(Circular) (Six-base) MAPSORT of: pFN21AA1902.seq Check: 5446 from: 1 to: 7984 pFN21AA1902 7984 bp With 182 enzymes: SgfI * January 16, 2009 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3030 3030 Size: 7984 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5794 278 Size: 53 83 186 936 4258 2468 Fragments arranged by size: 4258 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3401 5327 1937 Size: 1464 1926 4594 Fragments arranged by size: 4594 1926 1464 Acc65I G'GTAC_C Cuts at: 5305 5305 Size: 7984 AclI AA'CG_TT Cuts at: 1563 6111 6484 1563 Size: 4548 373 3063 Fragments arranged by size: 4548 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3929 4526 4805 4838 6047 7431 7699 1632 Size: 2297 597 279 33 1209 1384 268 1917 Fragments arranged by size: 2297 1917 1384 1209 597 279 268 33 AfeI AGC'GCT Cuts at: 2900 2900 Size: 7984 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7787 AflIII A'CryG_T Cuts at: 1203 5131 6883 7860 1203 Size: 3928 1752 977 1327 Fragments arranged by size: 3928 1752 1327 977 AgeI A'CCGG_T Cuts at: 6815 6815 Size: 7984 AhdI GACnn_n'nnGTC Cuts at: 6713 6713 Size: 7984 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7984 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4412 4851 1184 Size: 3228 439 4317 Fragments arranged by size: 4317 3228 439 AlwNI CAG_nnn'CTG Cuts at: 3737 4399 4433 5727 7299 3737 Size: 662 34 1294 1572 4422 Fragments arranged by size: 4422 1572 1294 662 34 ApaLI G'TGCA_C Cuts at: 6041 7197 6041 Size: 1156 6828 ApoI r'AATT_y Cuts at: 1493 2288 2337 2375 2635 3007 3032 3450 3960 Size: 795 49 38 260 372 25 418 510 Cuts at: 3960 3986 4467 5293 5519 5671 7780 1493 Size: 26 481 826 226 152 2109 1697 Fragments arranged by size: 2109 1697 826 795 510 481 418 372 260 226 152 49 38 26 25 AseI AT'TA_AT Cuts at: 160 1032 2976 6538 160 Size: 872 1944 3562 1606 Fragments arranged by size: 3562 1944 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7984 AvaI C'yCGr_G Cuts at: 1870 1943 5309 1870 Size: 73 3366 4545 Fragments arranged by size: 4545 3366 73 BamHI G'GATC_C Cuts at: 2266 5044 5314 5681 2266 Size: 2778 270 367 4569 Fragments arranged by size: 4569 2778 367 270 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5153 5267 5305 6760 618 Size: 325 217 627 3366 114 38 1455 1842 Fragments arranged by size: 3366 1842 1455 627 325 217 114 38 BanII G_rGCy'C Cuts at: 1391 2486 3145 4725 5303 1391 Size: 1095 659 1580 578 4072 Fragments arranged by size: 4072 1580 1095 659 578 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2590 2940 3928 4190 4906 5113 928 Size: 971 691 350 988 262 716 207 3799 Fragments arranged by size: 3799 988 971 716 691 350 262 207 BbvCI CC'TCA_GC Cuts at: 4976 4976 Size: 7984 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6175 6209 1903 Size: 34 4238 34 3678 Fragments arranged by size: 4238 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5876 7086 5876 Size: 1210 6774 BclI T'GATC_A Cuts at: 1548 2200 5242 1548 Size: 652 3042 4290 Fragments arranged by size: 4290 3042 652 BfrBI ATG'CAT Cuts at: 4765 4765 Size: 7984 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6595 136 Size: 107 122 71 631 605 4923 1525 Fragments arranged by size: 4923 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4552 5176 7979 4552 Size: 624 2803 4557 Fragments arranged by size: 4557 2803 624 BlpI GC'TnA_GC Cuts at: 5401 5401 Size: 7984 Bme1580I G_kGCm'C Cuts at: 1790 6045 7201 1790 Size: 4255 1156 2573 Fragments arranged by size: 4255 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7984 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3907 5715 6673 Size: 416 53 83 399 24 2490 1808 958 Cuts at: 6673 442 Size: 1753 Fragments arranged by size: 2490 1808 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7984 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2117 2527 3986 4493 4709 6644 1239 Size: 878 410 1459 507 216 1935 2579 Fragments arranged by size: 2579 1935 1459 878 507 410 216 Bpu10I CC'TnA_GC Cuts at: 3766 4976 3766 Size: 1210 6774 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3327 4445 4715 6109 6974 7272 3327 Size: 1118 270 1394 865 298 4039 Fragments arranged by size: 4039 1394 1118 865 298 270 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6647 882 Size: 617 5148 2219 Fragments arranged by size: 5148 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2690 5775 493 Size: 711 1486 3085 2702 Fragments arranged by size: 3085 2702 1486 711 BsaBI GATnn'nnATC Cuts at: 4302 4355 7978 4302 Size: 53 3623 4308 Fragments arranged by size: 4308 3623 53 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5791 6173 6808 275 Size: 53 83 186 936 4258 382 635 1451 Fragments arranged by size: 4258 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4932 6417 6815 7089 7236 7647 4932 Size: 1485 398 274 147 411 5269 Fragments arranged by size: 5269 1485 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2585 1178 Size: 30 165 30 1152 30 6577 Fragments arranged by size: 6577 1152 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 3579 3675 3684 3687 3711 3714 3750 Size: 928 1465 96 9 3 24 3 36 Cuts at: 3750 4395 4533 5230 1186 Size: 645 138 697 3940 Fragments arranged by size: 3940 1465 928 697 645 138 96 36 24 9 3 3 BseYI C'CCAG_C Cuts at: 7187 7683 7187 Size: 496 7488 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2513 2577 3771 1163 Size: 573 777 64 1194 5376 Fragments arranged by size: 5376 1194 777 573 64 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5434 6195 7223 1540 Size: 275 186 3433 761 1028 2301 Fragments arranged by size: 3433 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2486 3145 4725 6045 6130 7201 2486 Size: 659 1580 1320 85 1071 3269 Fragments arranged by size: 3269 1580 1320 1071 659 85 BsmI GAATG_Cn' Cuts at: 3175 3888 4214 4389 5495 5588 3175 Size: 713 326 175 1106 93 5571 Fragments arranged by size: 5571 1106 713 326 175 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2486 3145 4725 5303 6045 6130 7201 Size: 399 696 659 1580 578 742 85 1071 Cuts at: 7201 1391 Size: 2174 Fragments arranged by size: 2174 1580 1071 742 696 659 578 399 85 BspEI T'CCGG_A Cuts at: 7647 7647 Size: 7984 BspHI T'CATG_A Cuts at: 2697 3421 4311 5873 7603 2697 Size: 724 890 1562 1730 3078 Fragments arranged by size: 3078 1730 1562 890 724 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2535 2553 3030 3524 4030 5339 844 Size: 1691 18 477 494 506 1309 3489 Fragments arranged by size: 3489 1691 1309 506 494 477 18 BsrBI CCG'CTC Cuts at: 1394 1823 2577 4267 5871 1394 Size: 429 754 1690 1604 3507 Fragments arranged by size: 3507 1690 1604 754 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2609 6479 6653 65 Size: 1183 1361 3870 174 1396 Fragments arranged by size: 3870 1396 1361 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6628 6815 1700 Size: 110 4818 187 2869 Fragments arranged by size: 4818 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7984 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7984 BssSI C'ACGA_G Cuts at: 6044 7056 6044 Size: 1012 6972 BstAPI GCAn_nnn'nTGC Cuts at: 794 2059 3286 4853 794 Size: 1265 1227 1567 3925 Fragments arranged by size: 3925 1567 1265 1227 BstBI TT'CG_AA Cuts at: 6788 6788 Size: 7984 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7984 BstXI CCAn_nnnn'nTGG Cuts at: 3265 3265 Size: 7984 BstYI r'GATC_y Cuts at: 1972 2266 3105 4552 5044 5176 5314 5681 6066 Size: 294 839 1447 492 132 138 367 385 Cuts at: 6066 6083 7535 7621 7633 7979 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1447 839 492 385 367 346 294 138 132 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3571 3571 Size: 7984 BtgI C'CryG_G Cuts at: 513 1065 2004 3513 513 Size: 552 939 1509 4984 Fragments arranged by size: 4984 1509 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7245 Fragments arranged by size: 7245 683 56 BtsI GCAGTG_nn' Cuts at: 2594 3599 4187 5500 6294 6314 2594 Size: 1005 588 1313 794 20 4264 Fragments arranged by size: 4264 1313 1005 794 588 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7984 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7949 DraI TTT'AAA Cuts at: 2875 3975 4537 5288 2875 Size: 1100 562 751 5571 Fragments arranged by size: 5571 1100 751 562 DraIII CAC_nnn'GTG Cuts at: 2059 4639 2059 Size: 2580 5404 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6991 809 Size: 507 5675 1802 Fragments arranged by size: 5675 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5431 6320 7949 Size: 54 1425 50 275 18 3601 889 1629 Cuts at: 7949 8 Size: 43 Fragments arranged by size: 3601 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5431 1537 Size: 275 3619 4090 Fragments arranged by size: 4090 3619 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4956 5914 1353 Size: 3603 958 3423 Fragments arranged by size: 3603 3423 958 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6571 6945 7091 387 Size: 1064 138 4982 374 146 1280 Fragments arranged by size: 4982 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2484 3143 4723 2484 Size: 659 1580 5745 Fragments arranged by size: 5745 1580 659 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2117 2527 3929 3986 4493 4526 4709 Size: 393 485 410 1402 57 507 33 183 Cuts at: 4709 4805 4838 6047 6644 7431 7699 1239 Size: 96 33 1209 597 787 268 1524 Fragments arranged by size: 1524 1402 1209 787 597 507 485 410 393 268 183 96 57 33 33 EcoNI CCTnn'n_nnAGG Cuts at: 2509 3181 3729 2509 Size: 672 548 6764 Fragments arranged by size: 6764 672 548 EcoO109I rG'GnC_Cy Cuts at: 2524 3548 3575 3734 4019 2524 Size: 1024 27 159 285 6489 Fragments arranged by size: 6489 1024 285 159 27 EcoRI G'AATT_C Cuts at: 3960 5293 5671 3960 Size: 1333 378 6273 Fragments arranged by size: 6273 1333 378 EcoRV GAT'ATC Cuts at: 3475 3475 Size: 7984 FspI TGC'GCA Cuts at: 6490 6490 Size: 7984 HaeII r_GCGC'y Cuts at: 1678 2902 5236 7131 1678 Size: 1224 2334 1895 2531 Fragments arranged by size: 2531 2334 1895 1224 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2097 2129 2185 2217 4387 Size: 32 135 32 525 32 56 32 2170 Cuts at: 4387 4419 4537 4556 4569 4588 5036 5068 6625 Size: 32 118 19 13 19 448 32 1557 Cuts at: 6625 6657 6699 6731 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2170 1557 525 448 135 118 56 42 32 32 32 32 32 32 32 32 19 19 13 HincII GTy'rAC Cuts at: 669 1657 1938 4575 5328 5574 669 Size: 988 281 2637 753 246 3079 Fragments arranged by size: 3079 2637 988 753 281 246 HindIII A'AGCT_T Cuts at: 748 3092 748 Size: 2344 5640 HpaI GTT'AAC Cuts at: 5574 5574 Size: 7984 KpnI G_GTAC'C Cuts at: 5309 5309 Size: 7984 MfeI C'AATT_G Cuts at: 5583 5583 Size: 7984 MluI A'CGCG_T Cuts at: 7860 7860 Size: 7984 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 7098 7282 1305 Size: 5793 184 2007 Fragments arranged by size: 5793 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6162 Fragments arranged by size: 6162 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2726 4394 5136 5942 6301 6460 518 Size: 648 1560 1668 742 806 359 159 2042 Fragments arranged by size: 2042 1668 1560 806 742 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2273 3796 3845 5400 6077 7225 7470 1606 Size: 667 1523 49 1555 677 1148 245 2120 Fragments arranged by size: 2120 1555 1523 1148 677 667 245 49 NcoI C'CATG_G Cuts at: 513 1065 2004 3513 513 Size: 552 939 1509 4984 Fragments arranged by size: 4984 1509 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7984 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7984 NotI GC'GGCC_GC Cuts at: 5431 5431 Size: 7984 NsiI A_TGCA'T Cuts at: 4767 4767 Size: 7984 NspI r_CATG'y Cuts at: 1132 1229 2672 4418 5135 5342 6887 1132 Size: 97 1443 1746 717 207 1545 2229 Fragments arranged by size: 2229 1746 1545 1443 717 207 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7984 PciI A'CATG_T Cuts at: 5131 6883 5131 Size: 1752 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7984 PmeI GTTT'AAAC Cuts at: 5288 5288 Size: 7984 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4983 5015 6053 6085 7566 7598 4983 Size: 32 1038 32 1481 32 5369 Fragments arranged by size: 5369 1481 1038 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2524 3548 3575 4019 2524 Size: 1024 27 444 6489 Fragments arranged by size: 6489 1024 444 27 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7984 PsiI TTA'TAA Cuts at: 4653 5554 4653 Size: 901 7083 PstI C_TGCA'G Cuts at: 830 3027 4687 4830 5336 830 Size: 2197 1660 143 506 3478 Fragments arranged by size: 3478 2197 1660 506 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7984 PvuII CAG'CTG Cuts at: 2273 3845 2273 Size: 1572 6412 SacI G_AGCT'C Cuts at: 2486 3145 4725 2486 Size: 659 1580 5745 Fragments arranged by size: 5745 1580 659 SalI G'TCGA_C Cuts at: 1936 5326 1936 Size: 3390 4594 SanDI GG'GwC_CC Cuts at: 3548 3548 Size: 7984 SbfI CC_TGCA'GG Cuts at: 5336 5336 Size: 7984 ScaI AGT'ACT Cuts at: 2473 6232 7944 2473 Size: 3759 1712 2513 Fragments arranged by size: 3759 2513 1712 SfcI C'TryA_G Cuts at: 826 1045 3023 4683 4826 5332 6467 7148 7339 Size: 219 1978 1660 143 506 1135 681 191 Cuts at: 7339 826 Size: 1471 Fragments arranged by size: 1978 1660 1471 1135 681 506 219 191 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7984 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7984 SmaI CCC'GGG Cuts at: 1872 5311 1872 Size: 3439 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3342 4460 4694 6088 6989 7251 Size: 197 926 1399 1118 234 1394 901 262 Cuts at: 7251 820 Size: 1553 Fragments arranged by size: 1553 1399 1394 1118 926 901 262 234 197 SnaBI TAC'GTA Cuts at: 493 5775 493 Size: 5282 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7984 SphI G_CATG'C Cuts at: 1132 5342 1132 Size: 4210 3774 SspI AAT'ATT Cuts at: 5 52 2980 3999 5908 5 Size: 47 2928 1019 1909 2081 Fragments arranged by size: 2928 2081 1909 1019 47 StuI AGG'CCT Cuts at: 1517 3048 1517 Size: 1531 6453 StyI C'CwwG_G Cuts at: 513 1065 2004 3177 3513 4015 4489 5423 513 Size: 552 939 1173 336 502 474 934 3074 Fragments arranged by size: 3074 1173 939 934 552 502 474 336 SwaI ATTT'AAAT Cuts at: 3975 3975 Size: 7984 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2054 3829 6011 6028 6181 6366 6811 Size: 1255 474 1775 2182 17 153 185 445 Cuts at: 6811 325 Size: 1498 Fragments arranged by size: 2182 1775 1498 1255 474 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2471 4755 6230 Size: 274 80 33 51 1443 494 2284 1475 Cuts at: 6230 7942 96 Size: 1712 138 Fragments arranged by size: 2284 1712 1475 1443 494 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4592 4624 4625 4657 7944 7976 4592 Size: 32 1 32 3287 32 4600 Fragments arranged by size: 4600 3287 32 32 32 1 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7984 XbaI T'CTAG_A Cuts at: 5320 5320 Size: 7984 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3046 3894 3046 Size: 848 7136 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7984 XmaI C'CCGG_G Cuts at: 1870 5309 1870 Size: 3439 4545 XmnI GAAnn'nnTTC Cuts at: 2288 2587 3387 3808 4389 4790 6113 2288 Size: 299 800 421 581 401 1323 4159 Fragments arranged by size: 4159 1323 800 581 421 401 299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5792 276 Size: 53 83 186 936 4258 2468 Fragments arranged by size: 4258 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI