################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BcgI BclI BcnI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1407I BspEI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI Bsu36I CfoI Cfr10I CpoI DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I SspBI StuI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AclI 2058 AACGTT AA^CGTT AcsI 214 GAATTC [GA]^AATT[CT] AfaI 1180 GTAC GT^AC AflII 1066 CTTAAG C^TTAAG AflIII 1623 ACATGT A^C[GA][CT]GT Alw44I 581 GTGCAC G^TGCAC ApaLI 581 GTGCAC G^TGCAC ApoI 214 GAATTC [GA]^AATT[CT] BanI 627 GGTGCC G^G[CT][GA]CC BbsI 2638 GAAGAC GAAGAC(2/6) BfrI 1066 CTTAAG C^TTAAG BglII 1316 AGATCT A^GATCT BpuAI 2638 GAAGAC GAAGAC(2/6) BspDI 1915 ATCGAT AT^CGAT BspHI 309 TCATGA T^CATGA BspLU11I 1623 ACATGT A^CATGT BspMI 1176 GCAGGT ACCTGC(4/8) Bst1107I 574 GTATAC GTA^TAC BstXI 563 CCATGTTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 574 GTATAC GTA^TAC ClaI 1915 ATCGAT AT^CGAT DraI 2398 TTTAAA TTT^AAA DraII 1061 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 361 CCATGG C^C[GA][CT]GG EcoO109I 1061 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 214 GAATTC G^AATTC HgaI 231 GCGTC GACGC(5/10) HindIII 826 AAGCTT A^AGCTT NcoI 361 CCATGG C^CATGG PpuMI 1061 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 2058 AACGTT AA^CGTT RcaI 309 TCATGA T^CATGA RsaI 1180 GTAC GT^AC SpeI 1300 ACTAGT A^CTAGT XcmI 2369 CCATCAACAAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AciI 7 GCGG CCGC(-3/-1) 10 GCGG Asp700I 916 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1426 GAACCTCTTC BbvCI 1584 CCTCAGC CCTCAGC(-5/-2) 1258 GCTGAGG BciVI 197 GTATCC GTATCC(6/5) 658 GTATCC BlpI 352 GCTGAGC GC^T[ACGT]AGC 1359 GCTGAGC Bpu1102I 352 GCTGAGC GC^T[ACGT]AGC 1359 GCTGAGC BsgI 1533 GTGCAG GTGCAG(16/14) 2024 GTGCAG BsmI 533 GAATGC GAATGC(1/-1) 1550 GCATTC BstF5I 1203 GGATG GGATG(2/0) 1896 GGATG Cac8I 2107 GCAAGC GC[ACGT]^[ACGT]GC 2610 GCTTGC CelII 352 GCTGAGC GC^T[ACGT]AGC 1359 GCTGAGC EcoNI 1936 CCTTTCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2422 CCTTTCCCAGG EcoRV 487 GATATC GAT^ATC 2676 GATATC FokI 1203 GGATG GGATG(9/13) 1896 GGATG HincII 1027 GTTGAC GT[CT]^[GA]AC 2197 GTCAAC HindII 1027 GTTGAC GT[CT]^[GA]AC 2197 GTCAAC NspI 241 GCATGT [GA]CATG^[CT] 1623 ACATGT PflMI 421 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1921 CCATTTGGTGG PstI 347 CTGCAG CTGCA^G 665 CTGCAG SacI 355 GAGCTC GAGCT^C 2232 GAGCTC SapI 1354 GAAGAGC GCTCTTC(1/4) 2599 GAAGAGC Tsp45I 1711 GTGAC ^GT[GC]AC 1729 GTGAC Van91I 421 CCAGTGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1921 CCATTTGGTGG XmnI 916 GAAATGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1426 GAACCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccI AluI AlwI AspHI AvaII BanII BbvI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstNI BstYI Cfr13I DdeI DpnII EarI EcoRII EcoT14I Fnu4HI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PleI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII