(Circular) (Six-base) MAPSORT of: pFN21AA0966.seq Check: 366 from: 1 to: 8101 pFN21AA0966 8101 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5911 278 Size: 53 83 186 936 4375 2468 Fragments arranged by size: 4375 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3138 5444 1937 Size: 1201 2306 4594 Fragments arranged by size: 4594 2306 1201 Acc65I G'GTAC_C Cuts at: 5422 5422 Size: 8101 AclI AA'CG_TT Cuts at: 1563 6228 6601 1563 Size: 4665 373 3063 Fragments arranged by size: 4665 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2417 2525 3200 3590 4157 4170 4545 4743 Size: 785 108 675 390 567 13 375 198 Cuts at: 4743 5166 6164 7548 7816 1632 Size: 423 998 1384 268 1917 Fragments arranged by size: 1917 1384 998 785 675 567 423 390 375 268 198 108 13 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7904 AflIII A'CryG_T Cuts at: 1203 4554 7000 7977 1203 Size: 3351 2446 977 1327 Fragments arranged by size: 3351 2446 1327 977 AgeI A'CCGG_T Cuts at: 6932 6932 Size: 8101 AhdI GACnn_n'nnGTC Cuts at: 6830 6830 Size: 8101 AleI CACnn'nnGTG Cuts at: 1166 3401 4517 1166 Size: 2235 1116 4750 Fragments arranged by size: 4750 2235 1116 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4100 5121 1184 Size: 2916 1021 4164 Fragments arranged by size: 4164 2916 1021 AlwNI CAG_nnn'CTG Cuts at: 3445 4544 5324 5844 7416 3445 Size: 1099 780 520 1572 4130 Fragments arranged by size: 4130 1572 1099 780 520 ApaI G_GGCC'C Cuts at: 3871 3871 Size: 8101 ApaLI G'TGCA_C Cuts at: 6158 7314 6158 Size: 1156 6945 ApoI r'AATT_y Cuts at: 1493 2166 4327 4343 4589 4600 5410 5636 5788 Size: 673 2161 16 246 11 810 226 152 Cuts at: 5788 7897 1493 Size: 2109 1697 Fragments arranged by size: 2161 2109 1697 810 673 246 226 152 16 11 AseI AT'TA_AT Cuts at: 160 1032 5334 6655 160 Size: 872 4302 1321 1606 Fragments arranged by size: 4302 1606 1321 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 AvaI C'yCGr_G Cuts at: 1870 1943 5426 1870 Size: 73 3483 4545 Fragments arranged by size: 4545 3483 73 BamHI G'GATC_C Cuts at: 5431 5798 5431 Size: 367 7734 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5422 6877 618 Size: 325 217 627 3635 1455 1842 Fragments arranged by size: 3635 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 2322 3871 5420 1391 Size: 622 309 1549 1549 4072 Fragments arranged by size: 4072 1549 1549 622 309 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2405 4281 4551 4623 928 Size: 971 506 1876 270 72 4406 Fragments arranged by size: 4406 1876 971 506 270 72 BbvCI CC'TCA_GC Cuts at: 4693 4693 Size: 8101 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6292 6326 1903 Size: 34 4355 34 3678 Fragments arranged by size: 4355 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5993 7203 5993 Size: 1210 6891 BclI T'GATC_A Cuts at: 1548 1548 Size: 8101 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2079 2608 6712 Size: 107 122 71 631 605 407 529 4104 Cuts at: 6712 136 Size: 1525 Fragments arranged by size: 4104 1525 631 605 529 407 122 107 71 BglII A'GATC_T Cuts at: 2182 2656 3721 4937 8096 2182 Size: 474 1065 1216 3159 2187 Fragments arranged by size: 3159 2187 1216 1065 474 BlpI GC'TnA_GC Cuts at: 5518 5518 Size: 8101 Bme1580I G_kGCm'C Cuts at: 1790 3085 3871 6162 7318 1790 Size: 1295 786 2291 1156 2573 Fragments arranged by size: 2573 2291 1295 1156 786 BmgBI CAC'GTC Cuts at: 1318 3916 1318 Size: 2598 5503 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2592 4714 5832 Size: 416 53 83 399 24 1175 2122 1118 Cuts at: 5832 6790 442 Size: 958 1753 Fragments arranged by size: 2122 1753 1175 1118 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4631 1056 Size: 3575 4526 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2072 2661 5301 6761 1239 Size: 833 589 2640 1460 2579 Fragments arranged by size: 2640 2579 1460 833 589 Bpu10I CC'TnA_GC Cuts at: 4693 4693 Size: 8101 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2330 4268 5171 6226 7091 7389 2330 Size: 1938 903 1055 865 298 3042 Fragments arranged by size: 3042 1938 1055 903 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2235 2777 4299 4925 6764 882 Size: 617 736 542 1522 626 1839 2219 Fragments arranged by size: 2219 1839 1522 736 626 617 542 BsaAI yAC'GTr Cuts at: 493 1204 3955 5892 493 Size: 711 2751 1937 2702 Fragments arranged by size: 2751 2702 1937 711 BsaBI GATnn'nnATC Cuts at: 8095 8095 Size: 8101 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5908 6290 6925 275 Size: 53 83 186 936 4375 382 635 1451 Fragments arranged by size: 4375 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6534 6932 7206 7353 7764 6534 Size: 398 274 147 411 6871 Fragments arranged by size: 6871 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 5161 5191 1178 Size: 30 165 30 3758 30 4088 Fragments arranged by size: 4088 3758 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2497 2907 1186 Size: 1311 410 6380 Fragments arranged by size: 6380 1311 410 BseYI C'CCAG_C Cuts at: 3335 3554 3864 7304 7800 3335 Size: 219 310 3440 496 3636 Fragments arranged by size: 3636 3440 496 310 219 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2086 2588 2741 4070 1163 Size: 573 350 502 153 1329 5194 Fragments arranged by size: 5194 1329 573 502 350 153 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2599 5551 6312 7340 1540 Size: 275 186 598 2952 761 1028 2301 Fragments arranged by size: 2952 2301 1028 761 598 275 186 BsiHKAI G_wGCw'C Cuts at: 2013 2280 2322 2582 3243 6162 6247 7318 2013 Size: 267 42 260 661 2919 85 1071 2796 Fragments arranged by size: 2919 2796 1071 661 267 260 85 42 BsmI GAATG_Cn' Cuts at: 2880 3705 5098 5612 5705 2880 Size: 825 1393 514 93 5276 Fragments arranged by size: 5276 1393 825 514 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3906 3906 Size: 8101 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2280 2322 2582 3085 3243 3871 Size: 399 223 267 42 260 503 158 628 Cuts at: 3871 5420 6162 6247 7318 1391 Size: 1549 742 85 1071 2174 Fragments arranged by size: 2174 1549 1071 742 628 503 399 267 260 223 158 85 42 BspEI T'CCGG_A Cuts at: 7764 7764 Size: 8101 BspHI T'CATG_A Cuts at: 5990 7720 5990 Size: 1730 6371 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5456 844 Size: 4612 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5988 1394 Size: 429 4165 3507 Fragments arranged by size: 4165 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4179 6596 6770 65 Size: 1183 2876 55 2417 174 1396 Fragments arranged by size: 2876 2417 1396 1183 174 55 BsrFI r'CCGG_y Cuts at: 1700 1810 6745 6932 1700 Size: 110 4935 187 2869 Fragments arranged by size: 4935 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8101 BssHII G'CGCG_C Cuts at: 1925 2053 2055 1925 Size: 128 2 7971 Fragments arranged by size: 7971 128 2 BssSI C'ACGA_G Cuts at: 2982 6161 7173 2982 Size: 3179 1012 3910 Fragments arranged by size: 3910 3179 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3190 794 Size: 2396 5705 BstBI TT'CG_AA Cuts at: 3090 5227 6905 3090 Size: 2137 1678 4286 Fragments arranged by size: 4286 2137 1678 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8101 BstXI CCAn_nnnn'nTGG Cuts at: 2137 2926 4870 2137 Size: 789 1944 5368 Fragments arranged by size: 5368 1944 789 BstYI r'GATC_y Cuts at: 1972 2182 2305 2656 3721 4937 5431 5798 6183 Size: 210 123 351 1065 1216 494 367 385 Cuts at: 6183 6200 7652 7738 7750 8096 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1216 1065 494 385 367 351 346 210 123 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2301 2793 2301 Size: 492 7609 BtgI C'CryG_G Cuts at: 513 1065 2004 2189 3038 3856 4212 5235 5275 Size: 552 939 185 849 818 356 1023 40 Cuts at: 5275 513 Size: 3339 Fragments arranged by size: 3339 1023 939 849 818 552 356 185 40 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3183 3403 5022 487 Size: 683 56 1957 220 1619 3566 Fragments arranged by size: 3566 1957 1619 683 220 56 BtsI GCAGTG_nn' Cuts at: 3241 5298 5617 6411 6431 3241 Size: 2057 319 794 20 4911 Fragments arranged by size: 4911 2057 794 319 20 ClaI AT'CG_AT Cuts at: 1554 3022 1554 Size: 1468 6633 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4036 4071 5061 5096 529 Size: 35 3472 35 990 35 3534 Fragments arranged by size: 3534 3472 990 35 35 35 DraI TTT'AAA Cuts at: 5405 5405 Size: 8101 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2249 7108 809 Size: 507 933 4859 1802 Fragments arranged by size: 4859 1802 933 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5033 5548 6437 Size: 54 1425 50 275 18 3203 515 889 Cuts at: 6437 8066 8 Size: 1629 43 Fragments arranged by size: 3203 1629 1425 889 515 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5548 1537 Size: 275 3736 4090 Fragments arranged by size: 4090 3736 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2018 2490 2884 3145 4146 4250 5303 6031 Size: 665 472 394 261 1001 104 1053 728 Cuts at: 6031 1353 Size: 3423 Fragments arranged by size: 3423 1053 1001 728 665 472 394 261 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6688 7062 7208 387 Size: 1064 138 5099 374 146 1280 Fragments arranged by size: 5099 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2011 2320 2011 Size: 309 7792 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2072 2417 2525 2661 3200 3590 4157 Size: 393 440 345 108 136 539 390 567 Cuts at: 4157 4170 4545 4743 5166 5301 6164 6761 7548 Size: 13 375 198 423 135 863 597 787 Cuts at: 7548 7816 1239 Size: 268 1524 Fragments arranged by size: 1524 863 787 597 567 539 440 423 393 390 375 345 268 198 136 135 108 13 EcoNI CCTnn'n_nnAGG Cuts at: 2042 2042 Size: 8101 EcoO109I rG'GnC_Cy Cuts at: 3868 4037 3868 Size: 169 7932 EcoRI G'AATT_C Cuts at: 4589 5410 5788 4589 Size: 821 378 6902 Fragments arranged by size: 6902 821 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3209 3595 4187 4490 3209 Size: 386 592 303 6820 Fragments arranged by size: 6820 592 386 303 FspI TGC'GCA Cuts at: 6607 6607 Size: 8101 HaeII r_GCGC'y Cuts at: 1678 4700 7248 1678 Size: 3022 2548 2531 Fragments arranged by size: 3022 2548 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2510 2542 2581 2613 2641 Size: 32 135 32 938 32 39 32 28 Cuts at: 2641 2659 2673 2691 2776 2808 3550 3582 3636 Size: 18 14 18 85 32 742 32 54 Cuts at: 3636 3668 3706 3738 4556 4588 4922 4954 5014 Size: 32 38 32 818 32 334 32 60 Cuts at: 5014 5046 6742 6774 6816 6848 1373 Size: 32 1696 32 42 32 2626 Fragments arranged by size: 2626 1696 938 818 742 334 135 85 60 54 42 39 38 32 32 32 32 32 32 32 32 32 32 32 32 32 28 18 18 14 HincII GTy'rAC Cuts at: 669 1657 1938 4444 5445 5691 669 Size: 988 281 2506 1001 246 3079 Fragments arranged by size: 3079 2506 1001 988 281 246 HindIII A'AGCT_T Cuts at: 748 3759 748 Size: 3011 5090 HpaI GTT'AAC Cuts at: 4444 5691 4444 Size: 1247 6854 KpnI G_GTAC'C Cuts at: 5426 5426 Size: 8101 MfeI C'AATT_G Cuts at: 5700 5700 Size: 8101 MluI A'CGCG_T Cuts at: 7977 7977 Size: 8101 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2119 2457 7215 7399 1305 Size: 814 338 4758 184 2007 Fragments arranged by size: 4758 2007 814 338 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 6279 Fragments arranged by size: 6279 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3401 3710 4517 4584 4782 6059 6418 Size: 648 2235 309 807 67 198 1277 359 Cuts at: 6418 6577 518 Size: 159 2042 Fragments arranged by size: 2235 2042 1277 807 648 359 309 198 159 67 MspA1I CmG'CkG Cuts at: 1606 3040 3445 3563 5517 6194 7342 7587 1606 Size: 1434 405 118 1954 677 1148 245 2120 Fragments arranged by size: 2120 1954 1434 1148 677 405 245 118 NcoI C'CATG_G Cuts at: 513 1065 2004 2189 3856 4212 5235 5275 513 Size: 552 939 185 1667 356 1023 40 3339 Fragments arranged by size: 3339 1667 1023 939 552 356 185 40 NdeI CA'TA_TG Cuts at: 387 387 Size: 8101 NheI G'CTAG_C Cuts at: 1052 4627 1052 Size: 3575 4526 NotI GC'GGCC_GC Cuts at: 5548 5548 Size: 8101 NruI TCG'CGA Cuts at: 3422 3422 Size: 8101 NspI r_CATG'y Cuts at: 1132 1229 4081 4558 5143 5459 7004 1132 Size: 97 2852 477 585 316 1545 2229 Fragments arranged by size: 2852 2229 1545 585 477 316 97 PasI CC'CwG_GG Cuts at: 1348 4318 1348 Size: 2970 5131 PciI A'CATG_T Cuts at: 4554 7000 4554 Size: 2446 5655 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 8101 PmeI GTTT'AAAC Cuts at: 5405 5405 Size: 8101 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6170 6202 7683 7715 6170 Size: 32 1481 32 6556 Fragments arranged by size: 6556 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8101 PsiI TTA'TAA Cuts at: 5671 5671 Size: 8101 PspOMI G'GGCC_C Cuts at: 3867 3867 Size: 8101 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3465 3497 3465 Size: 32 8069 PstI C_TGCA'G Cuts at: 830 2043 4197 5453 830 Size: 1213 2154 1256 3478 Fragments arranged by size: 3478 2154 1256 1213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8101 PvuII CAG'CTG Cuts at: 3445 3563 3445 Size: 118 7983 SacI G_AGCT'C Cuts at: 2013 2322 2013 Size: 309 7792 SacII CC_GC'GG Cuts at: 3041 3041 Size: 8101 SalI G'TCGA_C Cuts at: 1936 5443 1936 Size: 3507 4594 SapI GCTCTTCn'nnn_ Cuts at: 2018 4250 2018 Size: 2232 5869 SbfI CC_TGCA'GG Cuts at: 5453 5453 Size: 8101 ScaI AGT'ACT Cuts at: 6349 8061 6349 Size: 1712 6389 SfcI C'TryA_G Cuts at: 826 1045 2039 4090 4193 5449 6584 7265 7456 Size: 219 994 2051 103 1256 1135 681 191 Cuts at: 7456 826 Size: 1471 Fragments arranged by size: 2051 1471 1256 1135 994 681 219 191 103 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8101 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8101 SmaI CCC'GGG Cuts at: 1872 5428 1872 Size: 3556 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2309 4247 5150 6205 7106 7368 Size: 197 926 366 1938 903 1055 901 262 Cuts at: 7368 820 Size: 1553 Fragments arranged by size: 1938 1553 1055 926 903 901 366 262 197 SnaBI TAC'GTA Cuts at: 493 5892 493 Size: 5399 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8101 SphI G_CATG'C Cuts at: 1132 4081 5143 5459 1132 Size: 2949 1062 316 3774 Fragments arranged by size: 3774 2949 1062 316 SspI AAT'ATT Cuts at: 5 52 4393 6025 5 Size: 47 4341 1632 2081 Fragments arranged by size: 4341 2081 1632 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8101 StyI C'CwwG_G Cuts at: 513 1065 2004 2022 2189 2710 3741 3856 4212 Size: 552 939 18 167 521 1031 115 356 Cuts at: 4212 4303 5235 5275 5540 513 Size: 91 932 40 265 3074 Fragments arranged by size: 3074 1031 939 932 552 521 356 265 167 115 91 40 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2640 2838 4704 6128 6145 6298 6483 Size: 1255 1060 198 1866 1424 17 153 185 Cuts at: 6483 6928 325 Size: 445 1498 Fragments arranged by size: 1866 1498 1424 1255 1060 445 198 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6347 8059 96 Size: 274 80 33 51 1443 4370 1712 138 Fragments arranged by size: 4370 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3352 3384 8061 8093 3352 Size: 32 4677 32 3360 Fragments arranged by size: 4677 3360 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4005 1316 Size: 2689 5412 XbaI T'CTAG_A Cuts at: 4009 5437 4009 Size: 1428 6673 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3424 4210 3424 Size: 786 7315 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8101 XmaI C'CCGG_G Cuts at: 1870 5426 1870 Size: 3556 4545 XmnI GAAnn'nnTTC Cuts at: 2510 6230 2510 Size: 3720 4381 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5909 276 Size: 53 83 186 936 4375 2468 Fragments arranged by size: 4375 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI RsrII SanDI SexAI SfiI SfoI SrfI SwaI