Restriction map for pFN21AA1531
 (Circular) (Six-base) MAPSORT of: pFN21AA1531.seq  Check: 540  from: 1  to: 8068



pFN21AA1531  8068 bp

 With 182 enzymes: SgfI * 

                        January 16, 2009 15:23  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   2604    3513    2604 
   Size:        909    7159
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    5295    5878     278 
   Size:         53      83     186     936    3759     583    2468

  Fragments arranged by size: 

                3759    2468     936     583     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    3435    3672    5411    1937 
   Size:       1498     237    1739    4594

  Fragments arranged by size: 

                4594    1739    1498     237
 
Acc65I G'GTAC_C

Cuts at:   2847    5389    2847 
   Size:       2542    5526
 
AclI AA'CG_TT

Cuts at:   1563    6195    6568    1563 
   Size:       4632     373    3063

  Fragments arranged by size: 

                4632    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2426    3082    6131    7515    7783    1632 
   Size:        794     656    3049    1384     268    1917

  Fragments arranged by size: 

                3049    1917    1384     794     656     268
 
AfeI AGC'GCT

Cuts at:   4481    4481 
   Size:       8068
 
AflII C'TTAA_G

Cuts at:    820    1017    2549     820 
   Size:        197    1532    6339

  Fragments arranged by size: 

                6339    1532     197
 
AflIII A'CryG_T

Cuts at:   1203    6967    7944    1203 
   Size:       5764     977    1327

  Fragments arranged by size: 

                5764    1327     977
 
AgeI A'CCGG_T

Cuts at:   6899    6899 
   Size:       8068
 
AhdI GACnn_n'nnGTC

Cuts at:   6797    6797 
   Size:       8068
 
AleI CACnn'nnGTG

Cuts at:   1166    3164    3278    3293    4560    1166 
   Size:       1998     114      15    1267    4674

  Fragments arranged by size: 

                4674    1998    1267     114      15
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3503    3704    3893    1184 
   Size:       2319     201     189    5359

  Fragments arranged by size: 

                5359    2319     201     189
 
AlwNI CAG_nnn'CTG

Cuts at:   2689    5811    7383    2689 
   Size:       3122    1572    3374

  Fragments arranged by size: 

                3374    3122    1572
 
ApaI G_GGCC'C

Cuts at:   2620    2731    4368    4648    4695    4751    2620 
   Size:        111    1637     280      47      56    5937

  Fragments arranged by size: 

                5937    1637     280     111      56      47
 
ApaLI G'TGCA_C

Cuts at:   2986    4880    6125    7281    2986 
   Size:       1894    1245    1156    3773

  Fragments arranged by size: 

                3773    1894    1245    1156
 
ApoI r'AATT_y

Cuts at:   1493    3419    5377    5603    5755    7864    1493 
   Size:       1926    1958     226     152    2109    1697

  Fragments arranged by size: 

                2109    1958    1926    1697     226     152
 
AscI GG'CGCG_CC

Cuts at:   2103    4594    2103 
   Size:       2491    5577
 
AseI AT'TA_AT

Cuts at:    160    1032    6622     160 
   Size:        872    5590    1606

  Fragments arranged by size: 

                5590    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       8068
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2090    2097    2143    2234    3187    3195    4361 
   Size:         73     147       7      46      91     953       8    1166

Cuts at:   4361    4491    4641    4845    5065    5393    1870 
   Size:        130     150     204     220     328    4545

  Fragments arranged by size: 

                4545    1166     953     328     220     204     150     147
                 130      91      73      46       8       7
 
BamHI G'GATC_C

Cuts at:   5398    5765    5398 
   Size:        367    7701
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2009    2035    2086    2258    2381 
   Size:        325     217     627     222      26      51     172     123

Cuts at:   2381    2567    2847    3182    3191    3554    3644    3955    4130 
   Size:        186     280     335       9     363      90     311     175

Cuts at:   4130    4841    5069    5264    5389    6844     618 
   Size:        711     228     195     125    1455    1842

  Fragments arranged by size: 

                1842    1455     711     627     363     335     325     311
                 280     228     222     217     195     186     175     172
                 125     123      90      51      26       9
 
BanII G_rGCy'C

Cuts at:   1391    2241    2301    2620    2731    2863    3945    4368    4465 
   Size:        850      60     319     111     132    1082     423      97

Cuts at:   4465    4648    4695    4751    5097    5387    1391 
   Size:        183      47      56     346     290    4072

  Fragments arranged by size: 

                4072    1082     850     423     346     319     290     183
                 132     111      97      60      56      47
 
BbeI G_GCGC'C

Cuts at:   2013    2039    2090    2385    3648    4134    5073    5268    2013 
   Size:         26      51     295    1263     486     939     195    4813

  Fragments arranged by size: 

                4813    1263     939     486     295     195      51      26
            
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    4540    5342     928 
   Size:        971    2641     802    3654

  Fragments arranged by size: 

                3654    2641     971     802
 
BbvCI CC'TCA_GC

Cuts at:   3584    4271    3584 
   Size:        687    7381
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3059    3093    3529    3563    6259    6293    1903 
   Size:         34    1122      34     436      34    2696      34    3678

  Fragments arranged by size: 

                3678    2696    1122     436      34      34      34      34
            
 
BciVI GTATCCnnnnn_n'

Cuts at:   3162    5960    7170    3162 
   Size:       2798    1210    4060

  Fragments arranged by size: 

                4060    2798    1210
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       8068
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    2161    4451    4460 
   Size:        107     122      71     631     605     489    2290       9

Cuts at:   4460    5160    5262    6679     136 
   Size:        700     102    1417    1525

  Fragments arranged by size: 

                2290    1525    1417     700     631     605     489     122
                 107     102      71       9
 
BglII A'GATC_T

Cuts at:   2416    8063    2416 
   Size:       5647    2421
 
BlpI GC'TnA_GC

Cuts at:   2165    3617    5485    2165 
   Size:       1452    1868    4748

  Fragments arranged by size: 

                4748    1868    1452
 
Bme1580I G_kGCm'C

Cuts at:   1790    2570    2620    2674    2731    2990    3171    3187    3194 
   Size:        780      50      54      57     259     181      16       7

Cuts at:   3194    3559    3958    4368    4648    4695    4751    4844    4884 
   Size:        365     399     410     280      47      56      93      40

Cuts at:   4884    6129    7285    1790 
   Size:       1245    1156    2573

  Fragments arranged by size: 

                2573    1245    1156     780     410     399     365     280
                 259     181      93      57      56      54      50      47
                  40      16       7
 
BmgBI CAC'GTC

Cuts at:   1318    4579    1318 
   Size:       3261    4807
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2661    3990    4347 
   Size:        416      53      83     399      24    1244    1329     357

Cuts at:   4347    5799    6757     442 
   Size:       1452     958    1753

  Fragments arranged by size: 

                1753    1452    1329    1244     958     416     399     357
                  83      53      24
 
BmtI G_CTAG'C

Cuts at:   1056    3372    1056 
   Size:       2316    5752
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2354    2494    2684    2753    3062    3581    3970    4047 
   Size:       1115     140     190      69     309     519     389      77

Cuts at:   4047    4274    5009    5231    6728    1239 
   Size:        227     735     222    1497    2579

  Fragments arranged by size: 

                2579    1497    1115     735     519     389     309     227
                 222     190     140      77      69
 
Bpu10I CC'TnA_GC

Cuts at:   2682    3584    4271    4289    2682 
   Size:        902     687      18    6461

  Fragments arranged by size: 

                6461     902     687      18
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2296    4688    4940    5177    6193    7058    7356    2296 
   Size:       2392     252     237    1016     865     298    3008

  Fragments arranged by size: 

                3008    2392    1016     865     298     252     237
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2447    3038    6731     882 
   Size:        617     948     591    3693    2219

  Fragments arranged by size: 

                3693    2219     948     617     591
 
BsaAI yAC'GTr

Cuts at:    493    1204    5859     493 
   Size:        711    4655    2702

  Fragments arranged by size: 

                4655    2702     711
 
BsaBI GATnn'nnATC

Cuts at:   3305    8062    3305 
   Size:       4757    3311
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2010    2036    2087    2175 
   Size:         53      83     186     936     477      26      51      88

Cuts at:   2175    2382    3645    4117    4131    4358    4891    5070    5265 
   Size:        207    1263     472      14     227     533     179     195

Cuts at:   5265    5292    5875    6257    6892     275 
   Size:         27     583     382     635    1451

  Fragments arranged by size: 

                1451    1263     936     635     583     533     477     472
                 382     227     207     195     186     179      88      83
                  53      51      27      26      14
 
BsaWI w'CCGG_w

Cuts at:   2430    4008    5339    6501    6899    7173    7320    7731    2430 
   Size:       1578    1331    1162     398     274     147     411    2767

  Fragments arranged by size: 

                2767    1578    1331    1162     411     398     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2492    2522    3725    3755    3872 
   Size:         30     165      30    1089      30    1203      30     117

Cuts at:   3872    3902    4982    5012    1178 
   Size:         30    1080      30    4234

  Fragments arranged by size: 

                4234    1203    1089    1080     165     117      30      30
                  30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3877    4280    1186 
   Size:       2691     403    4974

  Fragments arranged by size: 

                4974    2691     403
 
BseYI C'CCAG_C

Cuts at:   2709    3228    3519    3597    4476    7271    7767    2709 
   Size:        519     291      78     879    2795     496    3010

  Fragments arranged by size: 

                3010    2795     879     519     496     291      78
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2227    2393    2723    2905    3644    3664    3863 
   Size:        573     491     166     330     182     739      20     199

Cuts at:   3863    4522    5003    1163 
   Size:        659     481    4228

  Fragments arranged by size: 

                4228     739     659     573     491     481     330     199
                 182     166      20
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3226    4812    5518    6279    7307    1540 
   Size:        275     186    1225    1586     706     761    1028    2301

  Fragments arranged by size: 

                2301    1586    1225    1028     761     706     275     186
            
 
BsiHKAI G_wGCw'C

Cuts at:   2144    2810    2990    3504    3933    3945    4884    6129    6214 
   Size:        666     180     514     429      12     939    1245      85

Cuts at:   6214    7285    2144 
   Size:       1071    2927

  Fragments arranged by size: 

                2927    1245    1071     939     666     514     429     180
                  85      12
 
BsmI GAATG_Cn'

Cuts at:   5579    5672    5579 
   Size:         93    7975
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2144    2241    2301    2570    2620    2674    2731 
   Size:        399     354      97      60     269      50      54      57

Cuts at:   2731    2810    2863    2990    3171    3187    3194    3504    3559 
   Size:         79      53     127     181      16       7     310      55

Cuts at:   3559    3933    3945    3958    4368    4465    4648    4695    4751 
   Size:        374      12      13     410      97     183      47      56

Cuts at:   4751    4844    4884    5097    5387    6129    6214    7285    1391 
   Size:         93      40     213     290     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     410     399     374     354     310
                 290     269     213     183     181     127      97      97
                  93      85      79      60      57      56      55      54
                  53      50      47      40      16      13      12       7
            
 
BspEI T'CCGG_A

Cuts at:   7731    7731 
   Size:       8068
 
BspHI T'CATG_A

Cuts at:   5957    7687    5957 
   Size:       1730    6338
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2604    3513    4776    5423     844 
   Size:       1760     909    1263     647    3489

  Fragments arranged by size: 

                3489    1760    1263     909     647
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2152    2169    3983    5010    5232    5242    5955 
   Size:        429     329      17    1814    1027     222      10     713

Cuts at:   5955    1394 
   Size:       3507

  Fragments arranged by size: 

                3507    1814    1027     713     429     329     222      17
                  10
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2311    6563    6737      65 
   Size:       1183    1063    4252     174    1396

  Fragments arranged by size: 

                4252    1396    1183    1063     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3207    3221    4811    4834    4950    5072    6712 
   Size:        110    1397      14    1590      23     116     122    1640

Cuts at:   6712    6899    1700 
   Size:        187    2869

  Fragments arranged by size: 

                2869    1640    1590    1397     187     122     116     110
                  23      14
 
BsrGI T'GTAC_A

Cuts at:     96    3285    3351      96 
   Size:       3189      66    4813

  Fragments arranged by size: 

                4813    3189      66
 
BssHII G'CGCG_C

Cuts at:   1925    2042    2103    4344    4594    5205    1925 
   Size:        117      61    2241     250     611    4788

  Fragments arranged by size: 

                4788    2241     611     250     117      61
 
BssSI C'ACGA_G

Cuts at:   4588    6128    7140    4588 
   Size:       1540    1012    5516

  Fragments arranged by size: 

                5516    1540    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    3775     794 
   Size:       2981    5087
 
BstBI TT'CG_AA

Cuts at:   6872    6872 
   Size:       8068
 
BstEII G'GTnAC_C

Cuts at:   1184    4420    1184 
   Size:       3236    4832
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2821    3637    2821 
   Size:        816    7252
 
BstYI r'GATC_y

Cuts at:   1972    2416    4173    5398    5765    6150    6167    7619    7705 
   Size:        444    1757    1225     367     385      17    1452      86

Cuts at:   7705    7717    8063    1972 
   Size:         12     346    1977

  Fragments arranged by size: 

                1977    1757    1452    1225     444     385     367     346
                  86      17      12
 
BstZ17I GTA'TAC

Cuts at:   3673    3673 
   Size:       8068
 
Bsu36I CC'TnA_GG

Cuts at:   2348    2971    2348 
   Size:        623    7445
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2066    2997    3088    3666    4620    4885 
   Size:        552     939      62     931      91     578     954     265

Cuts at:   4885     513 
   Size:       3696

  Fragments arranged by size: 

                3696     954     939     931     578     552     265      91
                  62
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    5210     487 
   Size:        683      56    3984    3345

  Fragments arranged by size: 

                3984    3345     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2327    4476    5584    6378    6398    2327 
   Size:       2149    1108     794      20    3997

  Fragments arranged by size: 

                3997    2149    1108     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       8068
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    8033
 
DraI TTT'AAA

Cuts at:   5372    5372 
   Size:       8068
 
DraIII CAC_nnn'GTG

Cuts at:   3667    3667 
   Size:       8068
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    7075     809 
   Size:        507    5759    1802

  Fragments arranged by size: 

                5759    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2898    3223    3474 
   Size:         54    1425      50     275      18    1068     325     251

Cuts at:   3474    4443    4729    4809    5515    6404    8033       8 
   Size:        969     286      80     706     889    1629      43

  Fragments arranged by size: 

                1629    1425    1068     969     889     706     325     286
                 275     251      80      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    3223    4809    5515    1537 
   Size:        275    1411    1586     706    4090

  Fragments arranged by size: 

                4090    1586    1411     706     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3710    4547    5345    5998    1353 
   Size:       2357     837     798     653    3423

  Fragments arranged by size: 

                3423    2357     837     798     653
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2209    4519    4585    6655    7029    7175 
   Size:       1064     138     620    2310      66    2070     374     146

Cuts at:   7175     387 
   Size:       1280

  Fragments arranged by size: 

                2310    2070    1280    1064     620     374     146     138
                  66
 
EcoICRI GAG'CTC

Cuts at:   3943    3943 
   Size:       8068
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2354    2426    2494    2684    2753    3062    3082 
   Size:        393     722      72      68     190      69     309      20

Cuts at:   3082    3581    3970    4047    4274    5009    5231    6131    6728 
   Size:        499     389      77     227     735     222     900     597

Cuts at:   6728    7515    7783    1239 
   Size:        787     268    1524

  Fragments arranged by size: 

                1524     900     787     735     722     597     499     393
                 389     309     268     227     222     190      77      72
                  69      68      20
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2702    3628    2702 
   Size:        926    7142
 
EcoO109I rG'GnC_Cy

Cuts at:   2218    2464    2686    2727    2728    3976    4208    4245    4365 
   Size:        246     222      41       1    1248     232      37     120

Cuts at:   4365    4645    4691    4692    4748    4960    2218 
   Size:        280      46       1      56     212    5326

  Fragments arranged by size: 

                5326    1248     280     246     232     222     212     120
                  56      46      41      37       1       1
 
EcoRI G'AATT_C

Cuts at:   5377    5755    5377 
   Size:        378    7690
 
FspI TGC'GCA

Cuts at:   6574    6574 
   Size:       8068
 
HaeII r_GCGC'y

Cuts at:   1678    2013    2039    2090    2385    3624    3648    4134    4418 
   Size:        335      26      51     295    1239      24     486     284

Cuts at:   4418    4483    4988    5073    5133    5170    5178    5268    7215 
   Size:         65     505      85      60      37       8      90    1947

Cuts at:   7215    1678 
   Size:       2531

  Fragments arranged by size: 

                2531    1947    1239     505     486     335     295     284
                  90      85      65      60      51      37      26      24
                   8
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2425    2457    2935    2967    3319 
   Size:         32     135      32     853      32     478      32     352

Cuts at:   3319    3351    4982    4999    5014    5031    6709    6741    6783 
   Size:         32    1631      17      15      17    1678      32      42

Cuts at:   6783    6815    1373 
   Size:         32    2626

  Fragments arranged by size: 

                2626    1678    1631     853     478     352     135      42
                  32      32      32      32      32      32      32      17
                  17      15
 
HincII GTy'rAC

Cuts at:    669    1657    1938    3436    4069    5412    5658     669 
   Size:        988     281    1498     633    1343     246    3079

  Fragments arranged by size: 

                3079    1498    1343     988     633     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       8068
 
HpaI GTT'AAC

Cuts at:   5658    5658 
   Size:       8068
 
KasI G'GCGC_C

Cuts at:   2009    2035    2086    2381    3644    4130    5069    5264    2009 
   Size:         26      51     295    1263     486     939     195    4813

  Fragments arranged by size: 

                4813    1263     939     486     295     195      51      26
            
 
KpnI G_GTAC'C

Cuts at:   2851    5393    2851 
   Size:       2542    5526
 
MfeI C'AATT_G

Cuts at:   5667    5667 
   Size:       8068
 
MluI A'CGCG_T

Cuts at:   7944    7944 
   Size:       8068
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    2448    2506    2622    7182    7366    1305 
   Size:       1143      58     116    4560     184    2007

  Fragments arranged by size: 

                4560    2007    1143     184     116      58
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    3476    4445      10 
   Size:         54    1425     343    1644     969    3633

  Fragments arranged by size: 

                3633    1644    1425     969     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2960    3164    3278    3293    3608    3749    3800 
   Size:        648    1794     204     114      15     315     141      51

Cuts at:   3800    4145    4560    5300    6026    6385    6544     518 
   Size:        345     415     740     726     359     159    2042

  Fragments arranged by size: 

                2042    1794     740     726     648     415     359     345
                 315     204     159     141     114      51      15
 
MspA1I CmG'CkG

Cuts at:   1606    2059    2133    2211    2638    2844    3090    3228    3343 
   Size:        453      74      78     427     206     246     138     115

Cuts at:   3343    4061    4299    4506    4622    4728    4738    4774    5083 
   Size:        718     238     207     116     106      10      36     309

Cuts at:   5083    5322    5484    6161    7309    7554    1606 
   Size:        239     162     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     718     677     453     427     309     246
                 245     239     238     207     206     162     138     116
                 115     106      78      74      36      10
 
NaeI GCC'GGC

Cuts at:   3223    4813    4836    5074    3223 
   Size:       1590      23     238    6217

  Fragments arranged by size: 

                6217    1590     238      23
 
NarI GG'CG_CC

Cuts at:   2010    2036    2087    2382    3645    4131    5070    5265    2010 
   Size:         26      51     295    1263     486     939     195    4813

  Fragments arranged by size: 

                4813    1263     939     486     295     195      51      26
            
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2997     513 
   Size:        552     939     993    5584

  Fragments arranged by size: 

                5584     993     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       8068
 
NgoMIV G'CCGG_C

Cuts at:   3221    4811    4834    5072    3221 
   Size:       1590      23     238    6217

  Fragments arranged by size: 

                6217    1590     238      23
 
NheI G'CTAG_C

Cuts at:   1052    3368    1052 
   Size:       2316    5752
 
NotI GC'GGCC_GC

Cuts at:   5515    5515 
   Size:       8068
 
NspI r_CATG'y

Cuts at:   1132    1229    2681    3775    5426    6971    1132 
   Size:         97    1452    1094    1651    1545    2229

  Fragments arranged by size: 

                2229    1651    1545    1452    1094      97
 
PasI CC'CwG_GG

Cuts at:   1348    2613    2689    3172    3630    4534    1348 
   Size:       1265      76     483     458     904    4882

  Fragments arranged by size: 

                4882    1265     904     483     458      76
 
PciI A'CATG_T

Cuts at:   6967    6967 
   Size:       8068
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3865    4127    4738    1108 
   Size:       2757     262     611    4438

  Fragments arranged by size: 

                4438    2757     611     262
 
PfoI T'CCnGG_A

Cuts at:   2223    2700    3755    4396    2223 
   Size:        477    1055     641    5895

  Fragments arranged by size: 

                5895    1055     641     477
 
PmeI GTTT'AAAC

Cuts at:   5372    5372 
   Size:       8068
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2637    2669    6137    6169    7650    7682    2637 
   Size:         32    3468      32    1481      32    3023

  Fragments arranged by size: 

                3468    3023    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2218    3976    4208    2218 
   Size:       1758     232    6078

  Fragments arranged by size: 

                6078    1758     232
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       8068
 
PsiI TTA'TAA

Cuts at:   5638    5638 
   Size:       8068
 
PspOMI G'GGCC_C

Cuts at:   2616    2727    4364    4644    4691    4747    2616 
   Size:        111    1637     280      47      56    5937

  Fragments arranged by size: 

                5937    1637     280     111      56      47
 
PstI C_TGCA'G

Cuts at:    830    2637    3150    3542    4066    4773    5082    5420     830 
   Size:       1807     513     392     524     707     309     338    3478

  Fragments arranged by size: 

                3478    1807     707     524     513     392     338     309
            
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       8068
 
PvuII CAG'CTG

Cuts at:   2133    2638    3343    4061    4506    4774    5083    2133 
   Size:        505     705     718     445     268     309    5118

  Fragments arranged by size: 

                5118     718     705     505     445     309     268
 
SacI G_AGCT'C

Cuts at:   3945    3945 
   Size:       8068
 
SacII CC_GC'GG

Cuts at:   3091    4623    3091 
   Size:       1532    6536
 
SalI G'TCGA_C

Cuts at:   1936    3434    5410    1936 
   Size:       1498    1976    4594

  Fragments arranged by size: 

                4594    1976    1498
 
SanDI GG'GwC_CC

Cuts at:   3976    3976 
   Size:       8068
 
SapI GCTCTTCn'nnn_

Cuts at:   5345    5345 
   Size:       8068
 
SbfI CC_TGCA'GG

Cuts at:   5420    5420 
   Size:       8068
 
ScaI AGT'ACT

Cuts at:   6316    8028    6316 
   Size:       1712    6356
 
SfcI C'TryA_G

Cuts at:    826    1045    2633    3146    3538    4062    4769    5078    5416 
   Size:        219    1588     513     392     524     707     309     338

Cuts at:   5416    6551    7232    7423     826 
   Size:       1135     681     191    1471

  Fragments arranged by size: 

                1588    1471    1135     707     681     524     513     392
                 338     309     219     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   4451    5160    4451 
   Size:        709    7359
 
SfoI GGC'GCC

Cuts at:   2011    2037    2088    2383    3646    4132    5071    5266    2011 
   Size:         26      51     295    1263     486     939     195    4813

  Fragments arranged by size: 

                4813    1263     939     486     295     195      51      26
            
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       8068
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    3207    1700 
   Size:       1507    6561
 
SmaI CCC'GGG

Cuts at:   1872    3189    4363    4493    4643    4847    5067    5395    1872 
   Size:       1317    1174     130     150     204     220     328    4545

  Fragments arranged by size: 

                4545    1317    1174     328     220     204     150     130
            
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2275    2549    4703    4955    5192    6172 
   Size:        197     926     332     274    2154     252     237     980

Cuts at:   6172    7073    7335     820 
   Size:        901     262    1553

  Fragments arranged by size: 

                2154    1553     980     926     901     332     274     262
                 252     237     197
 
SnaBI TAC'GTA

Cuts at:    493    5859     493 
   Size:       5366    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       8068
 
SphI G_CATG'C

Cuts at:   1132    2681    5426    1132 
   Size:       1549    2745    3774

  Fragments arranged by size: 

                3774    2745    1549
 
SrfI GCCC'GGGC

Cuts at:   4363    5067    4363 
   Size:        704    7364
 
SspI AAT'ATT

Cuts at:      5      52    5992       5 
   Size:         47    5940    2081

  Fragments arranged by size: 

                5940    2081      47
 
StuI AGG'CCT

Cuts at:   1517    3536    4937    1517 
   Size:       2019    1401    4648

  Fragments arranged by size: 

                4648    2019    1401
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2157    2997    3004    4126    4227    4914 
   Size:        552     939     153     840       7    1122     101     687

Cuts at:   4914    5271    5507     513 
   Size:        357     236    3074

  Fragments arranged by size: 

                3074    1122     939     840     687     552     357     236
                 153     101       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    3542    6095    6112    6265    6450    6895     325 
   Size:       1255    1962    2553      17     153     185     445    1498

  Fragments arranged by size: 

                2553    1962    1498    1255     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3285    3351    4437 
   Size:        274      80      33      51    1443    1308      66    1086

Cuts at:   4437    6314    8026      96 
   Size:       1877    1712     138

  Fragments arranged by size: 

                1877    1712    1443    1308    1086     274     138      80
                  66      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3086    3118    3872    3904    8028    8060    3086 
   Size:         32     754      32    4124      32    3094

  Fragments arranged by size: 

                4124    3094     754      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2947    5291    1316 
   Size:       1631    2344    4093

  Fragments arranged by size: 

                4093    2344    1631
 
XbaI T'CTAG_A

Cuts at:   5404    5404 
   Size:       8068
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3022    3847    4771    3022 
   Size:        825     924    6319

  Fragments arranged by size: 

                6319     924     825
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       8068
 
XmaI C'CCGG_G

Cuts at:   1870    3187    4361    4491    4641    4845    5065    5393    1870 
   Size:       1317    1174     130     150     204     220     328    4545

  Fragments arranged by size: 

                4545    1317    1174     328     220     204     150     130
            
 
XmnI GAAnn'nnTTC

Cuts at:   6197    6197 
   Size:       8068
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    5293    5876     276 
   Size:         53      83     186     936    3759     583    2468

  Fragments arranged by size: 

                3759    2468     936     583     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FspI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NspI     PasI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI     PstI     PvuI
    PvuII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AloI    AvrII     BaeI    BfrBI     BplI    BsiWI    BsmBI    EcoRV
     FalI     FseI    FspAI     NruI     NsiI     PacI     PmlI    PspXI
     PsrI    RsrII    SexAI     SwaI
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