Restriction map for
pFN21AB3305
(Linear) (Six-base) MAPSORT of: KIBB3305.seq Check: 4746 from: 1 to: 1731
KIBB3305.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 700 1215 1731
Size: 700 515 516
Fragments arranged by size:
700 516 515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 856 916 1731
Size: 466 390 60 815
Fragments arranged by size:
815 466 390 60
AflIII A'CryG_T
Cuts at: 0 1476 1731
Size: 1476 255
AleI CACnn'nnGTG
Cuts at: 0 1475 1731
Size: 1475 256
AlwNI CAG_nnn'CTG
Cuts at: 0 1495 1731
Size: 1495 236
ApaI G_GGCC'C
Cuts at: 0 561 1731
Size: 561 1170
ApaLI G'TGCA_C
Cuts at: 0 736 1731
Size: 736 995
ApoI r'AATT_y
Cuts at: 0 245 1542 1731
Size: 245 1297 189
Fragments arranged by size:
1297 245 189
AvaI C'yCGr_G
Cuts at: 0 518 1207 1731
Size: 518 689 524
Fragments arranged by size:
689 524 518
BanI G'GyrC_C
Cuts at: 0 219 497 603 622 1252 1731
Size: 219 278 106 19 630 479
Fragments arranged by size:
630 479 278 219 106 19
BanII G_rGCy'C
Cuts at: 0 541 561 1731
Size: 541 20 1170
Fragments arranged by size:
1170 541 20
BbeI G_GCGC'C
Cuts at: 0 501 1256 1731
Size: 501 755 475
Fragments arranged by size:
755 501 475
BbvCI CC'TCA_GC
Cuts at: 0 1556 1731
Size: 1556 175
BfrBI ATG'CAT
Cuts at: 0 792 1731
Size: 792 939
BglI GCCn_nnn'nGGC
Cuts at: 0 506 1731
Size: 506 1225
BglII A'GATC_T
Cuts at: 0 77 1731
Size: 77 1654
Bme1580I G_kGCm'C
Cuts at: 0 224 561 608 740 1731
Size: 224 337 47 132 991
Fragments arranged by size:
991 337 224 132 47
BmgBI CAC'GTC
Cuts at: 0 690 1731
Size: 690 1041
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 606 669 1181 1731
Size: 153 453 63 512 550
Fragments arranged by size:
550 512 453 153 63
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 774 1731
Size: 774 957
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1483 1731
Size: 1483 248
Bpu10I CC'TnA_GC
Cuts at: 0 803 1397 1556 1731
Size: 803 594 159 175
Fragments arranged by size:
803 594 175 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1405 1731
Size: 1405 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 456 1459 1568 1731
Size: 456 1003 109 163
Fragments arranged by size:
1003 456 163 109
BsaAI yAC'GTr
Cuts at: 0 1477 1731
Size: 1477 254
BsaHI Gr'CG_yC
Cuts at: 0 498 867 1253 1731
Size: 498 369 386 478
Fragments arranged by size:
498 478 386 369
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 64 76 94 257 287 1731
Size: 46 18 12 18 163 30 1444
Fragments arranged by size:
1444 163 46 30 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 750 927 1441 1731
Size: 750 177 514 290
Fragments arranged by size:
750 514 290 177
BseYI C'CCAG_C
Cuts at: 0 269 561 1128 1159 1731
Size: 269 292 567 31 572
Fragments arranged by size:
572 567 292 269 31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 734 1243 1731
Size: 734 509 488
Fragments arranged by size:
734 509 488
BsiHKAI G_wGCw'C
Cuts at: 0 74 143 740 1731
Size: 74 69 597 991
Fragments arranged by size:
991 597 74 69
BsmBI CGTCTCn'nnnn_
Cuts at: 0 346 874 1731
Size: 346 528 857
Fragments arranged by size:
857 528 346
Bsp1286I G_dGCh'C
Cuts at: 0 74 143 224 541 561 608 740 1731
Size: 74 69 81 317 20 47 132 991
Fragments arranged by size:
991 317 132 81 74 69 47 20
BspHI T'CATG_A
Cuts at: 0 407 1731
Size: 407 1324
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 700 1215 1731
Size: 700 515 516
Fragments arranged by size:
700 516 515
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1641 1731
Size: 1641 90
BstYI r'GATC_y
Cuts at: 0 77 839 1655 1731
Size: 77 762 816 76
Fragments arranged by size:
816 762 77 76
Bsu36I CC'TnA_GG
Cuts at: 0 749 1731
Size: 749 982
BtgI C'CryG_G
Cuts at: 0 44 1731
Size: 44 1687
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 163 1731
Size: 163 1568
BtsI GCAGTG_nn'
Cuts at: 0 365 1253 1731
Size: 365 888 478
Fragments arranged by size:
888 478 365
EaeI y'GGCC_r
Cuts at: 0 90 420 1497 1731
Size: 90 330 1077 234
Fragments arranged by size:
1077 330 234 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 466 856 916 1483 1731
Size: 466 390 60 567 248
Fragments arranged by size:
567 466 390 248 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 898 1672 1731
Size: 898 774 59
Fragments arranged by size:
898 774 59
EcoO109I rG'GnC_Cy
Cuts at: 0 484 557 558 1731
Size: 484 73 1 1173
Fragments arranged by size:
1173 484 73 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 795 1731
Size: 795 936
HaeII r_GCGC'y
Cuts at: 0 501 1256 1310 1731
Size: 501 755 54 421
Fragments arranged by size:
755 501 421 54
HindIII A'AGCT_T
Cuts at: 0 7 1731
Size: 7 1724
KasI G'GCGC_C
Cuts at: 0 497 1252 1731
Size: 497 755 479
Fragments arranged by size:
755 497 479
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 404 410 1731
Size: 404 6 1321
Fragments arranged by size:
1321 404 6
MscI TGG'CCA
Cuts at: 0 422 1731
Size: 422 1309
MslI CAynn'nnrTG
Cuts at: 0 733 1475 1477 1628 1731
Size: 733 742 2 151 103
Fragments arranged by size:
742 733 151 103 2
MspA1I CmG'CkG
Cuts at: 0 46 89 553 718 1495 1555 1731
Size: 46 43 464 165 777 60 176
Fragments arranged by size:
777 464 176 165 60 46 43
NarI GG'CG_CC
Cuts at: 0 498 1253 1731
Size: 498 755 478
Fragments arranged by size:
755 498 478
NsiI A_TGCA'T
Cuts at: 0 794 1731
Size: 794 937
PflMI CCAn_nnn'nTGG
Cuts at: 0 99 429 618 1248 1731
Size: 99 330 189 630 483
Fragments arranged by size:
630 483 330 189 99
PfoI T'CCnGG_A
Cuts at: 0 172 1731
Size: 172 1559
PpuMI rG'GwC_Cy
Cuts at: 0 484 1731
Size: 484 1247
PspOMI G'GGCC_C
Cuts at: 0 557 1731
Size: 557 1174
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 27 1731
Size: 27 1704
PstI C_TGCA'G
Cuts at: 0 1082 1731
Size: 1082 649
PvuII CAG'CTG
Cuts at: 0 718 1495 1555 1731
Size: 718 777 60 176
Fragments arranged by size:
777 718 176 60
SacII CC_GC'GG
Cuts at: 0 47 1731
Size: 47 1684
ScaI AGT'ACT
Cuts at: 0 287 1731
Size: 287 1444
SexAI A'CCwGG_T
Cuts at: 0 1598 1731
Size: 1598 133
SfcI C'TryA_G
Cuts at: 0 84 1078 1115 1185 1731
Size: 84 994 37 70 546
Fragments arranged by size:
994 546 84 70 37
SfoI GGC'GCC
Cuts at: 0 499 1254 1731
Size: 499 755 477
Fragments arranged by size:
755 499 477
SmlI C'TyrA_G
Cuts at: 0 1420 1731
Size: 1420 311
SspI AAT'ATT
Cuts at: 0 261 1731
Size: 261 1470
StuI AGG'CCT
Cuts at: 0 1048 1731
Size: 1048 683
StyI C'CwwG_G
Cuts at: 0 1445 1731
Size: 1445 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 610 625 1731
Size: 610 15 1106
Fragments arranged by size:
1106 610 15
TatI w'GTAC_w
Cuts at: 0 285 1731
Size: 285 1446
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 64 96 257 289 1511 1543 1731
Size: 64 32 161 32 1222 32 188
Fragments arranged by size:
1222 188 161 64 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 675 1600 1731
Size: 675 925 131
Fragments arranged by size:
925 675 131
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 615 1353 1731
Size: 615 738 378
Fragments arranged by size:
738 615 378
Enzymes that do cut:
AarI AcuI AflIII AleI AlwNI ApaI ApaLI ApoI
AvaI BanI BanII BbeI BbvCI BfrBI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BstXI BstYI Bsu36I BtgI BtgZI
BtsI EaeI Eco57MI EcoNI EcoO109I FalI HaeII HindIII
KasI MmeI MscI MslI MspA1I NarI NsiI PflMI
PfoI PpuMI PspOMI PsrI PstI PvuII SacII ScaI
SexAI SfcI SfoI SmlI SspI StuI StyI TaqII
TatI TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BcgI BciVI BclI BlpI BmtI BsaBI BsaWI
BsiEI BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HpaI KpnI
MfeI MluI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PvuI RsrII SacI SalI SanDI
SapI SbfI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI XbaI XhoI XmaI XmnI ZraI