(Circular) (Six-base) MAPSORT of: pFN21AB3030.seq Check: 7960 from: 1 to: 7456 pFN21AB3030 7456 bp With 182 enzymes: SgfI * October 20, 2009 10:38 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5266 278 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3240 3252 3283 4799 1937 Size: 1303 12 31 1516 4594 Fragments arranged by size: 4594 1516 1303 31 12 Acc65I G'GTAC_C Cuts at: 4777 4777 Size: 7456 AclI AA'CG_TT Cuts at: 1563 5583 5956 1563 Size: 4020 373 3063 Fragments arranged by size: 4020 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3935 5519 6903 7171 1632 Size: 2303 1584 1384 268 1917 Fragments arranged by size: 2303 1917 1584 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7259 AflIII A'CryG_T Cuts at: 1203 3549 6355 7332 1203 Size: 2346 2806 977 1327 Fragments arranged by size: 2806 2346 1327 977 AgeI A'CCGG_T Cuts at: 2996 6287 2996 Size: 3291 4165 AhdI GACnn_n'nnGTC Cuts at: 6185 6185 Size: 7456 AleI CACnn'nnGTG Cuts at: 1166 2204 2673 3548 4478 4721 1166 Size: 1038 469 875 930 243 3901 Fragments arranged by size: 3901 1038 930 875 469 243 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2040 3901 1184 Size: 856 1861 4739 Fragments arranged by size: 4739 1861 856 AlwNI CAG_nnn'CTG Cuts at: 3457 5199 6771 3457 Size: 1742 1572 4142 Fragments arranged by size: 4142 1742 1572 ApaLI G'TGCA_C Cuts at: 4471 5513 6669 4471 Size: 1042 1156 5258 Fragments arranged by size: 5258 1156 1042 ApoI r'AATT_y Cuts at: 1493 3613 4175 4765 4991 5143 7252 1493 Size: 2120 562 590 226 152 2109 1697 Fragments arranged by size: 2120 2109 1697 590 562 226 152 AseI AT'TA_AT Cuts at: 160 1032 6010 160 Size: 872 4978 1606 Fragments arranged by size: 4978 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 AvaI C'yCGr_G Cuts at: 1870 1943 2866 3761 4210 4781 1870 Size: 73 923 895 449 571 4545 Fragments arranged by size: 4545 923 895 571 449 73 BamHI G'GATC_C Cuts at: 4786 5153 4786 Size: 367 7089 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2372 3307 3710 4777 6232 Size: 325 217 627 585 935 403 1067 1455 Cuts at: 6232 618 Size: 1842 Fragments arranged by size: 1842 1455 1067 935 627 585 403 325 217 BanII G_rGCy'C Cuts at: 1391 2046 2310 2858 3726 4775 1391 Size: 655 264 548 868 1049 4072 Fragments arranged by size: 4072 1049 868 655 548 264 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3425 4014 928 Size: 971 1526 589 4370 Fragments arranged by size: 4370 1526 971 589 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5647 5681 1903 Size: 34 3710 34 3678 Fragments arranged by size: 3710 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4530 5348 6558 4530 Size: 818 1210 5428 Fragments arranged by size: 5428 1210 818 BclI T'GATC_A Cuts at: 1548 2892 4236 1548 Size: 1344 1344 4768 Fragments arranged by size: 4768 1344 1344 BfrBI ATG'CAT Cuts at: 4630 4630 Size: 7456 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3173 4490 6067 Size: 107 122 71 631 605 1501 1317 1577 Cuts at: 6067 136 Size: 1525 Fragments arranged by size: 1577 1525 1501 1317 631 605 122 107 71 BglII A'GATC_T Cuts at: 2118 2334 3678 7451 2118 Size: 216 1344 3773 2123 Fragments arranged by size: 3773 2123 1344 216 BlpI GC'TnA_GC Cuts at: 2975 3440 4873 2975 Size: 465 1433 5558 Fragments arranged by size: 5558 1433 465 Bme1580I G_kGCm'C Cuts at: 1790 2699 3312 3412 3526 4475 5517 6673 1790 Size: 909 613 100 114 949 1042 1156 2573 Fragments arranged by size: 2573 1156 1042 949 909 613 114 100 BmgBI CAC'GTC Cuts at: 1318 3901 1318 Size: 2583 4873 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2462 5187 6145 Size: 416 53 83 399 24 1045 2725 958 Cuts at: 6145 442 Size: 1753 Fragments arranged by size: 2725 1753 1045 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7456 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2232 2232 Size: 7456 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2202 2294 3479 6116 1239 Size: 963 92 1185 2637 2579 Fragments arranged by size: 2637 2579 1185 963 92 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3839 4316 4451 5581 6446 6744 3839 Size: 477 135 1130 865 298 4551 Fragments arranged by size: 4551 1130 865 477 298 135 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2099 2515 2690 3002 6119 882 Size: 617 600 416 175 312 3117 2219 Fragments arranged by size: 3117 2219 617 600 416 312 175 BsaAI yAC'GTr Cuts at: 493 1204 3550 5247 493 Size: 711 2346 1697 2702 Fragments arranged by size: 2702 2346 1697 711 BsaBI GATnn'nnATC Cuts at: 2771 7450 2771 Size: 4679 2777 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5263 5645 6280 275 Size: 53 83 186 936 3730 382 635 1451 Fragments arranged by size: 3730 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2571 2996 3051 3120 5889 6287 6561 6708 7119 Size: 425 55 69 2769 398 274 147 411 Cuts at: 7119 2571 Size: 2908 Fragments arranged by size: 2908 2769 425 411 398 274 147 69 55 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7231 Fragments arranged by size: 7231 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2602 2966 3227 3484 3842 3953 4082 1186 Size: 1416 364 261 257 358 111 129 4560 Fragments arranged by size: 4560 1416 364 358 261 257 129 111 BseYI C'CCAG_C Cuts at: 2376 4497 6659 7155 2376 Size: 2121 2162 496 2677 Fragments arranged by size: 2677 2162 2121 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 3381 1163 Size: 573 345 1300 5238 Fragments arranged by size: 5238 1300 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 4906 5667 6695 1540 Size: 275 186 1000 1905 761 1028 2301 Fragments arranged by size: 2301 1905 1028 1000 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2046 3726 4439 4475 5517 5602 6673 2046 Size: 1680 713 36 1042 85 1071 2829 Fragments arranged by size: 2829 1680 1071 1042 713 85 36 BsmI GAATG_Cn' Cuts at: 2405 4967 5060 2405 Size: 2562 93 4801 Fragments arranged by size: 4801 2562 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2547 2547 Size: 7456 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2046 2310 2699 2858 3312 3412 3526 Size: 399 256 264 389 159 454 100 114 Cuts at: 3526 3726 4439 4475 4775 5517 5602 6673 1391 Size: 200 713 36 300 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 713 454 399 389 300 264 256 200 159 114 100 85 36 BspEI T'CCGG_A Cuts at: 2571 3051 7119 2571 Size: 480 4068 2908 Fragments arranged by size: 4068 2908 480 BspHI T'CATG_A Cuts at: 5345 7075 5345 Size: 1730 5726 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4811 844 Size: 3967 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3217 3336 4327 5343 1394 Size: 429 1394 119 991 1016 3507 Fragments arranged by size: 3507 1394 1016 991 429 119 BsrDI GCAATG_nn' Cuts at: 65 1248 2391 4057 4084 5951 6125 65 Size: 1183 1143 1666 27 1867 174 1396 Fragments arranged by size: 1867 1666 1396 1183 1143 174 27 BsrFI r'CCGG_y Cuts at: 1700 1810 2996 3387 3930 6100 6287 1700 Size: 110 1186 391 543 2170 187 2869 Fragments arranged by size: 2869 2170 1186 543 391 187 110 BsrGI T'GTAC_A Cuts at: 96 2904 96 Size: 2808 4648 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7456 BssSI C'ACGA_G Cuts at: 5516 6528 5516 Size: 1012 6444 BstAPI GCAn_nnn'nTGC Cuts at: 794 2712 4056 794 Size: 1918 1344 4194 Fragments arranged by size: 4194 1918 1344 BstBI TT'CG_AA Cuts at: 6260 6260 Size: 7456 BstEII G'GTnAC_C Cuts at: 1184 3904 1184 Size: 2720 4736 BstXI CCAn_nnnn'nTGG Cuts at: 2542 4505 2542 Size: 1963 5493 BstYI r'GATC_y Cuts at: 1972 2118 2254 2334 3123 3678 4786 5153 5538 Size: 146 136 80 789 555 1108 367 385 Cuts at: 5538 5555 7007 7093 7105 7451 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1108 789 555 385 367 346 146 136 86 80 17 12 Bsu36I CC'TnA_GG Cuts at: 2349 2349 Size: 7456 BtgI C'CryG_G Cuts at: 513 1065 3366 4549 513 Size: 552 2301 1183 3420 Fragments arranged by size: 3420 2301 1183 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2769 3333 3733 487 Size: 683 56 1543 564 400 4210 Fragments arranged by size: 4210 1543 683 564 400 56 BtsI GCAGTG_nn' Cuts at: 2190 2374 3355 3384 4716 4972 5766 5786 2190 Size: 184 981 29 1332 256 794 20 3860 Fragments arranged by size: 3860 1332 981 794 256 184 29 20 ClaI AT'CG_AT Cuts at: 1554 4281 1554 Size: 2727 4729 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2518 2553 529 Size: 35 1954 35 5432 Fragments arranged by size: 5432 1954 35 35 DraI TTT'AAA Cuts at: 4760 4760 Size: 7456 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3248 3737 6463 809 Size: 507 1932 489 2726 1802 Fragments arranged by size: 2726 1932 1802 507 489 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2943 3111 3174 Size: 54 1425 50 275 18 1113 168 63 Cuts at: 3174 3393 4518 4903 5792 7421 8 Size: 219 1125 385 889 1629 43 Fragments arranged by size: 1629 1425 1125 1113 889 385 275 219 168 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4903 1537 Size: 275 3091 4090 Fragments arranged by size: 4090 3091 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2718 4095 4310 5386 1353 Size: 1365 1377 215 1076 3423 Fragments arranged by size: 3423 1377 1365 1076 215 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2779 3139 4123 6043 6417 6563 Size: 1064 138 1190 360 984 1920 374 146 Cuts at: 6563 387 Size: 1280 Fragments arranged by size: 1920 1280 1190 1064 984 374 360 146 138 EcoICRI GAG'CTC Cuts at: 2044 3724 2044 Size: 1680 5776 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2202 2294 3479 3935 5519 6116 6903 Size: 393 570 92 1185 456 1584 597 787 Cuts at: 6903 7171 1239 Size: 268 1524 Fragments arranged by size: 1584 1524 1185 787 597 570 456 393 268 92 EcoNI CCTnn'n_nnAGG Cuts at: 2206 3914 2206 Size: 1708 5748 EcoO109I rG'GnC_Cy Cuts at: 2790 3067 3622 4134 4590 4645 2790 Size: 277 555 512 456 55 5601 Fragments arranged by size: 5601 555 512 456 277 55 EcoRI G'AATT_C Cuts at: 4765 5143 4765 Size: 378 7078 EcoRV GAT'ATC Cuts at: 2605 2605 Size: 7456 FspI TGC'GCA Cuts at: 3163 4076 5962 3163 Size: 913 1886 4657 Fragments arranged by size: 4657 1886 913 HaeII r_GCGC'y Cuts at: 1678 4712 6603 1678 Size: 3034 1891 2531 Fragments arranged by size: 3034 2531 1891 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2314 2346 2792 2824 2840 Size: 32 135 32 742 32 446 32 16 Cuts at: 2840 2872 4302 4334 6097 6129 6171 6203 1373 Size: 32 1430 32 1763 32 42 32 2626 Fragments arranged by size: 2626 1763 1430 742 446 135 42 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 2071 3241 4800 5046 669 Size: 988 281 133 1170 1559 246 3079 Fragments arranged by size: 3079 1559 1170 988 281 246 133 HindIII A'AGCT_T Cuts at: 748 748 Size: 7456 HpaI GTT'AAC Cuts at: 5046 5046 Size: 7456 KpnI G_GTAC'C Cuts at: 4781 4781 Size: 7456 MfeI C'AATT_G Cuts at: 5055 5055 Size: 7456 MluI A'CGCG_T Cuts at: 7332 7332 Size: 7456 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3961 6570 6754 1305 Size: 2656 2609 184 2007 Fragments arranged by size: 2656 2609 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2945 3113 10 Size: 54 1425 343 1113 168 4353 Fragments arranged by size: 4353 1425 1113 343 168 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2673 2741 3519 3548 3773 4478 Size: 648 1038 469 68 778 29 225 705 Cuts at: 4478 4721 5414 5773 5932 518 Size: 243 693 359 159 2042 Fragments arranged by size: 2042 1038 778 705 693 648 469 359 243 225 159 68 29 MspA1I CmG'CkG Cuts at: 1606 2380 3439 3454 4523 4695 4872 5549 6697 Size: 774 1059 15 1069 172 177 677 1148 Cuts at: 6697 6942 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 1069 1059 774 677 245 177 172 15 NcoI C'CATG_G Cuts at: 513 1065 3366 513 Size: 552 2301 4603 Fragments arranged by size: 4603 2301 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7456 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7456 NotI GC'GGCC_GC Cuts at: 4903 4903 Size: 7456 NsiI A_TGCA'T Cuts at: 4632 4632 Size: 7456 NspI r_CATG'y Cuts at: 1132 1229 2092 4814 6359 1132 Size: 97 863 2722 1545 2229 Fragments arranged by size: 2722 2229 1545 863 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7456 PciI A'CATG_T Cuts at: 6355 6355 Size: 7456 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 3457 1108 Size: 1209 1140 5107 Fragments arranged by size: 5107 1209 1140 PmeI GTTT'AAAC Cuts at: 4760 4760 Size: 7456 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5525 5557 7038 7070 5525 Size: 32 1481 32 5911 Fragments arranged by size: 5911 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3067 3622 4590 4645 3067 Size: 555 968 55 5878 Fragments arranged by size: 5878 968 555 55 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7456 PsiI TTA'TAA Cuts at: 5026 5026 Size: 7456 PspXI vC'TCGA_Gb Cuts at: 3761 3761 Size: 7456 PstI C_TGCA'G Cuts at: 830 2182 3189 3438 4680 4808 830 Size: 1352 1007 249 1242 128 3478 Fragments arranged by size: 3478 1352 1242 1007 249 128 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7456 PvuII CAG'CTG Cuts at: 2380 3439 3454 2380 Size: 1059 15 6382 Fragments arranged by size: 6382 1059 15 SacI G_AGCT'C Cuts at: 2046 3726 2046 Size: 1680 5776 SalI G'TCGA_C Cuts at: 1936 3239 4798 1936 Size: 1303 1559 4594 Fragments arranged by size: 4594 1559 1303 SanDI GG'GwC_CC Cuts at: 4590 4590 Size: 7456 SapI GCTCTTCn'nnn_ Cuts at: 4310 4310 Size: 7456 SbfI CC_TGCA'GG Cuts at: 4808 4808 Size: 7456 ScaI AGT'ACT Cuts at: 5704 7416 5704 Size: 1712 5744 SfcI C'TryA_G Cuts at: 826 1045 2178 3185 3434 4676 4804 5939 6620 Size: 219 1133 1007 249 1242 128 1135 681 Cuts at: 6620 6811 826 Size: 191 1471 Fragments arranged by size: 1471 1242 1135 1133 1007 681 249 219 191 128 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7456 SgrAI Cr'CCGG_yG Cuts at: 1700 3930 1700 Size: 2230 5226 SmaI CCC'GGG Cuts at: 1872 2868 4212 4783 1872 Size: 996 1344 571 4545 Fragments arranged by size: 4545 1344 996 571 SmlI C'TyrA_G Cuts at: 820 1017 1943 3761 3854 4331 4430 5560 6461 Size: 197 926 1818 93 477 99 1130 901 Cuts at: 6461 6723 820 Size: 262 1553 Fragments arranged by size: 1818 1553 1130 926 901 477 262 197 99 93 SnaBI TAC'GTA Cuts at: 493 5247 493 Size: 4754 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7456 SphI G_CATG'C Cuts at: 1132 4814 1132 Size: 3682 3774 SspI AAT'ATT Cuts at: 5 52 5380 5 Size: 47 5328 2081 Fragments arranged by size: 5328 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7456 StyI C'CwwG_G Cuts at: 513 1065 2520 2946 3366 3594 3601 4895 513 Size: 552 1455 426 420 228 7 1294 3074 Fragments arranged by size: 3074 1455 1294 552 426 420 228 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2275 2390 4244 4568 5483 5500 5653 Size: 1255 695 115 1854 324 915 17 153 Cuts at: 5653 5838 6283 325 Size: 185 445 1498 Fragments arranged by size: 1854 1498 1255 915 695 445 324 185 153 115 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2904 3765 5702 Size: 274 80 33 51 1443 927 861 1937 Cuts at: 5702 7414 96 Size: 1712 138 Fragments arranged by size: 1937 1712 1443 927 861 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3046 3078 3640 3672 7416 7448 3046 Size: 32 562 32 3744 32 3054 Fragments arranged by size: 3744 3054 562 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3977 1316 Size: 2661 4795 XbaI T'CTAG_A Cuts at: 4792 4792 Size: 7456 XhoI C'TCGA_G Cuts at: 1943 3761 1943 Size: 1818 5638 XmaI C'CCGG_G Cuts at: 1870 2866 4210 4781 1870 Size: 996 1344 571 4545 Fragments arranged by size: 4545 1344 996 571 XmnI GAAnn'nnTTC Cuts at: 5585 5585 Size: 7456 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5264 276 Size: 53 83 186 936 3730 2468 Fragments arranged by size: 3730 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI