(Circular) (Six-base) MAPSORT of: pFN21AB3305.seq Check: 6697 from: 1 to: 6436 pFN21AB3305 6436 bp With 182 enzymes: SgfI * October 20, 2009 10:44 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2705 3220 2705 Size: 515 5921 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3779 1937 Size: 1842 4594 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 4563 4936 1563 Size: 3000 373 3063 Fragments arranged by size: 3063 3000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2471 2861 2921 4499 5883 6151 1632 Size: 839 390 60 1578 1384 268 1917 Fragments arranged by size: 1917 1578 1384 839 390 268 60 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6239 AflIII A'CryG_T Cuts at: 1203 3481 5335 6312 1203 Size: 2278 1854 977 1327 Fragments arranged by size: 2278 1854 1327 977 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 5165 5165 Size: 6436 AleI CACnn'nnGTG Cuts at: 1166 3480 1166 Size: 2314 4122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6436 AlwNI CAG_nnn'CTG Cuts at: 3500 4179 5751 3500 Size: 679 1572 4185 Fragments arranged by size: 4185 1572 679 ApaI G_GGCC'C Cuts at: 2566 2566 Size: 6436 ApaLI G'TGCA_C Cuts at: 2741 4493 5649 2741 Size: 1752 1156 3528 Fragments arranged by size: 3528 1752 1156 ApoI r'AATT_y Cuts at: 1493 2250 3547 3745 3971 4123 6232 1493 Size: 757 1297 198 226 152 2109 1697 Fragments arranged by size: 2109 1697 1297 757 226 198 152 AseI AT'TA_AT Cuts at: 160 1032 4990 160 Size: 872 3958 1606 Fragments arranged by size: 3958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 2523 3212 3761 1870 Size: 73 580 689 549 4545 Fragments arranged by size: 4545 689 580 549 73 BamHI G'GATC_C Cuts at: 3766 4133 3766 Size: 367 6069 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2224 2502 2608 2627 3257 Size: 325 217 627 437 278 106 19 630 Cuts at: 3257 3757 5212 618 Size: 500 1455 1842 Fragments arranged by size: 1842 1455 630 627 500 437 325 278 217 106 19 BanII G_rGCy'C Cuts at: 1391 2546 2566 3755 1391 Size: 1155 20 1189 4072 Fragments arranged by size: 4072 1189 1155 20 BbeI G_GCGC'C Cuts at: 2506 3261 2506 Size: 755 5681 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5465 BbvCI CC'TCA_GC Cuts at: 3561 3561 Size: 6436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4627 4661 1903 Size: 34 2690 34 3678 Fragments arranged by size: 3678 2690 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4328 5538 4328 Size: 1210 5226 BclI T'GATC_A Cuts at: 1548 1548 Size: 6436 BfrBI ATG'CAT Cuts at: 2797 2797 Size: 6436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2511 5047 136 Size: 107 122 71 631 605 839 2536 1525 Fragments arranged by size: 2536 1525 839 631 605 122 107 71 BglII A'GATC_T Cuts at: 2082 6431 2082 Size: 4349 2087 BlpI GC'TnA_GC Cuts at: 3853 3853 Size: 6436 Bme1580I G_kGCm'C Cuts at: 1790 2229 2566 2613 2745 4497 5653 1790 Size: 439 337 47 132 1752 1156 2573 Fragments arranged by size: 2573 1752 1156 439 337 132 47 BmgBI CAC'GTC Cuts at: 1318 2695 1318 Size: 1377 5059 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2158 2611 2674 Size: 416 53 83 399 24 741 453 63 Cuts at: 2674 3186 4167 5125 442 Size: 512 981 958 1753 Fragments arranged by size: 1753 981 958 741 512 453 416 399 83 63 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6436 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2779 2779 Size: 6436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3488 5096 1239 Size: 2249 1608 2579 Fragments arranged by size: 2579 2249 1608 Bpu10I CC'TnA_GC Cuts at: 2808 3402 3561 2808 Size: 594 159 5683 Fragments arranged by size: 5683 594 159 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3410 4561 5426 5724 3410 Size: 1151 865 298 4122 Fragments arranged by size: 4122 1151 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2461 3464 3573 5099 882 Size: 617 962 1003 109 1526 2219 Fragments arranged by size: 2219 1526 1003 962 617 109 BsaAI yAC'GTr Cuts at: 493 1204 3482 4227 493 Size: 711 2278 745 2702 Fragments arranged by size: 2702 2278 745 711 BsaBI GATnn'nnATC Cuts at: 6430 6430 Size: 6436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2503 2872 3258 4243 Size: 53 83 186 936 970 369 386 985 Cuts at: 4243 4625 5260 275 Size: 382 635 1451 Fragments arranged by size: 1451 985 970 936 635 386 382 369 186 83 53 BsaWI w'CCGG_w Cuts at: 4869 5267 5541 5688 6099 4869 Size: 398 274 147 411 5206 Fragments arranged by size: 5206 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2051 2069 2081 2099 2262 Size: 30 165 30 648 18 12 18 163 Cuts at: 2262 2292 1178 Size: 30 5322 Fragments arranged by size: 5322 648 165 163 30 30 30 18 18 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2755 2932 3446 1186 Size: 1569 177 514 4176 Fragments arranged by size: 4176 1569 514 177 BseYI C'CCAG_C Cuts at: 2274 2566 3133 3164 5639 6135 2274 Size: 292 567 31 2475 496 2575 Fragments arranged by size: 2575 2475 567 496 292 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2739 3248 1163 Size: 573 1003 509 4351 Fragments arranged by size: 4351 1003 573 509 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3886 4647 5675 1540 Size: 275 186 1885 761 1028 2301 Fragments arranged by size: 2301 1885 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2079 2148 2745 4497 4582 5653 2079 Size: 69 597 1752 85 1071 2862 Fragments arranged by size: 2862 1752 1071 597 85 69 BsmI GAATG_Cn' Cuts at: 3947 4040 3947 Size: 93 6343 BsmBI CGTCTCn'nnnn_ Cuts at: 2351 2879 2351 Size: 528 5908 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2148 2229 2546 2566 2613 2745 Size: 399 289 69 81 317 20 47 132 Cuts at: 2745 3755 4497 4582 5653 1391 Size: 1010 742 85 1071 2174 Fragments arranged by size: 2174 1071 1010 742 399 317 289 132 85 81 69 47 20 BspEI T'CCGG_A Cuts at: 6099 6099 Size: 6436 BspHI T'CATG_A Cuts at: 2412 4325 6055 2412 Size: 1913 1730 2793 Fragments arranged by size: 2793 1913 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2705 3220 3791 844 Size: 1861 515 571 3489 Fragments arranged by size: 3489 1861 571 515 BsrBI CCG'CTC Cuts at: 1394 1823 4323 1394 Size: 429 2500 3507 Fragments arranged by size: 3507 2500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4931 5105 65 Size: 1183 3683 174 1396 Fragments arranged by size: 3683 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5080 5267 1700 Size: 110 3270 187 2869 Fragments arranged by size: 3270 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6436 BssSI C'ACGA_G Cuts at: 4496 5508 4496 Size: 1012 5424 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6436 BstBI TT'CG_AA Cuts at: 5240 5240 Size: 6436 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstXI CCAn_nnnn'nTGG Cuts at: 3646 3646 Size: 6436 BstYI r'GATC_y Cuts at: 1972 2082 2844 3660 3766 4133 4518 4535 5987 Size: 110 762 816 106 367 385 17 1452 Cuts at: 5987 6073 6085 6431 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 816 762 385 367 346 110 106 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2754 2754 Size: 6436 BtgI C'CryG_G Cuts at: 513 1065 2049 513 Size: 552 984 4900 Fragments arranged by size: 4900 984 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2168 487 Size: 683 56 942 4755 Fragments arranged by size: 4755 942 683 56 BtsI GCAGTG_nn' Cuts at: 2370 3258 3952 4746 4766 2370 Size: 888 694 794 20 4040 Fragments arranged by size: 4040 888 794 694 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3740 3740 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5443 809 Size: 507 4127 1802 Fragments arranged by size: 4127 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2425 3502 Size: 54 1425 50 275 18 265 330 1077 Cuts at: 3502 3883 4772 6401 8 Size: 381 889 1629 43 Fragments arranged by size: 1629 1425 1077 889 381 330 275 265 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3883 1537 Size: 275 2071 4090 Fragments arranged by size: 4090 2071 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4366 1353 Size: 3013 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5023 5397 5543 387 Size: 1064 138 3434 374 146 1280 Fragments arranged by size: 3434 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2471 2861 2921 3488 4499 5096 5883 Size: 393 839 390 60 567 1011 597 787 Cuts at: 5883 6151 1239 Size: 268 1524 Fragments arranged by size: 1524 1011 839 787 597 567 393 390 268 60 EcoNI CCTnn'n_nnAGG Cuts at: 2903 3677 2903 Size: 774 5662 EcoO109I rG'GnC_Cy Cuts at: 2489 2562 2563 2489 Size: 73 1 6362 Fragments arranged by size: 6362 73 1 EcoRI G'AATT_C Cuts at: 3745 4123 3745 Size: 378 6058 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2800 2800 Size: 6436 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 2506 3261 3315 5583 1678 Size: 828 755 54 2268 2531 Fragments arranged by size: 2531 2268 828 755 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5077 5109 5151 5183 1373 Size: 32 135 32 3505 32 42 32 2626 Fragments arranged by size: 3505 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3780 4026 669 Size: 988 281 1842 246 3079 Fragments arranged by size: 3079 1842 988 281 246 HindIII A'AGCT_T Cuts at: 748 2012 748 Size: 1264 5172 HpaI GTT'AAC Cuts at: 4026 4026 Size: 6436 KasI G'GCGC_C Cuts at: 2502 3257 2502 Size: 755 5681 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 4035 4035 Size: 6436 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2409 2415 5550 5734 1305 Size: 1104 6 3135 184 2007 Fragments arranged by size: 3135 2007 1104 184 6 MscI TGG'CCA Cuts at: 10 64 1489 1832 2427 10 Size: 54 1425 343 595 4019 Fragments arranged by size: 4019 1425 595 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2738 3480 3482 3633 4394 4753 4912 Size: 648 1572 742 2 151 761 359 159 Cuts at: 4912 518 Size: 2042 Fragments arranged by size: 2042 1572 761 742 648 359 159 151 2 MspA1I CmG'CkG Cuts at: 1606 2051 2094 2558 2723 3500 3560 3852 4529 Size: 445 43 464 165 777 60 292 677 Cuts at: 4529 5677 5922 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 777 677 464 445 292 245 165 60 43 NarI GG'CG_CC Cuts at: 2503 3258 2503 Size: 755 5681 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5884 NdeI CA'TA_TG Cuts at: 387 387 Size: 6436 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6436 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NsiI A_TGCA'T Cuts at: 2799 2799 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 3794 5339 1132 Size: 97 2565 1545 2229 Fragments arranged by size: 2565 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6436 PciI A'CATG_T Cuts at: 5335 5335 Size: 6436 PflMI CCAn_nnn'nTGG Cuts at: 1108 2104 2434 2623 3253 1108 Size: 996 330 189 630 4291 Fragments arranged by size: 4291 996 630 330 189 PfoI T'CCnGG_A Cuts at: 2177 2177 Size: 6436 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2489 2489 Size: 6436 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6436 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PspOMI G'GGCC_C Cuts at: 2562 2562 Size: 6436 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 6404 PstI C_TGCA'G Cuts at: 830 3087 3788 830 Size: 2257 701 3478 Fragments arranged by size: 3478 2257 701 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 PvuII CAG'CTG Cuts at: 2723 3500 3560 2723 Size: 777 60 5599 Fragments arranged by size: 5599 777 60 SacII CC_GC'GG Cuts at: 2052 2052 Size: 6436 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SbfI CC_TGCA'GG Cuts at: 3788 3788 Size: 6436 ScaI AGT'ACT Cuts at: 2292 4684 6396 2292 Size: 2392 1712 2332 Fragments arranged by size: 2392 2332 1712 SexAI A'CCwGG_T Cuts at: 3603 3603 Size: 6436 SfcI C'TryA_G Cuts at: 826 1045 2089 3083 3120 3190 3784 4919 5600 Size: 219 1044 994 37 70 594 1135 681 Cuts at: 5600 5791 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1044 994 681 594 219 191 70 37 SfoI GGC'GCC Cuts at: 2504 3259 2504 Size: 755 5681 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 3763 1872 Size: 1891 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3425 4540 5441 5703 820 Size: 197 926 1482 1115 901 262 1553 Fragments arranged by size: 1553 1482 1115 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 3794 1132 Size: 2662 3774 SspI AAT'ATT Cuts at: 5 52 2266 4360 5 Size: 47 2214 2094 2081 Fragments arranged by size: 2214 2094 2081 47 StuI AGG'CCT Cuts at: 1517 3053 1517 Size: 1536 4900 StyI C'CwwG_G Cuts at: 513 1065 3450 3875 513 Size: 552 2385 425 3074 Fragments arranged by size: 3074 2385 552 425 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2615 2630 4463 4480 4633 4818 5263 Size: 1255 1035 15 1833 17 153 185 445 Cuts at: 5263 325 Size: 1498 Fragments arranged by size: 1833 1498 1255 1035 445 185 153 17 15 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2290 4682 6394 Size: 274 80 33 51 1443 313 2392 1712 Cuts at: 6394 96 Size: 138 Fragments arranged by size: 2392 1712 1443 313 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2069 2101 2262 2294 3516 3548 6396 6428 2069 Size: 32 161 32 1222 32 2848 32 2077 Fragments arranged by size: 2848 2077 1222 161 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2680 3605 1316 Size: 1364 925 4147 Fragments arranged by size: 4147 1364 925 XbaI T'CTAG_A Cuts at: 3772 3772 Size: 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2620 3358 2620 Size: 738 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6436 XmaI C'CCGG_G Cuts at: 1870 3761 1870 Size: 1891 4545 XmnI GAAnn'nnTTC Cuts at: 4565 4565 Size: 6436 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BsiWI BstZ17I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspXI RsrII SacI SanDI SapI SfiI SrfI SwaI