(Circular) (Six-base) MAPSORT of: pFN21AA1097.seq Check: 5485 from: 1 to: 7438 pFN21AA1097 7438 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5248 278 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3087 3173 4781 1937 Size: 1150 86 1608 4594 Fragments arranged by size: 4594 1608 1150 86 Acc65I G'GTAC_C Cuts at: 4759 4759 Size: 7438 AclI AA'CG_TT Cuts at: 1563 5565 5938 1563 Size: 4002 373 3063 Fragments arranged by size: 4002 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2952 3063 3120 3821 4017 4109 4626 4671 Size: 1320 111 57 701 196 92 517 45 Cuts at: 4671 5501 6885 7153 1632 Size: 830 1384 268 1917 Fragments arranged by size: 1917 1384 1320 830 701 517 268 196 111 92 57 45 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 6337 7314 1203 Size: 5134 977 1327 Fragments arranged by size: 5134 1327 977 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 3394 4319 6167 3394 Size: 925 1848 4665 Fragments arranged by size: 4665 1848 925 AleI CACnn'nnGTG Cuts at: 1166 3532 1166 Size: 2366 5072 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4481 1184 Size: 3297 4141 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4104 4136 4104 Size: 32 7406 AlwNI CAG_nnn'CTG Cuts at: 2651 3379 4592 5181 6753 2651 Size: 728 1213 589 1572 3336 Fragments arranged by size: 3336 1572 1213 728 589 ApaLI G'TGCA_C Cuts at: 5495 6651 5495 Size: 1156 6282 ApoI r'AATT_y Cuts at: 1493 2732 3037 3050 3974 4093 4157 4398 4618 Size: 1239 305 13 924 119 64 241 220 Cuts at: 4618 4747 4973 5125 7234 1493 Size: 129 226 152 2109 1697 Fragments arranged by size: 2109 1697 1239 924 305 241 226 220 152 129 119 64 13 AseI AT'TA_AT Cuts at: 160 1032 3027 5992 160 Size: 872 1995 2965 1606 Fragments arranged by size: 2965 1995 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 3407 4763 1870 Size: 73 1464 1356 4545 Fragments arranged by size: 4545 1464 1356 73 BamHI G'GATC_C Cuts at: 4768 5135 4768 Size: 367 7071 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3262 4759 6214 618 Size: 325 217 627 1475 1497 1455 1842 Fragments arranged by size: 1842 1497 1475 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4757 1391 Size: 3366 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2844 3456 4257 928 Size: 971 945 612 801 4109 Fragments arranged by size: 4109 971 945 801 612 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3503 3537 5629 5663 1903 Size: 34 1566 34 2092 34 3678 Fragments arranged by size: 3678 2092 1566 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5330 6540 5330 Size: 1210 6228 BclI T'GATC_A Cuts at: 1548 3982 1548 Size: 2434 5004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6049 136 Size: 107 122 71 631 605 4377 1525 Fragments arranged by size: 4377 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3421 7433 3421 Size: 4012 3426 BlpI GC'TnA_GC Cuts at: 3459 4855 3459 Size: 1396 6042 Bme1580I G_kGCm'C Cuts at: 1790 5499 6655 1790 Size: 3709 1156 2573 Fragments arranged by size: 3709 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7438 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2364 5169 6127 Size: 416 53 83 399 24 947 2805 958 Cuts at: 6127 442 Size: 1753 Fragments arranged by size: 2805 1753 958 947 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2156 4425 4503 6098 1239 Size: 917 2269 78 1595 2579 Fragments arranged by size: 2579 2269 1595 917 78 Bpu10I CC'TnA_GC Cuts at: 3852 4574 3852 Size: 722 6716 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2712 5563 6428 6726 2712 Size: 2851 865 298 3424 Fragments arranged by size: 3424 2851 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3404 6101 882 Size: 617 1905 2697 2219 Fragments arranged by size: 2697 2219 1905 617 BsaAI yAC'GTr Cuts at: 493 1204 5229 493 Size: 711 4025 2702 Fragments arranged by size: 4025 2702 711 BsaBI GATnn'nnATC Cuts at: 4512 7432 4512 Size: 2920 4518 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5245 5627 6262 275 Size: 53 83 186 936 3712 382 635 1451 Fragments arranged by size: 3712 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4669 5871 6269 6543 6690 7101 4669 Size: 1202 398 274 147 411 5006 Fragments arranged by size: 5006 1202 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4104 4134 1178 Size: 30 165 30 2701 30 4482 Fragments arranged by size: 4482 2701 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2382 2837 4093 1186 Size: 1196 455 1256 4531 Fragments arranged by size: 4531 1256 1196 455 BseYI C'CCAG_C Cuts at: 6641 7137 6641 Size: 496 6942 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3392 1163 Size: 573 1656 5209 Fragments arranged by size: 5209 1656 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4888 5649 6677 1540 Size: 275 186 2887 761 1028 2301 Fragments arranged by size: 2887 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5499 5584 6655 5499 Size: 85 1071 6282 Fragments arranged by size: 6282 1071 85 BsmI GAATG_Cn' Cuts at: 2506 3466 4949 5042 2506 Size: 960 1483 93 4902 Fragments arranged by size: 4902 1483 960 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4757 5499 5584 6655 1391 Size: 399 2967 742 85 1071 2174 Fragments arranged by size: 2967 2174 1071 742 399 85 BspEI T'CCGG_A Cuts at: 7101 7101 Size: 7438 BspHI T'CATG_A Cuts at: 2761 5327 7057 2761 Size: 2566 1730 3142 Fragments arranged by size: 3142 2566 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2596 4290 4793 844 Size: 1752 1694 503 3489 Fragments arranged by size: 3489 1752 1694 503 BsrBI CCG'CTC Cuts at: 1394 1823 5325 1394 Size: 429 3502 3507 Fragments arranged by size: 3507 3502 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2306 5933 6107 65 Size: 1183 1058 3627 174 1396 Fragments arranged by size: 3627 1396 1183 1058 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6082 6269 1700 Size: 110 4272 187 2869 Fragments arranged by size: 4272 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3672 4015 4332 96 Size: 3576 343 317 3202 Fragments arranged by size: 3576 3202 343 317 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7438 BssSI C'ACGA_G Cuts at: 5498 6510 5498 Size: 1012 6426 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7438 BstBI TT'CG_AA Cuts at: 2017 3161 6242 2017 Size: 1144 3081 3213 Fragments arranged by size: 3213 3081 1144 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7438 BstXI CCAn_nnnn'nTGG Cuts at: 2124 3534 2124 Size: 1410 6028 BstYI r'GATC_y Cuts at: 1972 2648 2800 3183 3421 3832 4513 4768 5135 Size: 676 152 383 238 411 681 255 367 Cuts at: 5135 5520 5537 6989 7075 7087 7433 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 681 676 411 385 383 367 346 255 238 152 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6886 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6699 Fragments arranged by size: 6699 683 56 BtsI GCAGTG_nn' Cuts at: 3380 4475 4954 5748 5768 3380 Size: 1095 479 794 20 5050 Fragments arranged by size: 5050 1095 794 479 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7438 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7403 DraI TTT'AAA Cuts at: 2362 4742 2362 Size: 2380 5058 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6445 809 Size: 507 5129 1802 Fragments arranged by size: 5129 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3251 4594 4885 Size: 54 1425 50 275 18 1421 1343 291 Cuts at: 4885 5774 7403 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1421 1343 889 291 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4885 1537 Size: 275 3073 4090 Fragments arranged by size: 4090 3073 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2244 3557 4046 4056 5368 1353 Size: 891 1313 489 10 1312 3423 Fragments arranged by size: 3423 1313 1312 891 489 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4376 6025 6399 6545 387 Size: 1064 138 2787 1649 374 146 1280 Fragments arranged by size: 2787 1649 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2156 2952 3063 3120 3821 4017 4109 Size: 393 524 796 111 57 701 196 92 Cuts at: 4109 4425 4503 4626 4671 5501 6098 6885 7153 Size: 316 78 123 45 830 597 787 268 Cuts at: 7153 1239 Size: 1524 Fragments arranged by size: 1524 830 796 787 701 597 524 393 316 268 196 123 111 92 78 57 45 EcoNI CCTnn'n_nnAGG Cuts at: 3237 3237 Size: 7438 EcoO109I rG'GnC_Cy Cuts at: 4523 4646 4523 Size: 123 7315 EcoRI G'AATT_C Cuts at: 2732 4747 5125 2732 Size: 2015 378 5045 Fragments arranged by size: 5045 2015 378 EcoRV GAT'ATC Cuts at: 4089 4089 Size: 7438 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2462 2753 3644 2462 Size: 291 891 6256 Fragments arranged by size: 6256 891 291 FspI TGC'GCA Cuts at: 5944 5944 Size: 7438 HaeII r_GCGC'y Cuts at: 1678 6585 1678 Size: 4907 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2852 2884 3053 3059 3085 Size: 32 135 32 1280 32 169 6 26 Cuts at: 3085 3091 3380 3412 3868 3875 3900 3907 4255 Size: 6 289 32 456 7 25 7 348 Cuts at: 4255 4287 4305 4337 6079 6111 6153 6185 1373 Size: 32 18 32 1742 32 42 32 2626 Fragments arranged by size: 2626 1742 1280 456 348 289 169 135 42 32 32 32 32 32 32 32 32 26 25 18 7 7 6 6 HincII GTy'rAC Cuts at: 669 1657 1938 3088 4324 4782 5028 669 Size: 988 281 1150 1236 458 246 3079 Fragments arranged by size: 3079 1236 1150 988 458 281 246 HindIII A'AGCT_T Cuts at: 748 4029 748 Size: 3281 4157 HpaI GTT'AAC Cuts at: 5028 5028 Size: 7438 KpnI G_GTAC'C Cuts at: 4763 4763 Size: 7438 MfeI C'AATT_G Cuts at: 5037 5037 Size: 7438 MluI A'CGCG_T Cuts at: 7314 7314 Size: 7438 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2720 2832 3506 6552 6736 1305 Size: 1415 112 674 3046 184 2007 Fragments arranged by size: 3046 2007 1415 674 184 112 MscI TGG'CCA Cuts at: 10 64 1489 1832 3253 4596 10 Size: 54 1425 343 1421 1343 2852 Fragments arranged by size: 2852 1425 1421 1343 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3162 3532 4218 4463 5396 5755 5914 Size: 648 1996 370 686 245 933 359 159 Cuts at: 5914 518 Size: 2042 Fragments arranged by size: 2042 1996 933 686 648 370 359 245 159 MspA1I CmG'CkG Cuts at: 1606 4319 4854 5531 6679 6924 1606 Size: 2713 535 677 1148 245 2120 Fragments arranged by size: 2713 2120 1148 677 535 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6886 NdeI CA'TA_TG Cuts at: 387 2152 3520 3880 387 Size: 1765 1368 360 3945 Fragments arranged by size: 3945 1765 1368 360 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7438 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NspI r_CATG'y Cuts at: 1132 1229 2133 4796 6341 1132 Size: 97 904 2663 1545 2229 Fragments arranged by size: 2663 2229 1545 904 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7438 PciI A'CATG_T Cuts at: 6337 6337 Size: 7438 PflMI CCAn_nnn'nTGG Cuts at: 1108 3440 1108 Size: 2332 5106 PfoI T'CCnGG_A Cuts at: 2352 2352 Size: 7438 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4104 4136 5507 5539 7020 7052 4104 Size: 32 1371 32 1481 32 4490 Fragments arranged by size: 4490 1481 1371 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4523 4523 Size: 7438 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7438 PsiI TTA'TAA Cuts at: 5008 5008 Size: 7438 PstI C_TGCA'G Cuts at: 830 4629 4790 830 Size: 3799 161 3478 Fragments arranged by size: 3799 3478 161 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 PvuII CAG'CTG Cuts at: 4319 4319 Size: 7438 SalI G'TCGA_C Cuts at: 1936 3086 4780 1936 Size: 1150 1694 4594 Fragments arranged by size: 4594 1694 1150 SapI GCTCTTCn'nnn_ Cuts at: 4046 4046 Size: 7438 SbfI CC_TGCA'GG Cuts at: 4790 4790 Size: 7438 ScaI AGT'ACT Cuts at: 5686 7398 5686 Size: 1712 5726 SexAI A'CCwGG_T Cuts at: 4254 4254 Size: 7438 SfcI C'TryA_G Cuts at: 826 1045 3489 4045 4625 4786 5921 6602 6793 Size: 219 2444 556 580 161 1135 681 191 Cuts at: 6793 826 Size: 1471 Fragments arranged by size: 2444 1471 1135 681 580 556 219 191 161 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 4765 1872 Size: 2893 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2727 3407 5542 6443 6705 820 Size: 197 926 784 680 2135 901 262 1553 Fragments arranged by size: 2135 1553 926 901 784 680 262 197 SnaBI TAC'GTA Cuts at: 493 5229 493 Size: 4736 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 4796 1132 Size: 3664 3774 SspI AAT'ATT Cuts at: 5 52 2479 5362 5 Size: 47 2427 2883 2081 Fragments arranged by size: 2883 2427 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7438 StyI C'CwwG_G Cuts at: 513 1065 2083 2110 4877 513 Size: 552 1018 27 2767 3074 Fragments arranged by size: 3074 2767 1018 552 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4719 5465 5482 5635 5820 6265 325 Size: 1255 3139 746 17 153 185 445 1498 Fragments arranged by size: 3139 1498 1255 746 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3672 3724 4015 Size: 274 80 33 51 1443 1695 52 291 Cuts at: 4015 4332 5684 7396 96 Size: 317 1352 1712 138 Fragments arranged by size: 1712 1695 1443 1352 317 291 274 138 80 52 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2640 2672 3168 3200 7398 7430 2640 Size: 32 496 32 4198 32 2648 Fragments arranged by size: 4198 2648 496 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7438 XbaI T'CTAG_A Cuts at: 2918 4774 2918 Size: 1856 5582 XhoI C'TCGA_G Cuts at: 1943 3407 1943 Size: 1464 5974 XmaI C'CCGG_G Cuts at: 1870 4763 1870 Size: 2893 4545 XmnI GAAnn'nnTTC Cuts at: 2736 5567 2736 Size: 2831 4607 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5246 276 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI XcmI