Restriction map for
pFN21AA1825
(Linear) (Six-base) MAPSORT of: KIAA1825.seq Check: 8872 from: 1 to: 3612
KIAA1825.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 394 2462 2493 3612
Size: 394 2068 31 1119
Fragments arranged by size:
2068 1119 394 31
AccI GT'mk_AC
Cuts at: 0 908 1040 3209 3308 3612
Size: 908 132 2169 99 304
Fragments arranged by size:
2169 908 304 132 99
Acc65I G'GTAC_C
Cuts at: 0 1396 3612
Size: 1396 2216
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1228 1909 2575 2635 3612
Size: 1228 681 666 60 977
Fragments arranged by size:
1228 977 681 666 60
AfeI AGC'GCT
Cuts at: 0 1867 3612
Size: 1867 1745
AflIII A'CryG_T
Cuts at: 0 860 1535 3612
Size: 860 675 2077
Fragments arranged by size:
2077 860 675
AleI CACnn'nnGTG
Cuts at: 0 1165 1753 2941 3612
Size: 1165 588 1188 671
Fragments arranged by size:
1188 1165 671 588
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 46 295 1294 2389 2392 3612
Size: 46 249 999 1095 3 1220
Fragments arranged by size:
1220 1095 999 249 46 3
AlwNI CAG_nnn'CTG
Cuts at: 0 250 1851 2280 2478 2811 3063 3528 3612
Size: 250 1601 429 198 333 252 465 84
Fragments arranged by size:
1601 465 429 333 252 250 198 84
ApaI G_GGCC'C
Cuts at: 0 45 1710 1968 3612
Size: 45 1665 258 1644
Fragments arranged by size:
1665 1644 258 45
ApaLI G'TGCA_C
Cuts at: 0 364 1440 3190 3612
Size: 364 1076 1750 422
Fragments arranged by size:
1750 1076 422 364
ApoI r'AATT_y
Cuts at: 0 517 661 1856 3612
Size: 517 144 1195 1756
Fragments arranged by size:
1756 1195 517 144
AvaI C'yCGr_G
Cuts at: 0 82 348 2036 2102 2387 2687 2821 3122
Size: 82 266 1688 66 285 300 134 301
Cuts at: 3122 3241 3385 3612
Size: 119 144 227
Fragments arranged by size:
1688 301 300 285 266 227 144 134
119 82 66
BamHI G'GATC_C
Cuts at: 0 1559 3612
Size: 1559 2053
BanI G'GyrC_C
Cuts at: 0 30 188 238 432 690 770 999 1159
Size: 30 158 50 194 258 80 229 160
Cuts at: 1159 1396 1747 2593 2608 3612
Size: 237 351 846 15 1004
Fragments arranged by size:
1004 846 351 258 237 229 194 160
158 80 50 30 15
BanII G_rGCy'C
Cuts at: 0 45 464 767 1088 1553 1710 1930 1968
Size: 45 419 303 321 465 157 220 38
Cuts at: 1968 2049 2395 2759 3241 3612
Size: 81 346 364 482 371
Fragments arranged by size:
482 465 419 371 364 346 321 303
220 157 81 45 38
BbeI G_GCGC'C
Cuts at: 0 192 2612 3612
Size: 192 2420 1000
Fragments arranged by size:
2420 1000 192
BbsI GAAGACnn'nnnn_
Cuts at: 0 497 638 1100 3612
Size: 497 141 462 2512
Fragments arranged by size:
2512 497 462 141
BbvCI CC'TCA_GC
Cuts at: 0 1099 3612
Size: 1099 2513
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1013 1047 3612
Size: 1013 34 2565
Fragments arranged by size:
2565 1013 34
BclI T'GATC_A
Cuts at: 0 2234 2918 3612
Size: 2234 684 694
Fragments arranged by size:
2234 694 684
BglI GCCn_nnn'nGGC
Cuts at: 0 69 118 168 2435 2518 3479 3612
Size: 69 49 50 2267 83 961 133
Fragments arranged by size:
2267 961 133 83 69 50 49
BglII A'GATC_T
Cuts at: 0 2030 3612
Size: 2030 1582
Bme1580I G_kGCm'C
Cuts at: 0 35 45 368 1162 1444 1710 1887 1968
Size: 35 10 323 794 282 266 177 81
Cuts at: 1968 2002 3194 3612
Size: 34 1192 418
Fragments arranged by size:
1192 794 418 323 282 266 177 81
35 34 10
BmgBI CAC'GTC
Cuts at: 0 1011 1146 2604 2628 3612
Size: 1011 135 1458 24 984
Fragments arranged by size:
1458 1011 984 135 24
BmrI ACTGGGnnnn_n'
Cuts at: 0 1669 1989 2860 3612
Size: 1669 320 871 752
Fragments arranged by size:
1669 871 752 320
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2370 3612
Size: 2370 1242
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1108 1345 1456 2146 2434 2715 3040
Size: 571 537 237 111 690 288 281 325
Cuts at: 3040 3163 3612
Size: 123 449
Fragments arranged by size:
690 571 537 449 325 288 281 237
123 111
Bpu10I CC'TnA_GC
Cuts at: 0 1099 1472 2657 2704 2800 3199 3612
Size: 1099 373 1185 47 96 399 413
Fragments arranged by size:
1185 1099 413 399 373 96 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 2122 2158 3094 3612
Size: 520 1602 36 936 518
Fragments arranged by size:
1602 936 520 518 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 1321 2166 2810 3612
Size: 1321 845 644 802
Fragments arranged by size:
1321 845 802 644
BsaAI yAC'GTr
Cuts at: 0 2268 2571 2916 3612
Size: 2268 303 345 696
Fragments arranged by size:
2268 696 345 303
BsaHI Gr'CG_yC
Cuts at: 0 189 2609 3612
Size: 189 2420 1003
Fragments arranged by size:
2420 1003 189
BsaWI w'CCGG_w
Cuts at: 0 872 1249 1556 3612
Size: 872 377 307 2056
Fragments arranged by size:
2056 872 377 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 436 466 952 982 2036 2066 3612
Size: 436 30 486 30 1054 30 1546
Fragments arranged by size:
1546 1054 486 436 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 2785 3436 3444 3612
Size: 2785 651 8 168
Fragments arranged by size:
2785 651 168 8
BseYI C'CCAG_C
Cuts at: 0 257 263 406 1408 1923 2061 2076 2471
Size: 257 6 143 1002 515 138 15 395
Cuts at: 2471 2562 3612
Size: 91 1050
Fragments arranged by size:
1050 1002 515 395 257 143 138 91
15 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 372 457 862 1126 1192 1527 1963
Size: 301 71 85 405 264 66 335 436
Cuts at: 1963 2151 2266 2521 2919 3612
Size: 188 115 255 398 693
Fragments arranged by size:
693 436 405 398 335 301 264 255
188 115 85 71 66
BsiEI CG_ry'CG
Cuts at: 0 978 1463 1790 1955 2752 3564 3612
Size: 978 485 327 165 797 812 48
Fragments arranged by size:
978 812 797 485 327 165 48
BsiHKAI G_wGCw'C
Cuts at: 0 368 464 1444 3194 3612
Size: 368 96 980 1750 418
Fragments arranged by size:
1750 980 418 368 96
BsmI GAATG_Cn'
Cuts at: 0 2217 3612
Size: 2217 1395
BsmBI CGTCTCn'nnnn_
Cuts at: 0 972 1649 3612
Size: 972 677 1963
Fragments arranged by size:
1963 972 677
Bsp1286I G_dGCh'C
Cuts at: 0 35 45 368 464 767 1088 1162 1444
Size: 35 10 323 96 303 321 74 282
Cuts at: 1444 1553 1710 1887 1930 1968 2002 2049 2395
Size: 109 157 177 43 38 34 47 346
Cuts at: 2395 2759 3194 3241 3612
Size: 364 435 47 371
Fragments arranged by size:
435 371 364 346 323 321 303 282
177 157 109 96 74 47 47 43
38 35 34 10
BspEI T'CCGG_A
Cuts at: 0 872 1556 3612
Size: 872 684 2056
Fragments arranged by size:
2056 872 684
BspHI T'CATG_A
Cuts at: 0 2231 3612
Size: 2231 1381
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 394 2462 2493 3612
Size: 394 2068 31 1119
Fragments arranged by size:
2068 1119 394 31
BsrBI CCG'CTC
Cuts at: 0 980 2172 2253 2661 2673 3612
Size: 980 1192 81 408 12 939
Fragments arranged by size:
1192 980 939 408 81 12
BsrDI GCAATG_nn'
Cuts at: 0 372 486 932 3515 3612
Size: 372 114 446 2583 97
Fragments arranged by size:
2583 446 372 114 97
BsrFI r'CCGG_y
Cuts at: 0 117 167 1212 2336 3073 3612
Size: 117 50 1045 1124 737 539
Fragments arranged by size:
1124 1045 737 539 117 50
BsrGI T'GTAC_A
Cuts at: 0 3155 3269 3612
Size: 3155 114 343
Fragments arranged by size:
3155 343 114
BssHII G'CGCG_C
Cuts at: 0 96 98 377 3612
Size: 96 2 279 3235
Fragments arranged by size:
3235 279 96 2
BssSI C'ACGA_G
Cuts at: 0 304 463 1716 1753 3484 3612
Size: 304 159 1253 37 1731 128
Fragments arranged by size:
1731 1253 304 159 128 37
BstAPI GCAn_nnn'nTGC
Cuts at: 0 852 3612
Size: 852 2760
BstBI TT'CG_AA
Cuts at: 0 515 3612
Size: 515 3097
BstEII G'GTnAC_C
Cuts at: 0 1668 1759 2943 3612
Size: 1668 91 1184 669
Fragments arranged by size:
1668 1184 669 91
BstXI CCAn_nnnn'nTGG
Cuts at: 0 819 2604 3333 3612
Size: 819 1785 729 279
Fragments arranged by size:
1785 819 729 279
BstYI r'GATC_y
Cuts at: 0 284 641 869 1559 2030 2204 2966 3612
Size: 284 357 228 690 471 174 762 646
Fragments arranged by size:
762 690 646 471 357 284 228 174
BtgI C'CryG_G
Cuts at: 0 455 572 1199 1790 3320 3612
Size: 455 117 627 591 1530 292
Fragments arranged by size:
1530 627 591 455 292 117
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1350 1500 1932 2850 3612
Size: 1350 150 432 918 762
Fragments arranged by size:
1350 918 762 432 150
BtsI GCAGTG_nn'
Cuts at: 0 2255 2347 3612
Size: 2255 92 1265
Fragments arranged by size:
2255 1265 92
DraIII CAC_nnn'GTG
Cuts at: 0 2226 3612
Size: 2226 1386
DrdI GACnn_nn'nnGTC
Cuts at: 0 1677 3612
Size: 1677 1935
EaeI y'GGCC_r
Cuts at: 0 160 975 1496 1523 1643 1787 1952 2645
Size: 160 815 521 27 120 144 165 693
Cuts at: 2645 3071 3317 3323 3335 3428 3557 3612
Size: 426 246 6 12 93 129 55
Fragments arranged by size:
815 693 521 426 246 165 160 144
129 120 93 55 27 12 6
EagI C'GGCC_G
Cuts at: 0 975 1787 1952 3612
Size: 975 812 165 1660
Fragments arranged by size:
1660 975 812 165
EarI CTCTTCn'nnn_
Cuts at: 0 1297 1351 3091 3612
Size: 1297 54 1740 521
Fragments arranged by size:
1740 1297 521 54
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2473 3612
Size: 2473 1139
EcoICRI GAG'CTC
Cuts at: 0 462 3612
Size: 462 3150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1108 1228 1345 1456 1909 2146 2434
Size: 571 537 120 117 111 453 237 288
Cuts at: 2434 2575 2635 2715 3040 3163 3612
Size: 141 60 80 325 123 449
Fragments arranged by size:
571 537 453 449 325 288 237 141
123 120 117 111 80 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2787 2803 3612
Size: 2787 16 809
Fragments arranged by size:
2787 809 16
EcoO109I rG'GnC_Cy
Cuts at: 0 41 1404 1707 1964 1965 2121 3525 3587
Size: 41 1363 303 257 1 156 1404 62
Cuts at: 3587 3612
Size: 25
Fragments arranged by size:
1404 1363 303 257 156 62 41 25
1
EcoRI G'AATT_C
Cuts at: 0 517 3612
Size: 517 3095
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 514 3612
Size: 514 3098
FspI TGC'GCA
Cuts at: 0 1282 3612
Size: 1282 2330
HaeII r_GCGC'y
Cuts at: 0 124 181 192 1869 2062 2355 2612 2641
Size: 124 57 11 1677 193 293 257 29
Cuts at: 2641 2977 3553 3612
Size: 336 576 59
Fragments arranged by size:
1677 576 336 293 257 193 124 59
57 29 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 493 525 952 984 2036 2068 3612
Size: 493 32 427 32 1052 32 1544
Fragments arranged by size:
1544 1052 493 427 32 32 32
HincII GTy'rAC
Cuts at: 0 1218 1503 1613 3297 3612
Size: 1218 285 110 1684 315
Fragments arranged by size:
1684 1218 315 285 110
KasI G'GCGC_C
Cuts at: 0 188 2608 3612
Size: 188 2420 1004
Fragments arranged by size:
2420 1004 188
KpnI G_GTAC'C
Cuts at: 0 1400 3612
Size: 1400 2212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 3612
Size: 845 2767
MscI TGG'CCA
Cuts at: 0 1525 2647 3319 3325 3337 3430 3612
Size: 1525 1122 672 6 12 93 182
Fragments arranged by size:
1525 1122 672 182 93 12 6
MslI CAynn'nnrTG
Cuts at: 0 479 817 1165 1753 2941 3172 3319 3325
Size: 479 338 348 588 1188 231 147 6
Cuts at: 3325 3331 3496 3612
Size: 6 165 116
Fragments arranged by size:
1188 588 479 348 338 231 165 147
116 6 6
MspA1I CmG'CkG
Cuts at: 0 10 250 652 1031 1223 1375 1737 2478
Size: 10 240 402 379 192 152 362 741
Cuts at: 2478 2748 2933 3612
Size: 270 185 679
Fragments arranged by size:
741 679 402 379 362 270 240 192
185 152 10
NaeI GCC'GGC
Cuts at: 0 119 2338 3075 3612
Size: 119 2219 737 537
Fragments arranged by size:
2219 737 537 119
NarI GG'CG_CC
Cuts at: 0 189 2609 3612
Size: 189 2420 1003
Fragments arranged by size:
2420 1003 189
NcoI C'CATG_G
Cuts at: 0 3320 3612
Size: 3320 292
NgoMIV G'CCGG_C
Cuts at: 0 117 2336 3073 3612
Size: 117 2219 737 539
Fragments arranged by size:
2219 737 539 117
NspI r_CATG'y
Cuts at: 0 864 1539 2264 2623 3612
Size: 864 675 725 359 989
Fragments arranged by size:
989 864 725 675 359
PasI CC'CwG_GG
Cuts at: 0 1266 3612
Size: 1266 2346
PciI A'CATG_T
Cuts at: 0 860 1535 3612
Size: 860 675 2077
Fragments arranged by size:
2077 860 675
PflMI CCAn_nnn'nTGG
Cuts at: 0 2226 3612
Size: 2226 1386
PfoI T'CCnGG_A
Cuts at: 0 626 2198 2731 3533 3612
Size: 626 1572 533 802 79
Fragments arranged by size:
1572 802 626 533 79
PmlI CAC'GTG
Cuts at: 0 2268 2916 3612
Size: 2268 648 696
Fragments arranged by size:
2268 696 648
PpuMI rG'GwC_Cy
Cuts at: 0 1404 3525 3587 3612
Size: 1404 2121 62 25
Fragments arranged by size:
2121 1404 62 25
PspOMI G'GGCC_C
Cuts at: 0 41 1706 1964 3612
Size: 41 1665 258 1648
Fragments arranged by size:
1665 1648 258 41
PspXI vC'TCGA_Gb
Cuts at: 0 2821 3612
Size: 2821 791
PstI C_TGCA'G
Cuts at: 0 1374 2318 2459 3575 3612
Size: 1374 944 141 1116 37
Fragments arranged by size:
1374 1116 944 141 37
PvuII CAG'CTG
Cuts at: 0 652 1375 1737 2478 3612
Size: 652 723 362 741 1134
Fragments arranged by size:
1134 741 723 652 362
RsrII CG'GwC_CG
Cuts at: 0 1247 3612
Size: 1247 2365
SacI G_AGCT'C
Cuts at: 0 464 3612
Size: 464 3148
SanDI GG'GwC_CC
Cuts at: 0 3587 3612
Size: 3587 25
SbfI CC_TGCA'GG
Cuts at: 0 2459 3612
Size: 2459 1153
ScaI AGT'ACT
Cuts at: 0 784 3612
Size: 784 2828
SexAI A'CCwGG_T
Cuts at: 0 995 3612
Size: 995 2617
SfcI C'TryA_G
Cuts at: 0 792 1370 1691 2314 2455 2953 2997 3055
Size: 792 578 321 623 141 498 44 58
Cuts at: 3055 3571 3612
Size: 516 41
Fragments arranged by size:
792 623 578 516 498 321 141 58
44 41
SfoI GGC'GCC
Cuts at: 0 190 2610 3612
Size: 190 2420 1002
Fragments arranged by size:
2420 1002 190
SmaI CCC'GGG
Cuts at: 0 84 2038 2104 2389 2689 3612
Size: 84 1954 66 285 300 923
Fragments arranged by size:
1954 923 300 285 84 66
SmlI C'TyrA_G
Cuts at: 0 499 2137 2173 2821 3109 3612
Size: 499 1638 36 648 288 503
Fragments arranged by size:
1638 648 503 499 288 36
SphI G_CATG'C
Cuts at: 0 2264 2623 3612
Size: 2264 359 989
Fragments arranged by size:
2264 989 359
SrfI GCCC'GGGC
Cuts at: 0 84 3612
Size: 84 3528
StuI AGG'CCT
Cuts at: 0 832 3612
Size: 832 2780
StyI C'CwwG_G
Cuts at: 0 1163 1400 2186 3320 3612
Size: 1163 237 786 1134 292
Fragments arranged by size:
1163 1134 786 292 237
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 226 267 1449 1747 2620 3612
Size: 226 41 1182 298 873 992
Fragments arranged by size:
1182 992 873 298 226 41
TatI w'GTAC_w
Cuts at: 0 782 1538 3155 3269 3612
Size: 782 756 1617 114 343
Fragments arranged by size:
1617 782 756 343 114
Tth111I GACn'n_nGTC
Cuts at: 0 964 3565 3612
Size: 964 2601 47
Fragments arranged by size:
2601 964 47
XbaI T'CTAG_A
Cuts at: 0 1767 3612
Size: 1767 1845
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 462 1623 2847 3612
Size: 462 1161 1224 765
Fragments arranged by size:
1224 1161 765 462
XhoI C'TCGA_G
Cuts at: 0 2821 3612
Size: 2821 791
XmaI C'CCGG_G
Cuts at: 0 82 2036 2102 2387 2687 3612
Size: 82 1954 66 285 300 925
Fragments arranged by size:
1954 925 300 285 82 66
XmnI GAAnn'nnTTC
Cuts at: 0 1822 3612
Size: 1822 1790
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI
PvuII RsrII SacI SanDI SbfI ScaI SexAI SfcI
SfoI SmaI SmlI SphI SrfI StuI StyI TaqII
TatI Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AhdI AloI AscI AseI
AsiSI AvrII BaeI BciVI BfrBI BlpI BmtI BsaBI
BsiWI BstZ17I Bsu36I ClaI CspCI DraI EcoRV FseI
FspAI HindIII HpaI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PmeI PpiI PshAI PsiI PsrI
PvuI SacII SalI SapI SfiI SgfI SgrAI SnaBI
SpeI SspI SwaI TstI ZraI