(Circular) (Six-base) MAPSORT of: pFN21AB0179.seq Check: 3852 from: 1 to: 8365 pFN21AB0179 8365 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5386 5386 Size: 8365 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6175 278 Size: 53 83 186 936 4639 2468 Fragments arranged by size: 4639 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5708 1937 Size: 3771 4594 Acc65I G'GTAC_C Cuts at: 5686 5686 Size: 8365 AclI AA'CG_TT Cuts at: 1563 6492 6865 1563 Size: 4929 373 3063 Fragments arranged by size: 4929 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2369 3162 3734 5139 6428 7812 8080 1632 Size: 737 793 572 1405 1289 1384 268 1917 Fragments arranged by size: 1917 1405 1384 1289 793 737 572 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8168 AflIII A'CryG_T Cuts at: 1203 7264 8241 1203 Size: 6061 977 1327 Fragments arranged by size: 6061 1327 977 AgeI A'CCGG_T Cuts at: 7196 7196 Size: 8365 AhdI GACnn_n'nnGTC Cuts at: 7094 7094 Size: 8365 AleI CACnn'nnGTG Cuts at: 1166 4088 4751 1166 Size: 2922 663 4780 Fragments arranged by size: 4780 2922 663 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2511 1184 Size: 1327 7038 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4398 4430 4398 Size: 32 8333 AlwNI CAG_nnn'CTG Cuts at: 3754 3779 3808 6108 7680 3754 Size: 25 29 2300 1572 4439 Fragments arranged by size: 4439 2300 1572 29 25 ApaI G_GGCC'C Cuts at: 4903 5230 4903 Size: 327 8038 ApaLI G'TGCA_C Cuts at: 2888 6422 7578 2888 Size: 3534 1156 3675 Fragments arranged by size: 3675 3534 1156 ApoI r'AATT_y Cuts at: 1493 2156 2231 3248 5105 5674 5900 6052 8161 Size: 663 75 1017 1857 569 226 152 2109 Cuts at: 8161 1493 Size: 1697 Fragments arranged by size: 2109 1857 1697 1017 663 569 226 152 75 AseI AT'TA_AT Cuts at: 160 1032 6919 160 Size: 872 5887 1606 Fragments arranged by size: 5887 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8365 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2640 5646 5690 1870 Size: 73 135 562 3006 44 4545 Fragments arranged by size: 4545 3006 562 135 73 44 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2066 2099 5013 5046 2066 Size: 33 2914 33 5385 Fragments arranged by size: 5385 2914 33 33 BamHI G'GATC_C Cuts at: 5695 6062 5695 Size: 367 7998 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5686 7141 618 Size: 325 217 627 3899 1455 1842 Fragments arranged by size: 3899 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2790 2904 3386 4903 5230 5684 1391 Size: 1399 114 482 1517 327 454 4072 Fragments arranged by size: 4072 1517 1399 482 454 327 114 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4330 5047 928 Size: 971 2431 717 4246 Fragments arranged by size: 4246 2431 971 717 BbvCI CC'TCA_GC Cuts at: 4153 4153 Size: 8365 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3858 3892 4518 4552 6556 6590 1903 Size: 34 1921 34 626 34 2004 34 3678 Fragments arranged by size: 3678 2004 1921 626 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5041 6257 7467 5041 Size: 1216 1210 5939 Fragments arranged by size: 5939 1216 1210 BclI T'GATC_A Cuts at: 1548 2163 4866 1548 Size: 615 2703 5047 Fragments arranged by size: 5047 2703 615 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6976 136 Size: 107 122 71 631 605 5304 1525 Fragments arranged by size: 5304 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3037 8360 3037 Size: 5323 3042 BlpI GC'TnA_GC Cuts at: 5570 5782 5570 Size: 212 8153 Bme1580I G_kGCm'C Cuts at: 1790 2892 3360 4903 5230 5425 6426 7582 1790 Size: 1102 468 1543 327 195 1001 1156 2573 Fragments arranged by size: 2573 1543 1156 1102 1001 468 327 195 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8365 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2357 2994 3538 Size: 416 53 83 399 24 940 637 544 Cuts at: 3538 3796 4430 4449 4904 6096 7054 442 Size: 258 634 19 455 1192 958 1753 Fragments arranged by size: 1753 1192 958 940 637 634 544 455 416 399 258 83 53 24 19 BmtI G_CTAG'C Cuts at: 1056 5144 1056 Size: 4088 4277 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3106 3172 5661 3106 Size: 66 2489 5810 Fragments arranged by size: 5810 2489 66 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2271 2763 3051 4979 5656 7025 1239 Size: 1032 492 288 1928 677 1369 2579 Fragments arranged by size: 2579 1928 1369 1032 677 492 288 Bpu10I CC'TnA_GC Cuts at: 3002 3916 4153 4650 3002 Size: 914 237 497 6717 Fragments arranged by size: 6717 914 497 237 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3335 3701 4784 6490 7355 7653 3335 Size: 366 1083 1706 865 298 4047 Fragments arranged by size: 4047 1706 1083 865 366 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2586 3068 3080 5557 7028 882 Size: 617 1087 482 12 2477 1471 2219 Fragments arranged by size: 2477 2219 1471 1087 617 482 12 BsaAI yAC'GTr Cuts at: 493 1204 6156 493 Size: 711 4952 2702 Fragments arranged by size: 4952 2702 711 BsaBI GATnn'nnATC Cuts at: 3018 3674 8359 3018 Size: 656 4685 3024 Fragments arranged by size: 4685 3024 656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4634 6172 6554 7189 Size: 53 83 186 936 3101 1538 382 635 Cuts at: 7189 275 Size: 1451 Fragments arranged by size: 3101 1538 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2377 2706 5376 6798 7196 7470 7617 8028 2377 Size: 329 2670 1422 398 274 147 411 2714 Fragments arranged by size: 2714 2670 1422 411 398 329 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2671 2677 2701 2707 4871 Size: 30 165 30 1268 6 24 6 2164 Cuts at: 4871 4901 5159 5189 1178 Size: 30 258 30 4354 Fragments arranged by size: 4354 2164 1268 258 165 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2766 3098 3914 1186 Size: 1580 332 816 5637 Fragments arranged by size: 5637 1580 816 332 BseYI C'CCAG_C Cuts at: 2465 3480 3852 4483 4676 5536 7568 8064 2465 Size: 1015 372 631 193 860 2032 496 2766 Fragments arranged by size: 2766 2032 1015 860 631 496 372 193 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2247 2870 3940 5066 5333 1163 Size: 573 511 623 1070 1126 267 4195 Fragments arranged by size: 4195 1126 1070 623 573 511 267 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5815 6576 7604 1540 Size: 275 186 3814 761 1028 2301 Fragments arranged by size: 3814 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2892 2904 3139 4095 5472 6426 6511 7582 2892 Size: 12 235 956 1377 954 85 1071 3675 Fragments arranged by size: 3675 1377 1071 956 954 235 85 12 BsmI GAATG_Cn' Cuts at: 3276 5876 5969 3276 Size: 2600 93 5672 Fragments arranged by size: 5672 2600 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2270 2270 Size: 8365 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2790 2892 2904 3139 3360 3386 4095 Size: 399 1000 102 12 235 221 26 709 Cuts at: 4095 4903 5230 5425 5472 5684 6426 6511 7582 Size: 808 327 195 47 212 742 85 1071 Cuts at: 7582 1391 Size: 2174 Fragments arranged by size: 2174 1071 1000 808 742 709 399 327 235 221 212 195 102 85 47 26 12 BspEI T'CCGG_A Cuts at: 8028 8028 Size: 8365 BspHI T'CATG_A Cuts at: 6254 7984 6254 Size: 1730 6635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5386 5720 844 Size: 4542 334 3489 Fragments arranged by size: 4542 3489 334 BsrBI CCG'CTC Cuts at: 1394 1823 2619 2734 6252 1394 Size: 429 796 115 3518 3507 Fragments arranged by size: 3518 3507 796 429 115 BsrDI GCAATG_nn' Cuts at: 65 1248 3438 6860 7034 65 Size: 1183 2190 3422 174 1396 Fragments arranged by size: 3422 2190 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7009 7196 1700 Size: 110 5199 187 2869 Fragments arranged by size: 5199 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 4245 4710 4887 5148 5607 96 Size: 4149 465 177 261 459 2854 Fragments arranged by size: 4149 2854 465 459 261 177 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8365 BssSI C'ACGA_G Cuts at: 4625 6425 7437 4625 Size: 1800 1012 5553 Fragments arranged by size: 5553 1800 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8365 BstBI TT'CG_AA Cuts at: 7169 7169 Size: 8365 BstEII G'GTnAC_C Cuts at: 1184 5372 1184 Size: 4188 4177 BstXI CCAn_nnnn'nTGG Cuts at: 2011 3201 3865 2011 Size: 1190 664 6511 Fragments arranged by size: 6511 1190 664 BstYI r'GATC_y Cuts at: 1972 2311 3037 3750 3776 4377 5695 6062 6447 Size: 339 726 713 26 601 1318 367 385 Cuts at: 6447 6464 7916 8002 8014 8360 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1318 726 713 601 385 367 346 339 86 26 17 12 Bsu36I CC'TnA_GG Cuts at: 3285 3285 Size: 8365 BtgI C'CryG_G Cuts at: 513 1065 2004 2022 2058 3510 4806 4875 5183 Size: 552 939 18 36 1452 1296 69 308 Cuts at: 5183 513 Size: 3695 Fragments arranged by size: 3695 1452 1296 939 552 308 69 36 18 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4521 487 Size: 683 56 3295 4331 Fragments arranged by size: 4331 3295 683 56 BtsI GCAGTG_nn' Cuts at: 4176 5014 5335 5881 6675 6695 4176 Size: 838 321 546 794 20 5846 Fragments arranged by size: 5846 838 794 546 321 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8365 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8330 DraI TTT'AAA Cuts at: 5669 5669 Size: 8365 DraIII CAC_nnn'GTG Cuts at: 4325 4325 Size: 8365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3670 7372 809 Size: 507 2354 3702 1802 Fragments arranged by size: 3702 2354 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2922 3450 3882 Size: 54 1425 50 275 18 1092 528 432 Cuts at: 3882 5812 6701 8330 8 Size: 1930 889 1629 43 Fragments arranged by size: 1930 1629 1425 1092 889 528 432 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5812 1537 Size: 275 4000 4090 Fragments arranged by size: 4090 4000 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2363 2778 3261 3330 3856 4455 5432 6295 Size: 1010 415 483 69 526 599 977 863 Cuts at: 6295 1353 Size: 3423 Fragments arranged by size: 3423 1010 977 863 599 526 483 415 69 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3827 6952 7326 7472 387 Size: 1064 138 2238 3125 374 146 1280 Fragments arranged by size: 3125 2238 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2902 2902 Size: 8365 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2271 2369 2763 3051 3162 3734 4979 Size: 393 639 98 394 288 111 572 1245 Cuts at: 4979 5139 5656 6428 7025 7812 8080 1239 Size: 160 517 772 597 787 268 1524 Fragments arranged by size: 1524 1245 787 772 639 597 572 517 394 393 288 268 160 111 98 EcoNI CCTnn'n_nnAGG Cuts at: 3812 3812 Size: 8365 EcoO109I rG'GnC_Cy Cuts at: 2054 2146 2637 3310 4406 4899 4900 4915 2054 Size: 92 491 673 1096 493 1 15 5504 Fragments arranged by size: 5504 1096 673 493 491 92 15 1 EcoRI G'AATT_C Cuts at: 5674 6052 5674 Size: 378 7987 EcoRV GAT'ATC Cuts at: 2669 4330 2669 Size: 1661 6704 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2374 3646 2374 Size: 1272 7093 FspI TGC'GCA Cuts at: 6871 6871 Size: 8365 HaeII r_GCGC'y Cuts at: 1678 3175 7512 1678 Size: 1497 4337 2531 Fragments arranged by size: 4337 2531 1497 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2072 2104 2671 Size: 32 135 32 450 32 18 32 567 Cuts at: 2671 2677 2703 2709 3761 3793 4255 4287 4811 Size: 6 26 6 1052 32 462 32 524 Cuts at: 4811 4843 7006 7038 7080 7112 1373 Size: 32 2163 32 42 32 2626 Fragments arranged by size: 2626 2163 1052 567 524 462 450 135 42 32 32 32 32 32 32 32 32 32 26 18 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3535 5709 5955 669 Size: 988 281 1597 2174 246 3079 Fragments arranged by size: 3079 2174 1597 988 281 246 HindIII A'AGCT_T Cuts at: 748 2454 748 Size: 1706 6659 HpaI GTT'AAC Cuts at: 5955 5955 Size: 8365 KpnI G_GTAC'C Cuts at: 5690 5690 Size: 8365 MfeI C'AATT_G Cuts at: 3722 5964 3722 Size: 2242 6123 MluI A'CGCG_T Cuts at: 8241 8241 Size: 8365 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2025 2385 4035 5202 5680 7479 7663 1305 Size: 720 360 1650 1167 478 1799 184 2007 Fragments arranged by size: 2007 1799 1650 1167 720 478 360 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2924 3452 3884 10 Size: 54 1425 343 1092 528 432 4491 Fragments arranged by size: 4491 1425 1092 528 432 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3377 4086 4088 4751 4874 4991 Size: 648 843 1368 709 2 663 123 117 Cuts at: 4991 6323 6682 6841 518 Size: 1332 359 159 2042 Fragments arranged by size: 2042 1368 1332 843 709 663 648 359 159 123 117 2 MspA1I CmG'CkG Cuts at: 1606 2060 2854 3484 4885 5781 6458 7606 7851 Size: 454 794 630 1401 896 677 1148 245 Cuts at: 7851 1606 Size: 2120 Fragments arranged by size: 2120 1401 1148 896 794 677 630 454 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2022 3510 4806 4875 5183 513 Size: 552 939 18 1488 1296 69 308 3695 Fragments arranged by size: 3695 1488 1296 939 552 308 69 18 NdeI CA'TA_TG Cuts at: 387 387 Size: 8365 NheI G'CTAG_C Cuts at: 1052 5140 1052 Size: 4088 4277 NotI GC'GGCC_GC Cuts at: 5812 5812 Size: 8365 NspI r_CATG'y Cuts at: 1132 1229 2765 3629 5491 5723 7268 1132 Size: 97 1536 864 1862 232 1545 2229 Fragments arranged by size: 2229 1862 1545 1536 864 232 97 PasI CC'CwG_GG Cuts at: 1348 4402 4691 1348 Size: 3054 289 5022 Fragments arranged by size: 5022 3054 289 PciI A'CATG_T Cuts at: 7264 7264 Size: 8365 PflMI CCAn_nnn'nTGG Cuts at: 1108 3808 3928 4627 5184 5398 5464 5634 1108 Size: 2700 120 699 557 214 66 170 3839 Fragments arranged by size: 3839 2700 699 557 214 170 120 66 PfoI T'CCnGG_A Cuts at: 2633 2675 5156 2633 Size: 42 2481 5842 Fragments arranged by size: 5842 2481 42 PmeI GTTT'AAAC Cuts at: 5669 5669 Size: 8365 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6434 6466 7947 7979 6434 Size: 32 1481 32 6820 Fragments arranged by size: 6820 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2146 2637 3310 4406 2146 Size: 491 673 1096 6105 Fragments arranged by size: 6105 1096 673 491 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8365 PsiI TTA'TAA Cuts at: 2808 5935 2808 Size: 3127 5238 PspOMI G'GGCC_C Cuts at: 4899 5226 4899 Size: 327 8038 PspXI vC'TCGA_Gb Cuts at: 2078 2078 Size: 8365 PstI C_TGCA'G Cuts at: 830 5009 5330 5717 830 Size: 4179 321 387 3478 Fragments arranged by size: 4179 3478 387 321 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8365 PvuII CAG'CTG Cuts at: 2854 4885 2854 Size: 2031 6334 SacI G_AGCT'C Cuts at: 2904 2904 Size: 8365 SacII CC_GC'GG Cuts at: 2061 2061 Size: 8365 SalI G'TCGA_C Cuts at: 1936 5707 1936 Size: 3771 4594 SapI GCTCTTCn'nnn_ Cuts at: 2778 3330 4455 2778 Size: 552 1125 6688 Fragments arranged by size: 6688 1125 552 SbfI CC_TGCA'GG Cuts at: 5717 5717 Size: 8365 ScaI AGT'ACT Cuts at: 6613 8325 6613 Size: 1712 6653 SexAI A'CCwGG_T Cuts at: 4321 4321 Size: 8365 SfcI C'TryA_G Cuts at: 826 1045 2016 5005 5059 5326 5713 6848 7529 Size: 219 971 2989 54 267 387 1135 681 Cuts at: 7529 7720 826 Size: 191 1471 Fragments arranged by size: 2989 1471 1135 971 681 387 267 219 191 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8365 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8365 SmaI CCC'GGG Cuts at: 1872 2642 5692 1872 Size: 770 3050 4545 Fragments arranged by size: 4545 3050 770 SmlI C'TyrA_G Cuts at: 820 1017 1943 2078 3314 3680 4799 5646 6469 Size: 197 926 135 1236 366 1119 847 823 Cuts at: 6469 7370 7632 820 Size: 901 262 1553 Fragments arranged by size: 1553 1236 1119 926 901 847 823 366 262 197 135 SnaBI TAC'GTA Cuts at: 493 6156 493 Size: 5663 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8365 SphI G_CATG'C Cuts at: 1132 3629 5723 1132 Size: 2497 2094 3774 Fragments arranged by size: 3774 2497 2094 SspI AAT'ATT Cuts at: 5 52 6289 5 Size: 47 6237 2081 Fragments arranged by size: 6237 2081 47 StuI AGG'CCT Cuts at: 1517 2883 1517 Size: 1366 6999 StyI C'CwwG_G Cuts at: 513 1065 2004 2022 3447 3510 4161 4806 4875 Size: 552 939 18 1425 63 651 645 69 Cuts at: 4875 5067 5183 5478 5804 513 Size: 192 116 295 326 3074 Fragments arranged by size: 3074 1425 939 651 645 552 326 295 192 116 69 63 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2661 3815 3834 5437 6392 6409 6562 Size: 1255 1081 1154 19 1603 955 17 153 Cuts at: 6562 6747 7192 325 Size: 185 445 1498 Fragments arranged by size: 1603 1498 1255 1154 1081 955 445 185 153 19 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3981 4245 4440 Size: 274 80 33 51 1443 2004 264 195 Cuts at: 4440 4710 4887 5148 5607 6611 8323 96 Size: 270 177 261 459 1004 1712 138 Fragments arranged by size: 2004 1712 1443 1004 459 274 270 264 261 195 177 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8325 8357 8325 Size: 32 8333 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8365 XbaI T'CTAG_A Cuts at: 2663 5701 2663 Size: 3038 5327 XhoI C'TCGA_G Cuts at: 1943 2078 5646 1943 Size: 135 3568 4662 Fragments arranged by size: 4662 3568 135 XmaI C'CCGG_G Cuts at: 1870 2640 5690 1870 Size: 770 3050 4545 Fragments arranged by size: 4545 3050 770 XmnI GAAnn'nnTTC Cuts at: 3249 5054 6494 3249 Size: 1805 1440 5120 Fragments arranged by size: 5120 1805 1440 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6173 276 Size: 53 83 186 936 4639 2468 Fragments arranged by size: 4639 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SanDI SfiI SfoI SrfI SwaI XcmI