Restriction map for
pFN21AA1042
(Linear) (Six-base) MAPSORT of: KIAA1042.seq Check: 2381 from: 1 to: 2859
KIAA1042.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 447 866 2859
Size: 447 419 1993
Fragments arranged by size:
1993 447 419
Acc65I G'GTAC_C
Cuts at: 0 2667 2859
Size: 2667 192
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 721 1060 1674 2021 2859
Size: 508 213 339 614 347 838
Fragments arranged by size:
838 614 508 347 339 213
AfeI AGC'GCT
Cuts at: 0 540 2859
Size: 540 2319
AflIII A'CryG_T
Cuts at: 0 2504 2859
Size: 2504 355
AleI CACnn'nnGTG
Cuts at: 0 2122 2767 2859
Size: 2122 645 92
Fragments arranged by size:
2122 645 92
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 915 1030 1171 2859
Size: 915 115 141 1688
Fragments arranged by size:
1688 915 141 115
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1525 1557 2859
Size: 1525 32 1302
Fragments arranged by size:
1525 1302 32
AlwNI CAG_nnn'CTG
Cuts at: 0 45 741 933 1578 2859
Size: 45 696 192 645 1281
Fragments arranged by size:
1281 696 645 192 45
ApaI G_GGCC'C
Cuts at: 0 2630 2859
Size: 2630 229
ApaLI G'TGCA_C
Cuts at: 0 2400 2859
Size: 2400 459
AvaI C'yCGr_G
Cuts at: 0 559 2051 2631 2811 2859
Size: 559 1492 580 180 48
Fragments arranged by size:
1492 580 559 180 48
BanI G'GyrC_C
Cuts at: 0 1462 2667 2859
Size: 1462 1205 192
Fragments arranged by size:
1462 1205 192
BanII G_rGCy'C
Cuts at: 0 39 560 2048 2219 2296 2410 2583 2630
Size: 39 521 1488 171 77 114 173 47
Cuts at: 2630 2859
Size: 229
Fragments arranged by size:
1488 521 229 173 171 114 77 47
39
BbsI GAAGACnn'nnnn_
Cuts at: 0 709 823 2859
Size: 709 114 2036
Fragments arranged by size:
2036 709 114
BbvCI CC'TCA_GC
Cuts at: 0 766 1495 1959 2859
Size: 766 729 464 900
Fragments arranged by size:
900 766 729 464
BglI GCCn_nnn'nGGC
Cuts at: 0 2634 2859
Size: 2634 225
BglII A'GATC_T
Cuts at: 0 1246 2859
Size: 1246 1613
BlpI GC'TnA_GC
Cuts at: 0 38 2831 2859
Size: 38 2793 28
Fragments arranged by size:
2793 38 28
Bme1580I G_kGCm'C
Cuts at: 0 1465 1645 2404 2630 2859
Size: 1465 180 759 226 229
Fragments arranged by size:
1465 759 229 226 180
BmgBI CAC'GTC
Cuts at: 0 1076 2505 2859
Size: 1076 1429 354
Fragments arranged by size:
1429 1076 354
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 618 2859
Size: 618 2241
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 212 469 1006 1163 1504 1555 1705
Size: 160 52 257 537 157 341 51 150
Cuts at: 1705 1922 2069 2859
Size: 217 147 790
Fragments arranged by size:
790 537 341 257 217 160 157 150
147 52 51
Bpu10I CC'TnA_GC
Cuts at: 0 766 944 1495 1959 2859
Size: 766 178 551 464 900
Fragments arranged by size:
900 766 551 464 178
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 376 2859
Size: 376 2483
BsaI GGTCTCn'nnnn_
Cuts at: 0 703 1948 2859
Size: 703 1245 911
Fragments arranged by size:
1245 911 703
BsaHI Gr'CG_yC
Cuts at: 0 1456 2859
Size: 1456 1403
BsaWI w'CCGG_w
Cuts at: 0 114 1049 1835 2859
Size: 114 935 786 1024
Fragments arranged by size:
1024 935 786 114
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 466 496 979 1009 1144 1174 1245 1275
Size: 466 30 483 30 135 30 71 30
Cuts at: 1275 1525 1555 1920 1950 2394 2424 2859
Size: 250 30 365 30 444 30 435
Fragments arranged by size:
483 466 444 435 365 250 135 71
30 30 30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 157 373 466 490 562 594 679 739
Size: 157 216 93 24 72 32 85 60
Cuts at: 739 859 1048 1551 1564 1567 2608 2617 2859
Size: 120 189 503 13 3 1041 9 242
Fragments arranged by size:
1041 503 242 216 189 157 120 93
85 72 60 32 24 13 9 3
BseYI C'CCAG_C
Cuts at: 0 953 1095 2273 2859
Size: 953 142 1178 586
Fragments arranged by size:
1178 953 586 142
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2287 2859
Size: 2287 572
BsiEI CG_ry'CG
Cuts at: 0 2289 2859
Size: 2289 570
BsiHKAI G_wGCw'C
Cuts at: 0 2048 2206 2404 2859
Size: 2048 158 198 455
Fragments arranged by size:
2048 455 198 158
BsmBI CGTCTCn'nnnn_
Cuts at: 0 262 1082 1480 2517 2859
Size: 262 820 398 1037 342
Fragments arranged by size:
1037 820 398 342 262
Bsp1286I G_dGCh'C
Cuts at: 0 39 560 1465 1645 2048 2206 2219 2296
Size: 39 521 905 180 403 158 13 77
Cuts at: 2296 2404 2410 2583 2630 2859
Size: 108 6 173 47 229
Fragments arranged by size:
905 521 403 229 180 173 158 108
77 47 39 13 6
BspEI T'CCGG_A
Cuts at: 0 114 1049 1835 2859
Size: 114 935 786 1024
Fragments arranged by size:
1024 935 786 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 447 866 1715 2859
Size: 447 419 849 1144
Fragments arranged by size:
1144 849 447 419
BsrBI CCG'CTC
Cuts at: 0 369 388 1437 1700 2238 2859
Size: 369 19 1049 263 538 621
Fragments arranged by size:
1049 621 538 369 263 19
BsrFI r'CCGG_y
Cuts at: 0 1669 2121 2284 2859
Size: 1669 452 163 575
Fragments arranged by size:
1669 575 452 163
BssSI C'ACGA_G
Cuts at: 0 2218 2403 2527 2859
Size: 2218 185 124 332
Fragments arranged by size:
2218 332 185 124
BstEII G'GTnAC_C
Cuts at: 0 1821 1900 2859
Size: 1821 79 959
Fragments arranged by size:
1821 959 79
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2124 2664 2769 2859
Size: 2124 540 105 90
Fragments arranged by size:
2124 540 105 90
BstYI r'GATC_y
Cuts at: 0 1246 2773 2859
Size: 1246 1527 86
Fragments arranged by size:
1527 1246 86
Bsu36I CC'TnA_GG
Cuts at: 0 2455 2672 2859
Size: 2455 217 187
Fragments arranged by size:
2455 217 187
BtgI C'CryG_G
Cuts at: 0 60 1109 2143 2765 2859
Size: 60 1049 1034 622 94
Fragments arranged by size:
1049 1034 622 94 60
BtsI GCAGTG_nn'
Cuts at: 0 911 2859
Size: 911 1948
DrdI GACnn_nn'nnGTC
Cuts at: 0 2505 2509 2859
Size: 2505 4 350
Fragments arranged by size:
2505 350 4
EaeI y'GGCC_r
Cuts at: 0 306 383 396 809 1295 2126 2286 2555
Size: 306 77 13 413 486 831 160 269
Cuts at: 2555 2859
Size: 304
Fragments arranged by size:
831 486 413 306 304 269 160 77
13
EagI C'GGCC_G
Cuts at: 0 2286 2859
Size: 2286 573
EarI CTCTTCn'nnn_
Cuts at: 0 260 580 641 656 1160 2859
Size: 260 320 61 15 504 1699
Fragments arranged by size:
1699 504 320 260 61 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1597 2333 2760 2859
Size: 1597 736 427 99
Fragments arranged by size:
1597 736 427 99
EcoICRI GAG'CTC
Cuts at: 0 2046 2859
Size: 2046 813
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 212 469 508 721 1006 1060 1163
Size: 160 52 257 39 213 285 54 103
Cuts at: 1163 1504 1555 1674 1705 1922 2021 2069 2859
Size: 341 51 119 31 217 99 48 790
Fragments arranged by size:
790 341 285 257 217 213 160 119
103 99 54 52 51 48 39 31
EcoNI CCTnn'n_nnAGG
Cuts at: 0 47 139 661 2859
Size: 47 92 522 2198
Fragments arranged by size:
2198 522 92 47
EcoO109I rG'GnC_Cy
Cuts at: 0 2611 2627 2676 2859
Size: 2611 16 49 183
Fragments arranged by size:
2611 183 49 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1221 2859
Size: 1221 1638
FseI GG_CCGG'CC
Cuts at: 0 2288 2859
Size: 2288 571
FspI TGC'GCA
Cuts at: 0 903 1147 1600 2859
Size: 903 244 453 1259
Fragments arranged by size:
1259 903 453 244
HaeII r_GCGC'y
Cuts at: 0 542 1086 1495 2859
Size: 542 544 409 1364
Fragments arranged by size:
1364 544 542 409
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 466 498 547 579 1159 1191 1245 1277
Size: 466 32 49 32 580 32 54 32
Cuts at: 1277 2570 2602 2603 2635 2778 2810 2859
Size: 1293 32 1 32 143 32 49
Fragments arranged by size:
1293 580 466 143 54 49 49 32
32 32 32 32 32 32 1
HincII GTy'rAC
Cuts at: 0 1851 2859
Size: 1851 1008
HindIII A'AGCT_T
Cuts at: 0 896 2859
Size: 896 1963
KpnI G_GTAC'C
Cuts at: 0 2671 2859
Size: 2671 188
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1142 1307 2540 2859
Size: 1142 165 1233 319
Fragments arranged by size:
1233 1142 319 165
MscI TGG'CCA
Cuts at: 0 308 811 2128 2557 2859
Size: 308 503 1317 429 302
Fragments arranged by size:
1317 503 429 308 302
MslI CAynn'nnrTG
Cuts at: 0 1012 2122 2767 2785 2821 2859
Size: 1012 1110 645 18 36 38
Fragments arranged by size:
1110 1012 645 38 36 18
MspA1I CmG'CkG
Cuts at: 0 150 699 741 957 1319 1604 1737 2859
Size: 150 549 42 216 362 285 133 1122
Fragments arranged by size:
1122 549 362 285 216 150 133 42
NaeI GCC'GGC
Cuts at: 0 2286 2859
Size: 2286 573
NcoI C'CATG_G
Cuts at: 0 1109 2765 2859
Size: 1109 1656 94
Fragments arranged by size:
1656 1109 94
NgoMIV G'CCGG_C
Cuts at: 0 2284 2859
Size: 2284 575
NspI r_CATG'y
Cuts at: 0 1686 1986 2784 2859
Size: 1686 300 798 75
Fragments arranged by size:
1686 798 300 75
PasI CC'CwG_GG
Cuts at: 0 1638 2211 2859
Size: 1638 573 648
Fragments arranged by size:
1638 648 573
PflMI CCAn_nnn'nTGG
Cuts at: 0 933 1095 1482 1578 2092 2859
Size: 933 162 387 96 514 767
Fragments arranged by size:
933 767 514 387 162 96
PfoI T'CCnGG_A
Cuts at: 0 1571 1799 2859
Size: 1571 228 1060
Fragments arranged by size:
1571 1060 228
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1513 1525 1545 1557 2859
Size: 1513 12 20 12 1302
Fragments arranged by size:
1513 1302 20 12 12
PpuMI rG'GwC_Cy
Cuts at: 0 2611 2859
Size: 2611 248
PsiI TTA'TAA
Cuts at: 0 161 2859
Size: 161 2698
PspOMI G'GGCC_C
Cuts at: 0 2626 2859
Size: 2626 233
PstI C_TGCA'G
Cuts at: 0 1160 2726 2859
Size: 1160 1566 133
Fragments arranged by size:
1566 1160 133
PvuII CAG'CTG
Cuts at: 0 150 699 741 1319 2859
Size: 150 549 42 578 1540
Fragments arranged by size:
1540 578 549 150 42
SacI G_AGCT'C
Cuts at: 0 2048 2859
Size: 2048 811
SanDI GG'GwC_CC
Cuts at: 0 2611 2859
Size: 2611 248
SexAI A'CCwGG_T
Cuts at: 0 1229 2859
Size: 1229 1630
SfcI C'TryA_G
Cuts at: 0 1156 2722 2859
Size: 1156 1566 137
Fragments arranged by size:
1566 1156 137
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2634 2859
Size: 2634 225
SgrAI Cr'CCGG_yG
Cuts at: 0 2121 2859
Size: 2121 738
SmaI CCC'GGG
Cuts at: 0 2633 2859
Size: 2633 226
SmlI C'TyrA_G
Cuts at: 0 355 2859
Size: 355 2504
SphI G_CATG'C
Cuts at: 0 2784 2859
Size: 2784 75
StuI AGG'CCT
Cuts at: 0 2663 2859
Size: 2663 196
StyI C'CwwG_G
Cuts at: 0 1109 1118 1788 1826 2765 2859
Size: 1109 9 670 38 939 94
Fragments arranged by size:
1109 939 670 94 38 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1632 1964 2382 2515 2815 2859
Size: 1632 332 418 133 300 44
Fragments arranged by size:
1632 418 332 300 133 44
TatI w'GTAC_w
Cuts at: 0 675 1865 2859
Size: 675 1190 994
Fragments arranged by size:
1190 994 675
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 96 128 2098 2130 2394 2426 2859
Size: 96 32 1970 32 264 32 433
Fragments arranged by size:
1970 433 264 96 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2536 2859
Size: 2536 323
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1116 1786 2859
Size: 1116 670 1073
Fragments arranged by size:
1116 1073 670
XmaI C'CCGG_G
Cuts at: 0 2631 2859
Size: 2631 228
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrFI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI FspI HaeII Hin4I HincII HindIII KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NspI
PasI PflMI PfoI PpiI PpuMI PsiI PspOMI PstI
PvuII SacI SanDI SexAI SfcI SfiI SgrAI SmaI
SmlI SphI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AflII AgeI AhdI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BmrI BmtI BsaAI BsaBI BsiWI BsmI
BspHI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraI DraIII EcoRI EcoRV FspAI HpaI
KasI MfeI MluI NarI NdeI NheI NotI NruI
NsiI PacI PciI PmeI PmlI PshAI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SfoI
SgfI SnaBI SpeI SrfI SspI SwaI XbaI XhoI
XmnI ZraI