(Circular) (Six-base) MAPSORT of: pFN21AA1531.seq Check: 540 from: 1 to: 8068 pFN21AA1531 8068 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2604 3513 2604 Size: 909 7159 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5295 5878 278 Size: 53 83 186 936 3759 583 2468 Fragments arranged by size: 3759 2468 936 583 186 83 53 AccI GT'mk_AC Cuts at: 1937 3435 3672 5411 1937 Size: 1498 237 1739 4594 Fragments arranged by size: 4594 1739 1498 237 Acc65I G'GTAC_C Cuts at: 2847 5389 2847 Size: 2542 5526 AclI AA'CG_TT Cuts at: 1563 6195 6568 1563 Size: 4632 373 3063 Fragments arranged by size: 4632 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2426 3082 6131 7515 7783 1632 Size: 794 656 3049 1384 268 1917 Fragments arranged by size: 3049 1917 1384 794 656 268 AfeI AGC'GCT Cuts at: 4481 4481 Size: 8068 AflII C'TTAA_G Cuts at: 820 1017 2549 820 Size: 197 1532 6339 Fragments arranged by size: 6339 1532 197 AflIII A'CryG_T Cuts at: 1203 6967 7944 1203 Size: 5764 977 1327 Fragments arranged by size: 5764 1327 977 AgeI A'CCGG_T Cuts at: 6899 6899 Size: 8068 AhdI GACnn_n'nnGTC Cuts at: 6797 6797 Size: 8068 AleI CACnn'nnGTG Cuts at: 1166 3164 3278 3293 4560 1166 Size: 1998 114 15 1267 4674 Fragments arranged by size: 4674 1998 1267 114 15 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3503 3704 3893 1184 Size: 2319 201 189 5359 Fragments arranged by size: 5359 2319 201 189 AlwNI CAG_nnn'CTG Cuts at: 2689 5811 7383 2689 Size: 3122 1572 3374 Fragments arranged by size: 3374 3122 1572 ApaI G_GGCC'C Cuts at: 2620 2731 4368 4648 4695 4751 2620 Size: 111 1637 280 47 56 5937 Fragments arranged by size: 5937 1637 280 111 56 47 ApaLI G'TGCA_C Cuts at: 2986 4880 6125 7281 2986 Size: 1894 1245 1156 3773 Fragments arranged by size: 3773 1894 1245 1156 ApoI r'AATT_y Cuts at: 1493 3419 5377 5603 5755 7864 1493 Size: 1926 1958 226 152 2109 1697 Fragments arranged by size: 2109 1958 1926 1697 226 152 AscI GG'CGCG_CC Cuts at: 2103 4594 2103 Size: 2491 5577 AseI AT'TA_AT Cuts at: 160 1032 6622 160 Size: 872 5590 1606 Fragments arranged by size: 5590 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8068 AvaI C'yCGr_G Cuts at: 1870 1943 2090 2097 2143 2234 3187 3195 4361 Size: 73 147 7 46 91 953 8 1166 Cuts at: 4361 4491 4641 4845 5065 5393 1870 Size: 130 150 204 220 328 4545 Fragments arranged by size: 4545 1166 953 328 220 204 150 147 130 91 73 46 8 7 BamHI G'GATC_C Cuts at: 5398 5765 5398 Size: 367 7701 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2009 2035 2086 2258 2381 Size: 325 217 627 222 26 51 172 123 Cuts at: 2381 2567 2847 3182 3191 3554 3644 3955 4130 Size: 186 280 335 9 363 90 311 175 Cuts at: 4130 4841 5069 5264 5389 6844 618 Size: 711 228 195 125 1455 1842 Fragments arranged by size: 1842 1455 711 627 363 335 325 311 280 228 222 217 195 186 175 172 125 123 90 51 26 9 BanII G_rGCy'C Cuts at: 1391 2241 2301 2620 2731 2863 3945 4368 4465 Size: 850 60 319 111 132 1082 423 97 Cuts at: 4465 4648 4695 4751 5097 5387 1391 Size: 183 47 56 346 290 4072 Fragments arranged by size: 4072 1082 850 423 346 319 290 183 132 111 97 60 56 47 BbeI G_GCGC'C Cuts at: 2013 2039 2090 2385 3648 4134 5073 5268 2013 Size: 26 51 295 1263 486 939 195 4813 Fragments arranged by size: 4813 1263 939 486 295 195 51 26 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4540 5342 928 Size: 971 2641 802 3654 Fragments arranged by size: 3654 2641 971 802 BbvCI CC'TCA_GC Cuts at: 3584 4271 3584 Size: 687 7381 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3059 3093 3529 3563 6259 6293 1903 Size: 34 1122 34 436 34 2696 34 3678 Fragments arranged by size: 3678 2696 1122 436 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3162 5960 7170 3162 Size: 2798 1210 4060 Fragments arranged by size: 4060 2798 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8068 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2161 4451 4460 Size: 107 122 71 631 605 489 2290 9 Cuts at: 4460 5160 5262 6679 136 Size: 700 102 1417 1525 Fragments arranged by size: 2290 1525 1417 700 631 605 489 122 107 102 71 9 BglII A'GATC_T Cuts at: 2416 8063 2416 Size: 5647 2421 BlpI GC'TnA_GC Cuts at: 2165 3617 5485 2165 Size: 1452 1868 4748 Fragments arranged by size: 4748 1868 1452 Bme1580I G_kGCm'C Cuts at: 1790 2570 2620 2674 2731 2990 3171 3187 3194 Size: 780 50 54 57 259 181 16 7 Cuts at: 3194 3559 3958 4368 4648 4695 4751 4844 4884 Size: 365 399 410 280 47 56 93 40 Cuts at: 4884 6129 7285 1790 Size: 1245 1156 2573 Fragments arranged by size: 2573 1245 1156 780 410 399 365 280 259 181 93 57 56 54 50 47 40 16 7 BmgBI CAC'GTC Cuts at: 1318 4579 1318 Size: 3261 4807 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2661 3990 4347 Size: 416 53 83 399 24 1244 1329 357 Cuts at: 4347 5799 6757 442 Size: 1452 958 1753 Fragments arranged by size: 1753 1452 1329 1244 958 416 399 357 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3372 1056 Size: 2316 5752 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2354 2494 2684 2753 3062 3581 3970 4047 Size: 1115 140 190 69 309 519 389 77 Cuts at: 4047 4274 5009 5231 6728 1239 Size: 227 735 222 1497 2579 Fragments arranged by size: 2579 1497 1115 735 519 389 309 227 222 190 140 77 69 Bpu10I CC'TnA_GC Cuts at: 2682 3584 4271 4289 2682 Size: 902 687 18 6461 Fragments arranged by size: 6461 902 687 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2296 4688 4940 5177 6193 7058 7356 2296 Size: 2392 252 237 1016 865 298 3008 Fragments arranged by size: 3008 2392 1016 865 298 252 237 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2447 3038 6731 882 Size: 617 948 591 3693 2219 Fragments arranged by size: 3693 2219 948 617 591 BsaAI yAC'GTr Cuts at: 493 1204 5859 493 Size: 711 4655 2702 Fragments arranged by size: 4655 2702 711 BsaBI GATnn'nnATC Cuts at: 3305 8062 3305 Size: 4757 3311 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2010 2036 2087 2175 Size: 53 83 186 936 477 26 51 88 Cuts at: 2175 2382 3645 4117 4131 4358 4891 5070 5265 Size: 207 1263 472 14 227 533 179 195 Cuts at: 5265 5292 5875 6257 6892 275 Size: 27 583 382 635 1451 Fragments arranged by size: 1451 1263 936 635 583 533 477 472 382 227 207 195 186 179 88 83 53 51 27 26 14 BsaWI w'CCGG_w Cuts at: 2430 4008 5339 6501 6899 7173 7320 7731 2430 Size: 1578 1331 1162 398 274 147 411 2767 Fragments arranged by size: 2767 1578 1331 1162 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2492 2522 3725 3755 3872 Size: 30 165 30 1089 30 1203 30 117 Cuts at: 3872 3902 4982 5012 1178 Size: 30 1080 30 4234 Fragments arranged by size: 4234 1203 1089 1080 165 117 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3877 4280 1186 Size: 2691 403 4974 Fragments arranged by size: 4974 2691 403 BseYI C'CCAG_C Cuts at: 2709 3228 3519 3597 4476 7271 7767 2709 Size: 519 291 78 879 2795 496 3010 Fragments arranged by size: 3010 2795 879 519 496 291 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2227 2393 2723 2905 3644 3664 3863 Size: 573 491 166 330 182 739 20 199 Cuts at: 3863 4522 5003 1163 Size: 659 481 4228 Fragments arranged by size: 4228 739 659 573 491 481 330 199 182 166 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 4812 5518 6279 7307 1540 Size: 275 186 1225 1586 706 761 1028 2301 Fragments arranged by size: 2301 1586 1225 1028 761 706 275 186 BsiHKAI G_wGCw'C Cuts at: 2144 2810 2990 3504 3933 3945 4884 6129 6214 Size: 666 180 514 429 12 939 1245 85 Cuts at: 6214 7285 2144 Size: 1071 2927 Fragments arranged by size: 2927 1245 1071 939 666 514 429 180 85 12 BsmI GAATG_Cn' Cuts at: 5579 5672 5579 Size: 93 7975 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2144 2241 2301 2570 2620 2674 2731 Size: 399 354 97 60 269 50 54 57 Cuts at: 2731 2810 2863 2990 3171 3187 3194 3504 3559 Size: 79 53 127 181 16 7 310 55 Cuts at: 3559 3933 3945 3958 4368 4465 4648 4695 4751 Size: 374 12 13 410 97 183 47 56 Cuts at: 4751 4844 4884 5097 5387 6129 6214 7285 1391 Size: 93 40 213 290 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 410 399 374 354 310 290 269 213 183 181 127 97 97 93 85 79 60 57 56 55 54 53 50 47 40 16 13 12 7 BspEI T'CCGG_A Cuts at: 7731 7731 Size: 8068 BspHI T'CATG_A Cuts at: 5957 7687 5957 Size: 1730 6338 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2604 3513 4776 5423 844 Size: 1760 909 1263 647 3489 Fragments arranged by size: 3489 1760 1263 909 647 BsrBI CCG'CTC Cuts at: 1394 1823 2152 2169 3983 5010 5232 5242 5955 Size: 429 329 17 1814 1027 222 10 713 Cuts at: 5955 1394 Size: 3507 Fragments arranged by size: 3507 1814 1027 713 429 329 222 17 10 BsrDI GCAATG_nn' Cuts at: 65 1248 2311 6563 6737 65 Size: 1183 1063 4252 174 1396 Fragments arranged by size: 4252 1396 1183 1063 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3207 3221 4811 4834 4950 5072 6712 Size: 110 1397 14 1590 23 116 122 1640 Cuts at: 6712 6899 1700 Size: 187 2869 Fragments arranged by size: 2869 1640 1590 1397 187 122 116 110 23 14 BsrGI T'GTAC_A Cuts at: 96 3285 3351 96 Size: 3189 66 4813 Fragments arranged by size: 4813 3189 66 BssHII G'CGCG_C Cuts at: 1925 2042 2103 4344 4594 5205 1925 Size: 117 61 2241 250 611 4788 Fragments arranged by size: 4788 2241 611 250 117 61 BssSI C'ACGA_G Cuts at: 4588 6128 7140 4588 Size: 1540 1012 5516 Fragments arranged by size: 5516 1540 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3775 794 Size: 2981 5087 BstBI TT'CG_AA Cuts at: 6872 6872 Size: 8068 BstEII G'GTnAC_C Cuts at: 1184 4420 1184 Size: 3236 4832 BstXI CCAn_nnnn'nTGG Cuts at: 2821 3637 2821 Size: 816 7252 BstYI r'GATC_y Cuts at: 1972 2416 4173 5398 5765 6150 6167 7619 7705 Size: 444 1757 1225 367 385 17 1452 86 Cuts at: 7705 7717 8063 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1757 1452 1225 444 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3673 3673 Size: 8068 Bsu36I CC'TnA_GG Cuts at: 2348 2971 2348 Size: 623 7445 BtgI C'CryG_G Cuts at: 513 1065 2004 2066 2997 3088 3666 4620 4885 Size: 552 939 62 931 91 578 954 265 Cuts at: 4885 513 Size: 3696 Fragments arranged by size: 3696 954 939 931 578 552 265 91 62 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5210 487 Size: 683 56 3984 3345 Fragments arranged by size: 3984 3345 683 56 BtsI GCAGTG_nn' Cuts at: 2327 4476 5584 6378 6398 2327 Size: 2149 1108 794 20 3997 Fragments arranged by size: 3997 2149 1108 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8068 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8033 DraI TTT'AAA Cuts at: 5372 5372 Size: 8068 DraIII CAC_nnn'GTG Cuts at: 3667 3667 Size: 8068 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7075 809 Size: 507 5759 1802 Fragments arranged by size: 5759 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2898 3223 3474 Size: 54 1425 50 275 18 1068 325 251 Cuts at: 3474 4443 4729 4809 5515 6404 8033 8 Size: 969 286 80 706 889 1629 43 Fragments arranged by size: 1629 1425 1068 969 889 706 325 286 275 251 80 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 4809 5515 1537 Size: 275 1411 1586 706 4090 Fragments arranged by size: 4090 1586 1411 706 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3710 4547 5345 5998 1353 Size: 2357 837 798 653 3423 Fragments arranged by size: 3423 2357 837 798 653 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2209 4519 4585 6655 7029 7175 Size: 1064 138 620 2310 66 2070 374 146 Cuts at: 7175 387 Size: 1280 Fragments arranged by size: 2310 2070 1280 1064 620 374 146 138 66 EcoICRI GAG'CTC Cuts at: 3943 3943 Size: 8068 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2354 2426 2494 2684 2753 3062 3082 Size: 393 722 72 68 190 69 309 20 Cuts at: 3082 3581 3970 4047 4274 5009 5231 6131 6728 Size: 499 389 77 227 735 222 900 597 Cuts at: 6728 7515 7783 1239 Size: 787 268 1524 Fragments arranged by size: 1524 900 787 735 722 597 499 393 389 309 268 227 222 190 77 72 69 68 20 EcoNI CCTnn'n_nnAGG Cuts at: 2702 3628 2702 Size: 926 7142 EcoO109I rG'GnC_Cy Cuts at: 2218 2464 2686 2727 2728 3976 4208 4245 4365 Size: 246 222 41 1 1248 232 37 120 Cuts at: 4365 4645 4691 4692 4748 4960 2218 Size: 280 46 1 56 212 5326 Fragments arranged by size: 5326 1248 280 246 232 222 212 120 56 46 41 37 1 1 EcoRI G'AATT_C Cuts at: 5377 5755 5377 Size: 378 7690 FspI TGC'GCA Cuts at: 6574 6574 Size: 8068 HaeII r_GCGC'y Cuts at: 1678 2013 2039 2090 2385 3624 3648 4134 4418 Size: 335 26 51 295 1239 24 486 284 Cuts at: 4418 4483 4988 5073 5133 5170 5178 5268 7215 Size: 65 505 85 60 37 8 90 1947 Cuts at: 7215 1678 Size: 2531 Fragments arranged by size: 2531 1947 1239 505 486 335 295 284 90 85 65 60 51 37 26 24 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2425 2457 2935 2967 3319 Size: 32 135 32 853 32 478 32 352 Cuts at: 3319 3351 4982 4999 5014 5031 6709 6741 6783 Size: 32 1631 17 15 17 1678 32 42 Cuts at: 6783 6815 1373 Size: 32 2626 Fragments arranged by size: 2626 1678 1631 853 478 352 135 42 32 32 32 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 3436 4069 5412 5658 669 Size: 988 281 1498 633 1343 246 3079 Fragments arranged by size: 3079 1498 1343 988 633 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8068 HpaI GTT'AAC Cuts at: 5658 5658 Size: 8068 KasI G'GCGC_C Cuts at: 2009 2035 2086 2381 3644 4130 5069 5264 2009 Size: 26 51 295 1263 486 939 195 4813 Fragments arranged by size: 4813 1263 939 486 295 195 51 26 KpnI G_GTAC'C Cuts at: 2851 5393 2851 Size: 2542 5526 MfeI C'AATT_G Cuts at: 5667 5667 Size: 8068 MluI A'CGCG_T Cuts at: 7944 7944 Size: 8068 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2448 2506 2622 7182 7366 1305 Size: 1143 58 116 4560 184 2007 Fragments arranged by size: 4560 2007 1143 184 116 58 MscI TGG'CCA Cuts at: 10 64 1489 1832 3476 4445 10 Size: 54 1425 343 1644 969 3633 Fragments arranged by size: 3633 1644 1425 969 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2960 3164 3278 3293 3608 3749 3800 Size: 648 1794 204 114 15 315 141 51 Cuts at: 3800 4145 4560 5300 6026 6385 6544 518 Size: 345 415 740 726 359 159 2042 Fragments arranged by size: 2042 1794 740 726 648 415 359 345 315 204 159 141 114 51 15 MspA1I CmG'CkG Cuts at: 1606 2059 2133 2211 2638 2844 3090 3228 3343 Size: 453 74 78 427 206 246 138 115 Cuts at: 3343 4061 4299 4506 4622 4728 4738 4774 5083 Size: 718 238 207 116 106 10 36 309 Cuts at: 5083 5322 5484 6161 7309 7554 1606 Size: 239 162 677 1148 245 2120 Fragments arranged by size: 2120 1148 718 677 453 427 309 246 245 239 238 207 206 162 138 116 115 106 78 74 36 10 NaeI GCC'GGC Cuts at: 3223 4813 4836 5074 3223 Size: 1590 23 238 6217 Fragments arranged by size: 6217 1590 238 23 NarI GG'CG_CC Cuts at: 2010 2036 2087 2382 3645 4131 5070 5265 2010 Size: 26 51 295 1263 486 939 195 4813 Fragments arranged by size: 4813 1263 939 486 295 195 51 26 NcoI C'CATG_G Cuts at: 513 1065 2004 2997 513 Size: 552 939 993 5584 Fragments arranged by size: 5584 993 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8068 NgoMIV G'CCGG_C Cuts at: 3221 4811 4834 5072 3221 Size: 1590 23 238 6217 Fragments arranged by size: 6217 1590 238 23 NheI G'CTAG_C Cuts at: 1052 3368 1052 Size: 2316 5752 NotI GC'GGCC_GC Cuts at: 5515 5515 Size: 8068 NspI r_CATG'y Cuts at: 1132 1229 2681 3775 5426 6971 1132 Size: 97 1452 1094 1651 1545 2229 Fragments arranged by size: 2229 1651 1545 1452 1094 97 PasI CC'CwG_GG Cuts at: 1348 2613 2689 3172 3630 4534 1348 Size: 1265 76 483 458 904 4882 Fragments arranged by size: 4882 1265 904 483 458 76 PciI A'CATG_T Cuts at: 6967 6967 Size: 8068 PflMI CCAn_nnn'nTGG Cuts at: 1108 3865 4127 4738 1108 Size: 2757 262 611 4438 Fragments arranged by size: 4438 2757 611 262 PfoI T'CCnGG_A Cuts at: 2223 2700 3755 4396 2223 Size: 477 1055 641 5895 Fragments arranged by size: 5895 1055 641 477 PmeI GTTT'AAAC Cuts at: 5372 5372 Size: 8068 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2637 2669 6137 6169 7650 7682 2637 Size: 32 3468 32 1481 32 3023 Fragments arranged by size: 3468 3023 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2218 3976 4208 2218 Size: 1758 232 6078 Fragments arranged by size: 6078 1758 232 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8068 PsiI TTA'TAA Cuts at: 5638 5638 Size: 8068 PspOMI G'GGCC_C Cuts at: 2616 2727 4364 4644 4691 4747 2616 Size: 111 1637 280 47 56 5937 Fragments arranged by size: 5937 1637 280 111 56 47 PstI C_TGCA'G Cuts at: 830 2637 3150 3542 4066 4773 5082 5420 830 Size: 1807 513 392 524 707 309 338 3478 Fragments arranged by size: 3478 1807 707 524 513 392 338 309 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8068 PvuII CAG'CTG Cuts at: 2133 2638 3343 4061 4506 4774 5083 2133 Size: 505 705 718 445 268 309 5118 Fragments arranged by size: 5118 718 705 505 445 309 268 SacI G_AGCT'C Cuts at: 3945 3945 Size: 8068 SacII CC_GC'GG Cuts at: 3091 4623 3091 Size: 1532 6536 SalI G'TCGA_C Cuts at: 1936 3434 5410 1936 Size: 1498 1976 4594 Fragments arranged by size: 4594 1976 1498 SanDI GG'GwC_CC Cuts at: 3976 3976 Size: 8068 SapI GCTCTTCn'nnn_ Cuts at: 5345 5345 Size: 8068 SbfI CC_TGCA'GG Cuts at: 5420 5420 Size: 8068 ScaI AGT'ACT Cuts at: 6316 8028 6316 Size: 1712 6356 SfcI C'TryA_G Cuts at: 826 1045 2633 3146 3538 4062 4769 5078 5416 Size: 219 1588 513 392 524 707 309 338 Cuts at: 5416 6551 7232 7423 826 Size: 1135 681 191 1471 Fragments arranged by size: 1588 1471 1135 707 681 524 513 392 338 309 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4451 5160 4451 Size: 709 7359 SfoI GGC'GCC Cuts at: 2011 2037 2088 2383 3646 4132 5071 5266 2011 Size: 26 51 295 1263 486 939 195 4813 Fragments arranged by size: 4813 1263 939 486 295 195 51 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8068 SgrAI Cr'CCGG_yG Cuts at: 1700 3207 1700 Size: 1507 6561 SmaI CCC'GGG Cuts at: 1872 3189 4363 4493 4643 4847 5067 5395 1872 Size: 1317 1174 130 150 204 220 328 4545 Fragments arranged by size: 4545 1317 1174 328 220 204 150 130 SmlI C'TyrA_G Cuts at: 820 1017 1943 2275 2549 4703 4955 5192 6172 Size: 197 926 332 274 2154 252 237 980 Cuts at: 6172 7073 7335 820 Size: 901 262 1553 Fragments arranged by size: 2154 1553 980 926 901 332 274 262 252 237 197 SnaBI TAC'GTA Cuts at: 493 5859 493 Size: 5366 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8068 SphI G_CATG'C Cuts at: 1132 2681 5426 1132 Size: 1549 2745 3774 Fragments arranged by size: 3774 2745 1549 SrfI GCCC'GGGC Cuts at: 4363 5067 4363 Size: 704 7364 SspI AAT'ATT Cuts at: 5 52 5992 5 Size: 47 5940 2081 Fragments arranged by size: 5940 2081 47 StuI AGG'CCT Cuts at: 1517 3536 4937 1517 Size: 2019 1401 4648 Fragments arranged by size: 4648 2019 1401 StyI C'CwwG_G Cuts at: 513 1065 2004 2157 2997 3004 4126 4227 4914 Size: 552 939 153 840 7 1122 101 687 Cuts at: 4914 5271 5507 513 Size: 357 236 3074 Fragments arranged by size: 3074 1122 939 840 687 552 357 236 153 101 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3542 6095 6112 6265 6450 6895 325 Size: 1255 1962 2553 17 153 185 445 1498 Fragments arranged by size: 2553 1962 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3285 3351 4437 Size: 274 80 33 51 1443 1308 66 1086 Cuts at: 4437 6314 8026 96 Size: 1877 1712 138 Fragments arranged by size: 1877 1712 1443 1308 1086 274 138 80 66 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3086 3118 3872 3904 8028 8060 3086 Size: 32 754 32 4124 32 3094 Fragments arranged by size: 4124 3094 754 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2947 5291 1316 Size: 1631 2344 4093 Fragments arranged by size: 4093 2344 1631 XbaI T'CTAG_A Cuts at: 5404 5404 Size: 8068 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3022 3847 4771 3022 Size: 825 924 6319 Fragments arranged by size: 6319 924 825 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8068 XmaI C'CCGG_G Cuts at: 1870 3187 4361 4491 4641 4845 5065 5393 1870 Size: 1317 1174 130 150 204 220 328 4545 Fragments arranged by size: 4545 1317 1174 328 220 204 150 130 XmnI GAAnn'nnTTC Cuts at: 6197 6197 Size: 8068 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5293 5876 276 Size: 53 83 186 936 3759 583 2468 Fragments arranged by size: 3759 2468 936 583 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BfrBI BplI BsiWI BsmBI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SwaI