Restriction map for
pFN21AB3316
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AflIII Alw44I AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BsaAI BsaBI BsaHI
BsiEI BsiWI BsmBI BsoBI BspDI
BspLU11I BsrDI BssHII BssSI BstAPI
BstEII BstPI BstUI BstXI Bsu36I
ClaI CpoI DrdI EaeI EagI
EclXI Eco52I Eco81I EcoO65I EcoT22I
FauI FbaI FseI FspI HgaI
Hin1I HindIII KasI KpnI KspI
MamI MluI MluNI MscI MslI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI PacI PaeR7I PflFI PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SfcI SfiI SfoI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 1656 TCCGGA T^CCGGA
AgeI 1834 ACCGGT A^CCGGT
Aor51HI 61 AGCGCT AGC^GCT
AspHI 2014 GTGCTC G[AT]GC[AT]^C
BanI 2098 GGTGCC G^G[CT][GA]CC
BanII 48 GAGCCC G[GA]GC[CT]^C
BbsI 1715 GTCTTC GAAGAC(2/6)
BlpI 2176 GCTCAGC GC^T[ACGT]AGC
BpmI 707 CTCCAG CTGGAG(16/14)
Bpu1102I 2176 GCTCAGC GC^T[ACGT]AGC
BpuAI 1715 GTCTTC GAAGAC(2/6)
BseAI 1656 TCCGGA T^CCGGA
BsgI 935 GTGCAG GTGCAG(16/14)
BsiHKAI 2014 GTGCTC G[AT]GC[AT]^C
BsmFI 2042 GGGAC GGGAC(10/14)
Bsp1407I 1209 TGTACA T^GTACA
BspEI 1656 TCCGGA T^CCGGA
BspHI 1721 TCATGA T^CATGA
BspMI 1035 ACCTGC ACCTGC(4/8)
BsrFI 1834 ACCGGT [GA]^CCGG[CT]
BsrGI 1209 TGTACA T^GTACA
Bst1107I 1313 GTATAC GTA^TAC
BstBI 1667 TTCGAA TT^CGAA
BstYI 189 AGATCC [GA]^GATC[CT]
BstZ17I 1313 GTATAC GTA^TAC
CelII 2176 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1834 ACCGGT [GA]^CCGG[CT]
DraII 1955 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 1010 GAAGAG CTCTTC(1/4)
Eco47III 61 AGCGCT AGC^GCT
EcoNI 799 CCTTTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1955 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1932 GAATTC G^AATTC
EcoRV 358 GATATC GAT^ATC
HaeII 61 AGCGCT [GA]GCGC^[CT]
HincII 624 GTTAAC GT[CT]^[GA]AC
HindII 624 GTTAAC GT[CT]^[GA]AC
HpaI 624 GTTAAC GTT^AAC
Ksp632I 1010 GAAGAG CTCTTC(1/4)
MaeII 354 ACGT A^CGT
MfeI 1217 CAATTG C^AATTG
MflI 189 AGATCC [GA]^GATC[CT]
MroI 1656 TCCGGA T^CCGGA
MunI 1217 CAATTG C^AATTG
NcoI 2102 CCATGG C^CATGG
NspV 1667 TTCGAA TT^CGAA
PinAI 1834 ACCGGT A^CCGGT
PpuMI 1955 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1721 TCATGA T^CATGA
SexAI 1958 ACCTGGT A^CC[AT]GGT
SfuI 1667 TTCGAA TT^CGAA
SspBI 1209 TGTACA T^GTACA
SspI 1881 AATATT AAT^ATT
StuI 1048 AGGCCT AGG^CCT
XhoII 189 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1313 GTATAC GT^[AC][GT]AC
1981 GTAGAC
AfaI 791 GTAC GT^AC
1210 GTAC
AhdI 748 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACCTGGTGTC
AlwNI 266 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1993 CAGAGACTG
AspEI 748 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACCTGGTGTC
BmyI 48 GAGCCC G[AGT]GC[ACT]^C
2014 GTGCTC
BsaI 329 GAGACC GGTCTC(1/5)
1370 GAGACC
BsaWI 1656 TCCGGA [AT]^CCGG[AT]
1834 ACCGGT
BsiYI 799 CCTTTCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1036 CCTGCAACGGG
BslI 799 CCTTTCCAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1036 CCTGCAACGGG
Bsp1286I 48 GAGCCC G[AGT]GC[ACT]^C
2014 GTGCTC
BsrBI 2163 CCGCTC CCGCTC(-3/-3)
1703 GAGCGG
DraIII 1118 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
1913 CACTCAGTG
DsaI 25 CCGTGG C^C[GA][CT]GG
2102 CCATGG
Eam1105I 748 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1957 GACCTGGTGTC
HapII 1657 CCGG C^CGG
1835 CCGG
HpaII 1657 CCGG C^CGG
1835 CCGG
MspI 1657 CCGG C^CGG
1835 CCGG
PvuII 13 CAGCTG CAG^CTG
1696 CAGCTG
RsaI 791 GTAC GT^AC
1210 GTAC
Tsp45I 614 GTCAC ^GT[GC]AC
2139 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
Asp700I AvaII BbvI BfaI BsaJI
BseRI BsmAI BsmI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraI EcoRII EcoT14I
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
PleI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp509I TspRI TthHB8I XmnI