Restriction map for
pFN21AA1299
(Linear) (Six-base) MAPSORT of: KIAA1299.seq Check: 2722 from: 1 to: 2013
KIAA1299.orf
With 182 enzymes: SgfI *
February 28, 2007 11:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1736 2013
Size: 1736 277
AatII G_ACGT'C
Cuts at: 0 986 2013
Size: 986 1027
AccI GT'mk_AC
Cuts at: 0 1251 2013
Size: 1251 762
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 818 2013
Size: 818 1195
AfeI AGC'GCT
Cuts at: 0 454 2013
Size: 454 1559
AflIII A'CryG_T
Cuts at: 0 1025 2013
Size: 1025 988
AhdI GACnn_n'nnGTC
Cuts at: 0 1299 2013
Size: 1299 714
AleI CACnn'nnGTG
Cuts at: 0 1030 2013
Size: 1030 983
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1987 2013
Size: 1987 26
AlwNI CAG_nnn'CTG
Cuts at: 0 787 1266 1773 2013
Size: 787 479 507 240
Fragments arranged by size:
787 507 479 240
ApaI G_GGCC'C
Cuts at: 0 173 347 364 551 2013
Size: 173 174 17 187 1462
Fragments arranged by size:
1462 187 174 173 17
ApaLI G'TGCA_C
Cuts at: 0 1816 2013
Size: 1816 197
AvaI C'yCGr_G
Cuts at: 0 101 172 242 1801 2013
Size: 101 71 70 1559 212
Fragments arranged by size:
1559 212 101 71 70
BanI G'GyrC_C
Cuts at: 0 7 712 2013
Size: 7 705 1301
Fragments arranged by size:
1301 705 7
BanII G_rGCy'C
Cuts at: 0 173 212 289 347 364 551 1129 2013
Size: 173 39 77 58 17 187 578 884
Fragments arranged by size:
884 578 187 173 77 58 39 17
BbeI G_GCGC'C
Cuts at: 0 716 2013
Size: 716 1297
BbsI GAAGACnn'nnnn_
Cuts at: 0 1644 2013
Size: 1644 369
BbvCI CC'TCA_GC
Cuts at: 0 773 2013
Size: 773 1240
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 850 884 1380 1414 2013
Size: 850 34 496 34 599
Fragments arranged by size:
850 599 496 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1583 2013
Size: 1583 430
BglI GCCn_nnn'nGGC
Cuts at: 0 238 858 2013
Size: 238 620 1155
Fragments arranged by size:
1155 620 238
BlpI GC'TnA_GC
Cuts at: 0 206 2013
Size: 206 1807
Bme1580I G_kGCm'C
Cuts at: 0 12 173 347 364 551 1820 2013
Size: 12 161 174 17 187 1269 193
Fragments arranged by size:
1269 193 187 174 161 17 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 568 2013
Size: 568 1445
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 937 1024 1240 1830 2013
Size: 574 363 87 216 590 183
Fragments arranged by size:
590 574 363 216 183 87
Bpu10I CC'TnA_GC
Cuts at: 0 283 773 1123 2013
Size: 283 490 350 890
Fragments arranged by size:
890 490 350 283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 343 430 1588 2013
Size: 343 87 1158 425
Fragments arranged by size:
1158 425 343 87
BsaI GGTCTCn'nnnn_
Cuts at: 0 550 2013
Size: 550 1463
BsaHI Gr'CG_yC
Cuts at: 0 713 983 2013
Size: 713 270 1030
Fragments arranged by size:
1030 713 270
BsaWI w'CCGG_w
Cuts at: 0 986 1497 2013
Size: 986 511 516
Fragments arranged by size:
986 516 511
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 16 46 133 163 520 547 550 577
Size: 16 30 87 30 357 27 3 27
Cuts at: 577 580 586 610 616 803 833 1012 1042
Size: 3 6 24 6 187 30 179 30
Cuts at: 1042 1741 1771 2013
Size: 699 30 242
Fragments arranged by size:
699 357 242 187 179 87 30 30
30 30 30 27 27 24 16 6
6 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 408 420 528 594 790 827 857 914
Size: 408 12 108 66 196 37 30 57
Cuts at: 914 917 1762 2013
Size: 3 845 251
Fragments arranged by size:
845 408 251 196 108 66 57 37
30 12 3
BseYI C'CCAG_C
Cuts at: 0 357 544 616 792 823 1082 1255 1279
Size: 357 187 72 176 31 259 173 24
Cuts at: 1279 1354 1883 1909 2013
Size: 75 529 26 104
Fragments arranged by size:
529 357 259 187 176 173 104 75
72 31 26 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1595 1947 2013
Size: 760 835 352 66
Fragments arranged by size:
835 760 352 66
BsiHKAI G_wGCw'C
Cuts at: 0 212 1808 1820 2013
Size: 212 1596 12 193
Fragments arranged by size:
1596 212 193 12
BsmI GAATG_Cn'
Cuts at: 0 1123 2013
Size: 1123 890
BsmBI CGTCTCn'nnnn_
Cuts at: 0 47 2013
Size: 47 1966
Bsp1286I G_dGCh'C
Cuts at: 0 12 173 212 289 347 364 551 1129
Size: 12 161 39 77 58 17 187 578
Cuts at: 1129 1808 1820 2013
Size: 679 12 193
Fragments arranged by size:
679 578 193 187 161 77 58 39
17 12 12
BspEI T'CCGG_A
Cuts at: 0 986 1497 2013
Size: 986 511 516
Fragments arranged by size:
986 516 511
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1274 1736 2013
Size: 1274 462 277
Fragments arranged by size:
1274 462 277
BsrDI GCAATG_nn'
Cuts at: 0 1296 1367 2013
Size: 1296 71 646
Fragments arranged by size:
1296 646 71
BsrFI r'CCGG_y
Cuts at: 0 388 2013
Size: 388 1625
BstAPI GCAn_nnn'nTGC
Cuts at: 0 965 2013
Size: 965 1048
BstBI TT'CG_AA
Cuts at: 0 882 2013
Size: 882 1131
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1002 1830 2013
Size: 1002 828 183
Fragments arranged by size:
1002 828 183
BtgI C'CryG_G
Cuts at: 0 498 1584 1644 2013
Size: 498 1086 60 369
Fragments arranged by size:
1086 498 369 60
BtsI GCAGTG_nn'
Cuts at: 0 517 2013
Size: 517 1496
EaeI y'GGCC_r
Cuts at: 0 386 2013
Size: 386 1627
EarI CTCTTCn'nnn_
Cuts at: 0 217 417 743 975 1430 2013
Size: 217 200 326 232 455 583
Fragments arranged by size:
583 455 326 232 217 200
EcoICRI GAG'CTC
Cuts at: 0 210 2013
Size: 210 1803
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 574 818 937 1024 1240 1830 2013
Size: 574 244 119 87 216 590 183
Fragments arranged by size:
590 574 244 216 183 119 87
EcoNI CCTnn'n_nnAGG
Cuts at: 0 833 1303 1525 2013
Size: 833 470 222 488
Fragments arranged by size:
833 488 470 222
EcoO109I rG'GnC_Cy
Cuts at: 0 29 169 344 361 381 548 568 1132
Size: 29 140 175 17 20 167 20 564
Cuts at: 1132 1184 1263 1320 1441 1514 2013
Size: 52 79 57 121 73 499
Fragments arranged by size:
564 499 175 167 140 121 79 73
57 52 29 20 20 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 471 2013
Size: 471 1542
FseI GG_CCGG'CC
Cuts at: 0 392 2013
Size: 392 1621
HaeII r_GCGC'y
Cuts at: 0 456 716 2013
Size: 456 260 1297
Fragments arranged by size:
1297 456 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 479 511 520 547 552 579
Size: 16 32 431 32 9 27 5 27
Cuts at: 579 637 669 684 716 944 976 1155 1187
Size: 58 32 15 32 228 32 179 32
Cuts at: 1187 1285 1317 1321 1353 1446 1478 1581 1613
Size: 98 32 4 32 93 32 103 32
Cuts at: 1613 2013
Size: 400
Fragments arranged by size:
431 400 228 179 103 98 93 58
32 32 32 32 32 32 32 32
32 32 27 27 16 15 9 5
4
HincII GTy'rAC
Cuts at: 0 528 2013
Size: 528 1485
KasI G'GCGC_C
Cuts at: 0 712 2013
Size: 712 1301
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 623 2013
Size: 623 1390
MslI CAynn'nnrTG
Cuts at: 0 1030 1952 1969 2013
Size: 1030 922 17 44
Fragments arranged by size:
1030 922 44 17
MspA1I CmG'CkG
Cuts at: 0 51 544 2013
Size: 51 493 1469
Fragments arranged by size:
1469 493 51
NaeI GCC'GGC
Cuts at: 0 390 2013
Size: 390 1623
NarI GG'CG_CC
Cuts at: 0 713 2013
Size: 713 1300
NdeI CA'TA_TG
Cuts at: 0 1314 2013
Size: 1314 699
NgoMIV G'CCGG_C
Cuts at: 0 388 2013
Size: 388 1625
NspI r_CATG'y
Cuts at: 0 1090 2013
Size: 1090 923
PasI CC'CwG_GG
Cuts at: 0 658 1180 2013
Size: 658 522 833
Fragments arranged by size:
833 658 522
PpuMI rG'GwC_Cy
Cuts at: 0 381 1132 1184 1263 1441 1514 2013
Size: 381 751 52 79 178 73 499
Fragments arranged by size:
751 499 381 178 79 73 52
PspOMI G'GGCC_C
Cuts at: 0 169 343 360 547 2013
Size: 169 174 17 187 1466
Fragments arranged by size:
1466 187 174 169 17
PspXI vC'TCGA_Gb
Cuts at: 0 1801 2013
Size: 1801 212
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1564 1596 2013
Size: 1564 32 417
Fragments arranged by size:
1564 417 32
PstI C_TGCA'G
Cuts at: 0 221 334 512 2013
Size: 221 113 178 1501
Fragments arranged by size:
1501 221 178 113
SacI G_AGCT'C
Cuts at: 0 212 2013
Size: 212 1801
SanDI GG'GwC_CC
Cuts at: 0 1441 2013
Size: 1441 572
SapI GCTCTTCn'nnn_
Cuts at: 0 217 743 975 2013
Size: 217 526 232 1038
Fragments arranged by size:
1038 526 232 217
SfcI C'TryA_G
Cuts at: 0 217 330 508 2013
Size: 217 113 178 1505
Fragments arranged by size:
1505 217 178 113
SfoI GGC'GCC
Cuts at: 0 714 2013
Size: 714 1299
SmaI CCC'GGG
Cuts at: 0 103 174 244 2013
Size: 103 71 70 1769
Fragments arranged by size:
1769 103 71 70
SmlI C'TyrA_G
Cuts at: 0 322 445 1603 1801 2013
Size: 322 123 1158 198 212
Fragments arranged by size:
1158 322 212 198 123
SrfI GCCC'GGGC
Cuts at: 0 103 244 2013
Size: 103 141 1769
Fragments arranged by size:
1769 141 103
StuI AGG'CCT
Cuts at: 0 940 1096 2013
Size: 940 156 917
Fragments arranged by size:
940 917 156
StyI C'CwwG_G
Cuts at: 0 935 1487 1576 2013
Size: 935 552 89 437
Fragments arranged by size:
935 552 437 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 282 328 948 1089 2013
Size: 282 46 620 141 924
Fragments arranged by size:
924 620 282 141 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 803 835 1741 1773 2013
Size: 803 32 906 32 240
Fragments arranged by size:
906 803 240 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 561 698 2013
Size: 561 137 1315
Fragments arranged by size:
1315 561 137
XhoI C'TCGA_G
Cuts at: 0 1801 2013
Size: 1801 212
XmaI C'CCGG_G
Cuts at: 0 101 172 242 2013
Size: 101 71 70 1771
Fragments arranged by size:
1771 101 71 70
XmnI GAAnn'nnTTC
Cuts at: 0 1400 2013
Size: 1400 613
ZraI GAC'GTC
Cuts at: 0 984 2013
Size: 984 1029
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrDI BsrFI BstAPI BstBI BstXI BtgI BtsI EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII
Hin4I HincII KasI MmeI MslI MspA1I NaeI NarI
NdeI NgoMIV NspI PasI PpuMI PspOMI PspXI PsrI
PstI SacI SanDI SapI SfcI SfoI SmaI SmlI
SrfI StuI StyI TaqII TstI Tth111I XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AloI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BclI BfrBI BglII BmgBI
BmtI BplI BsaAI BsaBI BsiEI BsiWI BspHI BsrBI
BsrGI BssHII BssSI BstEII BstYI BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoRI
EcoRV FspI FspAI HindIII HpaI KpnI MfeI MluI
MscI NcoI NheI NotI NruI NsiI PacI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PvuI
PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SspI SwaI TatI
XbaI XcmI