(Circular) (Six-base) MAPSORT of: pFN21AB3316.seq Check: 8086 from: 1 to: 6922 pFN21AB3316 6922 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4732 278 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3319 3987 4265 1937 Size: 1382 668 278 4594 Fragments arranged by size: 4594 1382 668 278 Acc65I G'GTAC_C Cuts at: 4243 4243 Size: 6922 AclI AA'CG_TT Cuts at: 1563 5049 5422 1563 Size: 3486 373 3063 Fragments arranged by size: 3486 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2151 2442 2904 3078 4985 6369 6637 1632 Size: 519 291 462 174 1907 1384 268 1917 Fragments arranged by size: 1917 1907 1384 519 462 291 268 174 AfeI AGC'GCT Cuts at: 2068 2068 Size: 6922 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6725 AflIII A'CryG_T Cuts at: 1203 5821 6798 1203 Size: 4618 977 1327 Fragments arranged by size: 4618 1327 977 AgeI A'CCGG_T Cuts at: 3839 5753 3839 Size: 1914 5008 AhdI GACnn_n'nnGTC Cuts at: 2758 3967 5651 2758 Size: 1209 1684 4029 Fragments arranged by size: 4029 1684 1209 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6922 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2310 1184 Size: 1126 5796 AlwNI CAG_nnn'CTG Cuts at: 2276 4003 4665 6237 2276 Size: 1727 662 1572 2961 Fragments arranged by size: 2961 1727 1572 662 ApaLI G'TGCA_C Cuts at: 4979 6135 4979 Size: 1156 5766 ApoI r'AATT_y Cuts at: 1493 2516 3021 3396 3513 3527 3675 3748 3911 Size: 1023 505 375 117 14 148 73 163 Cuts at: 3911 3937 4231 4457 4609 6718 1493 Size: 26 294 226 152 2109 1697 Fragments arranged by size: 2109 1697 1023 505 375 294 226 163 152 148 117 73 26 14 AseI AT'TA_AT Cuts at: 160 1032 5476 160 Size: 872 4444 1606 Fragments arranged by size: 4444 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 AvaI C'yCGr_G Cuts at: 1870 1943 4247 1870 Size: 73 2304 4545 Fragments arranged by size: 4545 2304 73 BamHI G'GATC_C Cuts at: 4252 4619 4252 Size: 367 6555 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4103 4243 5698 618 Size: 325 217 627 2316 140 1455 1842 Fragments arranged by size: 2316 1842 1455 627 325 217 140 BanII G_rGCy'C Cuts at: 1391 2057 4241 1391 Size: 666 2184 4072 Fragments arranged by size: 4072 2184 666 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3713 928 Size: 971 1814 4137 Fragments arranged by size: 4137 1814 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5113 5147 1903 Size: 34 50 34 3092 34 3678 Fragments arranged by size: 3678 3092 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4814 6024 4814 Size: 1210 5712 BclI T'GATC_A Cuts at: 1548 1548 Size: 6922 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5533 136 Size: 107 122 71 631 605 3861 1525 Fragments arranged by size: 3861 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6917 6917 Size: 6922 BlpI GC'TnA_GC Cuts at: 4182 4339 4182 Size: 157 6765 Bme1580I G_kGCm'C Cuts at: 1790 4983 6139 1790 Size: 3193 1156 2573 Fragments arranged by size: 3193 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6922 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2197 4054 4653 Size: 416 53 83 399 24 780 1857 599 Cuts at: 4653 5611 442 Size: 958 1753 Fragments arranged by size: 1857 1753 958 780 599 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6922 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2697 5582 1239 Size: 1458 2885 2579 Fragments arranged by size: 2885 2579 1458 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5047 5912 6210 5047 Size: 865 298 5759 Fragments arranged by size: 5759 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2328 3369 5585 882 Size: 617 829 1041 2216 2219 Fragments arranged by size: 2219 2216 1041 829 617 BsaAI yAC'GTr Cuts at: 493 1204 4713 493 Size: 711 3509 2702 Fragments arranged by size: 3509 2702 711 BsaBI GATnn'nnATC Cuts at: 6916 6916 Size: 6922 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4729 5111 5746 275 Size: 53 83 186 936 3196 382 635 1451 Fragments arranged by size: 3196 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3661 3839 5355 5753 6027 6174 6585 3661 Size: 178 1516 398 274 147 411 3998 Fragments arranged by size: 3998 1516 411 398 274 178 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2042 2072 3799 3829 1178 Size: 30 165 30 639 30 1727 30 4271 Fragments arranged by size: 4271 1727 639 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2126 2475 1186 Size: 937 3 349 5633 Fragments arranged by size: 5633 937 349 3 BseYI C'CCAG_C Cuts at: 6125 6621 6125 Size: 496 6426 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2961 1163 Size: 573 1225 5124 Fragments arranged by size: 5124 1225 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4372 5133 6161 1540 Size: 275 186 2371 761 1028 2301 Fragments arranged by size: 2371 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4023 4983 5068 6139 4023 Size: 960 85 1071 4806 Fragments arranged by size: 4806 1071 960 85 BsmI GAATG_Cn' Cuts at: 2868 2910 3117 4433 4526 2868 Size: 42 207 1316 93 5264 Fragments arranged by size: 5264 1316 207 93 42 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 4023 4241 4983 5068 6139 1391 Size: 399 267 1966 218 742 85 1071 2174 Fragments arranged by size: 2174 1966 1071 742 399 267 218 85 BspEI T'CCGG_A Cuts at: 3661 6585 3661 Size: 2924 3998 BspHI T'CATG_A Cuts at: 3726 4811 6541 3726 Size: 1085 1730 4107 Fragments arranged by size: 4107 1730 1085 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3049 4277 844 Size: 2205 1228 3489 Fragments arranged by size: 3489 2205 1228 BsrBI CCG'CTC Cuts at: 1394 1823 3710 4170 4809 1394 Size: 429 1887 460 639 3507 Fragments arranged by size: 3507 1887 639 460 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5417 5591 65 Size: 1183 4169 174 1396 Fragments arranged by size: 4169 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3839 5566 5753 1700 Size: 110 2029 1727 187 2869 Fragments arranged by size: 2869 2029 1727 187 110 BsrGI T'GTAC_A Cuts at: 96 3214 96 Size: 3118 3804 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6922 BssSI C'ACGA_G Cuts at: 4982 5994 4982 Size: 1012 5910 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6922 BstBI TT'CG_AA Cuts at: 3673 5726 3673 Size: 2053 4869 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6922 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6922 BstYI r'GATC_y Cuts at: 1972 2194 4252 4619 5004 5021 6473 6559 6571 Size: 222 2058 367 385 17 1452 86 12 Cuts at: 6571 6917 1972 Size: 346 1977 Fragments arranged by size: 2058 1977 1452 385 367 346 222 86 17 12 BstZ17I GTA'TAC Cuts at: 3320 3320 Size: 6922 BtgI C'CryG_G Cuts at: 513 1065 2030 4107 513 Size: 552 965 2077 3328 Fragments arranged by size: 3328 2077 965 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6183 Fragments arranged by size: 6183 683 56 BtsI GCAGTG_nn' Cuts at: 3354 3741 4438 5232 5252 3354 Size: 387 697 794 20 5024 Fragments arranged by size: 5024 794 697 387 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6922 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2856 2891 529 Size: 35 2292 35 4560 Fragments arranged by size: 4560 2292 35 35 DraI TTT'AAA Cuts at: 2217 3037 3771 4226 2217 Size: 820 734 455 4913 Fragments arranged by size: 4913 820 734 455 DraIII CAC_nnn'GTG Cuts at: 3128 3923 3128 Size: 795 6127 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5929 809 Size: 507 4613 1802 Fragments arranged by size: 4613 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4369 5258 6887 Size: 54 1425 50 275 18 2539 889 1629 Cuts at: 6887 8 Size: 43 Fragments arranged by size: 2539 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4369 1537 Size: 275 2557 4090 Fragments arranged by size: 4090 2557 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3010 4852 1353 Size: 1657 1842 3423 Fragments arranged by size: 3423 1842 1657 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2039 3018 5509 5883 6029 387 Size: 1064 138 450 979 2491 374 146 1280 Fragments arranged by size: 2491 1280 1064 979 450 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2151 2442 2697 2904 3078 4985 5582 Size: 393 519 291 255 207 174 1907 597 Cuts at: 5582 6369 6637 1239 Size: 787 268 1524 Fragments arranged by size: 1907 1524 787 597 519 393 291 268 255 207 174 EcoNI CCTnn'n_nnAGG Cuts at: 2808 2808 Size: 6922 EcoO109I rG'GnC_Cy Cuts at: 3961 3961 Size: 6922 EcoRI G'AATT_C Cuts at: 3937 4231 4609 3937 Size: 294 378 6250 Fragments arranged by size: 6250 378 294 EcoRV GAT'ATC Cuts at: 2365 2365 Size: 6922 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2269 2873 2269 Size: 604 6318 FspI TGC'GCA Cuts at: 5428 5428 Size: 6922 HaeII r_GCGC'y Cuts at: 1678 2070 6069 1678 Size: 392 3999 2531 Fragments arranged by size: 3999 2531 392 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2042 2074 2744 2776 3953 Size: 32 135 32 470 32 670 32 1177 Cuts at: 3953 3985 3997 4029 5563 5595 5637 5669 1373 Size: 32 12 32 1534 32 42 32 2626 Fragments arranged by size: 2626 1534 1177 670 470 135 42 32 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 2631 4266 4512 669 Size: 988 281 693 1635 246 3079 Fragments arranged by size: 3079 1635 988 693 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6922 HpaI GTT'AAC Cuts at: 2631 4512 2631 Size: 1881 5041 KpnI G_GTAC'C Cuts at: 4247 4247 Size: 6922 MfeI C'AATT_G Cuts at: 3222 4521 3222 Size: 1299 5623 MluI A'CGCG_T Cuts at: 6798 6798 Size: 6922 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2085 6036 6220 1305 Size: 780 3951 184 2007 Fragments arranged by size: 3951 2007 780 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5100 Fragments arranged by size: 5100 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4880 5239 5398 518 Size: 648 3714 359 159 2042 Fragments arranged by size: 3714 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2020 3703 4186 4338 5015 6163 6408 Size: 405 9 1683 483 152 677 1148 245 Cuts at: 6408 1606 Size: 2120 Fragments arranged by size: 2120 1683 1148 677 483 405 245 152 9 NcoI C'CATG_G Cuts at: 513 1065 4107 513 Size: 552 3042 3328 Fragments arranged by size: 3328 3042 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6922 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6922 NotI GC'GGCC_GC Cuts at: 4369 4369 Size: 6922 NspI r_CATG'y Cuts at: 1132 1229 4280 5825 1132 Size: 97 3051 1545 2229 Fragments arranged by size: 3051 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6922 PciI A'CATG_T Cuts at: 5821 5821 Size: 6922 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6922 PmeI GTTT'AAAC Cuts at: 4226 4226 Size: 6922 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2325 2357 2480 2512 4991 5023 6504 6536 2325 Size: 32 123 32 2479 32 1481 32 2711 Fragments arranged by size: 2711 2479 1481 123 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3961 3961 Size: 6922 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6922 PsiI TTA'TAA Cuts at: 4492 4492 Size: 6922 PstI C_TGCA'G Cuts at: 830 4274 830 Size: 3444 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6922 PvuII CAG'CTG Cuts at: 2020 3703 2020 Size: 1683 5239 SalI G'TCGA_C Cuts at: 1936 4264 1936 Size: 2328 4594 SbfI CC_TGCA'GG Cuts at: 4274 4274 Size: 6922 ScaI AGT'ACT Cuts at: 5170 6882 5170 Size: 1712 5210 SexAI A'CCwGG_T Cuts at: 3963 3963 Size: 6922 SfcI C'TryA_G Cuts at: 826 1045 4270 5405 6086 6277 826 Size: 219 3225 1135 681 191 1471 Fragments arranged by size: 3225 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6922 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6922 SmaI CCC'GGG Cuts at: 1872 4249 1872 Size: 2377 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5026 5927 6189 820 Size: 197 926 3083 901 262 1553 Fragments arranged by size: 3083 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4713 493 Size: 4220 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6922 SphI G_CATG'C Cuts at: 1132 4280 1132 Size: 3148 3774 SspI AAT'ATT Cuts at: 5 52 3888 4846 5 Size: 47 3836 958 2081 Fragments arranged by size: 3836 2081 958 47 StuI AGG'CCT Cuts at: 1517 3055 1517 Size: 1538 5384 StyI C'CwwG_G Cuts at: 513 1065 2809 4031 4107 4361 513 Size: 552 1744 1222 76 254 3074 Fragments arranged by size: 3074 1744 1222 552 254 76 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2352 4949 4966 5119 5304 5749 325 Size: 1255 772 2597 17 153 185 445 1498 Fragments arranged by size: 2597 1498 1255 772 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3214 5168 6880 Size: 274 80 33 51 1443 1237 1954 1712 Cuts at: 6880 96 Size: 138 Fragments arranged by size: 1954 1712 1443 1237 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2790 2822 3450 3482 3799 3831 6882 6914 2790 Size: 32 628 32 317 32 3051 32 2798 Fragments arranged by size: 3051 2798 628 317 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6922 XbaI T'CTAG_A Cuts at: 4258 4258 Size: 6922 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6922 XmaI C'CCGG_G Cuts at: 1870 4247 1870 Size: 2377 4545 XmnI GAAnn'nnTTC Cuts at: 3399 3588 3669 5051 3399 Size: 189 81 1382 5270 Fragments arranged by size: 5270 1382 189 81 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4730 276 Size: 53 83 186 936 3196 2468 Fragments arranged by size: 3196 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI