'CryG_G
Cuts at: 0 683 1388 1527 1805 2252 2372 2441 3483
Size: 683 705 139 278 447 120 69 1042
Fragments arranged by size:
1042 705 683 447 278 139 120 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1418 3483
Size: 1418 2065
BtsI GCAGTG_nn'
Cuts at: 0 881 1510 1516 2273 2586 2700 3425 3483
Size: 881 629 6 757 313 114 725 58
Fragments arranged by size:
881 757 725 629 313 114 58 6
ClaI AT'CG_AT
Cuts at: 0 2850 3483
Size: 2850 633
DrdI GACnn_nn'nnGTC
Cuts at: 0 1954 2335 3483
Size: 1954 381 1148
Fragments arranged by size:
1954 1148 381
EaeI y'GGCC_r
Cuts at: 0 170 680 1613 1802 1865 1892 2249 2885
Size: 170 510 933 189 63 27 357 636
Cuts at: 2885 3483
Size: 598
Fragments arranged by size:
933 636 598 510 357 189 170 63
27
EarI CTCTTCn'nnn_
Cuts at: 0 1096 1913 2275 3483
Size: 1096 817 362 1208
Fragments arranged by size:
1208 1096 817 362
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 232 3483
Size: 232 3251
EcoICRI GAG'CTC
Cuts at: 0 2381 2391 2742 3256 3483
Size: 2381 10 351 514 227
Fragments arranged by size:
2381 514 351 227 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 607 1047 1145 2155 2407 2758 3078
Size: 178 429 440 98 1010 252 351 320
Cuts at: 3078 3145 3427 3440 3483
Size: 67 282 13 43
Fragments arranged by size:
1010 440 429 351 320 282 252 178
98 67 43 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1237 1488 1780 3483
Size: 1237 251 292 1703
Fragments arranged by size:
1703 1237 292 251
EcoO109I rG'GnC_Cy
Cuts at: 0 731 877 1265 1266 1373 1433 1434 1622
Size: 731 146 388 1 107 60 1 188
Cuts at: 1622 1623 1773 1811 2328 2559 2955 3483
Size: 1 150 38 517 231 396 528
Fragments arranged by size:
731 528 517 396 388 231 188 150
146 107 60 38 1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 612 2745 3393 3483
Size: 612 2133 648 90
Fragments arranged by size:
2133 648 612 90
FspI TGC'GCA
Cuts at: 0 201 3483
Size: 201 3282
HaeII r_GCGC'y
Cuts at: 0 919 1759 3349 3483
Size: 919 840 1590 134
Fragments arranged by size:
1590 919 840 134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 122 154 1752 1784 2201 2233 2944 2976
Size: 122 32 1598 32 417 32 711 32
Cuts at: 2976 3073 3105 3483
Size: 97 32 378
Fragments arranged by size:
1598 711 417 378 122 97 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2586 3483
Size: 2586 897
HindIII A'AGCT_T
Cuts at: 0 3370 3483
Size: 3370 113
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 681 3483
Size: 681 2802
MscI TGG'CCA
Cuts at: 0 1615 1867 1894 2887 3483
Size: 1615 252 27 993 596
Fragments arranged by size:
1615 993 596 252 27
MslI CAynn'nnrTG
Cuts at: 0 214 725 1171 1309 1445 2513 3214 3310
Size: 214 511 446 138 136 1068 701 96
Cuts at: 3310 3483
Size: 173
Fragments arranged by size:
1068 701 511 446 214 173 138 136
96
MspA1I CmG'CkG
Cuts at: 0 205 234 981 1707 2576 2736 3077 3455
Size: 205 29 747 726 869 160 341 378
Cuts at: 3455 3483
Size: 28
Fragments arranged by size:
869 747 726 378 341 205 160 29
28
NheI G'CTAG_C
Cuts at: 0 1227 3483
Size: 1227 2256
NspI r_CATG'y
Cuts at: 0 219 264 1176 2534 3483
Size: 219 45 912 1358 949
Fragments arranged by size:
1358 949 912 219 45
PasI CC'CwG_GG
Cuts at: 0 1547 2106 2658 3483
Size: 1547 559 552 825
Fragments arranged by size:
1547 825 559 552
PciI A'CATG_T
Cuts at: 0 260 1172 3483
Size: 260 912 2311
Fragments arranged by size:
2311 912 260
PflMI CCAn_nnn'nTGG
Cuts at: 0 1322 3483
Size: 1322 2161
PfoI T'CCnGG_A
Cuts at: 0 83 3101 3231 3483
Size: 83 3018 130 252
Fragments arranged by size:
3018 252 130 83
PmlI CAC'GTG
Cuts at: 0 933 3483
Size: 933 2550
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 340 372 3483
Size: 340 32 3111
Fragments arranged by size:
3111 340 32
PpuMI rG'GwC_Cy
Cuts at: 0 877 1773 2328 3483
Size: 877 896 555 1155
Fragments arranged by size:
1155 896 877 555
PshAI GACnn'nnGTC
Cuts at: 0 2039 2484 3483
Size: 2039 445 999
Fragments arranged by size:
2039 999 445
PspOMI G'GGCC_C
Cuts at: 0 731 1265 1433 1622 1811 3483
Size: 731 534 168 189 189 1672
Fragments arranged by size:
1672 731 534 189 189 168
PstI C_TGCA'G
Cuts at: 0 986 1742 2363 2581 3071 3483
Size: 986 756 621 218 490 412
Fragments arranged by size:
986 756 621 490 412 218
PvuII CAG'CTG
Cuts at: 0 205 981 2736 3455 3483
Size: 205 776 1755 719 28
Fragments arranged by size:
1755 776 719 205 28
SacI G_AGCT'C
Cuts at: 0 2383 2393 2744 3258 3483
Size: 2383 10 351 514 225
Fragments arranged by size:
2383 514 351 225 10
SanDI GG'GwC_CC
Cuts at: 0 877 3483
Size: 877 2606
ScaI AGT'ACT
Cuts at: 0 574 2800 3483
Size: 574 2226 683
Fragments arranged by size:
2226 683 574
SexAI A'CCwGG_T
Cuts at: 0 1253 3483
Size: 1253 2230
SfcI C'TryA_G
Cuts at: 0 982 1738 2359 2577 3067 3483
Size: 982 756 621 218 490 416
Fragments arranged by size:
982 756 621 490 416 218
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1621 1810 3483
Size: 1621 189 1673
Fragments arranged by size:
1673 1621 189
SmaI CCC'GGG
Cuts at: 0 1628 1816 3483
Size: 1628 188 1667
Fragments arranged by size:
1667 1628 188
StuI AGG'CCT
Cuts at: 0 668 2357 3483
Size: 668 1689 1126
Fragments arranged by size:
1689 1126 668
StyI C'CwwG_G
Cuts at: 0 266 960 1489 1895 3483
Size: 266 694 529 406 1588
Fragments arranged by size:
1588 694 529 406 266
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 622 1321 1795 2323 3483
Size: 622 699 474 528 1160
Fragments arranged by size:
1160 699 622 528 474
TatI w'GTAC_w
Cuts at: 0 572 782 2798 3218 3365 3483
Size: 572 210 2016 420 147 118
Fragments arranged by size:
2016 572 420 210 147 118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1033 1065 2109 2141 2406 2438 3483
Size: 1033 32 1044 32 265 32 1045
Fragments arranged by size:
1045 1044 1033 265 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 451 3286 3483
Size: 451 2835 197
Fragments arranged by size:
2835 451 197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2733 3483
Size: 2733 750
XmaI C'CCGG_G
Cuts at: 0 1626 1814 3483
Size: 1626 188 1669
Fragments arranged by size:
1669 1626 188
ZraI GAC'GTC
Cuts at: 0 3084 3483
Size: 3084 399
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AgeI AleI AloI
AlwNI ApaI AvaI AvrII BamHI BanI BanII BbsI
BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI HaeII Hin4I HincII HindIII MmeI MscI MslI
MspA1I NheI NspI PasI PciI PflMI PfoI PmlI
PpiI PpuMI PshAI PspOMI PstI PvuII SacI SanDI
ScaI SexAI SfcI SfiI SmaI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AhdI AlfI ApaLI ApoI
AscI AseI AsiSI BaeI BbeI BfrBI BpuEI BsaI
BsiEI BsiWI BsmBI BspHI BsrBI BssHII BstBI BstEII
BstZ17I CspCI DraI DraIII EagI EcoRI EcoRV FseI
FspAI HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NdeI NgoMIV NotI NruI NsiI PacI PmeI
PsiI PspXI PsrI PvuI RsrII SacII SalI SapI
SbfI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI