(Circular) (Six-base) MAPSORT of: pFN21AB3089.seq Check: 3802 from: 1 to: 6130 pFN21AB3089 6130 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3388 3388 Size: 6130 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2628 3940 278 Size: 53 83 186 936 1092 1312 2468 Fragments arranged by size: 2468 1312 1092 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2622 3473 1937 Size: 685 851 4594 Fragments arranged by size: 4594 851 685 Acc65I G'GTAC_C Cuts at: 3451 3451 Size: 6130 AclI AA'CG_TT Cuts at: 1563 4257 4630 1563 Size: 2694 373 3063 Fragments arranged by size: 3063 2694 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2163 3014 3168 4193 5577 5845 1632 Size: 531 851 154 1025 1384 268 1917 Fragments arranged by size: 1917 1384 1025 851 531 268 154 AfeI AGC'GCT Cuts at: 2813 2813 Size: 6130 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5933 AflIII A'CryG_T Cuts at: 1203 5029 6006 1203 Size: 3826 977 1327 Fragments arranged by size: 3826 1327 977 AgeI A'CCGG_T Cuts at: 4961 4961 Size: 6130 AhdI GACnn_n'nnGTC Cuts at: 2344 4859 2344 Size: 2515 3615 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6130 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2036 1184 Size: 852 5278 AlwNI CAG_nnn'CTG Cuts at: 2056 3873 5445 2056 Size: 1817 1572 2741 Fragments arranged by size: 2741 1817 1572 ApaLI G'TGCA_C Cuts at: 4187 5343 4187 Size: 1156 4974 ApoI r'AATT_y Cuts at: 1493 2123 3439 3665 3817 5926 1493 Size: 630 1316 226 152 2109 1697 Fragments arranged by size: 2109 1697 1316 630 226 152 AseI AT'TA_AT Cuts at: 160 1032 4684 160 Size: 872 3652 1606 Fragments arranged by size: 3652 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 AvaI C'yCGr_G Cuts at: 1870 1943 2587 3455 1870 Size: 73 644 868 4545 Fragments arranged by size: 4545 868 644 73 BamHI G'GATC_C Cuts at: 3460 3827 3460 Size: 367 5763 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2278 3107 3376 3451 4906 Size: 325 217 627 491 829 269 75 1455 Cuts at: 4906 618 Size: 1842 Fragments arranged by size: 1842 1455 829 627 491 325 269 217 75 BanII G_rGCy'C Cuts at: 1391 2356 2491 2907 3449 1391 Size: 965 135 416 542 4072 Fragments arranged by size: 4072 965 542 416 135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2151 3002 3016 928 Size: 971 252 851 14 4042 Fragments arranged by size: 4042 971 851 252 14 BbvCI CC'TCA_GC Cuts at: 2154 2154 Size: 6130 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4321 4355 1903 Size: 34 2384 34 3678 Fragments arranged by size: 3678 2384 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2468 4022 5232 2468 Size: 1554 1210 3366 Fragments arranged by size: 3366 1554 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6130 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4741 136 Size: 107 122 71 631 605 3069 1525 Fragments arranged by size: 3069 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6125 6125 Size: 6130 BlpI GC'TnA_GC Cuts at: 3547 3547 Size: 6130 Bme1580I G_kGCm'C Cuts at: 1790 4191 5347 1790 Size: 2401 1156 2573 Fragments arranged by size: 2573 2401 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6130 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2363 3861 4819 Size: 416 53 83 399 24 946 1498 958 Cuts at: 4819 442 Size: 1753 Fragments arranged by size: 1753 1498 958 946 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6130 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3227 3227 Size: 6130 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2033 4790 1239 Size: 794 2757 2579 Fragments arranged by size: 2757 2579 794 Bpu10I CC'TnA_GC Cuts at: 2154 2355 2154 Size: 201 5929 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2932 4255 5120 5418 2932 Size: 1323 865 298 3644 Fragments arranged by size: 3644 1323 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4793 882 Size: 617 3294 2219 Fragments arranged by size: 3294 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3921 493 Size: 711 2717 2702 Fragments arranged by size: 2717 2702 711 BsaBI GATnn'nnATC Cuts at: 6124 6124 Size: 6130 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2625 3937 4319 4954 Size: 53 83 186 936 1092 1312 382 635 Cuts at: 4954 275 Size: 1451 Fragments arranged by size: 1451 1312 1092 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4563 4961 5235 5382 5793 4563 Size: 398 274 147 411 4900 Fragments arranged by size: 4900 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2423 2453 2798 2828 3338 Size: 30 165 30 1020 30 345 30 510 Cuts at: 3338 3368 1178 Size: 30 3940 Fragments arranged by size: 3940 1020 510 345 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2447 2450 1186 Size: 1261 3 4866 Fragments arranged by size: 4866 1261 3 BseYI C'CCAG_C Cuts at: 5333 5829 5333 Size: 496 5634 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2896 1163 Size: 573 1160 4397 Fragments arranged by size: 4397 1160 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3580 4341 5369 1540 Size: 275 186 1579 761 1028 2301 Fragments arranged by size: 2301 1579 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2804 4191 4276 5347 2804 Size: 1387 85 1071 3587 Fragments arranged by size: 3587 1387 1071 85 BsmI GAATG_Cn' Cuts at: 3641 3734 3641 Size: 93 6037 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2356 2491 2804 2907 3449 4191 4276 Size: 399 566 135 313 103 542 742 85 Cuts at: 4276 5347 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 566 542 399 313 135 103 85 BspEI T'CCGG_A Cuts at: 5793 5793 Size: 6130 BspHI T'CATG_A Cuts at: 4019 5749 4019 Size: 1730 4400 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3388 3485 844 Size: 2544 97 3489 Fragments arranged by size: 3489 2544 97 BsrBI CCG'CTC Cuts at: 1394 1823 4017 1394 Size: 429 2194 3507 Fragments arranged by size: 3507 2194 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2502 4625 4799 65 Size: 1183 1254 2123 174 1396 Fragments arranged by size: 2123 1396 1254 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4774 4961 1700 Size: 110 2964 187 2869 Fragments arranged by size: 2964 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2301 96 Size: 2205 3925 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6130 BssSI C'ACGA_G Cuts at: 2024 2807 4190 5202 2024 Size: 783 1383 1012 2952 Fragments arranged by size: 2952 1383 1012 783 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6130 BstBI TT'CG_AA Cuts at: 4934 4934 Size: 6130 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6130 BstXI CCAn_nnnn'nTGG Cuts at: 2395 2498 2395 Size: 103 6027 BstYI r'GATC_y Cuts at: 1972 3460 3827 4212 4229 5681 5767 5779 6125 Size: 1488 367 385 17 1452 86 12 346 Cuts at: 6125 1972 Size: 1977 Fragments arranged by size: 1977 1488 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2132 2132 Size: 6130 BtgI C'CryG_G Cuts at: 513 1065 2004 2068 2193 2242 2376 3111 513 Size: 552 939 64 125 49 134 735 3532 Fragments arranged by size: 3532 939 735 552 134 125 64 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5391 Fragments arranged by size: 5391 683 56 BtsI GCAGTG_nn' Cuts at: 3646 4440 4460 3646 Size: 794 20 5316 Fragments arranged by size: 5316 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6130 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2271 2306 529 Size: 35 1707 35 4353 Fragments arranged by size: 4353 1707 35 35 DraI TTT'AAA Cuts at: 3434 3434 Size: 6130 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2324 5137 809 Size: 507 1008 2813 1802 Fragments arranged by size: 2813 1802 1008 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2079 2292 Size: 54 1425 50 275 18 207 42 213 Cuts at: 2292 3114 3577 4466 6095 8 Size: 822 463 889 1629 43 Fragments arranged by size: 1629 1425 889 822 463 275 213 207 54 50 43 42 18 EagI C'GGCC_G Cuts at: 1537 1812 3577 1537 Size: 275 1765 4090 Fragments arranged by size: 4090 1765 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2092 2831 3227 4060 1353 Size: 739 739 396 833 3423 Fragments arranged by size: 3423 833 739 739 396 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4717 5091 5237 387 Size: 1064 138 3128 374 146 1280 Fragments arranged by size: 3128 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2033 2163 3014 3168 4193 4790 5577 Size: 393 401 130 851 154 1025 597 787 Cuts at: 5577 5845 1239 Size: 268 1524 Fragments arranged by size: 1524 1025 851 787 597 401 393 268 154 130 EcoO109I rG'GnC_Cy Cuts at: 2136 2649 2136 Size: 513 5617 EcoRI G'AATT_C Cuts at: 3439 3817 3439 Size: 378 5752 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2908 3349 2908 Size: 441 5689 FspI TGC'GCA Cuts at: 4636 4636 Size: 6130 HaeII r_GCGC'y Cuts at: 1678 2815 5277 1678 Size: 1137 2462 2531 Fragments arranged by size: 2531 2462 1137 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2091 2123 2330 2362 2733 Size: 32 135 32 519 32 207 32 371 Cuts at: 2733 2765 2989 3021 3338 3370 4771 4803 4845 Size: 32 224 32 317 32 1401 32 42 Cuts at: 4845 4877 1373 Size: 32 2626 Fragments arranged by size: 2626 1401 519 371 317 224 207 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2533 2695 3474 3720 669 Size: 988 281 595 162 779 246 3079 Fragments arranged by size: 3079 988 779 595 281 246 162 HindIII A'AGCT_T Cuts at: 748 748 Size: 6130 HpaI GTT'AAC Cuts at: 3720 3720 Size: 6130 KpnI G_GTAC'C Cuts at: 3455 3455 Size: 6130 MfeI C'AATT_G Cuts at: 3729 3729 Size: 6130 MluI A'CGCG_T Cuts at: 6006 6006 Size: 6130 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5244 5428 1305 Size: 3939 184 2007 Fragments arranged by size: 3939 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4308 Fragments arranged by size: 4308 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2333 4088 4447 4606 518 Size: 648 1167 1755 359 159 2042 Fragments arranged by size: 2042 1755 1167 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2070 2153 2378 2558 2931 3546 4223 5371 Size: 464 83 225 180 373 615 677 1148 Cuts at: 5371 5616 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 615 464 373 245 225 180 83 NcoI C'CATG_G Cuts at: 513 1065 2004 2242 513 Size: 552 939 238 4401 Fragments arranged by size: 4401 939 552 238 NdeI CA'TA_TG Cuts at: 387 387 Size: 6130 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6130 NotI GC'GGCC_GC Cuts at: 3577 3577 Size: 6130 NspI r_CATG'y Cuts at: 1132 1229 2693 3488 5033 1132 Size: 97 1464 795 1545 2229 Fragments arranged by size: 2229 1545 1464 795 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6130 PciI A'CATG_T Cuts at: 5029 5029 Size: 6130 PflMI CCAn_nnn'nTGG Cuts at: 1108 2470 1108 Size: 1362 4768 PfoI T'CCnGG_A Cuts at: 2176 2176 Size: 6130 PmeI GTTT'AAAC Cuts at: 3434 3434 Size: 6130 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4199 4231 5712 5744 4199 Size: 32 1481 32 4585 Fragments arranged by size: 4585 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2136 2649 2136 Size: 513 5617 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6130 PsiI TTA'TAA Cuts at: 3700 3700 Size: 6130 PstI C_TGCA'G Cuts at: 830 3482 830 Size: 2652 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6130 PvuII CAG'CTG Cuts at: 2153 2558 2153 Size: 405 5725 SacII CC_GC'GG Cuts at: 2071 2379 2071 Size: 308 5822 SalI G'TCGA_C Cuts at: 1936 3472 1936 Size: 1536 4594 SanDI GG'GwC_CC Cuts at: 2649 2649 Size: 6130 SbfI CC_TGCA'GG Cuts at: 3482 3482 Size: 6130 ScaI AGT'ACT Cuts at: 4378 6090 4378 Size: 1712 4418 SfcI C'TryA_G Cuts at: 826 1045 2869 3478 4613 5294 5485 826 Size: 219 1824 609 1135 681 191 1471 Fragments arranged by size: 1824 1471 1135 681 609 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6130 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6130 SmaI CCC'GGG Cuts at: 1872 3457 1872 Size: 1585 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2911 4234 5135 5397 820 Size: 197 926 968 1323 901 262 1553 Fragments arranged by size: 1553 1323 968 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3921 493 Size: 3428 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6130 SphI G_CATG'C Cuts at: 1132 3488 1132 Size: 2356 3774 SspI AAT'ATT Cuts at: 5 52 4054 5 Size: 47 4002 2081 Fragments arranged by size: 4002 2081 47 StuI AGG'CCT Cuts at: 1517 2867 3086 1517 Size: 1350 219 4561 Fragments arranged by size: 4561 1350 219 StyI C'CwwG_G Cuts at: 513 1065 2004 2242 2482 2883 3569 513 Size: 552 939 238 240 401 686 3074 Fragments arranged by size: 3074 939 686 552 401 240 238 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2266 4157 4174 4327 4512 4957 325 Size: 1255 686 1891 17 153 185 445 1498 Fragments arranged by size: 1891 1498 1255 686 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 4376 6088 Size: 274 80 33 51 1443 324 2075 1712 Cuts at: 6088 96 Size: 138 Fragments arranged by size: 2075 1712 1443 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2391 2423 2798 2830 3049 3081 6090 6122 2391 Size: 32 375 32 219 32 3009 32 2399 Fragments arranged by size: 3009 2399 375 219 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2459 1316 Size: 1143 4987 XbaI T'CTAG_A Cuts at: 3466 3466 Size: 6130 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2329 2681 2329 Size: 352 5778 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6130 XmaI C'CCGG_G Cuts at: 1870 3455 1870 Size: 1585 4545 XmnI GAAnn'nnTTC Cuts at: 4259 4259 Size: 6130 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2626 3938 276 Size: 53 83 186 936 1092 1312 2468 Fragments arranged by size: 2468 1312 1092 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI