(Circular) (Six-base) MAPSORT of: pFN21AB3013.seq Check: 7896 from: 1 to: 6073 pFN21AB3013 6073 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3883 278 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2770 3222 3416 1937 Size: 833 452 194 4594 Fragments arranged by size: 4594 833 452 194 Acc65I G'GTAC_C Cuts at: 3394 3394 Size: 6073 AclI AA'CG_TT Cuts at: 1563 4200 4573 1563 Size: 2637 373 3063 Fragments arranged by size: 3063 2637 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2825 4136 5520 5788 1632 Size: 1193 1311 1384 268 1917 Fragments arranged by size: 1917 1384 1311 1193 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5876 AflIII A'CryG_T Cuts at: 1203 4972 5949 1203 Size: 3769 977 1327 Fragments arranged by size: 3769 1327 977 AgeI A'CCGG_T Cuts at: 4904 4904 Size: 6073 AhdI GACnn_n'nnGTC Cuts at: 4802 4802 Size: 6073 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6073 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6073 AlwNI CAG_nnn'CTG Cuts at: 2803 3148 3298 3816 5388 2803 Size: 345 150 518 1572 3488 Fragments arranged by size: 3488 1572 518 345 150 ApaLI G'TGCA_C Cuts at: 4130 5286 4130 Size: 1156 4917 ApoI r'AATT_y Cuts at: 1493 2555 2639 2898 3382 3608 3760 5869 1493 Size: 1062 84 259 484 226 152 2109 1697 Fragments arranged by size: 2109 1697 1062 484 259 226 152 84 AseI AT'TA_AT Cuts at: 160 1032 2375 3308 4627 160 Size: 872 1343 933 1319 1606 Fragments arranged by size: 1606 1343 1319 933 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 AvaI C'yCGr_G Cuts at: 1870 1943 2090 3398 1870 Size: 73 147 1308 4545 Fragments arranged by size: 4545 1308 147 73 BamHI G'GATC_C Cuts at: 2717 3262 3403 3770 2717 Size: 545 141 367 5020 Fragments arranged by size: 5020 545 367 141 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3394 4849 618 Size: 325 217 627 1607 1455 1842 Fragments arranged by size: 1842 1607 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3392 1391 Size: 2001 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2125 928 Size: 971 226 4876 Fragments arranged by size: 4876 971 226 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4264 4298 1903 Size: 34 2327 34 3678 Fragments arranged by size: 3678 2327 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3965 5175 3965 Size: 1210 4863 BclI T'GATC_A Cuts at: 1548 1548 Size: 6073 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4684 136 Size: 107 122 71 631 605 3012 1525 Fragments arranged by size: 3012 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6068 6068 Size: 6073 BlpI GC'TnA_GC Cuts at: 3490 3490 Size: 6073 Bme1580I G_kGCm'C Cuts at: 1790 4134 5290 1790 Size: 2344 1156 2573 Fragments arranged by size: 2573 2344 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3804 4762 442 Size: 416 53 83 399 24 2387 958 1753 Fragments arranged by size: 2387 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2108 1056 Size: 1052 5021 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2509 2509 Size: 6073 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4733 1239 Size: 3494 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3023 4198 5063 5361 3023 Size: 1175 865 298 3735 Fragments arranged by size: 3735 1175 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3247 3294 4736 882 Size: 617 1748 47 1442 2219 Fragments arranged by size: 2219 1748 1442 617 47 BsaAI yAC'GTr Cuts at: 493 1204 3864 493 Size: 711 2660 2702 Fragments arranged by size: 2702 2660 711 BsaBI GATnn'nnATC Cuts at: 6067 6067 Size: 6073 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3880 4262 4897 275 Size: 53 83 186 936 2347 382 635 1451 Fragments arranged by size: 2347 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4506 4904 5178 5325 5736 4506 Size: 398 274 147 411 4843 Fragments arranged by size: 4843 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5848 Fragments arranged by size: 5848 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3365 1186 Size: 2179 3894 BseYI C'CCAG_C Cuts at: 5276 5772 5276 Size: 496 5577 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5500 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3523 4284 5312 1540 Size: 275 186 1522 761 1028 2301 Fragments arranged by size: 2301 1522 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4134 4219 5290 4134 Size: 85 1071 4917 Fragments arranged by size: 4917 1071 85 BsmI GAATG_Cn' Cuts at: 3584 3677 3584 Size: 93 5980 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3392 4134 4219 5290 1391 Size: 399 1602 742 85 1071 2174 Fragments arranged by size: 2174 1602 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5736 5736 Size: 6073 BspHI T'CATG_A Cuts at: 3962 5692 3962 Size: 1730 4343 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3428 844 Size: 2584 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2026 3960 1394 Size: 429 203 1934 3507 Fragments arranged by size: 3507 1934 429 203 BsrDI GCAATG_nn' Cuts at: 65 1248 4568 4742 65 Size: 1183 3320 174 1396 Fragments arranged by size: 3320 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4717 4904 1700 Size: 110 2907 187 2869 Fragments arranged by size: 2907 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6073 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6073 BssSI C'ACGA_G Cuts at: 4133 5145 4133 Size: 1012 5061 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6073 BstBI TT'CG_AA Cuts at: 2605 4877 2605 Size: 2272 3801 BstEII G'GTnAC_C Cuts at: 1184 2395 1184 Size: 1211 4862 BstYI r'GATC_y Cuts at: 1972 2717 2914 3151 3262 3403 3770 4155 4172 Size: 745 197 237 111 141 367 385 17 Cuts at: 4172 5624 5710 5722 6068 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 745 385 367 346 237 197 141 111 86 17 12 BstZ17I GTA'TAC Cuts at: 2771 2771 Size: 6073 BtgI C'CryG_G Cuts at: 513 1065 2004 2070 513 Size: 552 939 66 4516 Fragments arranged by size: 4516 939 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5334 Fragments arranged by size: 5334 683 56 BtsI GCAGTG_nn' Cuts at: 3589 4383 4403 3589 Size: 794 20 5259 Fragments arranged by size: 5259 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6073 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2947 2982 529 Size: 35 2383 35 3620 Fragments arranged by size: 3620 2383 35 35 DraI TTT'AAA Cuts at: 2922 3377 2922 Size: 455 5618 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5080 809 Size: 507 3764 1802 Fragments arranged by size: 3764 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3102 3520 4409 Size: 54 1425 50 275 18 1272 418 889 Cuts at: 4409 6038 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1272 889 418 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3520 1537 Size: 275 1708 4090 Fragments arranged by size: 4090 1708 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2801 4003 1353 Size: 1448 1202 3423 Fragments arranged by size: 3423 1448 1202 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2024 2299 4660 5034 5180 387 Size: 1064 138 435 275 2361 374 146 1280 Fragments arranged by size: 2361 1280 1064 435 374 275 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2825 4136 4733 5520 5788 1239 Size: 393 1193 1311 597 787 268 1524 Fragments arranged by size: 1524 1311 1193 787 597 393 268 EcoRI G'AATT_C Cuts at: 3382 3760 3382 Size: 378 5695 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2138 3017 3226 2138 Size: 879 209 4985 Fragments arranged by size: 4985 879 209 FspI TGC'GCA Cuts at: 4579 4579 Size: 6073 HaeII r_GCGC'y Cuts at: 1678 2070 2086 5220 1678 Size: 392 16 3134 2531 Fragments arranged by size: 3134 2531 392 16 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2003 2035 2171 2203 3349 Size: 32 135 32 431 32 136 32 1146 Cuts at: 3349 3381 4714 4746 4788 4820 1373 Size: 32 1333 32 42 32 2626 Fragments arranged by size: 2626 1333 1146 431 136 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3199 3322 3417 3663 669 Size: 988 281 1261 123 95 246 3079 Fragments arranged by size: 3079 1261 988 281 246 123 95 HindIII A'AGCT_T Cuts at: 748 2924 748 Size: 2176 3897 HpaI GTT'AAC Cuts at: 3199 3322 3663 3199 Size: 123 341 5609 Fragments arranged by size: 5609 341 123 KpnI G_GTAC'C Cuts at: 3398 3398 Size: 6073 MfeI C'AATT_G Cuts at: 3672 3672 Size: 6073 MluI A'CGCG_T Cuts at: 5949 5949 Size: 6073 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5187 5371 1305 Size: 3882 184 2007 Fragments arranged by size: 3882 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3104 10 Size: 54 1425 343 1272 2979 Fragments arranged by size: 2979 1425 1272 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4031 4390 4549 518 Size: 648 2865 359 159 2042 Fragments arranged by size: 2865 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2701 3489 4166 5314 5559 1606 Size: 1095 788 677 1148 245 2120 Fragments arranged by size: 2120 1148 1095 788 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4582 Fragments arranged by size: 4582 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6073 NheI G'CTAG_C Cuts at: 1052 2104 1052 Size: 1052 5021 NotI GC'GGCC_GC Cuts at: 3520 3520 Size: 6073 NspI r_CATG'y Cuts at: 1132 1229 3431 4976 1132 Size: 97 2202 1545 2229 Fragments arranged by size: 2229 2202 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6073 PciI A'CATG_T Cuts at: 4972 4972 Size: 6073 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6073 PfoI T'CCnGG_A Cuts at: 2646 2646 Size: 6073 PmeI GTTT'AAAC Cuts at: 3377 3377 Size: 6073 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4142 4174 5655 5687 4142 Size: 32 1481 32 4528 Fragments arranged by size: 4528 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6073 PsiI TTA'TAA Cuts at: 3643 3643 Size: 6073 PstI C_TGCA'G Cuts at: 830 3425 830 Size: 2595 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6073 PvuII CAG'CTG Cuts at: 2701 2701 Size: 6073 SalI G'TCGA_C Cuts at: 1936 3415 1936 Size: 1479 4594 SbfI CC_TGCA'GG Cuts at: 3425 3425 Size: 6073 ScaI AGT'ACT Cuts at: 2681 4321 6033 2681 Size: 1640 1712 2721 Fragments arranged by size: 2721 1712 1640 SfcI C'TryA_G Cuts at: 826 1045 2062 2743 3033 3421 4556 5237 5428 Size: 219 1017 681 290 388 1135 681 191 Cuts at: 5428 826 Size: 1471 Fragments arranged by size: 1471 1135 1017 681 681 388 290 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6073 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6073 SmaI CCC'GGG Cuts at: 1872 3400 1872 Size: 1528 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3002 4177 5078 5340 820 Size: 197 926 1059 1175 901 262 1553 Fragments arranged by size: 1553 1175 1059 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3864 493 Size: 3371 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6073 SphI G_CATG'C Cuts at: 1132 3431 1132 Size: 2299 3774 SspI AAT'ATT Cuts at: 5 52 2263 3091 3997 5 Size: 47 2211 828 906 2081 Fragments arranged by size: 2211 2081 906 828 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6073 StyI C'CwwG_G Cuts at: 513 1065 2004 2109 3258 3512 513 Size: 552 939 105 1149 254 3074 Fragments arranged by size: 3074 1149 939 552 254 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4100 4117 4270 4455 4900 325 Size: 1255 2520 17 153 185 445 1498 Fragments arranged by size: 2520 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2330 2679 2815 Size: 274 80 33 51 1443 353 349 136 Cuts at: 2815 4319 6031 96 Size: 1504 1712 138 Fragments arranged by size: 1712 1504 1443 353 349 274 138 136 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2341 2373 6033 6065 2341 Size: 32 3660 32 2349 Fragments arranged by size: 3660 2349 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6073 XbaI T'CTAG_A Cuts at: 3409 3409 Size: 6073 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6073 XmaI C'CCGG_G Cuts at: 1870 3398 1870 Size: 1528 4545 XmnI GAAnn'nnTTC Cuts at: 2142 2898 2995 3177 4202 2142 Size: 756 97 182 1025 4013 Fragments arranged by size: 4013 1025 756 182 97 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3881 276 Size: 53 83 186 936 2347 2468 Fragments arranged by size: 2468 2347 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI