Restriction map for
pFN21AA1042
##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII AccI AccII AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp700I AspEI AvrII
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsiWI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstZ17I ClaI
CpoI DraI DraIII Eam1105I EcoRI
EcoRV EcoT22I FbaI HpaI KasI
KspI MamI MfeI MluI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfoI SfuI SnaBI SpeI Sse8387I
SspBI SspI SwaI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 2667 GGTACC G^GTACC
AcyI 1455 GACGCC G[GA]^CG[CT]C
AflIII 2504 ACGTGT A^C[GA][CT]GT
Alw44I 2400 GTGCAC G^TGCAC
Aor51HI 538 AGCGCT AGC^GCT
ApaI 2626 GGGCCC GGGCC^C
ApaLI 2400 GTGCAC G^TGCAC
Asp718I 2667 GGTACC G^GTACC
AspI 2533 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BglI 2628 GCCCCCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1246 AGATCT A^GATCT
BsaHI 1455 GACGCC G[GA]^CG[CT]C
BsgI 2302 CTGCAC GTGCAG(16/14)
BsiEI 2286 CGGCCG CG[GA][CT]^CG
EagI 2286 CGGCCG C^GGCCG
EclXI 2286 CGGCCG C^GGCCG
Eco47III 538 AGCGCT AGC^GCT
Eco52I 2286 CGGCCG C^GGCCG
FseI 2283 GGCCGGCC GGCCGG^CC
Hin1I 1455 GACGCC G[GA]^CG[CT]C
HincII 1849 GTTGAC GT[CT]^[GA]AC
HindII 1849 GTTGAC GT[CT]^[GA]AC
HindIII 896 AAGCTT A^AGCTT
KpnI 2667 GGTACC GGTAC^C
NaeI 2284 GCCGGC GCC^GGC
NgoMIV 2284 GCCGGC G^CCGGC
PflFI 2533 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 2610 GGGACCC [GA]G^G[AT]CC[CT]
SacI 2044 GAGCTC GAGCT^C
SexAI 1229 ACCAGGT A^CC[AT]GGT
SfiI 2627 GGCCCCCGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 2120 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2631 CCCGGG CCC^GGG
SmlI 355 CTTGAG C^T[CT][GA]AG
SphI 2780 GCATGC GCATG^C
StuI 2661 AGGCCT AGG^CCT
Tth111I 2533 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2631 CCCGGG C^CCGGG
XmaI 2631 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 2454 CCTGAGG CC^T[ACGT]AGG
2671 CCTGAGG
BanI 1462 GGCACC G^G[CT][GA]CC
2667 GGTACC
BbsI 702 GAAGAC GAAGAC(2/6)
816 GAAGAC
BlpI 37 GCTCAGC GC^T[ACGT]AGC
2830 GCTAAGC
Bpu1102I 37 GCTCAGC GC^T[ACGT]AGC
2830 GCTAAGC
BpuAI 702 GAAGAC GAAGAC(2/6)
816 GAAGAC
BsaI 709 GAGACC GGTCTC(1/5)
1954 GAGACC
BstEII 1821 GGTCACC G^GT[ACGT]ACC
1900 GGTGACC
BstPI 1821 GGTCACC G^GT[ACGT]ACC
1900 GGTGACC
BstYI 1246 AGATCT [GA]^GATC[CT]
2773 GGATCT
Bsu36I 2454 CCTGAGG CC^T[ACGT]AGG
2671 CCTGAGG
CelII 37 GCTCAGC GC^T[ACGT]AGC
2830 GCTAAGC
DrdI 2499 GACCGACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2503 GACGTGTCCGTC
Eco81I 2454 CCTGAGG CC^T[ACGT]AGG
2671 CCTGAGG
EcoO65I 1821 GGTCACC G^GT[ACGT]ACC
1900 GGTGACC
MflI 1246 AGATCT [GA]^GATC[CT]
2773 GGATCT
NcoI 1109 CCATGG C^CATGG
2765 CCATGG
PstI 1156 CTGCAG CTGCA^G
2722 CTGCAG
SfcI 1156 CTGCAG C^T[GA][CT]AG
2722 CTGCAG
XcmI 1109 CCATGGATTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1779 CCAACCTCACCTTGG
XhoII 1246 AGATCT [GA]^GATC[CT]
2773 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccIII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII AviII
BalI BanII BbvCI BbvI BcnI
BfaI BmyI BpmI BsaJI BsaWI
BseAI BseRI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstXI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI FspI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I