(Circular) (Six-base) MAPSORT of: pFN21AA1525.seq Check: 6602 from: 1 to: 8101 pFN21AA1525 8101 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4000 4000 Size: 8101 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4997 5911 278 Size: 53 83 186 936 3461 914 2468 Fragments arranged by size: 3461 2468 936 914 186 83 53 AccI GT'mk_AC Cuts at: 1937 2436 3002 4449 4683 5444 1937 Size: 499 566 1447 234 761 4594 Fragments arranged by size: 4594 1447 761 566 499 234 Acc65I G'GTAC_C Cuts at: 5422 5422 Size: 8101 AclI AA'CG_TT Cuts at: 1563 4153 6228 6601 1563 Size: 2590 2075 373 3063 Fragments arranged by size: 3063 2590 2075 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2751 3906 4731 5265 6164 7548 7816 1632 Size: 1119 1155 825 534 899 1384 268 1917 Fragments arranged by size: 1917 1384 1155 1119 899 825 534 268 AflII C'TTAA_G Cuts at: 820 1017 3601 820 Size: 197 2584 5320 Fragments arranged by size: 5320 2584 197 AflIII A'CryG_T Cuts at: 1203 2733 7000 7977 1203 Size: 1530 4267 977 1327 Fragments arranged by size: 4267 1530 1327 977 AgeI A'CCGG_T Cuts at: 5150 6932 5150 Size: 1782 6319 AhdI GACnn_n'nnGTC Cuts at: 5287 6830 5287 Size: 1543 6558 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8101 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3717 1184 Size: 2533 5568 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3375 3407 3375 Size: 32 8069 AlwNI CAG_nnn'CTG Cuts at: 2774 3994 4106 4574 4709 4738 5844 7416 2774 Size: 1220 112 468 135 29 1106 1572 3459 Fragments arranged by size: 3459 1572 1220 1106 468 135 112 29 ApaLI G'TGCA_C Cuts at: 3257 6158 7314 3257 Size: 2901 1156 4044 Fragments arranged by size: 4044 2901 1156 ApoI r'AATT_y Cuts at: 1493 2059 2681 2695 2872 3146 3290 3939 4065 Size: 566 622 14 177 274 144 649 126 Cuts at: 4065 4136 5410 5636 5788 7897 1493 Size: 71 1274 226 152 2109 1697 Fragments arranged by size: 2109 1697 1274 649 622 566 274 226 177 152 144 126 71 14 AseI AT'TA_AT Cuts at: 160 1032 6655 160 Size: 872 5623 1606 Fragments arranged by size: 5623 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 AvaI C'yCGr_G Cuts at: 1870 1943 2044 5426 1870 Size: 73 101 3382 4545 Fragments arranged by size: 4545 3382 101 73 BamHI G'GATC_C Cuts at: 5431 5798 5431 Size: 367 7734 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4046 5422 6877 618 Size: 325 217 627 2259 1376 1455 1842 Fragments arranged by size: 2259 1842 1455 1376 627 325 217 BanII G_rGCy'C Cuts at: 1391 2826 3531 5308 5420 1391 Size: 1435 705 1777 112 4072 Fragments arranged by size: 4072 1777 1435 705 112 BbeI G_GCGC'C Cuts at: 4050 4050 Size: 8101 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5278 928 Size: 971 3379 3751 Fragments arranged by size: 3751 3379 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6292 6326 1903 Size: 34 4355 34 3678 Fragments arranged by size: 4355 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2883 5993 7203 2883 Size: 3110 1210 3781 Fragments arranged by size: 3781 3110 1210 BclI T'GATC_A Cuts at: 1548 3879 1548 Size: 2331 5770 BfrBI ATG'CAT Cuts at: 2018 2303 2018 Size: 285 7816 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2363 2483 2837 Size: 107 122 71 631 605 691 120 354 Cuts at: 2837 6712 136 Size: 3875 1525 Fragments arranged by size: 3875 1525 691 631 605 354 122 120 107 71 BglII A'GATC_T Cuts at: 4243 8096 4243 Size: 3853 4248 BlpI GC'TnA_GC Cuts at: 5518 5518 Size: 8101 Bme1580I G_kGCm'C Cuts at: 1790 3261 6162 7318 1790 Size: 1471 2901 1156 2573 Fragments arranged by size: 2901 2573 1471 1156 BmgBI CAC'GTC Cuts at: 1318 3010 1318 Size: 1692 6409 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4353 4439 4511 Size: 416 53 83 399 24 2936 86 72 Cuts at: 4511 5832 6790 442 Size: 1321 958 1753 Fragments arranged by size: 2936 1753 1321 958 416 399 86 83 72 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8101 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2288 3211 2288 Size: 923 7178 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4399 6761 1239 Size: 3160 2362 2579 Fragments arranged by size: 3160 2579 2362 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2623 3206 4637 6226 7091 7389 2623 Size: 583 1431 1589 865 298 3335 Fragments arranged by size: 3335 1589 1431 865 583 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6764 882 Size: 617 5265 2219 Fragments arranged by size: 5265 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 5892 493 Size: 711 4688 2702 Fragments arranged by size: 4688 2702 711 BsaBI GATnn'nnATC Cuts at: 8095 8095 Size: 8101 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4047 4994 5908 6290 Size: 53 83 186 936 2514 947 914 382 Cuts at: 6290 6925 275 Size: 635 1451 Fragments arranged by size: 2514 1451 947 936 914 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4831 5150 5264 6534 6932 7206 7353 7764 4831 Size: 319 114 1270 398 274 147 411 5168 Fragments arranged by size: 5168 1270 411 398 319 274 147 114 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3043 3073 1178 Size: 30 165 30 1640 30 6206 Fragments arranged by size: 6206 1640 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2261 3627 3947 1186 Size: 1075 1366 320 5340 Fragments arranged by size: 5340 1366 1075 320 BseYI C'CCAG_C Cuts at: 4306 4731 5106 7304 7800 4306 Size: 425 375 2198 496 4607 Fragments arranged by size: 4607 2198 496 425 375 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2835 4034 4455 1163 Size: 573 1099 1199 421 4809 Fragments arranged by size: 4809 1199 1099 573 421 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5551 6312 7340 1540 Size: 275 186 3550 761 1028 2301 Fragments arranged by size: 3550 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2826 3261 3531 5308 6162 6247 7318 2826 Size: 435 270 1777 854 85 1071 3609 Fragments arranged by size: 3609 1777 1071 854 435 270 85 BsmI GAATG_Cn' Cuts at: 5612 5705 5612 Size: 93 8008 BsmBI CGTCTCn'nnnn_ Cuts at: 3959 5001 3959 Size: 1042 7059 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2826 3261 3531 5308 5420 6162 6247 Size: 399 1036 435 270 1777 112 742 85 Cuts at: 6247 7318 1391 Size: 1071 2174 Fragments arranged by size: 2174 1777 1071 1036 742 435 399 270 112 85 BspEI T'CCGG_A Cuts at: 4831 7764 4831 Size: 2933 5168 BspHI T'CATG_A Cuts at: 3303 5990 7720 3303 Size: 2687 1730 3684 Fragments arranged by size: 3684 2687 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4000 5456 844 Size: 3156 1456 3489 Fragments arranged by size: 3489 3156 1456 BsrBI CCG'CTC Cuts at: 1394 1823 5988 1394 Size: 429 4165 3507 Fragments arranged by size: 4165 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2384 3180 3618 6596 6770 65 Size: 1183 1136 796 438 2978 174 1396 Fragments arranged by size: 2978 1396 1183 1136 796 438 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5150 6745 6932 1700 Size: 110 3340 1595 187 2869 Fragments arranged by size: 3340 2869 1595 187 110 BsrGI T'GTAC_A Cuts at: 96 4935 96 Size: 4839 3262 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8101 BssSI C'ACGA_G Cuts at: 6161 7173 6161 Size: 1012 7089 BstAPI GCAn_nnn'nTGC Cuts at: 794 3994 4106 794 Size: 3200 112 4789 Fragments arranged by size: 4789 3200 112 BstBI TT'CG_AA Cuts at: 2869 6905 2869 Size: 4036 4065 BstEII G'GTnAC_C Cuts at: 1184 5358 1184 Size: 4174 3927 BstYI r'GATC_y Cuts at: 1972 3537 3790 4243 5261 5431 5798 6183 6200 Size: 1565 253 453 1018 170 367 385 17 Cuts at: 6200 7652 7738 7750 8096 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1565 1452 1018 453 385 367 346 253 170 86 17 12 BstZ17I GTA'TAC Cuts at: 3003 3003 Size: 8101 BtgI C'CryG_G Cuts at: 513 1065 2004 2146 3114 5299 513 Size: 552 939 142 968 2185 3315 Fragments arranged by size: 3315 2185 968 939 552 142 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 4609 487 Size: 683 56 3383 3979 Fragments arranged by size: 3979 3383 683 56 BtsI GCAGTG_nn' Cuts at: 2510 3471 4491 5617 6411 6431 2510 Size: 961 1020 1126 794 20 4180 Fragments arranged by size: 4180 1126 1020 961 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8101 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8066 DraI TTT'AAA Cuts at: 2506 5405 2506 Size: 2899 5202 DraIII CAC_nnn'GTG Cuts at: 4961 4961 Size: 8101 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4359 7108 809 Size: 507 3043 2749 1802 Fragments arranged by size: 3043 2749 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2149 5548 6437 Size: 54 1425 50 275 18 319 3399 889 Cuts at: 6437 8066 8 Size: 1629 43 Fragments arranged by size: 3399 1629 1425 889 319 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5548 1537 Size: 275 3736 4090 Fragments arranged by size: 4090 3736 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2814 3519 3734 5250 6031 1353 Size: 1461 705 215 1516 781 3423 Fragments arranged by size: 3423 1516 1461 781 705 215 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6688 7062 7208 387 Size: 1064 138 5099 374 146 1280 Fragments arranged by size: 5099 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2824 3529 5306 2824 Size: 705 1777 5619 Fragments arranged by size: 5619 1777 705 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2751 3906 4399 4731 5265 6164 6761 Size: 393 1119 1155 493 332 534 899 597 Cuts at: 6761 7548 7816 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1155 1119 899 787 597 534 493 393 332 268 EcoNI CCTnn'n_nnAGG Cuts at: 3284 4908 3284 Size: 1624 6477 EcoO109I rG'GnC_Cy Cuts at: 2088 3511 4020 4724 2088 Size: 1423 509 704 5465 Fragments arranged by size: 5465 1423 704 509 EcoRI G'AATT_C Cuts at: 5410 5788 5410 Size: 378 7723 EcoRV GAT'ATC Cuts at: 2659 3178 3391 2659 Size: 519 213 7369 Fragments arranged by size: 7369 519 213 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3410 3936 5392 3410 Size: 526 1456 6119 Fragments arranged by size: 6119 1456 526 FspI TGC'GCA Cuts at: 6607 6607 Size: 8101 HaeII r_GCGC'y Cuts at: 1678 4050 7248 1678 Size: 2372 3198 2531 Fragments arranged by size: 3198 2531 2372 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2312 2344 3220 3252 3619 Size: 32 135 32 740 32 876 32 367 Cuts at: 3619 3651 4235 4267 5014 5046 5273 5305 6742 Size: 32 584 32 747 32 227 32 1437 Cuts at: 6742 6774 6816 6848 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1437 876 747 740 584 367 227 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5445 5691 669 Size: 988 281 3507 246 3079 Fragments arranged by size: 3507 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2787 3267 748 Size: 2039 480 5582 Fragments arranged by size: 5582 2039 480 HpaI GTT'AAC Cuts at: 5691 5691 Size: 8101 KasI G'GCGC_C Cuts at: 4046 4046 Size: 8101 KpnI G_GTAC'C Cuts at: 5426 5426 Size: 8101 MfeI C'AATT_G Cuts at: 5700 5700 Size: 8101 MluI A'CGCG_T Cuts at: 7977 7977 Size: 8101 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2204 7215 7399 1305 Size: 899 5011 184 2007 Fragments arranged by size: 5011 2007 899 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2151 10 Size: 54 1425 343 319 5960 Fragments arranged by size: 5960 1425 343 319 54 MslI CAynn'nnrTG Cuts at: 518 1166 2151 3240 4628 5085 6059 6418 6577 Size: 648 985 1089 1388 457 974 359 159 Cuts at: 6577 518 Size: 2042 Fragments arranged by size: 2042 1388 1089 985 974 648 457 359 159 MspA1I CmG'CkG Cuts at: 1606 2567 5110 5517 6194 7342 7587 1606 Size: 961 2543 407 677 1148 245 2120 Fragments arranged by size: 2543 2120 1148 961 677 407 245 NarI GG'CG_CC Cuts at: 4047 4047 Size: 8101 NcoI C'CATG_G Cuts at: 513 1065 2004 2146 3114 5299 513 Size: 552 939 142 968 2185 3315 Fragments arranged by size: 3315 2185 968 939 552 142 NdeI CA'TA_TG Cuts at: 387 3241 387 Size: 2854 5247 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8101 NotI GC'GGCC_GC Cuts at: 5548 5548 Size: 8101 NsiI A_TGCA'T Cuts at: 2020 2305 2020 Size: 285 7816 NspI r_CATG'y Cuts at: 1132 1229 2737 3157 5238 5459 7004 1132 Size: 97 1508 420 2081 221 1545 2229 Fragments arranged by size: 2229 2081 1545 1508 420 221 97 PasI CC'CwG_GG Cuts at: 1348 5293 1348 Size: 3945 4156 PciI A'CATG_T Cuts at: 2733 7000 2733 Size: 4267 3834 PflMI CCAn_nnn'nTGG Cuts at: 1108 2534 1108 Size: 1426 6675 PmeI GTTT'AAAC Cuts at: 5405 5405 Size: 8101 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6170 6202 7683 7715 6170 Size: 32 1481 32 6556 Fragments arranged by size: 6556 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2088 3511 2088 Size: 1423 6678 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8101 PsiI TTA'TAA Cuts at: 5671 5671 Size: 8101 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3564 3596 3564 Size: 32 8069 PstI C_TGCA'G Cuts at: 830 3990 4114 4228 4498 5182 5453 830 Size: 3160 124 114 270 684 271 3478 Fragments arranged by size: 3478 3160 684 271 270 124 114 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8101 PvuII CAG'CTG Cuts at: 2567 2567 Size: 8101 SacI G_AGCT'C Cuts at: 2826 3531 5308 2826 Size: 705 1777 5619 Fragments arranged by size: 5619 1777 705 SalI G'TCGA_C Cuts at: 1936 5443 1936 Size: 3507 4594 SapI GCTCTTCn'nnn_ Cuts at: 2814 3519 2814 Size: 705 7396 SbfI CC_TGCA'GG Cuts at: 5453 5453 Size: 8101 ScaI AGT'ACT Cuts at: 3634 6349 8061 3634 Size: 2715 1712 3674 Fragments arranged by size: 3674 2715 1712 SexAI A'CCwGG_T Cuts at: 4984 4984 Size: 8101 SfcI C'TryA_G Cuts at: 826 1045 3034 3371 3986 4110 4224 4494 4680 Size: 219 1989 337 615 124 114 270 186 Cuts at: 4680 5178 5449 6584 7265 7456 826 Size: 498 271 1135 681 191 1471 Fragments arranged by size: 1989 1471 1135 681 615 498 337 271 270 219 191 186 124 114 SfoI GGC'GCC Cuts at: 4048 4048 Size: 8101 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8101 SmaI CCC'GGG Cuts at: 1872 5428 1872 Size: 3556 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2638 3221 3601 4652 6205 7106 Size: 197 926 695 583 380 1051 1553 901 Cuts at: 7106 7368 820 Size: 262 1553 Fragments arranged by size: 1553 1553 1051 926 901 695 583 380 262 197 SnaBI TAC'GTA Cuts at: 493 5892 493 Size: 5399 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8101 SphI G_CATG'C Cuts at: 1132 5459 1132 Size: 4327 3774 SspI AAT'ATT Cuts at: 5 52 2445 6025 5 Size: 47 2393 3580 2081 Fragments arranged by size: 3580 2393 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8101 StyI C'CwwG_G Cuts at: 513 1065 2004 2146 3114 5299 5540 513 Size: 552 939 142 968 2185 241 3074 Fragments arranged by size: 3074 2185 968 939 552 241 142 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 1997 2268 2931 3244 4622 6128 6145 Size: 1255 417 271 663 313 1378 1506 17 Cuts at: 6145 6298 6483 6928 325 Size: 153 185 445 1498 Fragments arranged by size: 1506 1498 1378 1255 663 445 417 313 271 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2415 2426 3632 Size: 274 80 33 51 1443 438 11 1206 Cuts at: 3632 4214 4935 6347 8059 96 Size: 582 721 1412 1712 138 Fragments arranged by size: 1712 1443 1412 1206 721 582 438 274 138 80 51 33 11 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3043 3075 8061 8093 3043 Size: 32 4986 32 3051 Fragments arranged by size: 4986 3051 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3974 1316 Size: 2658 5443 XbaI T'CTAG_A Cuts at: 5437 5437 Size: 8101 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8101 XmaI C'CCGG_G Cuts at: 1870 5426 1870 Size: 3556 4545 XmnI GAAnn'nnTTC Cuts at: 6230 6230 Size: 8101 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4995 5909 276 Size: 53 83 186 936 3461 914 2468 Fragments arranged by size: 3461 2468 936 914 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI Bpu10I BsiWI BstXI Bsu36I FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI RsrII SacII SanDI SfiI SrfI SwaI XcmI