(Circular) (Six-base) MAPSORT of: pFN21AB3218.seq Check: 2830 from: 1 to: 7393 pFN21AB3218 7393 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5203 278 Size: 53 83 186 936 3667 2468 Fragments arranged by size: 3667 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2580 3373 3813 4736 1937 Size: 643 793 440 923 4594 Fragments arranged by size: 4594 923 793 643 440 Acc65I G'GTAC_C Cuts at: 4714 4714 Size: 7393 AclI AA'CG_TT Cuts at: 1563 4064 5520 5893 1563 Size: 2501 1456 373 3063 Fragments arranged by size: 3063 2501 1456 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2403 3422 3964 4168 4525 4578 5456 6840 Size: 771 1019 542 204 357 53 878 1384 Cuts at: 6840 7108 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1019 878 771 542 357 268 204 53 AflII C'TTAA_G Cuts at: 820 1017 3071 820 Size: 197 2054 5142 Fragments arranged by size: 5142 2054 197 AflIII A'CryG_T Cuts at: 1203 3628 6292 7269 1203 Size: 2425 2664 977 1327 Fragments arranged by size: 2664 2425 1327 977 AgeI A'CCGG_T Cuts at: 6224 6224 Size: 7393 AhdI GACnn_n'nnGTC Cuts at: 6122 6122 Size: 7393 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7393 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4632 1184 Size: 3448 3945 AlwNI CAG_nnn'CTG Cuts at: 5136 6708 5136 Size: 1572 5821 ApaLI G'TGCA_C Cuts at: 2586 5450 6606 2586 Size: 2864 1156 3373 Fragments arranged by size: 3373 2864 1156 ApoI r'AATT_y Cuts at: 1493 2219 4702 4928 5080 7189 1493 Size: 726 2483 226 152 2109 1697 Fragments arranged by size: 2483 2109 1697 726 226 152 AseI AT'TA_AT Cuts at: 160 1032 5947 160 Size: 872 4915 1606 Fragments arranged by size: 4915 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7393 AvaI C'yCGr_G Cuts at: 1870 1943 4718 1870 Size: 73 2775 4545 Fragments arranged by size: 4545 2775 73 BamHI G'GATC_C Cuts at: 4723 5090 4723 Size: 367 7026 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2632 4714 6169 618 Size: 325 217 627 845 2082 1455 1842 Fragments arranged by size: 2082 1842 1455 845 627 325 217 BanII G_rGCy'C Cuts at: 1391 2364 3663 4241 4712 1391 Size: 973 1299 578 471 4072 Fragments arranged by size: 4072 1299 973 578 471 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4650 928 Size: 971 2751 3671 Fragments arranged by size: 3671 2751 971 BbvCI CC'TCA_GC Cuts at: 3264 3590 3264 Size: 326 7067 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5584 5618 1903 Size: 34 3647 34 3678 Fragments arranged by size: 3678 3647 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2213 2674 5285 6495 2213 Size: 461 2611 1210 3111 Fragments arranged by size: 3111 2611 1210 461 BclI T'GATC_A Cuts at: 1548 1548 Size: 7393 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6004 136 Size: 107 122 71 631 605 4332 1525 Fragments arranged by size: 4332 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3321 7388 3321 Size: 4067 3326 BlpI GC'TnA_GC Cuts at: 2358 3365 4810 2358 Size: 1007 1445 4941 Fragments arranged by size: 4941 1445 1007 Bme1580I G_kGCm'C Cuts at: 1790 2254 2590 5454 6610 1790 Size: 464 336 2864 1156 2573 Fragments arranged by size: 2864 2573 1156 464 336 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7393 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2420 5124 6082 Size: 416 53 83 399 24 1003 2704 958 Cuts at: 6082 442 Size: 1753 Fragments arranged by size: 2704 1753 1003 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7393 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2607 2612 3248 3663 6053 1239 Size: 1368 5 636 415 2390 2579 Fragments arranged by size: 2579 2390 1368 636 415 5 Bpu10I CC'TnA_GC Cuts at: 3264 3590 3264 Size: 326 7067 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3224 3802 5518 6383 6681 3224 Size: 578 1716 865 298 3936 Fragments arranged by size: 3936 1716 865 578 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6056 882 Size: 617 4557 2219 Fragments arranged by size: 4557 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5184 493 Size: 711 3980 2702 Fragments arranged by size: 3980 2702 711 BsaBI GATnn'nnATC Cuts at: 7387 7387 Size: 7393 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5200 5582 6217 275 Size: 53 83 186 936 3667 382 635 1451 Fragments arranged by size: 3667 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5826 6224 6498 6645 7056 5826 Size: 398 274 147 411 6163 Fragments arranged by size: 6163 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3177 3207 1178 Size: 30 165 30 1774 30 5364 Fragments arranged by size: 5364 1774 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2892 3245 3281 3413 1186 Size: 1706 353 36 132 5166 Fragments arranged by size: 5166 1706 353 132 36 BseYI C'CCAG_C Cuts at: 2019 3222 4309 6596 7092 2019 Size: 1203 1087 2287 496 2320 Fragments arranged by size: 2320 2287 1203 1087 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3559 4050 1163 Size: 573 1823 491 4506 Fragments arranged by size: 4506 1823 573 491 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4843 5604 6632 1540 Size: 275 186 2842 761 1028 2301 Fragments arranged by size: 2842 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 2590 4241 5454 5539 6610 2364 Size: 226 1651 1213 85 1071 3147 Fragments arranged by size: 3147 1651 1213 1071 226 85 BsmI GAATG_Cn' Cuts at: 2544 3555 4904 4997 2544 Size: 1011 1349 93 4940 Fragments arranged by size: 4940 1349 1011 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2254 2364 2590 3663 4241 4712 5454 Size: 399 464 110 226 1073 578 471 742 Cuts at: 5454 5539 6610 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1073 1071 742 578 471 464 399 226 110 85 BspEI T'CCGG_A Cuts at: 7056 7056 Size: 7393 BspHI T'CATG_A Cuts at: 2314 5282 7012 2314 Size: 2968 1730 2695 Fragments arranged by size: 2968 2695 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3172 4748 844 Size: 2328 1576 3489 Fragments arranged by size: 3489 2328 1576 BsrBI CCG'CTC Cuts at: 1394 1823 5280 1394 Size: 429 3457 3507 Fragments arranged by size: 3507 3457 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5888 6062 65 Size: 1183 4640 174 1396 Fragments arranged by size: 4640 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6037 6224 1700 Size: 110 4227 187 2869 Fragments arranged by size: 4227 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7393 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7393 BssSI C'ACGA_G Cuts at: 5453 6465 5453 Size: 1012 6381 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7393 BstBI TT'CG_AA Cuts at: 6197 6197 Size: 7393 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7393 BstXI CCAn_nnnn'nTGG Cuts at: 2575 2575 Size: 7393 BstYI r'GATC_y Cuts at: 1972 3118 3321 3799 3955 4213 4370 4459 4723 Size: 1146 203 478 156 258 157 89 264 Cuts at: 4723 5090 5475 5492 6944 7030 7042 7388 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1146 478 385 367 346 264 258 203 157 156 89 86 17 12 BstZ17I GTA'TAC Cuts at: 2581 2581 Size: 7393 BtgI C'CryG_G Cuts at: 513 1065 2004 2366 513 Size: 552 939 362 5540 Fragments arranged by size: 5540 939 552 362 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6654 Fragments arranged by size: 6654 683 56 BtsI GCAGTG_nn' Cuts at: 3610 3892 4313 4909 5703 5723 3610 Size: 282 421 596 794 20 5280 Fragments arranged by size: 5280 794 596 421 282 20 ClaI AT'CG_AT Cuts at: 1554 3921 1554 Size: 2367 5026 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7358 DraI TTT'AAA Cuts at: 4405 4697 4405 Size: 292 7101 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6400 809 Size: 507 5084 1802 Fragments arranged by size: 5084 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4840 5729 7358 Size: 54 1425 50 275 18 3010 889 1629 Cuts at: 7358 8 Size: 43 Fragments arranged by size: 3010 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4840 1537 Size: 275 3028 4090 Fragments arranged by size: 4090 3028 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2497 3289 3354 3441 4599 5323 1353 Size: 1144 792 65 87 1158 724 3423 Fragments arranged by size: 3423 1158 1144 792 724 87 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5980 6354 6500 387 Size: 1064 138 4391 374 146 1280 Fragments arranged by size: 4391 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2362 4239 2362 Size: 1877 5516 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2403 2607 2612 3248 3422 3663 3964 Size: 393 771 204 5 636 174 241 301 Cuts at: 3964 4168 4525 4578 5456 6053 6840 7108 1239 Size: 204 357 53 878 597 787 268 1524 Fragments arranged by size: 1524 878 787 771 636 597 393 357 301 268 241 204 204 174 53 5 EcoNI CCTnn'n_nnAGG Cuts at: 3945 4431 3945 Size: 486 6907 EcoO109I rG'GnC_Cy Cuts at: 3067 3067 Size: 7393 EcoRI G'AATT_C Cuts at: 2219 4702 5080 2219 Size: 2483 378 4532 Fragments arranged by size: 4532 2483 378 EcoRV GAT'ATC Cuts at: 2494 4683 2494 Size: 2189 5204 FspI TGC'GCA Cuts at: 5899 5899 Size: 7393 HaeII r_GCGC'y Cuts at: 1678 6540 1678 Size: 4862 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2730 2762 3271 3303 4268 Size: 32 135 32 1158 32 509 32 965 Cuts at: 4268 4300 4318 4350 6034 6066 6108 6140 1373 Size: 32 18 32 1684 32 42 32 2626 Fragments arranged by size: 2626 1684 1158 965 509 135 42 32 32 32 32 32 32 32 32 18 HincII GTy'rAC Cuts at: 669 1657 1938 3034 4204 4737 4983 669 Size: 988 281 1096 1170 533 246 3079 Fragments arranged by size: 3079 1170 1096 988 533 281 246 HindIII A'AGCT_T Cuts at: 748 2831 748 Size: 2083 5310 HpaI GTT'AAC Cuts at: 4983 4983 Size: 7393 KpnI G_GTAC'C Cuts at: 4718 4718 Size: 7393 MfeI C'AATT_G Cuts at: 4992 4992 Size: 7393 MluI A'CGCG_T Cuts at: 7269 7269 Size: 7393 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3408 4361 6507 6691 1305 Size: 2103 953 2146 184 2007 Fragments arranged by size: 2146 2103 2007 953 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5571 Fragments arranged by size: 5571 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5351 5710 5869 518 Size: 648 4185 359 159 2042 Fragments arranged by size: 4185 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2357 3226 3743 4809 5486 6634 6879 1606 Size: 751 869 517 1066 677 1148 245 2120 Fragments arranged by size: 2120 1148 1066 869 751 677 517 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2366 513 Size: 552 939 362 5540 Fragments arranged by size: 5540 939 552 362 NdeI CA'TA_TG Cuts at: 387 387 Size: 7393 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7393 NotI GC'GGCC_GC Cuts at: 4840 4840 Size: 7393 NspI r_CATG'y Cuts at: 1132 1229 2250 3632 4751 6296 1132 Size: 97 1021 1382 1119 1545 2229 Fragments arranged by size: 2229 1545 1382 1119 1021 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7393 PciI A'CATG_T Cuts at: 3628 6292 3628 Size: 2664 4729 PflMI CCAn_nnn'nTGG Cuts at: 1108 2432 3932 1108 Size: 1324 1500 4569 Fragments arranged by size: 4569 1500 1324 PmeI GTTT'AAAC Cuts at: 4697 4697 Size: 7393 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5462 5494 6975 7007 5462 Size: 32 1481 32 5848 Fragments arranged by size: 5848 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3067 3067 Size: 7393 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7393 PsiI TTA'TAA Cuts at: 4963 4963 Size: 7393 PstI C_TGCA'G Cuts at: 830 2356 2674 4745 830 Size: 1526 318 2071 3478 Fragments arranged by size: 3478 2071 1526 318 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7393 PvuII CAG'CTG Cuts at: 2357 3226 3743 2357 Size: 869 517 6007 Fragments arranged by size: 6007 869 517 SacI G_AGCT'C Cuts at: 2364 4241 2364 Size: 1877 5516 SalI G'TCGA_C Cuts at: 1936 4735 1936 Size: 2799 4594 SapI GCTCTTCn'nnn_ Cuts at: 3354 4599 3354 Size: 1245 6148 SbfI CC_TGCA'GG Cuts at: 4745 4745 Size: 7393 ScaI AGT'ACT Cuts at: 5641 7353 5641 Size: 1712 5681 SfcI C'TryA_G Cuts at: 826 1045 2071 2352 2670 3038 3164 3374 3573 Size: 219 1026 281 318 368 126 210 199 Cuts at: 3573 3738 4741 5876 6557 6748 826 Size: 165 1003 1135 681 191 1471 Fragments arranged by size: 1471 1135 1026 1003 681 368 318 281 219 210 199 191 165 126 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7393 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7393 SmaI CCC'GGG Cuts at: 1872 4720 1872 Size: 2848 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3071 3239 3817 5497 6398 6660 Size: 197 926 1128 168 578 1680 901 262 Cuts at: 6660 820 Size: 1553 Fragments arranged by size: 1680 1553 1128 926 901 578 262 197 168 SnaBI TAC'GTA Cuts at: 493 5184 493 Size: 4691 2702 SpeI A'CTAG_T Cuts at: 152 3305 152 Size: 3153 4240 SphI G_CATG'C Cuts at: 1132 4751 1132 Size: 3619 3774 SspI AAT'ATT Cuts at: 5 52 2409 2757 4470 5317 5 Size: 47 2357 348 1713 847 2081 Fragments arranged by size: 2357 2081 1713 847 348 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7393 StyI C'CwwG_G Cuts at: 513 1065 2004 2366 2497 2628 2853 4832 513 Size: 552 939 362 131 131 225 1979 3074 Fragments arranged by size: 3074 1979 939 552 362 225 131 131 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4276 5420 5437 5590 5775 6220 325 Size: 1255 2696 1144 17 153 185 445 1498 Fragments arranged by size: 2696 1498 1255 1144 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5639 7351 96 Size: 274 80 33 51 1443 3662 1712 138 Fragments arranged by size: 3662 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7353 7385 7353 Size: 32 7361 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7393 XbaI T'CTAG_A Cuts at: 4729 4729 Size: 7393 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4381 4381 Size: 7393 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7393 XmaI C'CCGG_G Cuts at: 1870 4718 1870 Size: 2848 4545 XmnI GAAnn'nnTTC Cuts at: 2925 3435 5522 2925 Size: 510 2087 4796 Fragments arranged by size: 4796 2087 510 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5201 276 Size: 53 83 186 936 3667 2468 Fragments arranged by size: 3667 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI