(Circular) (Six-base) MAPSORT of: pFN21AA1299.seq Check: 1639 from: 1 to: 6718 pFN21AA1299 6718 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3741 3741 Size: 6718 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2991 4528 278 Size: 53 83 186 936 1455 1537 2468 Fragments arranged by size: 2468 1537 1455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3256 4061 1937 Size: 1319 805 4594 Fragments arranged by size: 4594 1319 805 Acc65I G'GTAC_C Cuts at: 4039 4039 Size: 6718 AclI AA'CG_TT Cuts at: 1563 4845 5218 1563 Size: 3282 373 3063 Fragments arranged by size: 3282 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2823 4781 6165 6433 1632 Size: 1191 1958 1384 268 1917 Fragments arranged by size: 1958 1917 1384 1191 268 AfeI AGC'GCT Cuts at: 2459 2459 Size: 6718 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6521 AflIII A'CryG_T Cuts at: 1203 3030 5617 6594 1203 Size: 1827 2587 977 1327 Fragments arranged by size: 2587 1827 1327 977 AgeI A'CCGG_T Cuts at: 5549 5549 Size: 6718 AhdI GACnn_n'nnGTC Cuts at: 3304 5447 3304 Size: 2143 4575 AleI CACnn'nnGTG Cuts at: 1166 3035 1166 Size: 1869 4849 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3992 1184 Size: 2808 3910 AlwNI CAG_nnn'CTG Cuts at: 2792 3271 3778 4461 6033 2792 Size: 479 507 683 1572 3477 Fragments arranged by size: 3477 1572 683 507 479 ApaI G_GGCC'C Cuts at: 2178 2352 2369 2556 2178 Size: 174 17 187 6340 Fragments arranged by size: 6340 187 174 17 ApaLI G'TGCA_C Cuts at: 3821 4775 5931 3821 Size: 954 1156 4608 Fragments arranged by size: 4608 1156 954 ApoI r'AATT_y Cuts at: 1493 4027 4253 4405 6514 1493 Size: 2534 226 152 2109 1697 Fragments arranged by size: 2534 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5272 160 Size: 872 4240 1606 Fragments arranged by size: 4240 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6718 AvaI C'yCGr_G Cuts at: 1870 1943 2106 2177 2247 3806 4043 1870 Size: 73 163 71 70 1559 237 4545 Fragments arranged by size: 4545 1559 237 163 73 71 70 BamHI G'GATC_C Cuts at: 4048 4415 4048 Size: 367 6351 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2717 4039 5494 618 Size: 325 217 627 225 705 1322 1455 1842 Fragments arranged by size: 1842 1455 1322 705 627 325 225 217 BanII G_rGCy'C Cuts at: 1391 2178 2217 2294 2352 2369 2556 3134 4037 Size: 787 39 77 58 17 187 578 903 Cuts at: 4037 1391 Size: 4072 Fragments arranged by size: 4072 903 787 578 187 77 58 39 17 BbeI G_GCGC'C Cuts at: 2721 2721 Size: 6718 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3649 928 Size: 971 1750 3997 Fragments arranged by size: 3997 1750 971 BbvCI CC'TCA_GC Cuts at: 2778 2778 Size: 6718 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2855 2889 3385 3419 4909 4943 1903 Size: 34 918 34 496 34 1490 34 3678 Fragments arranged by size: 3678 1490 918 496 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3588 4610 5820 3588 Size: 1022 1210 4486 Fragments arranged by size: 4486 1210 1022 BclI T'GATC_A Cuts at: 1548 1548 Size: 6718 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2243 2863 5329 Size: 107 122 71 631 605 571 620 2466 Cuts at: 5329 136 Size: 1525 Fragments arranged by size: 2466 1525 631 620 605 571 122 107 71 BglII A'GATC_T Cuts at: 6713 6713 Size: 6718 BlpI GC'TnA_GC Cuts at: 2211 4135 2211 Size: 1924 4794 Bme1580I G_kGCm'C Cuts at: 1790 2017 2178 2352 2369 2556 3825 4779 5935 Size: 227 161 174 17 187 1269 954 1156 Cuts at: 5935 1790 Size: 2573 Fragments arranged by size: 2573 1269 1156 954 227 187 174 161 17 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6718 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2573 4010 4449 Size: 416 53 83 399 24 1156 1437 439 Cuts at: 4449 5407 442 Size: 958 1753 Fragments arranged by size: 1753 1437 1156 958 439 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 2942 3029 3245 3835 5378 1239 Size: 1340 363 87 216 590 1543 2579 Fragments arranged by size: 2579 1543 1340 590 363 216 87 Bpu10I CC'TnA_GC Cuts at: 2288 2778 3128 2288 Size: 490 350 5878 Fragments arranged by size: 5878 490 350 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2348 2435 3593 4843 5708 6006 2348 Size: 87 1158 1250 865 298 3060 Fragments arranged by size: 3060 1250 1158 865 298 87 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2555 5381 882 Size: 617 1056 2826 2219 Fragments arranged by size: 2826 2219 1056 617 BsaAI yAC'GTr Cuts at: 493 1204 4509 493 Size: 711 3305 2702 Fragments arranged by size: 3305 2702 711 BsaBI GATnn'nnATC Cuts at: 6712 6712 Size: 6718 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2718 2988 4525 4907 Size: 53 83 186 936 1185 270 1537 382 Cuts at: 4907 5542 275 Size: 635 1451 Fragments arranged by size: 1537 1451 1185 936 635 382 270 186 83 53 BsaWI w'CCGG_w Cuts at: 2991 3502 5151 5549 5823 5970 6381 2991 Size: 511 1649 398 274 147 411 3328 Fragments arranged by size: 3328 1649 511 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2021 2051 2138 2168 2525 Size: 30 165 30 618 30 87 30 357 Cuts at: 2525 2552 2555 2582 2585 2591 2615 2621 2808 Size: 27 3 27 3 6 24 6 187 Cuts at: 2808 2838 3017 3047 3746 3776 1178 Size: 30 179 30 699 30 4120 Fragments arranged by size: 4120 699 618 357 187 179 165 87 30 30 30 30 30 30 30 27 27 24 6 6 3 3 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2413 2425 2533 2599 2795 2832 2862 2919 Size: 1227 12 108 66 196 37 30 57 Cuts at: 2919 2922 3767 1186 Size: 3 845 4137 Fragments arranged by size: 4137 1227 845 196 108 66 57 37 30 12 3 BseYI C'CCAG_C Cuts at: 2362 2549 2621 2797 2828 3087 3260 3284 3359 Size: 187 72 176 31 259 173 24 75 Cuts at: 3359 3888 3914 5921 6417 2362 Size: 529 26 2007 496 2663 Fragments arranged by size: 2663 2007 529 496 259 187 176 173 75 72 31 26 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2765 3600 3952 1163 Size: 573 1029 835 352 3929 Fragments arranged by size: 3929 1029 835 573 352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4168 4929 5957 1540 Size: 275 186 2167 761 1028 2301 Fragments arranged by size: 2301 2167 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2217 3813 3825 4779 4864 5935 2217 Size: 1596 12 954 85 1071 3000 Fragments arranged by size: 3000 1596 1071 954 85 12 BsmI GAATG_Cn' Cuts at: 3128 4229 4322 3128 Size: 1101 93 5524 Fragments arranged by size: 5524 1101 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2052 2052 Size: 6718 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2017 2178 2217 2294 2352 2369 2556 Size: 399 227 161 39 77 58 17 187 Cuts at: 2556 3134 3813 3825 4037 4779 4864 5935 1391 Size: 578 679 12 212 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 679 578 399 227 212 187 161 85 77 58 39 17 12 BspEI T'CCGG_A Cuts at: 2991 3502 6381 2991 Size: 511 2879 3328 Fragments arranged by size: 3328 2879 511 BspHI T'CATG_A Cuts at: 4607 6337 4607 Size: 1730 4988 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3279 3741 4073 844 Size: 2435 462 332 3489 Fragments arranged by size: 3489 2435 462 332 BsrBI CCG'CTC Cuts at: 1394 1823 4605 1394 Size: 429 2782 3507 Fragments arranged by size: 3507 2782 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3301 3372 5213 5387 65 Size: 1183 2053 71 1841 174 1396 Fragments arranged by size: 2053 1841 1396 1183 174 71 BsrFI r'CCGG_y Cuts at: 1700 1810 2393 5362 5549 1700 Size: 110 583 2969 187 2869 Fragments arranged by size: 2969 2869 583 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6718 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6718 BssSI C'ACGA_G Cuts at: 4778 5790 4778 Size: 1012 5706 BstAPI GCAn_nnn'nTGC Cuts at: 794 2970 794 Size: 2176 4542 BstBI TT'CG_AA Cuts at: 2887 5522 2887 Size: 2635 4083 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6718 BstXI CCAn_nnnn'nTGG Cuts at: 3007 3835 3007 Size: 828 5890 BstYI r'GATC_y Cuts at: 1972 4048 4415 4800 4817 6269 6355 6367 6713 Size: 2076 367 385 17 1452 86 12 346 Cuts at: 6713 1972 Size: 1977 Fragments arranged by size: 2076 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2503 3589 3649 513 Size: 552 1438 1086 60 3582 Fragments arranged by size: 3582 1438 1086 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5979 Fragments arranged by size: 5979 683 56 BtsI GCAGTG_nn' Cuts at: 2522 4234 5028 5048 2522 Size: 1712 794 20 4192 Fragments arranged by size: 4192 1712 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6718 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6683 DraI TTT'AAA Cuts at: 4022 4022 Size: 6718 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5725 809 Size: 507 4409 1802 Fragments arranged by size: 4409 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2391 4165 5054 Size: 54 1425 50 275 18 561 1774 889 Cuts at: 5054 6683 8 Size: 1629 43 Fragments arranged by size: 1774 1629 1425 889 561 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4165 1537 Size: 275 2353 4090 Fragments arranged by size: 4090 2353 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2222 2422 2748 2980 3435 4648 1353 Size: 869 200 326 232 455 1213 3423 Fragments arranged by size: 3423 1213 869 455 326 232 200 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5305 5679 5825 387 Size: 1064 138 3716 374 146 1280 Fragments arranged by size: 3716 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2215 2215 Size: 6718 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2579 2823 2942 3029 3245 3835 4781 Size: 393 947 244 119 87 216 590 946 Cuts at: 4781 5378 6165 6433 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 947 946 787 597 590 393 268 244 216 119 87 EcoNI CCTnn'n_nnAGG Cuts at: 2838 3308 3530 2838 Size: 470 222 6026 Fragments arranged by size: 6026 470 222 EcoO109I rG'GnC_Cy Cuts at: 2034 2174 2349 2366 2386 2553 2573 3137 3189 Size: 140 175 17 20 167 20 564 52 Cuts at: 3189 3268 3325 3446 3519 2034 Size: 79 57 121 73 5233 Fragments arranged by size: 5233 564 175 167 140 121 79 73 57 52 20 20 17 EcoRI G'AATT_C Cuts at: 4027 4405 4027 Size: 378 6340 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2476 2476 Size: 6718 FseI GG_CCGG'CC Cuts at: 2397 2397 Size: 6718 FspI TGC'GCA Cuts at: 5224 5224 Size: 6718 HaeII r_GCGC'y Cuts at: 1678 2461 2721 5865 1678 Size: 783 260 3144 2531 Fragments arranged by size: 3144 2531 783 260 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2021 2053 2484 2516 2525 Size: 32 135 32 449 32 431 32 9 Cuts at: 2525 2552 2557 2584 2642 2674 2689 2721 2949 Size: 27 5 27 58 32 15 32 228 Cuts at: 2949 2981 3160 3192 3290 3322 3326 3358 3451 Size: 32 179 32 98 32 4 32 93 Cuts at: 3451 3483 3586 3618 5359 5391 5433 5465 1373 Size: 32 103 32 1741 32 42 32 2626 Fragments arranged by size: 2626 1741 449 431 228 179 135 103 98 93 58 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 27 27 15 9 5 4 HincII GTy'rAC Cuts at: 669 1657 1938 2533 4062 4308 669 Size: 988 281 595 1529 246 3079 Fragments arranged by size: 3079 1529 988 595 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6718 HpaI GTT'AAC Cuts at: 4308 4308 Size: 6718 KasI G'GCGC_C Cuts at: 2717 2717 Size: 6718 KpnI G_GTAC'C Cuts at: 4043 4043 Size: 6718 MfeI C'AATT_G Cuts at: 4317 4317 Size: 6718 MluI A'CGCG_T Cuts at: 6594 6594 Size: 6718 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2628 5832 6016 1305 Size: 1323 3204 184 2007 Fragments arranged by size: 3204 2007 1323 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4896 Fragments arranged by size: 4896 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3035 3957 3974 4676 5035 5194 518 Size: 648 1869 922 17 702 359 159 2042 Fragments arranged by size: 2042 1869 922 702 648 359 159 17 MspA1I CmG'CkG Cuts at: 1606 2056 2549 4134 4811 5959 6204 1606 Size: 450 493 1585 677 1148 245 2120 Fragments arranged by size: 2120 1585 1148 677 493 450 245 NaeI GCC'GGC Cuts at: 2395 2395 Size: 6718 NarI GG'CG_CC Cuts at: 2718 2718 Size: 6718 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6166 NdeI CA'TA_TG Cuts at: 387 3319 387 Size: 2932 3786 NgoMIV G'CCGG_C Cuts at: 2393 2393 Size: 6718 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6718 NotI GC'GGCC_GC Cuts at: 4165 4165 Size: 6718 NspI r_CATG'y Cuts at: 1132 1229 3095 4076 5621 1132 Size: 97 1866 981 1545 2229 Fragments arranged by size: 2229 1866 1545 981 97 PasI CC'CwG_GG Cuts at: 1348 2663 3185 1348 Size: 1315 522 4881 Fragments arranged by size: 4881 1315 522 PciI A'CATG_T Cuts at: 5617 5617 Size: 6718 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6718 PmeI GTTT'AAAC Cuts at: 4022 4022 Size: 6718 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4787 4819 6300 6332 4787 Size: 32 1481 32 5173 Fragments arranged by size: 5173 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2386 3137 3189 3268 3446 3519 2386 Size: 751 52 79 178 73 5585 Fragments arranged by size: 5585 751 178 79 73 52 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6718 PsiI TTA'TAA Cuts at: 4288 4288 Size: 6718 PspOMI G'GGCC_C Cuts at: 2174 2348 2365 2552 2174 Size: 174 17 187 6340 Fragments arranged by size: 6340 187 174 17 PspXI vC'TCGA_Gb Cuts at: 3806 3806 Size: 6718 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3569 3601 3569 Size: 32 6686 PstI C_TGCA'G Cuts at: 830 2226 2339 2517 4070 830 Size: 1396 113 178 1553 3478 Fragments arranged by size: 3478 1553 1396 178 113 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6718 SacI G_AGCT'C Cuts at: 2217 2217 Size: 6718 SalI G'TCGA_C Cuts at: 1936 4060 1936 Size: 2124 4594 SanDI GG'GwC_CC Cuts at: 3446 3446 Size: 6718 SapI GCTCTTCn'nnn_ Cuts at: 2222 2748 2980 2222 Size: 526 232 5960 Fragments arranged by size: 5960 526 232 SbfI CC_TGCA'GG Cuts at: 4070 4070 Size: 6718 ScaI AGT'ACT Cuts at: 4966 6678 4966 Size: 1712 5006 SfcI C'TryA_G Cuts at: 826 1045 2222 2335 2513 4066 5201 5882 6073 Size: 219 1177 113 178 1553 1135 681 191 Cuts at: 6073 826 Size: 1471 Fragments arranged by size: 1553 1471 1177 1135 681 219 191 178 113 SfoI GGC'GCC Cuts at: 2719 2719 Size: 6718 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6718 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6718 SmaI CCC'GGG Cuts at: 1872 2108 2179 2249 4045 1872 Size: 236 71 70 1796 4545 Fragments arranged by size: 4545 1796 236 71 70 SmlI C'TyrA_G Cuts at: 820 1017 1943 2327 2450 3608 3806 4822 5723 Size: 197 926 384 123 1158 198 1016 901 Cuts at: 5723 5985 820 Size: 262 1553 Fragments arranged by size: 1553 1158 1016 926 901 384 262 198 197 123 SnaBI TAC'GTA Cuts at: 493 4509 493 Size: 4016 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6718 SphI G_CATG'C Cuts at: 1132 4076 1132 Size: 2944 3774 SrfI GCCC'GGGC Cuts at: 2108 2249 2108 Size: 141 6577 SspI AAT'ATT Cuts at: 5 52 4642 5 Size: 47 4590 2081 Fragments arranged by size: 4590 2081 47 StuI AGG'CCT Cuts at: 1517 2945 3101 1517 Size: 1428 156 5134 Fragments arranged by size: 5134 1428 156 StyI C'CwwG_G Cuts at: 513 1065 2940 3492 3581 4157 513 Size: 552 1875 552 89 576 3074 Fragments arranged by size: 3074 1875 576 552 552 89 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2287 2333 2953 3094 4745 4762 4915 Size: 1255 707 46 620 141 1651 17 153 Cuts at: 4915 5100 5545 325 Size: 185 445 1498 Fragments arranged by size: 1651 1498 1255 707 620 445 185 153 141 46 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4964 6676 96 Size: 274 80 33 51 1443 2987 1712 138 Fragments arranged by size: 2987 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2808 2840 3746 3778 6678 6710 2808 Size: 32 906 32 2900 32 2816 Fragments arranged by size: 2900 2816 906 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2566 2703 1316 Size: 1250 137 5331 Fragments arranged by size: 5331 1250 137 XbaI T'CTAG_A Cuts at: 4054 4054 Size: 6718 XhoI C'TCGA_G Cuts at: 1943 3806 1943 Size: 1863 4855 XmaI C'CCGG_G Cuts at: 1870 2106 2177 2247 4043 1870 Size: 236 71 70 1796 4545 Fragments arranged by size: 4545 1796 236 71 70 XmnI GAAnn'nnTTC Cuts at: 3405 4847 3405 Size: 1442 5276 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2989 4526 276 Size: 53 83 186 936 1455 1537 2468 Fragments arranged by size: 2468 1537 1455 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoRV FspAI NruI NsiI PacI PfoI PmlI PvuII RsrII SacII SexAI SfiI SwaI XcmI