(Circular) (Six-base) MAPSORT of: pFN21AB3281.seq Check: 6716 from: 1 to: 7066 pFN21AB3281 7066 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2617 2617 Size: 7066 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4876 278 Size: 53 83 186 936 3340 2468 Fragments arranged by size: 3340 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4038 4409 1937 Size: 2101 371 4594 Fragments arranged by size: 4594 2101 371 Acc65I G'GTAC_C Cuts at: 4387 4387 Size: 7066 AclI AA'CG_TT Cuts at: 1563 5193 5566 1563 Size: 3630 373 3063 Fragments arranged by size: 3630 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 3050 3235 5129 6513 6781 1632 Size: 641 777 185 1894 1384 268 1917 Fragments arranged by size: 1917 1894 1384 777 641 268 185 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6869 AflIII A'CryG_T Cuts at: 1203 2292 4008 4227 5965 6942 1203 Size: 1089 1716 219 1738 977 1327 Fragments arranged by size: 1738 1716 1327 1089 977 219 AgeI A'CCGG_T Cuts at: 5897 5897 Size: 7066 AhdI GACnn_n'nnGTC Cuts at: 5795 5795 Size: 7066 AleI CACnn'nnGTG Cuts at: 1166 3523 1166 Size: 2357 4709 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7066 AlwNI CAG_nnn'CTG Cuts at: 2185 2206 2668 2722 2785 2911 3028 3056 3476 Size: 21 462 54 63 126 117 28 420 Cuts at: 3476 3613 4809 6381 2185 Size: 137 1196 1572 2870 Fragments arranged by size: 2870 1572 1196 462 420 137 126 117 63 54 28 21 ApaI G_GGCC'C Cuts at: 2805 2805 Size: 7066 ApaLI G'TGCA_C Cuts at: 3089 5123 6279 3089 Size: 2034 1156 3876 Fragments arranged by size: 3876 2034 1156 ApoI r'AATT_y Cuts at: 1493 3320 3545 3688 4375 4601 4753 6862 1493 Size: 1827 225 143 687 226 152 2109 1697 Fragments arranged by size: 2109 1827 1697 687 226 225 152 143 AseI AT'TA_AT Cuts at: 160 1032 5620 160 Size: 872 4588 1606 Fragments arranged by size: 4588 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7066 AvaI C'yCGr_G Cuts at: 1870 1943 2807 4391 1870 Size: 73 864 1584 4545 Fragments arranged by size: 4545 1584 864 73 BamHI G'GATC_C Cuts at: 2547 4347 4396 4763 2547 Size: 1800 49 367 4850 Fragments arranged by size: 4850 1800 367 49 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3233 3484 4387 5842 618 Size: 325 217 627 1446 251 903 1455 1842 Fragments arranged by size: 1842 1455 1446 903 627 325 251 217 BanII G_rGCy'C Cuts at: 1391 2585 2805 4320 4385 1391 Size: 1194 220 1515 65 4072 Fragments arranged by size: 4072 1515 1194 220 65 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2714 3038 928 Size: 971 815 324 4956 Fragments arranged by size: 4956 971 815 324 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5257 5291 1903 Size: 34 3320 34 3678 Fragments arranged by size: 3678 3320 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2010 4091 4958 6168 2010 Size: 2081 867 1210 2908 Fragments arranged by size: 2908 2081 1210 867 BclI T'GATC_A Cuts at: 1548 1548 Size: 7066 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5677 136 Size: 107 122 71 631 605 4005 1525 Fragments arranged by size: 4005 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2143 3189 7061 2143 Size: 1046 3872 2148 Fragments arranged by size: 3872 2148 1046 BlpI GC'TnA_GC Cuts at: 2025 2579 2892 4483 2025 Size: 554 313 1591 4608 Fragments arranged by size: 4608 1591 554 313 Bme1580I G_kGCm'C Cuts at: 1790 2271 2805 3093 3247 5127 6283 1790 Size: 481 534 288 154 1880 1156 2573 Fragments arranged by size: 2573 1880 1156 534 481 288 154 BmgBI CAC'GTC Cuts at: 1318 2824 1318 Size: 1506 5560 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2104 2921 3337 Size: 416 53 83 399 24 687 817 416 Cuts at: 3337 3667 4797 5755 442 Size: 330 1130 958 1753 Fragments arranged by size: 1753 1130 958 817 687 416 416 399 330 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7066 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2207 2270 2373 2414 2600 2657 3659 4301 Size: 968 63 103 41 186 57 1002 642 Cuts at: 4301 5726 1239 Size: 1425 2579 Fragments arranged by size: 2579 1425 1002 968 642 186 103 63 57 41 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3137 3743 5191 6056 6354 3137 Size: 606 1448 865 298 3849 Fragments arranged by size: 3849 1448 865 606 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5729 882 Size: 617 4230 2219 Fragments arranged by size: 4230 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2295 4009 4102 4176 4857 493 Size: 711 1091 1714 93 74 681 2702 Fragments arranged by size: 2702 1714 1091 711 681 93 74 BsaBI GATnn'nnATC Cuts at: 7060 7060 Size: 7066 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4873 5255 5890 275 Size: 53 83 186 936 3340 382 635 1451 Fragments arranged by size: 3340 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5499 5897 6171 6318 6729 5499 Size: 398 274 147 411 5836 Fragments arranged by size: 5836 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2630 2660 1178 Size: 30 165 30 1227 30 5584 Fragments arranged by size: 5584 1227 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2465 2948 3009 4328 1186 Size: 1279 483 61 1319 3924 Fragments arranged by size: 3924 1319 1279 483 61 BseYI C'CCAG_C Cuts at: 2178 2263 2574 4198 6269 6765 2178 Size: 85 311 1624 2071 496 2479 Fragments arranged by size: 2479 2071 1624 496 311 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2219 3581 1163 Size: 573 483 1362 4648 Fragments arranged by size: 4648 1362 573 483 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3979 4516 5277 6305 1540 Size: 275 186 1978 537 761 1028 2301 Fragments arranged by size: 2301 1978 1028 761 537 275 186 BsiHKAI G_wGCw'C Cuts at: 2226 2898 3093 4320 5127 5212 6283 2226 Size: 672 195 1227 807 85 1071 3009 Fragments arranged by size: 3009 1227 1071 807 672 195 85 BsmI GAATG_Cn' Cuts at: 4577 4670 4577 Size: 93 6973 BsmBI CGTCTCn'nnnn_ Cuts at: 2600 2600 Size: 7066 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2226 2271 2585 2805 2898 3093 3247 Size: 399 436 45 314 220 93 195 154 Cuts at: 3247 4320 4385 5127 5212 6283 1391 Size: 1073 65 742 85 1071 2174 Fragments arranged by size: 2174 1073 1071 742 436 399 314 220 195 154 93 85 65 45 BspEI T'CCGG_A Cuts at: 6729 6729 Size: 7066 BspHI T'CATG_A Cuts at: 4955 6685 4955 Size: 1730 5336 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2617 4421 844 Size: 1773 1804 3489 Fragments arranged by size: 3489 1804 1773 BsrBI CCG'CTC Cuts at: 1394 1823 4953 1394 Size: 429 3130 3507 Fragments arranged by size: 3507 3130 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3178 3725 3888 5561 5735 65 Size: 1183 1930 547 163 1673 174 1396 Fragments arranged by size: 1930 1673 1396 1183 547 174 163 BsrFI r'CCGG_y Cuts at: 1700 1810 2871 4326 5710 5897 1700 Size: 110 1061 1455 1384 187 2869 Fragments arranged by size: 2869 1455 1384 1061 187 110 BsrGI T'GTAC_A Cuts at: 96 2343 96 Size: 2247 4819 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7066 BssSI C'ACGA_G Cuts at: 3593 5126 6138 3593 Size: 1533 1012 4521 Fragments arranged by size: 4521 1533 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2206 2722 794 Size: 1412 516 5138 Fragments arranged by size: 5138 1412 516 BstBI TT'CG_AA Cuts at: 5870 5870 Size: 7066 BstEII G'GTnAC_C Cuts at: 1184 2986 1184 Size: 1802 5264 BstXI CCAn_nnnn'nTGG Cuts at: 2317 2746 4180 2317 Size: 429 1434 5203 Fragments arranged by size: 5203 1434 429 BstYI r'GATC_y Cuts at: 1972 2143 2547 2880 3189 4347 4396 4763 5148 Size: 171 404 333 309 1158 49 367 385 Cuts at: 5148 5165 6617 6703 6715 7061 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1158 404 385 367 346 333 309 171 86 49 17 12 BstZ17I GTA'TAC Cuts at: 4039 4039 Size: 7066 Bsu36I CC'TnA_GG Cuts at: 2543 3021 2543 Size: 478 6588 BtgI C'CryG_G Cuts at: 513 1065 2004 2310 513 Size: 552 939 306 5269 Fragments arranged by size: 5269 939 552 306 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6327 Fragments arranged by size: 6327 683 56 BtsI GCAGTG_nn' Cuts at: 2666 4582 5376 5396 2666 Size: 1916 794 20 4336 Fragments arranged by size: 4336 1916 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7066 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7031 DraI TTT'AAA Cuts at: 4370 4370 Size: 7066 DraIII CAC_nnn'GTG Cuts at: 2230 2230 Size: 7066 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6073 809 Size: 507 4757 1802 Fragments arranged by size: 4757 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2071 2691 2787 Size: 54 1425 50 275 18 241 620 96 Cuts at: 2787 2850 2943 3441 3515 4022 4328 4513 5402 Size: 63 93 498 74 507 306 185 889 Cuts at: 5402 7031 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 620 507 498 306 275 241 185 96 93 74 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4513 1537 Size: 275 2701 4090 Fragments arranged by size: 4090 2701 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4996 1353 Size: 3643 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2321 5653 6027 6173 387 Size: 1064 138 732 3332 374 146 1280 Fragments arranged by size: 3332 1280 1064 732 374 146 138 EcoICRI GAG'CTC Cuts at: 4318 4318 Size: 7066 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2270 2273 2373 2414 2600 2657 Size: 393 575 63 3 100 41 186 57 Cuts at: 2657 3050 3235 3659 4301 5129 5726 6513 6781 Size: 393 185 424 642 828 597 787 268 Cuts at: 6781 1239 Size: 1524 Fragments arranged by size: 1524 828 787 642 597 575 424 393 393 268 186 185 100 63 57 41 3 EcoNI CCTnn'n_nnAGG Cuts at: 2198 3581 2198 Size: 1383 5683 EcoO109I rG'GnC_Cy Cuts at: 2191 2802 3025 3574 3843 4189 2191 Size: 611 223 549 269 346 5068 Fragments arranged by size: 5068 611 549 346 269 223 EcoRI G'AATT_C Cuts at: 4375 4753 4375 Size: 378 6688 FseI GG_CCGG'CC Cuts at: 4330 4330 Size: 7066 FspI TGC'GCA Cuts at: 5572 5572 Size: 7066 HaeII r_GCGC'y Cuts at: 1678 3536 4173 6213 1678 Size: 1858 637 2040 2531 Fragments arranged by size: 2531 2040 1858 637 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2008 2040 2399 2431 2539 Size: 32 135 32 436 32 359 32 108 Cuts at: 2539 2571 2960 2992 5707 5739 5781 5813 1373 Size: 32 389 32 2715 32 42 32 2626 Fragments arranged by size: 2715 2626 436 389 359 135 108 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2953 4410 4656 669 Size: 988 281 1015 1457 246 3079 Fragments arranged by size: 3079 1457 1015 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7066 HpaI GTT'AAC Cuts at: 4656 4656 Size: 7066 KpnI G_GTAC'C Cuts at: 4391 4391 Size: 7066 MfeI C'AATT_G Cuts at: 4665 4665 Size: 7066 MluI A'CGCG_T Cuts at: 6942 6942 Size: 7066 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6180 6364 1305 Size: 4875 184 2007 Fragments arranged by size: 4875 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2073 3517 10 Size: 54 1425 343 241 1444 3559 Fragments arranged by size: 3559 1444 1425 343 241 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2090 3523 3771 4178 5024 5383 Size: 648 843 81 1433 248 407 846 359 Cuts at: 5383 5542 518 Size: 159 2042 Fragments arranged by size: 2042 1433 846 843 648 407 359 248 159 81 MspA1I CmG'CkG Cuts at: 1606 2304 2325 2393 2569 2578 2719 2785 2911 Size: 698 21 68 176 9 141 66 126 Cuts at: 2911 3523 4202 4482 5159 6307 6552 1606 Size: 612 679 280 677 1148 245 2120 Fragments arranged by size: 2120 1148 698 679 677 612 280 245 176 141 126 68 66 21 9 NaeI GCC'GGC Cuts at: 4328 4328 Size: 7066 NcoI C'CATG_G Cuts at: 513 1065 2004 2310 513 Size: 552 939 306 5269 Fragments arranged by size: 5269 939 552 306 NdeI CA'TA_TG Cuts at: 387 387 Size: 7066 NgoMIV G'CCGG_C Cuts at: 4326 4326 Size: 7066 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7066 NotI GC'GGCC_GC Cuts at: 4513 4513 Size: 7066 NspI r_CATG'y Cuts at: 1132 1229 4231 4424 5969 1132 Size: 97 3002 193 1545 2229 Fragments arranged by size: 3002 2229 1545 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7066 PciI A'CATG_T Cuts at: 4227 5965 4227 Size: 1738 5328 PflMI CCAn_nnn'nTGG Cuts at: 1108 2185 3894 1108 Size: 1077 1709 4280 Fragments arranged by size: 4280 1709 1077 PfoI T'CCnGG_A Cuts at: 2184 2745 2184 Size: 561 6505 PmeI GTTT'AAAC Cuts at: 4370 4370 Size: 7066 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4232 4264 5135 5167 6648 6680 4232 Size: 32 871 32 1481 32 4618 Fragments arranged by size: 4618 1481 871 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3025 3843 3025 Size: 818 6248 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7066 PsiI TTA'TAA Cuts at: 4636 4636 Size: 7066 PspOMI G'GGCC_C Cuts at: 2801 2801 Size: 7066 PstI C_TGCA'G Cuts at: 830 2211 2502 2673 2718 4153 4418 830 Size: 1381 291 171 45 1435 265 3478 Fragments arranged by size: 3478 1435 1381 291 265 171 45 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7066 PvuII CAG'CTG Cuts at: 2393 2578 2719 2785 2911 3523 4202 2393 Size: 185 141 66 126 612 679 5257 Fragments arranged by size: 5257 679 612 185 141 126 66 SacI G_AGCT'C Cuts at: 4320 4320 Size: 7066 SalI G'TCGA_C Cuts at: 1936 4408 1936 Size: 2472 4594 SbfI CC_TGCA'GG Cuts at: 4418 4418 Size: 7066 ScaI AGT'ACT Cuts at: 2516 5314 7026 2516 Size: 2798 1712 2556 Fragments arranged by size: 2798 2556 1712 SexAI A'CCwGG_T Cuts at: 2226 2226 Size: 7066 SfcI C'TryA_G Cuts at: 826 1045 2207 2498 2669 2714 2828 3646 4149 Size: 219 1162 291 171 45 114 818 503 Cuts at: 4149 4414 5549 6230 6421 826 Size: 265 1135 681 191 1471 Fragments arranged by size: 1471 1162 1135 818 681 503 291 265 219 191 171 114 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7066 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7066 SmaI CCC'GGG Cuts at: 1872 4393 1872 Size: 2521 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3152 3758 5170 6071 6333 820 Size: 197 926 1209 606 1412 901 262 1553 Fragments arranged by size: 1553 1412 1209 926 901 606 262 197 SnaBI TAC'GTA Cuts at: 493 4857 493 Size: 4364 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7066 SphI G_CATG'C Cuts at: 1132 4424 1132 Size: 3292 3774 SspI AAT'ATT Cuts at: 5 52 4990 5 Size: 47 4938 2081 Fragments arranged by size: 4938 2081 47 StuI AGG'CCT Cuts at: 1517 2642 1517 Size: 1125 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 2035 2310 3582 4505 513 Size: 552 939 31 275 1272 923 3074 Fragments arranged by size: 3074 1272 939 923 552 275 31 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2192 3954 5093 5110 5263 5448 5893 Size: 1255 612 1762 1139 17 153 185 445 Cuts at: 5893 325 Size: 1498 Fragments arranged by size: 1762 1498 1255 1139 612 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2343 2514 5312 Size: 274 80 33 51 1443 366 171 2798 Cuts at: 5312 7024 96 Size: 1712 138 Fragments arranged by size: 2798 1712 1443 366 274 171 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4045 4077 4328 4360 7026 7058 4045 Size: 32 251 32 2666 32 4053 Fragments arranged by size: 4053 2666 251 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3388 4250 1316 Size: 2072 862 4132 Fragments arranged by size: 4132 2072 862 XbaI T'CTAG_A Cuts at: 4402 4402 Size: 7066 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2182 2558 3350 2182 Size: 376 792 5898 Fragments arranged by size: 5898 792 376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7066 XmaI C'CCGG_G Cuts at: 1870 4391 1870 Size: 2521 4545 XmnI GAAnn'nnTTC Cuts at: 2046 3614 5195 2046 Size: 1568 1581 3917 Fragments arranged by size: 3917 1581 1568 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4874 276 Size: 53 83 186 936 3340 2468 Fragments arranged by size: 3340 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI