(Circular) (Six-base) MAPSORT of: pFN21AB3319.seq Check: 5430 from: 1 to: 7123 pFN21AB3319 7123 bp With 182 enzymes: SgfI * October 20, 2009 10:33 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2193 3442 3910 2193 Size: 1249 468 5406 Fragments arranged by size: 5406 1249 468 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4933 278 Size: 53 83 186 936 3397 2468 Fragments arranged by size: 3397 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3885 4466 1937 Size: 1948 581 4594 Fragments arranged by size: 4594 1948 581 Acc65I G'GTAC_C Cuts at: 4444 4444 Size: 7123 AclI AA'CG_TT Cuts at: 1563 5250 5623 1563 Size: 3687 373 3063 Fragments arranged by size: 3687 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2408 3125 3749 5186 6570 6838 1632 Size: 776 717 624 1437 1384 268 1917 Fragments arranged by size: 1917 1437 1384 776 717 624 268 AfeI AGC'GCT Cuts at: 3356 3782 4106 3356 Size: 426 324 6373 Fragments arranged by size: 6373 426 324 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6926 AflIII A'CryG_T Cuts at: 1203 2067 2479 3616 6022 6999 1203 Size: 864 412 1137 2406 977 1327 Fragments arranged by size: 2406 1327 1137 977 864 412 AgeI A'CCGG_T Cuts at: 5954 5954 Size: 7123 AhdI GACnn_n'nnGTC Cuts at: 5852 5852 Size: 7123 AleI CACnn'nnGTG Cuts at: 1166 4059 1166 Size: 2893 4230 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7123 AlwNI CAG_nnn'CTG Cuts at: 2135 2383 3553 4325 4866 6438 2135 Size: 248 1170 772 541 1572 2820 Fragments arranged by size: 2820 1572 1170 772 541 248 ApaI G_GGCC'C Cuts at: 2156 3267 3688 4412 2156 Size: 1111 421 724 4867 Fragments arranged by size: 4867 1111 724 421 ApaLI G'TGCA_C Cuts at: 2180 5180 6336 2180 Size: 3000 1156 2967 Fragments arranged by size: 3000 2967 1156 ApoI r'AATT_y Cuts at: 1493 4432 4658 4810 6919 1493 Size: 2939 226 152 2109 1697 Fragments arranged by size: 2939 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5677 160 Size: 872 4645 1606 Fragments arranged by size: 4645 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 AvaI C'yCGr_G Cuts at: 1870 1943 2298 4448 1870 Size: 73 355 2150 4545 Fragments arranged by size: 4545 2150 355 73 BamHI G'GATC_C Cuts at: 3154 4171 4453 4820 3154 Size: 1017 282 367 5457 Fragments arranged by size: 5457 1017 367 282 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2576 2747 3893 4061 4444 Size: 325 217 627 789 171 1146 168 383 Cuts at: 4444 5899 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1146 789 627 383 325 217 171 168 BanII G_rGCy'C Cuts at: 1391 2156 2604 3267 3290 3688 3990 4412 4442 Size: 765 448 663 23 398 302 422 30 Cuts at: 4442 1391 Size: 4072 Fragments arranged by size: 4072 765 663 448 422 398 302 30 23 BbeI G_GCGC'C Cuts at: 3897 3897 Size: 7123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2531 928 Size: 971 632 5520 Fragments arranged by size: 5520 971 632 BbvCI CC'TCA_GC Cuts at: 4182 4182 Size: 7123 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3033 3067 5314 5348 1903 Size: 34 1096 34 2247 34 3678 Fragments arranged by size: 3678 2247 1096 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4255 5015 6225 4255 Size: 760 1210 5153 Fragments arranged by size: 5153 1210 760 BclI T'GATC_A Cuts at: 1548 1548 Size: 7123 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3755 4416 5734 Size: 107 122 71 631 605 2083 661 1318 Cuts at: 5734 136 Size: 1525 Fragments arranged by size: 2083 1525 1318 661 631 605 122 107 71 BglII A'GATC_T Cuts at: 2262 2361 7118 2262 Size: 99 4757 2267 Fragments arranged by size: 4757 2267 99 BlpI GC'TnA_GC Cuts at: 2270 3794 4101 4108 4540 2270 Size: 1524 307 7 432 4853 Fragments arranged by size: 4853 1524 432 307 7 Bme1580I G_kGCm'C Cuts at: 1790 2156 2184 2244 2581 2752 2967 3267 3688 Size: 366 28 60 337 171 215 300 421 Cuts at: 3688 4366 4412 5184 6340 1790 Size: 678 46 772 1156 2573 Fragments arranged by size: 2573 1156 772 678 421 366 337 300 215 171 60 46 28 BmgBI CAC'GTC Cuts at: 1318 2068 1318 Size: 750 6373 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2551 2620 2791 Size: 416 53 83 399 24 1134 69 171 Cuts at: 2791 4854 5812 442 Size: 2063 958 1753 Fragments arranged by size: 2063 1753 1134 958 416 399 171 83 69 53 24 BmtI G_CTAG'C Cuts at: 1056 2868 1056 Size: 1812 5311 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3826 3826 Size: 7123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2161 2405 2576 2654 3950 4079 4177 4265 Size: 922 244 171 78 1296 129 98 88 Cuts at: 4265 4292 4358 5783 1239 Size: 27 66 1425 2579 Fragments arranged by size: 2579 1425 1296 922 244 171 129 98 88 78 66 27 Bpu10I CC'TnA_GC Cuts at: 4182 4229 4182 Size: 47 7076 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2344 5248 6113 6411 2344 Size: 2904 865 298 3056 Fragments arranged by size: 3056 2904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2501 3224 3797 5786 882 Size: 617 1002 723 573 1989 2219 Fragments arranged by size: 2219 1989 1002 723 617 573 BsaAI yAC'GTr Cuts at: 493 1204 2218 2480 3617 3913 4914 493 Size: 711 1014 262 1137 296 1001 2702 Fragments arranged by size: 2702 1137 1014 1001 711 296 262 BsaBI GATnn'nnATC Cuts at: 7117 7117 Size: 7123 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3276 3894 4930 5312 Size: 53 83 186 936 1743 618 1036 382 Cuts at: 5312 5947 275 Size: 635 1451 Fragments arranged by size: 1743 1451 1036 936 635 618 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5556 5954 6228 6375 6786 5556 Size: 398 274 147 411 5893 Fragments arranged by size: 5893 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3125 3155 1178 Size: 30 165 30 1722 30 5146 Fragments arranged by size: 5146 1722 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2158 2183 2738 2867 3146 3230 3365 3755 Size: 972 25 555 129 279 84 135 390 Cuts at: 3755 4385 1186 Size: 630 3924 Fragments arranged by size: 3924 972 630 555 390 279 135 129 84 25 BseYI C'CCAG_C Cuts at: 2960 3553 3676 6326 6822 2960 Size: 593 123 2650 496 3261 Fragments arranged by size: 3261 2650 593 496 123 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2372 2396 2858 3077 3320 3545 3572 Size: 573 636 24 462 219 243 225 27 Cuts at: 3572 3935 1163 Size: 363 4351 Fragments arranged by size: 4351 636 573 462 363 243 225 219 27 24 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4573 5334 6362 1540 Size: 275 186 2572 761 1028 2301 Fragments arranged by size: 2572 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2184 2604 3375 3990 4347 5184 5269 6340 2184 Size: 420 771 615 357 837 85 1071 2967 Fragments arranged by size: 2967 1071 837 771 615 420 357 85 BsmI GAATG_Cn' Cuts at: 4634 4727 4634 Size: 93 7030 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2184 2244 2581 2604 2752 2967 Size: 399 366 28 60 337 23 148 215 Cuts at: 2967 3267 3290 3375 3688 3990 4347 4366 4412 Size: 300 23 85 313 302 357 19 46 Cuts at: 4412 4442 5184 5269 6340 1391 Size: 30 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 357 337 313 302 300 215 148 85 85 60 46 30 28 23 23 19 BspEI T'CCGG_A Cuts at: 6786 6786 Size: 7123 BspHI T'CATG_A Cuts at: 5012 6742 5012 Size: 1730 5393 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2193 3099 3442 3910 4094 4478 844 Size: 1349 906 343 468 184 384 3489 Fragments arranged by size: 3489 1349 906 468 384 343 184 BsrBI CCG'CTC Cuts at: 1394 1823 3498 5010 1394 Size: 429 1675 1512 3507 Fragments arranged by size: 3507 1675 1512 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5618 5792 65 Size: 1183 4370 174 1396 Fragments arranged by size: 4370 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3339 3636 3749 4115 5767 5954 1700 Size: 110 1529 297 113 366 1652 187 2869 Fragments arranged by size: 2869 1652 1529 366 297 187 113 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7123 BssHII G'CGCG_C Cuts at: 1925 2049 4074 4076 1925 Size: 124 2025 2 4972 Fragments arranged by size: 4972 2025 124 2 BssSI C'ACGA_G Cuts at: 3074 3095 4367 5183 6195 3074 Size: 21 1272 816 1012 4002 Fragments arranged by size: 4002 1272 1012 816 21 BstAPI GCAn_nnn'nTGC Cuts at: 794 4325 794 Size: 3531 3592 BstBI TT'CG_AA Cuts at: 5927 5927 Size: 7123 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7123 BstXI CCAn_nnnn'nTGG Cuts at: 2437 2929 3055 2437 Size: 492 126 6505 Fragments arranged by size: 6505 492 126 BstYI r'GATC_y Cuts at: 1972 2262 2361 3154 3189 4171 4453 4820 5205 Size: 290 99 793 35 982 282 367 385 Cuts at: 5205 5222 6674 6760 6772 7118 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 982 793 385 367 346 290 282 99 86 35 17 12 Bsu36I CC'TnA_GG Cuts at: 4296 4296 Size: 7123 BtgI C'CryG_G Cuts at: 513 1065 2004 3006 3291 3873 513 Size: 552 939 1002 285 582 3763 Fragments arranged by size: 3763 1002 939 582 552 285 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6384 Fragments arranged by size: 6384 683 56 BtsI GCAGTG_nn' Cuts at: 2588 2859 3500 3611 4639 5433 5453 2588 Size: 271 641 111 1028 794 20 4258 Fragments arranged by size: 4258 1028 794 641 271 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7123 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3748 3783 529 Size: 35 3184 35 3869 Fragments arranged by size: 3869 3184 35 35 DraI TTT'AAA Cuts at: 4427 4427 Size: 7123 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6130 809 Size: 507 4814 1802 Fragments arranged by size: 4814 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2121 2947 Size: 54 1425 50 275 18 177 114 826 Cuts at: 2947 3396 3702 3756 4044 4570 5459 7088 8 Size: 449 306 54 288 526 889 1629 43 Fragments arranged by size: 1629 1425 889 826 526 449 306 288 275 177 114 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4570 1537 Size: 275 2758 4090 Fragments arranged by size: 4090 2758 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2880 3029 3419 5053 1353 Size: 1527 149 390 1634 3423 Fragments arranged by size: 3423 1634 1527 390 149 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2687 5710 6084 6230 387 Size: 1064 138 1098 3023 374 146 1280 Fragments arranged by size: 3023 1280 1098 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2602 3988 2602 Size: 1386 5737 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2161 2405 2408 2576 2654 3125 3749 Size: 393 529 244 3 168 78 471 624 Cuts at: 3749 3950 4079 4177 4265 4292 4358 5186 5783 Size: 201 129 98 88 27 66 828 597 Cuts at: 5783 6570 6838 1239 Size: 787 268 1524 Fragments arranged by size: 1524 828 787 624 597 529 471 393 268 244 201 168 129 98 88 78 66 27 3 EcoNI CCTnn'n_nnAGG Cuts at: 2774 3095 4337 2774 Size: 321 1242 5560 Fragments arranged by size: 5560 1242 321 EcoO109I rG'GnC_Cy Cuts at: 2153 2434 2914 3100 3263 3684 3685 4287 4300 Size: 281 480 186 163 421 1 602 13 Cuts at: 4300 4409 2153 Size: 109 4867 Fragments arranged by size: 4867 602 480 421 281 186 163 109 13 1 EcoRI G'AATT_C Cuts at: 4432 4810 4432 Size: 378 6745 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2548 2650 2548 Size: 102 7021 FspI TGC'GCA Cuts at: 4013 5629 4013 Size: 1616 5507 HaeII r_GCGC'y Cuts at: 1678 3301 3358 3784 3897 3950 4026 4108 6270 Size: 1623 57 426 113 53 76 82 2162 Cuts at: 6270 1678 Size: 2531 Fragments arranged by size: 2531 2162 1623 426 113 82 76 57 53 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4183 4215 5764 5796 5838 Size: 32 135 32 2611 32 1549 32 42 Cuts at: 5838 5870 1373 Size: 32 2626 Fragments arranged by size: 2626 2611 1549 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4467 4713 669 Size: 988 281 2529 246 3079 Fragments arranged by size: 3079 2529 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7123 HpaI GTT'AAC Cuts at: 4713 4713 Size: 7123 KasI G'GCGC_C Cuts at: 3893 3893 Size: 7123 KpnI G_GTAC'C Cuts at: 4448 4448 Size: 7123 MfeI C'AATT_G Cuts at: 4722 4722 Size: 7123 MluI A'CGCG_T Cuts at: 6999 6999 Size: 7123 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3925 6237 6421 1305 Size: 2620 2312 184 2007 Fragments arranged by size: 2620 2312 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 2123 3398 3758 10 Size: 54 1425 343 177 114 1275 360 3375 Fragments arranged by size: 3375 1425 1275 360 343 177 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2544 3854 4059 5081 5440 5599 518 Size: 648 1378 1310 205 1022 359 159 2042 Fragments arranged by size: 2042 1378 1310 1022 648 359 205 159 MspA1I CmG'CkG Cuts at: 1606 2206 2313 2403 3334 3553 3798 4139 4207 Size: 600 107 90 931 219 245 341 68 Cuts at: 4207 4539 5216 6364 6609 1606 Size: 332 677 1148 245 2120 Fragments arranged by size: 2120 1148 931 677 600 341 332 245 245 219 107 90 68 NaeI GCC'GGC Cuts at: 3751 4117 3751 Size: 366 6757 NarI GG'CG_CC Cuts at: 3894 3894 Size: 7123 NcoI C'CATG_G Cuts at: 513 1065 2004 3006 513 Size: 552 939 1002 4630 Fragments arranged by size: 4630 1002 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7123 NgoMIV G'CCGG_C Cuts at: 3749 4115 3749 Size: 366 6757 NheI G'CTAG_C Cuts at: 1052 2864 1052 Size: 1812 5311 NotI GC'GGCC_GC Cuts at: 4570 4570 Size: 7123 NspI r_CATG'y Cuts at: 1132 1229 3085 4481 6026 1132 Size: 97 1856 1396 1545 2229 Fragments arranged by size: 2229 1856 1545 1396 97 PasI CC'CwG_GG Cuts at: 1348 4147 4405 1348 Size: 2799 258 4066 Fragments arranged by size: 4066 2799 258 PciI A'CATG_T Cuts at: 6022 6022 Size: 7123 PflMI CCAn_nnn'nTGG Cuts at: 1108 2206 2503 3553 1108 Size: 1098 297 1050 4678 Fragments arranged by size: 4678 1098 1050 297 PfoI T'CCnGG_A Cuts at: 3630 3630 Size: 7123 PmeI GTTT'AAAC Cuts at: 4427 4427 Size: 7123 PmlI CAC'GTG Cuts at: 2480 3617 3913 2480 Size: 1137 296 5690 Fragments arranged by size: 5690 1137 296 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5192 5224 6705 6737 5192 Size: 32 1481 32 5578 Fragments arranged by size: 5578 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2434 2914 3100 4300 2434 Size: 480 186 1200 5257 Fragments arranged by size: 5257 1200 480 186 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7123 PsiI TTA'TAA Cuts at: 4693 4693 Size: 7123 PspOMI G'GGCC_C Cuts at: 2152 3263 3684 4408 2152 Size: 1111 421 724 4867 Fragments arranged by size: 4867 1111 724 421 PstI C_TGCA'G Cuts at: 830 3606 4124 4475 830 Size: 2776 518 351 3478 Fragments arranged by size: 3478 2776 518 351 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7123 PvuII CAG'CTG Cuts at: 2206 2313 3334 3553 4139 4207 2206 Size: 107 1021 219 586 68 5122 Fragments arranged by size: 5122 1021 586 219 107 68 SacI G_AGCT'C Cuts at: 2604 3990 2604 Size: 1386 5737 SalI G'TCGA_C Cuts at: 1936 4465 1936 Size: 2529 4594 SapI GCTCTTCn'nnn_ Cuts at: 2880 2880 Size: 7123 SbfI CC_TGCA'GG Cuts at: 4475 4475 Size: 7123 ScaI AGT'ACT Cuts at: 5371 7083 5371 Size: 1712 5411 SfcI C'TryA_G Cuts at: 826 1045 3602 4120 4471 5606 6287 6478 826 Size: 219 2557 518 351 1135 681 191 1471 Fragments arranged by size: 2557 1471 1135 681 518 351 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4416 4416 Size: 7123 SfoI GGC'GCC Cuts at: 3895 3895 Size: 7123 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7123 SgrAI Cr'CCGG_yG Cuts at: 1700 3339 1700 Size: 1639 5484 SmaI CCC'GGG Cuts at: 1872 4450 1872 Size: 2578 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2323 5227 6128 6390 820 Size: 197 926 380 2904 901 262 1553 Fragments arranged by size: 2904 1553 926 901 380 262 197 SnaBI TAC'GTA Cuts at: 493 4914 493 Size: 4421 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7123 SphI G_CATG'C Cuts at: 1132 3085 4481 1132 Size: 1953 1396 3774 Fragments arranged by size: 3774 1953 1396 SspI AAT'ATT Cuts at: 5 52 5047 5 Size: 47 4995 2081 Fragments arranged by size: 4995 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7123 StyI C'CwwG_G Cuts at: 513 1065 2004 2061 2871 2895 3006 3516 3559 Size: 552 939 57 810 24 111 510 43 Cuts at: 3559 4414 4562 513 Size: 855 148 3074 Fragments arranged by size: 3074 939 855 810 552 510 148 111 57 43 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2735 5150 5167 5320 5505 5950 325 Size: 1255 1155 2415 17 153 185 445 1498 Fragments arranged by size: 2415 1498 1255 1155 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2172 3141 5369 Size: 274 80 33 51 1443 195 969 2228 Cuts at: 5369 7081 96 Size: 1712 138 Fragments arranged by size: 2228 1712 1443 969 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2731 2763 3423 3455 7083 7115 2731 Size: 32 660 32 3628 32 2739 Fragments arranged by size: 3628 2739 660 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2234 2783 1316 Size: 918 549 5656 Fragments arranged by size: 5656 918 549 XbaI T'CTAG_A Cuts at: 4459 4459 Size: 7123 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2688 2688 Size: 7123 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7123 XmaI C'CCGG_G Cuts at: 1870 4448 1870 Size: 2578 4545 XmnI GAAnn'nnTTC Cuts at: 2633 5252 2633 Size: 2619 4504 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4931 276 Size: 53 83 186 936 3397 2468 Fragments arranged by size: 3397 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SrfI SwaI