(Circular) (Six-base) MAPSORT of: pFN21AB3002.seq Check: 799 from: 1 to: 6430 pFN21AB3002 6430 bp With 182 enzymes: SgfI * February 27, 2009 14:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3288 3773 1937 Size: 1351 485 4594 Fragments arranged by size: 4594 1351 485 Acc65I G'GTAC_C Cuts at: 3060 3751 3060 Size: 691 5739 AclI AA'CG_TT Cuts at: 1563 4557 4930 1563 Size: 2994 373 3063 Fragments arranged by size: 3063 2994 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2345 2474 3155 3319 3667 4493 5877 6145 Size: 713 129 681 164 348 826 1384 268 Cuts at: 6145 1632 Size: 1917 Fragments arranged by size: 1917 1384 826 713 681 348 268 164 129 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 5261 5261 Size: 6430 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 3608 1166 Size: 2442 3988 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6430 AlwNI CAG_nnn'CTG Cuts at: 2702 4173 5745 2702 Size: 1471 1572 3387 Fragments arranged by size: 3387 1572 1471 ApaI G_GGCC'C Cuts at: 2143 2237 2833 2143 Size: 94 596 5740 Fragments arranged by size: 5740 596 94 ApaLI G'TGCA_C Cuts at: 2727 4487 5643 2727 Size: 1760 1156 3514 Fragments arranged by size: 3514 1760 1156 ApoI r'AATT_y Cuts at: 1493 2049 3739 3965 4117 6226 1493 Size: 556 1690 226 152 2109 1697 Fragments arranged by size: 2109 1697 1690 556 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2172 2317 3755 1870 Size: 73 229 145 1438 4545 Fragments arranged by size: 4545 1438 229 145 73 AvrII C'CTAG_G Cuts at: 2090 2090 Size: 6430 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3313 3346 3313 Size: 33 6397 BamHI G'GATC_C Cuts at: 2008 3760 4127 2008 Size: 1752 367 4311 Fragments arranged by size: 4311 1752 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2471 2557 3060 3459 3465 Size: 325 217 627 684 86 503 399 6 Cuts at: 3465 3484 3751 5206 618 Size: 19 267 1455 1842 Fragments arranged by size: 1842 1455 684 627 503 399 325 267 217 86 19 6 BanII G_rGCy'C Cuts at: 1391 2132 2143 2173 2237 2287 2833 3015 3559 Size: 741 11 30 64 50 546 182 544 Cuts at: 3559 3749 1391 Size: 190 4072 Fragments arranged by size: 4072 741 546 544 190 182 64 50 30 11 BbeI G_GCGC'C Cuts at: 3469 3469 Size: 6430 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3093 3347 3673 928 Size: 971 1194 254 326 3685 Fragments arranged by size: 3685 1194 971 326 254 BbvCI CC'TCA_GC Cuts at: 3599 3599 Size: 6430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4621 4655 1903 Size: 34 2684 34 3678 Fragments arranged by size: 3678 2684 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2918 4322 5532 2918 Size: 1404 1210 3816 Fragments arranged by size: 3816 1404 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BfrBI ATG'CAT Cuts at: 2063 2063 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3454 5041 136 Size: 107 122 71 631 605 1782 1587 1525 Fragments arranged by size: 1782 1587 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6425 6425 Size: 6430 BlpI GC'TnA_GC Cuts at: 2126 2325 2348 3717 3847 2126 Size: 199 23 1369 130 4709 Fragments arranged by size: 4709 1369 199 130 23 Bme1580I G_kGCm'C Cuts at: 1790 2143 2237 2731 2833 3489 3511 4491 5647 Size: 353 94 494 102 656 22 980 1156 Cuts at: 5647 1790 Size: 2573 Fragments arranged by size: 2573 1156 980 656 494 353 102 94 22 BmgBI CAC'GTC Cuts at: 1318 3292 1318 Size: 1974 4456 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2403 3736 4161 Size: 416 53 83 399 24 986 1333 425 Cuts at: 4161 5119 442 Size: 958 1753 Fragments arranged by size: 1753 1333 986 958 425 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3034 3034 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2975 5090 1239 Size: 1736 2115 2579 Fragments arranged by size: 2579 2115 1736 Bpu10I CC'TnA_GC Cuts at: 3599 3599 Size: 6430 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2693 2722 2846 4555 5420 5718 2693 Size: 29 124 1709 865 298 3405 Fragments arranged by size: 3405 1709 865 298 124 29 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5093 882 Size: 617 3594 2219 Fragments arranged by size: 3594 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4221 493 Size: 711 3017 2702 Fragments arranged by size: 3017 2702 711 BsaBI GATnn'nnATC Cuts at: 6424 6424 Size: 6430 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3466 4237 4619 5254 Size: 53 83 186 936 1933 771 382 635 Cuts at: 5254 275 Size: 1451 Fragments arranged by size: 1933 1451 936 771 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3615 4863 5261 5535 5682 6093 3615 Size: 1248 398 274 147 411 3952 Fragments arranged by size: 3952 1248 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2651 2681 1178 Size: 30 165 30 1248 30 4927 Fragments arranged by size: 4927 1248 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2383 2496 2499 2502 2505 2526 3188 3413 Size: 1197 113 3 3 3 21 662 225 Cuts at: 3413 1186 Size: 4203 Fragments arranged by size: 4203 1197 662 225 113 21 3 3 3 BseYI C'CCAG_C Cuts at: 2307 2435 2574 2695 3035 3117 5633 6129 2307 Size: 128 139 121 340 82 2516 496 2608 Fragments arranged by size: 2608 2516 496 340 139 128 121 82 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2646 3516 1163 Size: 573 910 870 4077 Fragments arranged by size: 4077 910 870 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3880 4641 5669 1540 Size: 275 186 1879 761 1028 2301 Fragments arranged by size: 2301 1879 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2132 2326 2731 3015 3297 4491 4576 5647 2132 Size: 194 405 284 282 1194 85 1071 2915 Fragments arranged by size: 2915 1194 1071 405 284 282 194 85 BsmI GAATG_Cn' Cuts at: 3941 4034 3941 Size: 93 6337 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2132 2143 2173 2237 2287 2326 2731 Size: 399 342 11 30 64 50 39 405 Cuts at: 2731 2833 3015 3297 3489 3511 3559 3749 4491 Size: 102 182 282 192 22 48 190 742 Cuts at: 4491 4576 5647 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 342 282 192 190 182 102 85 64 50 48 39 30 22 11 BspEI T'CCGG_A Cuts at: 3615 6093 3615 Size: 2478 3952 BspHI T'CATG_A Cuts at: 4319 6049 4319 Size: 1730 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3721 3785 844 Size: 2877 64 3489 Fragments arranged by size: 3489 2877 64 BsrBI CCG'CTC Cuts at: 1394 1823 2961 3546 3721 4317 1394 Size: 429 1138 585 175 596 3507 Fragments arranged by size: 3507 1138 596 585 429 175 BsrDI GCAATG_nn' Cuts at: 65 1248 4925 5099 65 Size: 1183 3677 174 1396 Fragments arranged by size: 3677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3315 3435 3462 3524 5074 5261 1700 Size: 110 1505 120 27 62 1550 187 2869 Fragments arranged by size: 2869 1550 1505 187 120 110 62 27 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 4490 5502 4490 Size: 1012 5418 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6430 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6430 BstYI r'GATC_y Cuts at: 1972 2008 2949 3760 4127 4512 4529 5981 6067 Size: 36 941 811 367 385 17 1452 86 Cuts at: 6067 6079 6425 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 941 811 385 367 346 86 36 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3625 513 Size: 552 939 1621 3318 Fragments arranged by size: 3318 1621 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 3603 3946 4740 4760 3603 Size: 343 794 20 5273 Fragments arranged by size: 5273 794 343 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2653 2688 529 Size: 35 2089 35 4271 Fragments arranged by size: 4271 2089 35 35 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DraIII CAC_nnn'GTG Cuts at: 2254 2254 Size: 6430 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2212 2653 2793 Size: 54 1425 50 275 18 382 441 140 Cuts at: 2793 3313 3561 3646 3877 4766 6395 8 Size: 520 248 85 231 889 1629 43 Fragments arranged by size: 1629 1425 889 520 441 382 275 248 231 140 85 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3877 1537 Size: 275 2065 4090 Fragments arranged by size: 4090 2065 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2112 3020 4360 1353 Size: 741 18 908 1340 3423 Fragments arranged by size: 3423 1340 908 741 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2279 5017 5391 5537 387 Size: 1064 138 690 2738 374 146 1280 Fragments arranged by size: 2738 1280 1064 690 374 146 138 EcoICRI GAG'CTC Cuts at: 2130 3013 2130 Size: 883 5547 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2345 2474 2975 3155 3319 3667 4493 Size: 393 713 129 501 180 164 348 826 Cuts at: 4493 5090 5877 6145 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 826 787 713 597 501 393 348 268 180 164 129 EcoO109I rG'GnC_Cy Cuts at: 2233 2482 2829 2830 3641 2233 Size: 249 347 1 811 5022 Fragments arranged by size: 5022 811 347 249 1 EcoRI G'AATT_C Cuts at: 3739 4117 3739 Size: 378 6052 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3697 3697 Size: 6430 FspI TGC'GCA Cuts at: 2153 4936 2153 Size: 2783 3647 FspAI rTGC'GCAy Cuts at: 2153 2153 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 3469 5577 1678 Size: 1791 2108 2531 Fragments arranged by size: 2531 2108 1791 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2051 2083 2382 2414 3302 Size: 32 135 32 479 32 299 32 888 Cuts at: 3302 3334 5071 5103 5145 5177 1373 Size: 32 1737 32 42 32 2626 Fragments arranged by size: 2626 1737 888 479 299 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3289 3774 4020 669 Size: 988 281 1351 485 246 3079 Fragments arranged by size: 3079 1351 988 485 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KasI G'GCGC_C Cuts at: 3465 3465 Size: 6430 KpnI G_GTAC'C Cuts at: 3064 3755 3064 Size: 691 5739 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3045 5544 5728 1305 Size: 1740 2499 184 2007 Fragments arranged by size: 2499 2007 1740 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2655 2795 3563 10 Size: 54 1425 343 823 140 768 2877 Fragments arranged by size: 2877 1425 823 768 343 140 54 MslI CAynn'nnrTG Cuts at: 518 1166 2058 2255 2988 3144 3309 3479 3482 Size: 648 892 197 733 156 165 170 3 Cuts at: 3482 3608 4388 4747 4906 518 Size: 126 780 359 159 2042 Fragments arranged by size: 2042 892 780 733 648 359 197 170 165 159 156 126 3 MspA1I CmG'CkG Cuts at: 1606 2311 2329 2630 3846 4523 5671 5916 1606 Size: 705 18 301 1216 677 1148 245 2120 Fragments arranged by size: 2120 1216 1148 705 677 301 245 18 NaeI GCC'GGC Cuts at: 3437 3437 Size: 6430 NarI GG'CG_CC Cuts at: 3466 3466 Size: 6430 NcoI C'CATG_G Cuts at: 513 1065 2004 3625 513 Size: 552 939 1621 3318 Fragments arranged by size: 3318 1621 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NgoMIV G'CCGG_C Cuts at: 3435 3435 Size: 6430 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NsiI A_TGCA'T Cuts at: 2065 2065 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 3249 3596 3788 5333 1132 Size: 97 2020 347 192 1545 2229 Fragments arranged by size: 2229 2020 1545 347 192 97 PasI CC'CwG_GG Cuts at: 1348 2013 2230 2833 2912 3168 3443 1348 Size: 665 217 603 79 256 275 4335 Fragments arranged by size: 4335 665 603 275 256 217 79 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 3027 1108 Size: 1919 4511 PfoI T'CCnGG_A Cuts at: 2709 2952 3153 2709 Size: 243 201 5986 Fragments arranged by size: 5986 243 201 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3582 3614 4499 4531 6012 6044 3582 Size: 32 885 32 1481 32 3968 Fragments arranged by size: 3968 1481 885 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2139 2233 2829 2139 Size: 94 596 5740 Fragments arranged by size: 5740 596 94 PstI C_TGCA'G Cuts at: 830 2906 3782 830 Size: 2076 876 3478 Fragments arranged by size: 3478 2076 876 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2311 2329 2630 2311 Size: 18 301 6111 Fragments arranged by size: 6111 301 18 SacI G_AGCT'C Cuts at: 2132 3015 2132 Size: 883 5547 SalI G'TCGA_C Cuts at: 1936 3287 3772 1936 Size: 1351 485 4594 Fragments arranged by size: 4594 1351 485 SapI GCTCTTCn'nnn_ Cuts at: 3020 3020 Size: 6430 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 2076 3422 4678 6390 2076 Size: 1346 1256 1712 2116 Fragments arranged by size: 2116 1712 1346 1256 SfcI C'TryA_G Cuts at: 826 1045 2803 2902 3180 3490 3778 4913 5594 Size: 219 1758 99 278 310 288 1135 681 Cuts at: 5594 5785 826 Size: 191 1471 Fragments arranged by size: 1758 1471 1135 681 310 288 278 219 191 99 SfoI GGC'GCC Cuts at: 3467 3467 Size: 6430 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 3462 1700 Size: 1762 4668 SmaI CCC'GGG Cuts at: 1872 2174 2319 3757 1872 Size: 302 145 1438 4545 Fragments arranged by size: 4545 1438 302 145 SmlI C'TyrA_G Cuts at: 820 1017 1943 2672 2737 2861 4534 5435 5697 Size: 197 926 729 65 124 1673 901 262 Cuts at: 5697 820 Size: 1553 Fragments arranged by size: 1673 1553 926 901 729 262 197 124 65 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 3788 1132 Size: 2656 3774 SrfI GCCC'GGGC Cuts at: 2174 2174 Size: 6430 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 2527 2900 3104 3218 3696 1517 Size: 1010 373 204 114 478 4251 Fragments arranged by size: 4251 1010 478 373 204 114 StyI C'CwwG_G Cuts at: 513 1065 2004 2090 2844 3570 3625 3869 513 Size: 552 939 86 754 726 55 244 3074 Fragments arranged by size: 3074 939 754 726 552 244 86 55 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2489 2582 3321 3638 4457 4474 4627 Size: 1255 909 93 739 317 819 17 153 Cuts at: 4627 4812 5257 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 909 819 739 445 317 185 153 93 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2074 3420 4676 Size: 274 80 33 51 1443 97 1346 1256 Cuts at: 4676 6388 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1346 1256 274 138 97 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6390 6422 6390 Size: 32 6398 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6430 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2416 3401 3556 2416 Size: 985 155 5290 Fragments arranged by size: 5290 985 155 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6430 XmaI C'CCGG_G Cuts at: 1870 2172 2317 3755 1870 Size: 302 145 1438 4545 Fragments arranged by size: 4545 1438 302 145 XmnI GAAnn'nnTTC Cuts at: 4559 4559 Size: 6430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FseI NruI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SwaI