(Circular) (Six-base) MAPSORT of: pFN21AB3043.seq Check: 4362 from: 1 to: 8161 pFN21AB3043 8161 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5971 278 Size: 53 83 186 936 4435 2468 Fragments arranged by size: 4435 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3801 4659 4779 5504 1937 Size: 1864 858 120 725 4594 Fragments arranged by size: 4594 1864 858 725 120 Acc65I G'GTAC_C Cuts at: 5482 5482 Size: 8161 AclI AA'CG_TT Cuts at: 1563 6288 6661 1563 Size: 4725 373 3063 Fragments arranged by size: 4725 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2111 2604 2987 3052 4339 6224 7608 7876 Size: 479 493 383 65 1287 1885 1384 268 Cuts at: 7876 1632 Size: 1917 Fragments arranged by size: 1917 1885 1384 1287 493 479 383 268 65 AfeI AGC'GCT Cuts at: 3073 4517 5012 3073 Size: 1444 495 6222 Fragments arranged by size: 6222 1444 495 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7964 AflIII A'CryG_T Cuts at: 1203 2262 2352 2937 7060 8037 1203 Size: 1059 90 585 4123 977 1327 Fragments arranged by size: 4123 1327 1059 977 585 90 AgeI A'CCGG_T Cuts at: 6992 6992 Size: 8161 AhdI GACnn_n'nnGTC Cuts at: 2043 6890 2043 Size: 4847 3314 AleI CACnn'nnGTG Cuts at: 1166 2357 4565 1166 Size: 1191 2208 4762 Fragments arranged by size: 4762 2208 1191 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3537 1184 Size: 2353 5808 AlwNI CAG_nnn'CTG Cuts at: 2311 3686 3763 3963 4418 5904 7476 2311 Size: 1375 77 200 455 1486 1572 2996 Fragments arranged by size: 2996 1572 1486 1375 455 200 77 ApaI G_GGCC'C Cuts at: 3442 3631 4395 3442 Size: 189 764 7208 Fragments arranged by size: 7208 764 189 ApaLI G'TGCA_C Cuts at: 2330 6218 7374 2330 Size: 3888 1156 3117 Fragments arranged by size: 3888 3117 1156 ApoI r'AATT_y Cuts at: 1493 2588 4745 5129 5470 5696 5848 7957 1493 Size: 1095 2157 384 341 226 152 2109 1697 Fragments arranged by size: 2157 2109 1697 1095 384 341 226 152 AseI AT'TA_AT Cuts at: 160 1032 6715 160 Size: 872 5683 1606 Fragments arranged by size: 5683 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8161 AvaI C'yCGr_G Cuts at: 1870 1943 3462 4395 4987 5443 5486 1870 Size: 73 1519 933 592 456 43 4545 Fragments arranged by size: 4545 1519 933 592 456 73 43 BamHI G'GATC_C Cuts at: 2132 2384 5491 5858 2132 Size: 252 3107 367 4435 Fragments arranged by size: 4435 3107 367 252 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3271 3353 3365 4533 4921 Size: 325 217 627 1484 82 12 1168 388 Cuts at: 4921 5482 6937 618 Size: 561 1455 1842 Fragments arranged by size: 1842 1484 1455 1168 627 561 388 325 217 82 12 BanII G_rGCy'C Cuts at: 1391 2155 2229 2370 2740 3442 3508 3631 3820 Size: 764 74 141 370 702 66 123 189 Cuts at: 3820 4187 4395 5480 1391 Size: 367 208 1085 4072 Fragments arranged by size: 4072 1085 764 702 370 367 208 189 141 123 74 66 BbeI G_GCGC'C Cuts at: 3369 4537 3369 Size: 1168 6993 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2975 4066 928 Size: 971 1076 1091 5023 Fragments arranged by size: 5023 1091 1076 971 BbvCI CC'TCA_GC Cuts at: 2304 4343 2304 Size: 2039 6122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3343 3377 5326 5360 6352 6386 1903 Size: 34 1406 34 1949 34 992 34 3678 Fragments arranged by size: 3678 1949 1406 992 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6053 7263 6053 Size: 1210 6951 BclI T'GATC_A Cuts at: 1548 2932 3922 1548 Size: 1384 990 5787 Fragments arranged by size: 5787 1384 990 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3626 4930 6772 Size: 107 122 71 631 605 1954 1304 1842 Cuts at: 6772 136 Size: 1525 Fragments arranged by size: 1954 1842 1525 1304 631 605 122 107 71 BglII A'GATC_T Cuts at: 2997 5217 8156 2997 Size: 2220 2939 3002 Fragments arranged by size: 3002 2939 2220 BlpI GC'TnA_GC Cuts at: 3101 5040 5578 3101 Size: 1939 538 5684 Fragments arranged by size: 5684 1939 538 Bme1580I G_kGCm'C Cuts at: 1790 2334 3274 3356 3442 3488 3631 3828 4395 Size: 544 940 82 86 46 143 197 567 Cuts at: 4395 4926 6222 7378 1790 Size: 531 1296 1156 2573 Fragments arranged by size: 2573 1296 1156 940 567 544 531 197 143 86 82 46 BmgBI CAC'GTC Cuts at: 1318 3400 3616 1318 Size: 2082 216 5863 Fragments arranged by size: 5863 2082 216 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3133 4172 5892 Size: 416 53 83 399 24 1716 1039 1720 Cuts at: 5892 6850 442 Size: 958 1753 Fragments arranged by size: 1753 1720 1716 1039 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8161 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2539 3983 4570 5002 2539 Size: 1444 587 432 5698 Fragments arranged by size: 5698 1444 587 432 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3728 4490 4775 6821 1239 Size: 2489 762 285 2046 2579 Fragments arranged by size: 2579 2489 2046 762 285 Bpu10I CC'TnA_GC Cuts at: 2304 3839 4343 2304 Size: 1535 504 6122 Fragments arranged by size: 6122 1535 504 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2424 4679 6286 7151 7449 2424 Size: 2255 1607 865 298 3136 Fragments arranged by size: 3136 2255 1607 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4133 4810 5279 6824 882 Size: 617 2634 677 469 1545 2219 Fragments arranged by size: 2634 2219 1545 677 617 469 BsaAI yAC'GTr Cuts at: 493 1204 2355 2938 3829 4995 5952 493 Size: 711 1151 583 891 1166 957 2702 Fragments arranged by size: 2702 1166 1151 957 891 711 583 BsaBI GATnn'nnATC Cuts at: 8155 8155 Size: 8161 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3366 4534 5968 6350 Size: 53 83 186 936 1833 1168 1434 382 Cuts at: 6350 6985 275 Size: 635 1451 Fragments arranged by size: 1833 1451 1434 1168 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2592 4488 6594 6992 7266 7413 7824 2592 Size: 1896 2106 398 274 147 411 2929 Fragments arranged by size: 2929 2106 1896 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5343 5373 1178 Size: 30 165 30 3940 30 3966 Fragments arranged by size: 3966 3940 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3653 4546 4682 4906 1186 Size: 2467 893 136 224 4441 Fragments arranged by size: 4441 2467 893 224 136 BseYI C'CCAG_C Cuts at: 3583 3846 7364 7860 3583 Size: 263 3518 496 3884 Fragments arranged by size: 3884 3518 496 263 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 7588 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3009 4054 5611 6372 7400 1540 Size: 275 186 1008 1045 1557 761 1028 2301 Fragments arranged by size: 2301 1557 1045 1028 1008 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2334 3107 3986 4589 5210 6222 6307 7378 2334 Size: 773 879 603 621 1012 85 1071 3117 Fragments arranged by size: 3117 1071 1012 879 773 621 603 85 BsmI GAATG_Cn' Cuts at: 2752 5672 5765 2752 Size: 2920 93 5148 Fragments arranged by size: 5148 2920 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2155 2229 2334 2370 2740 3107 3274 Size: 399 365 74 105 36 370 367 167 Cuts at: 3274 3356 3442 3488 3508 3631 3820 3828 3986 Size: 82 86 46 20 123 189 8 158 Cuts at: 3986 4187 4395 4589 4926 5210 5480 6222 6307 Size: 201 208 194 337 284 270 742 85 Cuts at: 6307 7378 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 370 367 365 337 284 270 208 201 194 189 167 158 123 105 86 85 82 74 46 36 20 8 BspEI T'CCGG_A Cuts at: 2592 7824 2592 Size: 5232 2929 BspHI T'CATG_A Cuts at: 4362 6050 7780 4362 Size: 1688 1730 4743 Fragments arranged by size: 4743 1730 1688 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2230 5516 844 Size: 1386 3286 3489 Fragments arranged by size: 3489 3286 1386 BsrBI CCG'CTC Cuts at: 1394 1823 5326 6048 1394 Size: 429 3503 722 3507 Fragments arranged by size: 3507 3503 722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2082 2454 4324 6656 6830 65 Size: 1183 834 372 1870 2332 174 1396 Fragments arranged by size: 2332 1870 1396 1183 834 372 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 6805 6992 1700 Size: 110 2952 2043 187 2869 Fragments arranged by size: 2952 2869 2043 187 110 BsrGI T'GTAC_A Cuts at: 96 5385 96 Size: 5289 2872 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8161 BssSI C'ACGA_G Cuts at: 2928 4379 6221 7233 2928 Size: 1451 1842 1012 3856 Fragments arranged by size: 3856 1842 1451 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3832 4593 794 Size: 3038 761 4362 Fragments arranged by size: 4362 3038 761 BstBI TT'CG_AA Cuts at: 6965 6965 Size: 8161 BstEII G'GTnAC_C Cuts at: 1184 4456 4633 4789 4966 1184 Size: 3272 177 156 177 4379 Fragments arranged by size: 4379 3272 177 177 156 BstXI CCAn_nnnn'nTGG Cuts at: 2143 3752 3895 4909 2143 Size: 1609 143 1014 5395 Fragments arranged by size: 5395 1609 1014 143 BstYI r'GATC_y Cuts at: 1972 2132 2384 2757 2997 3683 4011 5217 5491 Size: 160 252 373 240 686 328 1206 274 Cuts at: 5491 5858 6243 6260 7712 7798 7810 8156 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1206 686 385 373 367 346 328 274 252 240 160 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2823 3679 2823 Size: 856 7305 BtgI C'CryG_G Cuts at: 513 1065 2004 2247 2688 3393 4798 5058 513 Size: 552 939 243 441 705 1405 260 3616 Fragments arranged by size: 3616 1405 939 705 552 441 260 243 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2896 3317 5059 487 Size: 683 56 1670 421 1742 3589 Fragments arranged by size: 3589 1742 1670 683 421 56 BtsI GCAGTG_nn' Cuts at: 2350 2887 3521 5677 6471 6491 2350 Size: 537 634 2156 794 20 4020 Fragments arranged by size: 4020 2156 794 634 537 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8161 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8126 DraI TTT'AAA Cuts at: 5465 5465 Size: 8161 DraIII CAC_nnn'GTG Cuts at: 3767 3767 Size: 8161 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7168 809 Size: 507 5852 1802 Fragments arranged by size: 5852 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2685 3618 4051 Size: 54 1425 50 275 18 855 933 433 Cuts at: 4051 4250 5608 6497 8126 8 Size: 199 1358 889 1629 43 Fragments arranged by size: 1629 1425 1358 933 889 855 433 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4051 5608 1537 Size: 275 2239 1557 4090 Fragments arranged by size: 4090 2239 1557 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3974 4286 4577 6091 1353 Size: 2621 312 291 1514 3423 Fragments arranged by size: 3423 2621 1514 312 291 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2222 2521 2689 4277 6748 7122 Size: 1064 138 633 299 168 1588 2471 374 Cuts at: 7122 7268 387 Size: 146 1280 Fragments arranged by size: 2471 1588 1280 1064 633 374 299 168 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2111 2604 2987 3052 3728 4339 4490 Size: 393 479 493 383 65 676 611 151 Cuts at: 4490 4775 6224 6821 7608 7876 1239 Size: 285 1449 597 787 268 1524 Fragments arranged by size: 1524 1449 787 676 611 597 493 479 393 383 285 268 151 65 EcoNI CCTnn'n_nnAGG Cuts at: 3493 3493 Size: 8161 EcoO109I rG'GnC_Cy Cuts at: 2242 2422 3378 3438 3439 3627 3628 3778 3843 Size: 180 956 60 1 188 1 150 65 Cuts at: 3843 4174 4392 4575 5053 5274 5308 5438 2242 Size: 331 218 183 478 221 34 130 4965 Fragments arranged by size: 4965 956 478 331 221 218 188 183 180 150 130 65 60 34 1 1 EcoRI G'AATT_C Cuts at: 2588 4745 5129 5470 5848 2588 Size: 2157 384 341 378 4901 Fragments arranged by size: 4901 2157 384 378 341 EcoRV GAT'ATC Cuts at: 3281 3281 Size: 8161 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2767 5029 5413 2767 Size: 2262 384 5515 Fragments arranged by size: 5515 2262 384 FspI TGC'GCA Cuts at: 6667 6667 Size: 8161 HaeII r_GCGC'y Cuts at: 1678 3075 3369 4519 4537 5014 7308 1678 Size: 1397 294 1150 18 477 2294 2531 Fragments arranged by size: 2531 2294 1397 1150 477 294 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2029 2061 2826 2858 3675 Size: 32 135 32 457 32 765 32 817 Cuts at: 3675 3707 4646 4678 5343 5375 6802 6834 6876 Size: 32 939 32 665 32 1427 32 42 Cuts at: 6876 6908 1373 Size: 32 2626 Fragments arranged by size: 2626 1427 939 817 765 665 457 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2028 5505 5751 669 Size: 988 281 90 3477 246 3079 Fragments arranged by size: 3477 3079 988 281 246 90 HindIII A'AGCT_T Cuts at: 748 748 Size: 8161 HpaI GTT'AAC Cuts at: 2028 5751 2028 Size: 3723 4438 KasI G'GCGC_C Cuts at: 3365 4533 3365 Size: 1168 6993 KpnI G_GTAC'C Cuts at: 5486 5486 Size: 8161 MfeI C'AATT_G Cuts at: 5760 5760 Size: 8161 MluI A'CGCG_T Cuts at: 8037 8037 Size: 8161 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2128 2412 3360 4774 5052 7275 7459 1305 Size: 823 284 948 1414 278 2223 184 2007 Fragments arranged by size: 2223 2007 1414 948 823 284 278 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3620 10 Size: 54 1425 343 1788 4551 Fragments arranged by size: 4551 1788 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2861 3176 3323 4565 6119 6478 Size: 648 1191 504 315 147 1242 1554 359 Cuts at: 6478 6637 518 Size: 159 2042 Fragments arranged by size: 2042 1554 1242 1191 648 504 359 315 159 147 MspA1I CmG'CkG Cuts at: 1606 2308 2665 3712 4593 5213 5577 6254 7402 Size: 702 357 1047 881 620 364 677 1148 Cuts at: 7402 7647 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1047 881 702 677 620 364 357 245 NarI GG'CG_CC Cuts at: 3366 4534 3366 Size: 1168 6993 NcoI C'CATG_G Cuts at: 513 1065 2004 4798 513 Size: 552 939 2794 3876 Fragments arranged by size: 3876 2794 939 552 NdeI CA'TA_TG Cuts at: 387 4949 387 Size: 4562 3599 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8161 NotI GC'GGCC_GC Cuts at: 5608 5608 Size: 8161 NspI r_CATG'y Cuts at: 1132 1229 2266 3328 4726 5519 7064 1132 Size: 97 1037 1062 1398 793 1545 2229 Fragments arranged by size: 2229 1545 1398 1062 1037 793 97 PasI CC'CwG_GG Cuts at: 1348 2269 3495 3552 3820 3888 1348 Size: 921 1226 57 268 68 5621 Fragments arranged by size: 5621 1226 921 268 68 57 PciI A'CATG_T Cuts at: 2262 7060 2262 Size: 4798 3363 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8161 PfoI T'CCnGG_A Cuts at: 4617 5121 4617 Size: 504 7657 PmeI GTTT'AAAC Cuts at: 5465 5465 Size: 8161 PmlI CAC'GTG Cuts at: 2938 3829 2938 Size: 891 7270 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4502 4534 6230 6262 7743 7775 4502 Size: 32 1696 32 1481 32 4888 Fragments arranged by size: 4888 1696 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2242 3778 3843 4174 5274 5308 5438 2242 Size: 1536 65 331 1100 34 130 4965 Fragments arranged by size: 4965 1536 1100 331 130 65 34 PshAI GACnn'nnGTC Cuts at: 1613 4112 4454 1613 Size: 2499 342 5320 Fragments arranged by size: 5320 2499 342 PsiI TTA'TAA Cuts at: 5731 5731 Size: 8161 PspOMI G'GGCC_C Cuts at: 3438 3627 4391 3438 Size: 189 764 7208 Fragments arranged by size: 7208 764 189 PstI C_TGCA'G Cuts at: 830 2241 4598 5513 830 Size: 1411 2357 915 3478 Fragments arranged by size: 3478 2357 1411 915 PvuI CG_AT'CG Cuts at: 2001 3009 2001 Size: 1008 7153 PvuII CAG'CTG Cuts at: 2308 2665 4593 5213 2308 Size: 357 1928 620 5256 Fragments arranged by size: 5256 1928 620 357 SalI G'TCGA_C Cuts at: 1936 5503 1936 Size: 3567 4594 SanDI GG'GwC_CC Cuts at: 5438 5438 Size: 8161 SapI GCTCTTCn'nnn_ Cuts at: 3974 4577 3974 Size: 603 7558 SbfI CC_TGCA'GG Cuts at: 2241 5513 2241 Size: 3272 4889 ScaI AGT'ACT Cuts at: 2579 3855 6409 8121 2579 Size: 1276 2554 1712 2619 Fragments arranged by size: 2619 2554 1712 1276 SexAI A'CCwGG_T Cuts at: 3423 3423 Size: 8161 SfcI C'TryA_G Cuts at: 826 1045 2206 2237 3810 4151 4444 4594 5509 Size: 219 1161 31 1573 341 293 150 915 Cuts at: 5509 6644 7325 7516 826 Size: 1135 681 191 1471 Fragments arranged by size: 1573 1471 1161 1135 915 681 341 293 219 191 150 31 SfiI GGCCn_nnn'nGGCC Cuts at: 3626 3626 Size: 8161 SfoI GGC'GCC Cuts at: 3367 4535 3367 Size: 1168 6993 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8161 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8161 SmaI CCC'GGG Cuts at: 1872 4397 5445 5488 1872 Size: 2525 1048 43 4545 Fragments arranged by size: 4545 2525 1048 43 SmlI C'TyrA_G Cuts at: 820 1017 1943 2439 4694 4987 6265 7166 7428 Size: 197 926 496 2255 293 1278 901 262 Cuts at: 7428 820 Size: 1553 Fragments arranged by size: 2255 1553 1278 926 901 496 293 262 197 SnaBI TAC'GTA Cuts at: 493 5952 493 Size: 5459 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8161 SphI G_CATG'C Cuts at: 1132 5519 1132 Size: 4387 3774 SspI AAT'ATT Cuts at: 5 52 6085 5 Size: 47 6033 2081 Fragments arranged by size: 6033 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8161 StyI C'CwwG_G Cuts at: 513 1065 2004 2799 4675 4798 5004 5600 513 Size: 552 939 795 1876 123 206 596 3074 Fragments arranged by size: 3074 1876 939 795 596 552 206 123 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3371 4376 6188 6205 6358 6543 6988 Size: 1255 1791 1005 1812 17 153 185 445 Cuts at: 6988 325 Size: 1498 Fragments arranged by size: 1812 1791 1498 1255 1005 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2577 3853 5385 Size: 274 80 33 51 1443 600 1276 1532 Cuts at: 5385 6407 8119 96 Size: 1022 1712 138 Fragments arranged by size: 1712 1532 1443 1276 1022 600 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2124 2156 8121 8153 2124 Size: 32 5965 32 2132 Fragments arranged by size: 5965 2132 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3425 1316 Size: 2109 6052 XbaI T'CTAG_A Cuts at: 5497 5497 Size: 8161 XhoI C'TCGA_G Cuts at: 1943 4987 1943 Size: 3044 5117 XmaI C'CCGG_G Cuts at: 1870 4395 5443 5486 1870 Size: 2525 1048 43 4545 Fragments arranged by size: 4545 2525 1048 43 XmnI GAAnn'nnTTC Cuts at: 6290 6290 Size: 8161 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5969 276 Size: 53 83 186 936 4435 2468 Fragments arranged by size: 4435 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoICRI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SrfI SwaI XcmI