(Circular) (Six-base) MAPSORT of: pFN21AB3222.seq Check: 6322 from: 1 to: 7642 pFN21AB3222 7642 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5452 278 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4985 1937 Size: 3048 4594 Acc65I G'GTAC_C Cuts at: 2040 4963 2040 Size: 2923 4719 AclI AA'CG_TT Cuts at: 1563 3639 5769 6142 1563 Size: 2076 2130 373 3063 Fragments arranged by size: 3063 2130 2076 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3175 4490 4511 4646 5705 7089 7357 1632 Size: 1543 1315 21 135 1059 1384 268 1917 Fragments arranged by size: 1917 1543 1384 1315 1059 268 135 21 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7445 AflIII A'CryG_T Cuts at: 1203 2302 2588 6541 7518 1203 Size: 1099 286 3953 977 1327 Fragments arranged by size: 3953 1327 1099 977 286 AgeI A'CCGG_T Cuts at: 6473 6473 Size: 7642 AhdI GACnn_n'nnGTC Cuts at: 2734 6371 2734 Size: 3637 4005 AleI CACnn'nnGTG Cuts at: 1166 2669 3326 1166 Size: 1503 657 5482 Fragments arranged by size: 5482 1503 657 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7642 AlwNI CAG_nnn'CTG Cuts at: 4402 4744 4840 5385 6957 4402 Size: 342 96 545 1572 5087 Fragments arranged by size: 5087 1572 545 342 96 ApaLI G'TGCA_C Cuts at: 2660 3259 5699 6855 2660 Size: 599 2440 1156 3447 Fragments arranged by size: 3447 2440 1156 599 ApoI r'AATT_y Cuts at: 1493 2075 3704 4510 4951 5177 5329 7438 1493 Size: 582 1629 806 441 226 152 2109 1697 Fragments arranged by size: 2109 1697 1629 806 582 441 226 152 AseI AT'TA_AT Cuts at: 160 1032 6196 160 Size: 872 5164 1606 Fragments arranged by size: 5164 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 AvaI C'yCGr_G Cuts at: 1870 1943 2128 3029 3363 4967 1870 Size: 73 185 901 334 1604 4545 Fragments arranged by size: 4545 1604 901 334 185 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2354 2387 2354 Size: 33 7609 BamHI G'GATC_C Cuts at: 4972 5339 4972 Size: 367 7275 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2040 2930 4963 6418 618 Size: 325 217 627 253 890 2033 1455 1842 Fragments arranged by size: 2033 1842 1455 890 627 325 253 217 BanII G_rGCy'C Cuts at: 1391 2600 3411 4961 1391 Size: 1209 811 1550 4072 Fragments arranged by size: 4072 1550 1209 811 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2340 2466 3328 928 Size: 971 441 126 862 5242 Fragments arranged by size: 5242 971 862 441 126 BbvCI CC'TCA_GC Cuts at: 4385 4385 Size: 7642 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2289 2323 4585 4619 5833 5867 1903 Size: 34 352 34 2262 34 1214 34 3678 Fragments arranged by size: 3678 2262 1214 352 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5534 6744 5534 Size: 1210 6432 BclI T'GATC_A Cuts at: 1548 2829 1548 Size: 1281 6361 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6253 136 Size: 107 122 71 631 605 4581 1525 Fragments arranged by size: 4581 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3801 7637 3801 Size: 3836 3806 BlpI GC'TnA_GC Cuts at: 4754 5059 4754 Size: 305 7337 Bme1580I G_kGCm'C Cuts at: 1790 2664 3263 5703 6859 1790 Size: 874 599 2440 1156 2573 Fragments arranged by size: 2573 2440 1156 874 599 BmgBI CAC'GTC Cuts at: 1318 2589 3676 4695 1318 Size: 1271 1087 1019 4265 Fragments arranged by size: 4265 1271 1087 1019 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2740 5373 6331 Size: 416 53 83 399 24 1323 2633 958 Cuts at: 6331 442 Size: 1753 Fragments arranged by size: 2633 1753 1323 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7642 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3011 3787 3011 Size: 776 6866 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2075 2387 2872 3089 3842 4283 4717 Size: 827 9 312 485 217 753 441 434 Cuts at: 4717 6302 1239 Size: 1585 2579 Fragments arranged by size: 2579 1585 827 753 485 441 434 312 217 9 Bpu10I CC'TnA_GC Cuts at: 2999 4385 4911 2999 Size: 1386 526 5730 Fragments arranged by size: 5730 1386 526 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5767 6632 6930 5767 Size: 865 298 6479 Fragments arranged by size: 6479 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2241 3049 6305 882 Size: 617 742 808 3256 2219 Fragments arranged by size: 3256 2219 808 742 617 BsaAI yAC'GTr Cuts at: 493 1204 2303 2434 5433 493 Size: 711 1099 131 2999 2702 Fragments arranged by size: 2999 2702 1099 711 131 BsaBI GATnn'nnATC Cuts at: 3965 7636 3965 Size: 3671 3971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2735 5449 5831 6466 Size: 53 83 186 936 1202 2714 382 635 Cuts at: 6466 275 Size: 1451 Fragments arranged by size: 2714 1451 1202 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2535 6075 6473 6747 6894 7305 2535 Size: 3540 398 274 147 411 2872 Fragments arranged by size: 3540 2872 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 2612 2642 4025 Size: 30 165 30 786 30 393 30 1383 Cuts at: 4025 4055 1178 Size: 30 4765 Fragments arranged by size: 4765 1383 786 393 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2363 2827 3473 3935 4094 4097 4126 Size: 1024 153 464 646 462 159 3 29 Cuts at: 4126 1186 Size: 4702 Fragments arranged by size: 4702 1024 646 464 462 159 153 29 3 BseYI C'CCAG_C Cuts at: 3665 3878 4707 6845 7341 3665 Size: 213 829 2138 496 3966 Fragments arranged by size: 3966 2138 829 496 213 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2944 4136 1163 Size: 573 1208 1192 4669 Fragments arranged by size: 4669 1208 1192 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5092 5853 6881 1540 Size: 275 186 3091 761 1028 2301 Fragments arranged by size: 3091 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2535 2664 2946 3263 3384 4220 5703 5788 6859 Size: 129 282 317 121 836 1483 85 1071 Cuts at: 6859 2535 Size: 3318 Fragments arranged by size: 3318 1483 1071 836 317 282 129 121 85 BsmI GAATG_Cn' Cuts at: 2473 2647 5153 5246 2473 Size: 174 2506 93 4869 Fragments arranged by size: 4869 2506 174 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2525 3666 2525 Size: 1141 6501 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2535 2600 2664 2946 3263 3384 3411 Size: 399 745 65 64 282 317 121 27 Cuts at: 3411 4220 4961 5703 5788 6859 1391 Size: 809 741 742 85 1071 2174 Fragments arranged by size: 2174 1071 809 745 742 741 399 317 282 121 85 65 64 27 BspEI T'CCGG_A Cuts at: 7305 7305 Size: 7642 BspHI T'CATG_A Cuts at: 2832 4791 5531 7261 2832 Size: 1959 740 1730 3213 Fragments arranged by size: 3213 1959 1730 740 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3615 4596 4997 844 Size: 2771 981 401 3489 Fragments arranged by size: 3489 2771 981 401 BsrBI CCG'CTC Cuts at: 1394 1823 2956 2983 5529 1394 Size: 429 1133 27 2546 3507 Fragments arranged by size: 3507 2546 1133 429 27 BsrDI GCAATG_nn' Cuts at: 65 1248 6137 6311 65 Size: 1183 4889 174 1396 Fragments arranged by size: 4889 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6286 6473 1700 Size: 110 4476 187 2869 Fragments arranged by size: 4476 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7642 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7642 BssSI C'ACGA_G Cuts at: 3052 5702 6714 3052 Size: 2650 1012 3980 Fragments arranged by size: 3980 2650 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2025 3085 794 Size: 1231 1060 5351 Fragments arranged by size: 5351 1231 1060 BstBI TT'CG_AA Cuts at: 2309 3708 6446 2309 Size: 1399 2738 3505 Fragments arranged by size: 3505 2738 1399 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7642 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2044 2617 3886 3985 4307 2011 Size: 33 573 1269 99 322 5346 Fragments arranged by size: 5346 1269 573 322 99 33 BstYI r'GATC_y Cuts at: 1972 2161 2223 3111 3801 3870 4711 4972 5339 Size: 189 62 888 690 69 841 261 367 Cuts at: 5339 5724 5741 7193 7279 7291 7637 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 888 841 690 385 367 346 261 189 86 69 62 17 12 Bsu36I CC'TnA_GG Cuts at: 3470 3512 3470 Size: 42 7600 BtgI C'CryG_G Cuts at: 513 1065 2211 2667 3324 3942 513 Size: 552 1146 456 657 618 4213 Fragments arranged by size: 4213 1146 657 618 552 456 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6903 Fragments arranged by size: 6903 683 56 BtsI GCAGTG_nn' Cuts at: 4124 4699 5158 5952 5972 4124 Size: 575 459 794 20 5794 Fragments arranged by size: 5794 794 575 459 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7642 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7607 DraI TTT'AAA Cuts at: 4946 4946 Size: 7642 DraIII CAC_nnn'GTG Cuts at: 2668 3228 2668 Size: 560 7082 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3680 4787 6649 809 Size: 507 2364 1107 1862 1802 Fragments arranged by size: 2364 1862 1802 1107 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2214 2568 Size: 54 1425 50 275 18 204 180 354 Cuts at: 2568 2709 5089 5978 7607 8 Size: 141 2380 889 1629 43 Fragments arranged by size: 2380 1629 1425 889 354 275 204 180 141 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5089 1537 Size: 275 3277 4090 Fragments arranged by size: 4090 3277 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2340 2903 3224 3609 4071 5572 1353 Size: 987 563 321 385 462 1501 3423 Fragments arranged by size: 3423 1501 987 563 462 385 321 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3509 6229 6603 6749 387 Size: 1064 138 1920 2720 374 146 1280 Fragments arranged by size: 2720 1920 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2075 2387 2872 3089 3175 3842 Size: 393 434 9 312 485 217 86 667 Cuts at: 3842 4283 4490 4511 4646 4717 5705 6302 7089 Size: 441 207 21 135 71 988 597 787 Cuts at: 7089 7357 1239 Size: 268 1524 Fragments arranged by size: 1524 988 787 667 597 485 441 434 393 312 268 217 207 135 86 71 21 9 EcoNI CCTnn'n_nnAGG Cuts at: 3455 3455 Size: 7642 EcoO109I rG'GnC_Cy Cuts at: 2050 2824 3214 3768 4198 4267 2050 Size: 774 390 554 430 69 5425 Fragments arranged by size: 5425 774 554 430 390 69 EcoRI G'AATT_C Cuts at: 4510 4951 5329 4510 Size: 441 378 6823 Fragments arranged by size: 6823 441 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2104 2104 Size: 7642 FspI TGC'GCA Cuts at: 6148 6148 Size: 7642 HaeII r_GCGC'y Cuts at: 1678 4342 6789 1678 Size: 2664 2447 2531 Fragments arranged by size: 2664 2531 2447 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2505 2537 2720 2752 4025 Size: 32 135 32 933 32 183 32 1273 Cuts at: 4025 4057 6283 6315 6357 6389 1373 Size: 32 2226 32 42 32 2626 Fragments arranged by size: 2626 2226 1273 933 183 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4986 5232 669 Size: 988 281 3048 246 3079 Fragments arranged by size: 3079 3048 988 281 246 HindIII A'AGCT_T Cuts at: 748 3659 4658 748 Size: 2911 999 3732 Fragments arranged by size: 3732 2911 999 HpaI GTT'AAC Cuts at: 5232 5232 Size: 7642 KpnI G_GTAC'C Cuts at: 2044 4967 2044 Size: 2923 4719 MfeI C'AATT_G Cuts at: 5241 5241 Size: 7642 MluI A'CGCG_T Cuts at: 7518 7518 Size: 7642 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2706 3489 4650 6756 6940 1305 Size: 1401 783 1161 2106 184 2007 Fragments arranged by size: 2106 2007 1401 1161 783 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2036 2570 2711 10 Size: 54 1425 343 204 534 141 4941 Fragments arranged by size: 4941 1425 534 343 204 141 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2174 2216 2615 2669 3326 3569 Size: 648 843 165 42 399 54 657 243 Cuts at: 3569 3983 5600 5959 6118 518 Size: 414 1617 359 159 2042 Fragments arranged by size: 2042 1617 843 657 648 414 399 359 243 165 159 54 42 MspA1I CmG'CkG Cuts at: 1606 2025 3326 3886 5058 5735 6883 7128 1606 Size: 419 1301 560 1172 677 1148 245 2120 Fragments arranged by size: 2120 1301 1172 1148 677 560 419 245 NcoI C'CATG_G Cuts at: 513 1065 2211 3942 513 Size: 552 1146 1731 4213 Fragments arranged by size: 4213 1731 1146 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7642 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7642 NotI GC'GGCC_GC Cuts at: 5089 5089 Size: 7642 NspI r_CATG'y Cuts at: 1132 1229 3107 4066 5000 6545 1132 Size: 97 1878 959 934 1545 2229 Fragments arranged by size: 2229 1878 1545 959 934 97 PasI CC'CwG_GG Cuts at: 1348 4045 4271 1348 Size: 2697 226 4719 Fragments arranged by size: 4719 2697 226 PciI A'CATG_T Cuts at: 6541 6541 Size: 7642 PflMI CCAn_nnn'nTGG Cuts at: 1108 4402 1108 Size: 3294 4348 PfoI T'CCnGG_A Cuts at: 3579 3579 Size: 7642 PmeI GTTT'AAAC Cuts at: 4946 4946 Size: 7642 PmlI CAC'GTG Cuts at: 2303 2303 Size: 7642 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5711 5743 7224 7256 5711 Size: 32 1481 32 6097 Fragments arranged by size: 6097 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2050 2824 3214 3768 4267 2050 Size: 774 390 554 499 5425 Fragments arranged by size: 5425 774 554 499 390 PshAI GACnn'nnGTC Cuts at: 1613 4922 1613 Size: 3309 4333 PsiI TTA'TAA Cuts at: 5212 5212 Size: 7642 PstI C_TGCA'G Cuts at: 830 3081 3360 3427 3489 3870 4836 4994 830 Size: 2251 279 67 62 381 966 158 3478 Fragments arranged by size: 3478 2251 966 381 279 158 67 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7642 PvuII CAG'CTG Cuts at: 3886 3886 Size: 7642 SacII CC_GC'GG Cuts at: 3327 3327 Size: 7642 SalI G'TCGA_C Cuts at: 1936 4984 1936 Size: 3048 4594 SanDI GG'GwC_CC Cuts at: 4267 4267 Size: 7642 SbfI CC_TGCA'GG Cuts at: 4994 4994 Size: 7642 ScaI AGT'ACT Cuts at: 3146 3158 5890 7602 3146 Size: 12 2732 1712 3186 Fragments arranged by size: 3186 2732 1712 12 SfcI C'TryA_G Cuts at: 826 1045 3077 3356 3423 3485 3556 3866 4832 Size: 219 2032 279 67 62 71 310 966 Cuts at: 4832 4990 6125 6806 6997 826 Size: 158 1135 681 191 1471 Fragments arranged by size: 2032 1471 1135 966 681 310 279 219 191 158 71 67 62 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7642 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7642 SmaI CCC'GGG Cuts at: 1872 3365 4969 1872 Size: 1493 1604 4545 Fragments arranged by size: 4545 1604 1493 SmlI C'TyrA_G Cuts at: 820 1017 1943 5746 6647 6909 820 Size: 197 926 3803 901 262 1553 Fragments arranged by size: 3803 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5433 493 Size: 4940 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7642 SphI G_CATG'C Cuts at: 1132 5000 1132 Size: 3868 3774 SspI AAT'ATT Cuts at: 5 52 5566 5 Size: 47 5514 2081 Fragments arranged by size: 5514 2081 47 StuI AGG'CCT Cuts at: 1517 3035 1517 Size: 1518 6124 StyI C'CwwG_G Cuts at: 513 1065 2211 3942 4068 4377 5081 513 Size: 552 1146 1731 126 309 704 3074 Fragments arranged by size: 3074 1731 1146 704 552 309 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2468 2875 5669 5686 5839 6024 6469 Size: 1255 888 407 2794 17 153 185 445 Cuts at: 6469 325 Size: 1498 Fragments arranged by size: 2794 1498 1255 888 445 407 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2421 3144 3156 Size: 274 80 33 51 1443 444 723 12 Cuts at: 3156 3600 5888 7600 96 Size: 444 2288 1712 138 Fragments arranged by size: 2288 1712 1443 723 444 444 274 138 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2189 2221 4166 4198 7602 7634 2189 Size: 32 1945 32 3404 32 2197 Fragments arranged by size: 3404 2197 1945 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2517 1316 Size: 1201 6441 XbaI T'CTAG_A Cuts at: 4978 4978 Size: 7642 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3058 3058 Size: 7642 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7642 XmaI C'CCGG_G Cuts at: 1870 3363 4967 1870 Size: 1493 1604 4545 Fragments arranged by size: 4545 1604 1493 XmnI GAAnn'nnTTC Cuts at: 3524 4893 5771 3524 Size: 1369 878 5395 Fragments arranged by size: 5395 1369 878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5450 276 Size: 53 83 186 936 3916 2468 Fragments arranged by size: 3916 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI