Restriction map for
pFN21AA1299
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# NO CUT ENZYMES ( 106 enzymes) #
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Acc65I AccII AclI AcsI AflII
AgeI AlwI AocI ApoI AscI
AseI AsnI Asp718I AviII AvrII
BalI BamHI BbrPI BclI BfrI
BglII BlnI BsaAI BsaBI BsiEI
BsiWI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstEII BstPI BstUI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
DrdI EagI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FspI HgaI HindIII HpaI KpnI
KspI MamI MfeI MflI MluI
MluNI MscI MunI MvnI NcoI
NheI NotI NruI NsiI PacI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tsp509I Van91I XbaI XcmI
XhoII
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# ONE CUT ENZYMES ( 55 enzymes) #
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AatII 982 GACGTC GACGT^C
AccI 1250 GTCTAC GT^[AC][GT]AC
AfaI 928 GTAC GT^AC
AflIII 1025 ACACGT A^C[GA][CT]GT
AhdI 1294 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1816 GTGCAC G^TGCAC
Aor51HI 452 AGCGCT AGC^GCT
ApaLI 1816 GTGCAC G^TGCAC
Asp700I 1396 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1294 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 712 GGCGCC GGCGC^C
BbsI 1651 GTCTTC GAAGAC(2/6)
BbvCI 772 GCTGAGG CCTCAGC(-5/-2)
BciVI 1572 GTATCC GTATCC(6/5)
BfaI 71 CTAG C^TAG
BlpI 205 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 205 GCTGAGC GC^T[ACGT]AGC
BpuAI 1651 GTCTTC GAAGAC(2/6)
BsaI 556 GAGACC GGTCTC(1/5)
BsmBI 41 CGTCTC CGTCTC(1/5)
BsmI 1117 GAATGC GAATGC(1/-1)
BsrFI 388 GCCGGC [GA]^CCGG[CT]
BstAPI 959 GCATCCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 881 TTCGAA TT^CGAA
CelII 205 GCTGAGC GC^T[ACGT]AGC
Cfr10I 388 GCCGGC [GA]^CCGG[CT]
DpnII 371 GATC ^GATC
EaeI 386 TGGCCG [CT]^GGCC[GA]
Eam1105I 1294 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 452 AGCGCT AGC^GCT
FseI 387 GGCCGGCC GGCCGG^CC
HincII 526 GTTGAC GT[CT]^[GA]AC
HindII 526 GTTGAC GT[CT]^[GA]AC
KasI 712 GGCGCC G^GCGCC
MaeI 71 CTAG C^TAG
MboI 371 GATC ^GATC
MseI 1037 TTAA T^TAA
NaeI 388 GCCGGC GCC^GGC
NarI 712 GGCGCC GG^CGCC
NdeI 1313 CATATG CA^TATG
NdeII 371 GATC ^GATC
NgoMIV 388 GCCGGC G^CCGGC
NspI 1086 GCATGT [GA]CATG^[CT]
NspV 881 TTCGAA TT^CGAA
PaeR7I 1801 CTCGAG C^TCGAG
RsaI 928 GTAC GT^AC
SacI 208 GAGCTC GAGCT^C
Sau3AI 371 GATC ^GATC
SfoI 712 GGCGCC GGC^GCC
SfuI 881 TTCGAA TT^CGAA
TfiI 1236 GAATC G^A[AT]TC
Tru9I 1037 TTAA T^TAA
Tsp45I 1988 GTGAC ^GT[GC]AC
XhoI 1801 CTCGAG C^TCGAG
XmnI 1396 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccIII 986 TCCGGA T^CCGGA
1497 TCCGGA
AcyI 712 GGCGCC G[GA]^CG[CT]C
982 GACGTC
AspI 558 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
695 GACTCAGTC
BanI 7 GGTGCC G^G[CT][GA]CC
712 GGCGCC
BcgI 1391 CGATGGAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
861 GCAGAAGTGTCG
BglI 232 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
852 GCCTCAGTGGC
BsaHI 712 GGCGCC G[GA]^CG[CT]C
982 GACGTC
BsaWI 986 TCCGGA [AT]^CCGG[AT]
1497 TCCGGA
BseAI 986 TCCGGA T^CCGGA
1497 TCCGGA
BspEI 986 TCCGGA T^CCGGA
1497 TCCGGA
BspMI 1265 ACCTGC ACCTGC(4/8)
1727 ACCTGC
BsrDI 1368 CATTGC GCAATG(2/0)
1289 GCAATG
BstXI 995 CCACAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1823 CCATCCCTTTGG
HaeII 452 AGCGCT [GA]GCGC^[CT]
712 GGCGCC
Hin1I 712 GGCGCC G[GA]^CG[CT]C
982 GACGTC
MaeIII 1988 GTGAC ^GT[ACGT]AC
1996 GTAAC
MroI 986 TCCGGA T^CCGGA
1497 TCCGGA
MspA1I 49 CCGCTG C[AC]G^C[GT]G
542 CCGCTG
PflFI 558 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
695 GACTCAGTC
StuI 938 AGGCCT AGG^CCT
1094 AGGCCT
Tth111I 558 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
695 GACTCAGTC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
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AciI AluI AlwNI ApaI AspHI
AvaI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DraII DsaI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MboII MnlI MslI MspI MvaI
MwoI NciI NlaIII NlaIV PleI
PpuMI PstI SapI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TseI TspRI TthHB8I XmaCI
XmaI