(Circular) (Six-base) MAPSORT of: pFN21AA1520.seq Check: 8339 from: 1 to: 7003 pFN21AA1520 7003 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3184 3184 Size: 7003 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4813 278 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3399 4346 1937 Size: 1462 947 4594 Fragments arranged by size: 4594 1462 947 Acc65I G'GTAC_C Cuts at: 4324 4324 Size: 7003 AclI AA'CG_TT Cuts at: 1563 5130 5503 1563 Size: 3567 373 3063 Fragments arranged by size: 3567 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2068 3568 4223 5066 6450 6718 1632 Size: 436 1500 655 843 1384 268 1917 Fragments arranged by size: 1917 1500 1384 843 655 436 268 AfeI AGC'GCT Cuts at: 4018 4018 Size: 7003 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6806 AflIII A'CryG_T Cuts at: 1203 3231 5902 6879 1203 Size: 2028 2671 977 1327 Fragments arranged by size: 2671 2028 1327 977 AgeI A'CCGG_T Cuts at: 5834 5834 Size: 7003 AhdI GACnn_n'nnGTC Cuts at: 5732 5732 Size: 7003 AleI CACnn'nnGTG Cuts at: 1166 2267 2951 1166 Size: 1101 684 5218 Fragments arranged by size: 5218 1101 684 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2198 1184 Size: 1014 5989 AlwNI CAG_nnn'CTG Cuts at: 2443 2533 3568 4183 4186 4231 4746 6318 2443 Size: 90 1035 615 3 45 515 1572 3128 Fragments arranged by size: 3128 1572 1035 615 515 90 45 3 ApaI G_GGCC'C Cuts at: 2232 2359 3624 2232 Size: 127 1265 5611 Fragments arranged by size: 5611 1265 127 ApaLI G'TGCA_C Cuts at: 3473 5060 6216 3473 Size: 1587 1156 4260 Fragments arranged by size: 4260 1587 1156 ApoI r'AATT_y Cuts at: 1493 4312 4538 4690 6799 1493 Size: 2819 226 152 2109 1697 Fragments arranged by size: 2819 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5557 160 Size: 872 4525 1606 Fragments arranged by size: 4525 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 AvaI C'yCGr_G Cuts at: 1870 1943 2501 3625 3966 4328 1870 Size: 73 558 1124 341 362 4545 Fragments arranged by size: 4545 1124 558 362 341 73 BamHI G'GATC_C Cuts at: 4333 4700 4333 Size: 367 6636 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2432 3923 4169 4324 5779 Size: 325 217 627 645 1491 246 155 1455 Cuts at: 5779 618 Size: 1842 Fragments arranged by size: 1842 1491 1455 645 627 325 246 217 155 BanII G_rGCy'C Cuts at: 1391 2232 2359 3258 3395 3624 3644 3771 3973 Size: 841 127 899 137 229 20 127 202 Cuts at: 3973 4322 1391 Size: 349 4072 Fragments arranged by size: 4072 899 841 349 229 202 137 127 127 20 BbeI G_GCGC'C Cuts at: 3927 3927 Size: 7003 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2074 2914 2953 3137 928 Size: 971 175 840 39 184 4794 Fragments arranged by size: 4794 971 840 184 175 39 BbvCI CC'TCA_GC Cuts at: 2469 2469 Size: 7003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2671 2705 3322 3356 3454 Size: 34 50 34 650 34 617 34 98 Cuts at: 3454 3488 5194 5228 1903 Size: 34 1706 34 3678 Fragments arranged by size: 3678 1706 650 617 98 50 34 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4895 6105 4895 Size: 1210 5793 BclI T'GATC_A Cuts at: 1548 1548 Size: 7003 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2236 2465 5614 Size: 107 122 71 631 605 564 229 3149 Cuts at: 5614 136 Size: 1525 Fragments arranged by size: 3149 1525 631 605 564 229 122 107 71 BglII A'GATC_T Cuts at: 6998 6998 Size: 7003 BlpI GC'TnA_GC Cuts at: 4106 4420 4106 Size: 314 6689 Bme1580I G_kGCm'C Cuts at: 1790 2232 2359 3477 3624 4172 5064 6220 1790 Size: 442 127 1118 147 548 892 1156 2573 Fragments arranged by size: 2573 1156 1118 892 548 442 147 127 BmgBI CAC'GTC Cuts at: 1318 2557 1318 Size: 1239 5764 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3983 4734 5692 Size: 416 53 83 399 24 2566 751 958 Cuts at: 5692 442 Size: 1753 Fragments arranged by size: 2566 1753 958 751 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7003 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2465 3524 3680 3698 3872 5663 1239 Size: 890 336 1059 156 18 174 1791 2579 Fragments arranged by size: 2579 1791 1059 890 336 174 156 18 Bpu10I CC'TnA_GC Cuts at: 2469 2469 Size: 7003 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5128 5993 6291 5128 Size: 865 298 5840 Fragments arranged by size: 5840 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2603 2908 3107 5666 882 Size: 617 1104 305 199 2559 2219 Fragments arranged by size: 2559 2219 1104 617 305 199 BsaAI yAC'GTr Cuts at: 493 1204 4794 493 Size: 711 3590 2702 Fragments arranged by size: 3590 2702 711 BsaBI GATnn'nnATC Cuts at: 6997 6997 Size: 7003 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2510 3924 4810 5192 Size: 53 83 186 936 977 1414 886 382 Cuts at: 5192 5827 275 Size: 635 1451 Fragments arranged by size: 1451 1414 977 936 886 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3138 5436 5834 6108 6255 6666 3138 Size: 2298 398 274 147 411 3475 Fragments arranged by size: 3475 2298 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2846 2876 2888 2918 1178 Size: 30 165 30 1443 30 12 30 5263 Fragments arranged by size: 5263 1443 165 30 30 30 30 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3125 3170 3173 3176 1186 Size: 1939 45 3 3 5013 Fragments arranged by size: 5013 1939 45 3 3 BseYI C'CCAG_C Cuts at: 2188 2224 3927 4231 6206 6702 2188 Size: 36 1703 304 1975 496 2489 Fragments arranged by size: 2489 1975 1703 496 304 36 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4181 4238 1163 Size: 573 2445 57 3928 Fragments arranged by size: 3928 2445 573 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2491 3457 4453 5214 6242 1540 Size: 275 186 490 966 996 761 1028 2301 Fragments arranged by size: 2301 1028 996 966 761 490 275 186 BsiHKAI G_wGCw'C Cuts at: 3477 3644 4112 4122 5064 5149 6220 3477 Size: 167 468 10 942 85 1071 4260 Fragments arranged by size: 4260 1071 942 468 167 85 10 BsmI GAATG_Cn' Cuts at: 3085 4514 4607 3085 Size: 1429 93 5481 Fragments arranged by size: 5481 1429 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2232 2359 3258 3395 3477 3624 3644 Size: 399 442 127 899 137 82 147 20 Cuts at: 3644 3771 3973 4112 4122 4172 4322 5064 5149 Size: 127 202 139 10 50 150 742 85 Cuts at: 5149 6220 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 899 742 442 399 202 150 147 139 137 127 127 85 82 50 20 10 BspEI T'CCGG_A Cuts at: 3138 6666 3138 Size: 3528 3475 BspHI T'CATG_A Cuts at: 2043 3021 4892 6622 2043 Size: 978 1871 1730 2424 Fragments arranged by size: 2424 1871 1730 978 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2723 3184 3186 4077 4358 844 Size: 1879 461 2 891 281 3489 Fragments arranged by size: 3489 1879 891 461 281 2 BsrBI CCG'CTC Cuts at: 1394 1823 2487 2805 3786 4890 1394 Size: 429 664 318 981 1104 3507 Fragments arranged by size: 3507 1104 981 664 429 318 BsrDI GCAATG_nn' Cuts at: 65 1248 5498 5672 65 Size: 1183 4250 174 1396 Fragments arranged by size: 4250 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2490 3456 4101 5647 5834 1700 Size: 110 680 966 645 1546 187 2869 Fragments arranged by size: 2869 1546 966 680 645 187 110 BsrGI T'GTAC_A Cuts at: 96 3198 96 Size: 3102 3901 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7003 BssSI C'ACGA_G Cuts at: 2248 2267 2567 5063 6075 2248 Size: 19 300 2496 1012 3176 Fragments arranged by size: 3176 2496 1012 300 19 BstAPI GCAn_nnn'nTGC Cuts at: 794 2533 794 Size: 1739 5264 BstBI TT'CG_AA Cuts at: 2788 5807 2788 Size: 3019 3984 BstEII G'GTnAC_C Cuts at: 1184 2995 1184 Size: 1811 5192 BstXI CCAn_nnnn'nTGG Cuts at: 2206 4021 2206 Size: 1815 5188 BstYI r'GATC_y Cuts at: 1972 2149 4333 4700 5085 5102 6554 6640 6652 Size: 177 2184 367 385 17 1452 86 12 Cuts at: 6652 6998 1972 Size: 346 1977 Fragments arranged by size: 2184 1977 1452 385 367 346 177 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2067 2295 2886 3387 3468 3868 3960 Size: 552 1002 228 591 501 81 400 92 Cuts at: 3960 4060 4113 513 Size: 100 53 3403 Fragments arranged by size: 3403 1002 591 552 501 400 228 100 92 81 53 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3325 4078 487 Size: 683 56 2099 753 3412 Fragments arranged by size: 3412 2099 753 683 56 BtsI GCAGTG_nn' Cuts at: 3260 3637 4519 5313 5333 3260 Size: 377 882 794 20 4930 Fragments arranged by size: 4930 882 794 377 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7003 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6968 DraI TTT'AAA Cuts at: 4307 4307 Size: 7003 DraIII CAC_nnn'GTG Cuts at: 3745 3745 Size: 7003 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6010 809 Size: 507 4694 1802 Fragments arranged by size: 4694 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2070 2208 2426 Size: 54 1425 50 275 18 240 138 218 Cuts at: 2426 2457 2488 2492 2571 2709 3087 3940 3957 Size: 31 31 4 79 138 378 853 17 Cuts at: 3957 4264 4450 5339 6968 8 Size: 307 186 889 1629 43 Fragments arranged by size: 1629 1425 889 853 378 307 275 240 218 186 138 138 79 54 50 43 31 31 18 17 4 EagI C'GGCC_G Cuts at: 1537 1812 2488 4450 1537 Size: 275 676 1962 4090 Fragments arranged by size: 4090 1962 676 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2282 2318 2804 3632 4933 1353 Size: 929 36 486 828 1301 3423 Fragments arranged by size: 3423 1301 929 828 486 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2108 3122 5590 5964 6110 387 Size: 1064 138 519 1014 2468 374 146 1280 Fragments arranged by size: 2468 1280 1064 1014 519 374 146 138 EcoICRI GAG'CTC Cuts at: 3642 3642 Size: 7003 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2068 2129 2465 3524 3568 3680 3698 Size: 393 436 61 336 1059 44 112 18 Cuts at: 3698 3872 4223 5066 5663 6450 6718 1239 Size: 174 351 843 597 787 268 1524 Fragments arranged by size: 1524 1059 843 787 597 436 393 351 336 268 174 112 61 44 18 EcoNI CCTnn'n_nnAGG Cuts at: 2108 3413 2108 Size: 1305 5698 EcoO109I rG'GnC_Cy Cuts at: 2228 2229 2341 2356 2440 2854 3565 3620 3621 Size: 1 112 15 84 414 711 55 1 Cuts at: 3621 2228 Size: 5610 Fragments arranged by size: 5610 711 414 112 84 55 15 1 1 EcoRI G'AATT_C Cuts at: 4312 4690 4312 Size: 378 6625 FseI GG_CCGG'CC Cuts at: 2494 2494 Size: 7003 FspI TGC'GCA Cuts at: 2327 5509 2327 Size: 3182 3821 HaeII r_GCGC'y Cuts at: 1678 3720 3927 4020 6150 1678 Size: 2042 207 93 2130 2531 Fragments arranged by size: 2531 2130 2042 207 93 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2495 2527 2846 2878 2888 Size: 32 135 32 923 32 319 32 10 Cuts at: 2888 2920 3788 3820 4024 4056 5644 5676 5718 Size: 32 868 32 204 32 1588 32 42 Cuts at: 5718 5750 1373 Size: 32 2626 Fragments arranged by size: 2626 1588 923 868 319 204 135 42 32 32 32 32 32 32 32 32 32 10 HincII GTy'rAC Cuts at: 669 1657 1938 3652 4347 4593 669 Size: 988 281 1714 695 246 3079 Fragments arranged by size: 3079 1714 988 695 281 246 HindIII A'AGCT_T Cuts at: 748 2827 748 Size: 2079 4924 HpaI GTT'AAC Cuts at: 4593 4593 Size: 7003 KasI G'GCGC_C Cuts at: 3923 3923 Size: 7003 KpnI G_GTAC'C Cuts at: 4328 4328 Size: 7003 MfeI C'AATT_G Cuts at: 4602 4602 Size: 7003 MluI A'CGCG_T Cuts at: 6879 6879 Size: 7003 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3057 6117 6301 1305 Size: 1752 3060 184 2007 Fragments arranged by size: 3060 2007 1752 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 2711 3089 3942 3959 Size: 54 1425 343 378 501 378 853 17 Cuts at: 3959 4266 10 Size: 307 2747 Fragments arranged by size: 2747 1425 853 501 378 378 343 307 54 17 MslI CAynn'nnrTG Cuts at: 518 1166 2048 2060 2204 2267 2885 2951 3002 Size: 648 882 12 144 63 618 66 51 Cuts at: 3002 3020 3023 3026 4136 4961 5320 5479 518 Size: 18 3 3 1110 825 359 159 2042 Fragments arranged by size: 2042 1110 882 825 648 618 359 159 144 66 63 51 18 12 3 3 MspA1I CmG'CkG Cuts at: 1606 2178 3224 3252 3330 3931 3952 4183 4222 Size: 572 1046 28 78 601 21 231 39 Cuts at: 4222 4262 4419 5096 6244 6489 1606 Size: 40 157 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 677 601 572 245 231 157 78 40 39 28 21 NaeI GCC'GGC Cuts at: 2492 2492 Size: 7003 NarI GG'CG_CC Cuts at: 3924 3924 Size: 7003 NcoI C'CATG_G Cuts at: 513 1065 2295 2886 3468 3960 4060 513 Size: 552 1230 591 582 492 100 3456 Fragments arranged by size: 3456 1230 591 582 552 492 100 NdeI CA'TA_TG Cuts at: 387 387 Size: 7003 NgoMIV G'CCGG_C Cuts at: 2490 2490 Size: 7003 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7003 NotI GC'GGCC_GC Cuts at: 4450 4450 Size: 7003 NspI r_CATG'y Cuts at: 1132 1229 3235 4361 5906 1132 Size: 97 2006 1126 1545 2229 Fragments arranged by size: 2229 2006 1545 1126 97 PasI CC'CwG_GG Cuts at: 1348 2480 4192 1348 Size: 1132 1712 4159 Fragments arranged by size: 4159 1712 1132 PciI A'CATG_T Cuts at: 3231 5902 3231 Size: 2671 4332 PflMI CCAn_nnn'nTGG Cuts at: 1108 2443 1108 Size: 1335 5668 PfoI T'CCnGG_A Cuts at: 2106 2337 3657 3888 3999 2106 Size: 231 1320 231 111 5110 Fragments arranged by size: 5110 1320 231 231 111 PmeI GTTT'AAAC Cuts at: 4307 4307 Size: 7003 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3425 3457 5072 5104 6585 6617 3425 Size: 32 1615 32 1481 32 3811 Fragments arranged by size: 3811 1615 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2341 2854 3565 2341 Size: 513 711 5779 Fragments arranged by size: 5779 711 513 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7003 PsiI TTA'TAA Cuts at: 4573 4573 Size: 7003 PspOMI G'GGCC_C Cuts at: 2228 2355 3620 2228 Size: 127 1265 5611 Fragments arranged by size: 5611 1265 127 PstI C_TGCA'G Cuts at: 830 2529 3192 3573 4074 4355 830 Size: 1699 663 381 501 281 3478 Fragments arranged by size: 3478 1699 663 501 381 281 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7003 PvuII CAG'CTG Cuts at: 2178 3224 3931 4183 4262 2178 Size: 1046 707 252 79 4919 Fragments arranged by size: 4919 1046 707 252 79 SacI G_AGCT'C Cuts at: 3644 3644 Size: 7003 SalI G'TCGA_C Cuts at: 1936 4345 1936 Size: 2409 4594 SanDI GG'GwC_CC Cuts at: 2341 2341 Size: 7003 SapI GCTCTTCn'nnn_ Cuts at: 2318 3632 2318 Size: 1314 5689 SbfI CC_TGCA'GG Cuts at: 4355 4355 Size: 7003 ScaI AGT'ACT Cuts at: 3050 3734 5251 6963 3050 Size: 684 1517 1712 3090 Fragments arranged by size: 3090 1712 1517 684 SexAI A'CCwGG_T Cuts at: 2898 2898 Size: 7003 SfcI C'TryA_G Cuts at: 826 1045 2525 3188 3400 3569 3898 4070 4278 Size: 219 1480 663 212 169 329 172 208 Cuts at: 4278 4351 5486 6167 6358 826 Size: 73 1135 681 191 1471 Fragments arranged by size: 1480 1471 1135 681 663 329 219 212 208 191 172 169 73 SfoI GGC'GCC Cuts at: 3925 3925 Size: 7003 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7003 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7003 SmaI CCC'GGG Cuts at: 1872 3968 4330 1872 Size: 2096 362 4545 Fragments arranged by size: 4545 2096 362 SmlI C'TyrA_G Cuts at: 820 1017 1943 5107 6008 6270 820 Size: 197 926 3164 901 262 1553 Fragments arranged by size: 3164 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4794 493 Size: 4301 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7003 SphI G_CATG'C Cuts at: 1132 4361 1132 Size: 3229 3774 SrfI GCCC'GGGC Cuts at: 3968 3968 Size: 7003 SspI AAT'ATT Cuts at: 5 52 4927 5 Size: 47 4875 2081 Fragments arranged by size: 4875 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7003 StyI C'CwwG_G Cuts at: 513 1065 2295 2886 2991 3468 3960 4060 4442 Size: 552 1230 591 105 477 492 100 382 Cuts at: 4442 513 Size: 3074 Fragments arranged by size: 3074 1230 591 552 492 477 382 105 100 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2450 3575 4187 5030 5047 5200 5385 Size: 1255 870 1125 612 843 17 153 185 Cuts at: 5385 5830 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1125 870 843 612 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3048 3198 3234 Size: 274 80 33 51 1443 1071 150 36 Cuts at: 3234 3732 5249 6961 96 Size: 498 1517 1712 138 Fragments arranged by size: 1712 1517 1443 1071 498 274 150 138 80 51 36 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6963 6995 6963 Size: 32 6971 Tth111I GACn'n_nGTC Cuts at: 1316 2900 1316 Size: 1584 5419 XbaI T'CTAG_A Cuts at: 4339 4339 Size: 7003 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2554 3022 3935 4018 2203 Size: 351 468 913 83 5188 Fragments arranged by size: 5188 913 468 351 83 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7003 XmaI C'CCGG_G Cuts at: 1870 3966 4328 1870 Size: 2096 362 4545 Fragments arranged by size: 4545 2096 362 XmnI GAAnn'nnTTC Cuts at: 3440 5132 3440 Size: 1692 5311 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4811 276 Size: 53 83 186 936 3277 2468 Fragments arranged by size: 3277 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SwaI