(Circular) (Six-base) MAPSORT of: pFN21AA0954.seq Check: 8658 from: 1 to: 8158 pFN21AA0954 8158 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2446 2446 Size: 8158 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5968 278 Size: 53 83 186 936 4432 2468 Fragments arranged by size: 4432 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4398 5501 1937 Size: 2461 1103 4594 Fragments arranged by size: 4594 2461 1103 Acc65I G'GTAC_C Cuts at: 5479 5479 Size: 8158 AclI AA'CG_TT Cuts at: 1563 6285 6658 1563 Size: 4722 373 3063 Fragments arranged by size: 4722 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3317 3430 4159 4321 6221 7605 7873 1632 Size: 1685 113 729 162 1900 1384 268 1917 Fragments arranged by size: 1917 1900 1685 1384 729 268 162 113 AfeI AGC'GCT Cuts at: 2039 4106 4292 2039 Size: 2067 186 5905 Fragments arranged by size: 5905 2067 186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7961 AflIII A'CryG_T Cuts at: 1203 3784 4629 7057 8034 1203 Size: 2581 845 2428 977 1327 Fragments arranged by size: 2581 2428 1327 977 845 AgeI A'CCGG_T Cuts at: 5316 6989 5316 Size: 1673 6485 AhdI GACnn_n'nnGTC Cuts at: 6887 6887 Size: 8158 AleI CACnn'nnGTG Cuts at: 1166 3422 1166 Size: 2256 5902 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3605 5186 1184 Size: 2421 1581 4156 Fragments arranged by size: 4156 2421 1581 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5189 5221 5189 Size: 32 8126 AlwNI CAG_nnn'CTG Cuts at: 2671 2713 2725 2815 4610 5068 5901 7473 2671 Size: 42 12 90 1795 458 833 1572 3356 Fragments arranged by size: 3356 1795 1572 833 458 90 42 12 ApaI G_GGCC'C Cuts at: 2364 2924 4957 4969 4988 5008 2364 Size: 560 2033 12 19 20 5514 Fragments arranged by size: 5514 2033 560 20 19 12 ApaLI G'TGCA_C Cuts at: 3722 3842 6215 7371 3722 Size: 120 2373 1156 4509 Fragments arranged by size: 4509 2373 1156 120 ApoI r'AATT_y Cuts at: 1493 5074 5348 5467 5693 5845 7954 1493 Size: 3581 274 119 226 152 2109 1697 Fragments arranged by size: 3581 2109 1697 274 226 152 119 AseI AT'TA_AT Cuts at: 160 1032 6712 160 Size: 872 5680 1606 Fragments arranged by size: 5680 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8158 AvaI C'yCGr_G Cuts at: 1870 1943 2254 2281 2347 2428 2874 2917 3263 Size: 73 311 27 66 81 446 43 346 Cuts at: 3263 3860 4165 5483 1870 Size: 597 305 1318 4545 Fragments arranged by size: 4545 1318 597 446 346 311 305 81 73 66 43 27 BamHI G'GATC_C Cuts at: 5488 5855 5488 Size: 367 7791 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2066 2259 2291 2354 2674 Size: 325 217 627 279 193 32 63 320 Cuts at: 2674 2970 2981 3652 4169 5162 5306 5479 6934 Size: 296 11 671 517 993 144 173 1455 Cuts at: 6934 618 Size: 1842 Fragments arranged by size: 1842 1455 993 671 627 517 325 320 296 279 217 193 173 144 63 32 11 BanII G_rGCy'C Cuts at: 1391 2079 2234 2364 2382 2435 2469 2637 2748 Size: 688 155 130 18 53 34 168 111 Cuts at: 2748 2881 2924 3246 3749 4957 4969 4988 4998 Size: 133 43 322 503 1208 12 19 10 Cuts at: 4998 5008 5477 1391 Size: 10 469 4072 Fragments arranged by size: 4072 1208 688 503 469 322 168 155 133 130 111 53 43 34 19 18 12 10 10 BbeI G_GCGC'C Cuts at: 2263 4173 2263 Size: 1910 6248 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3658 928 Size: 971 1759 5428 Fragments arranged by size: 5428 1759 971 BbvCI CC'TCA_GC Cuts at: 5177 5177 Size: 8158 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2855 2889 6349 6383 1903 Size: 34 918 34 3460 34 3678 Fragments arranged by size: 3678 3460 918 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6050 7260 6050 Size: 1210 6948 BclI T'GATC_A Cuts at: 1548 2826 1548 Size: 1278 6880 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2171 2893 3024 Size: 107 122 71 631 605 499 722 131 Cuts at: 3024 3530 3684 6769 136 Size: 506 154 3085 1525 Fragments arranged by size: 3085 1525 722 631 605 506 499 154 131 122 107 71 BglII A'GATC_T Cuts at: 3570 3933 8153 3570 Size: 363 4220 3575 Fragments arranged by size: 4220 3575 363 BlpI GC'TnA_GC Cuts at: 2376 2742 4370 4992 5575 2376 Size: 366 1628 622 583 4959 Fragments arranged by size: 4959 1628 622 583 366 Bme1580I G_kGCm'C Cuts at: 1790 2071 2357 2364 2520 2679 2924 2986 3649 Size: 281 286 7 156 159 245 62 663 Cuts at: 3649 3657 3726 3846 4487 4957 4969 4988 5008 Size: 8 69 120 641 470 12 19 20 Cuts at: 5008 5046 5309 6219 7375 1790 Size: 38 263 910 1156 2573 Fragments arranged by size: 2573 1156 910 663 641 470 286 281 263 245 159 156 120 69 62 38 20 19 12 8 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8158 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3349 5089 5889 Size: 416 53 83 399 24 1932 1740 800 Cuts at: 5889 6847 442 Size: 958 1753 Fragments arranged by size: 1932 1753 1740 958 800 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8158 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2146 2461 2762 4985 2146 Size: 315 301 2223 5319 Fragments arranged by size: 5319 2223 315 301 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2123 2145 2194 2532 2549 3338 3512 Size: 854 30 22 49 338 17 789 174 Cuts at: 3512 4100 4121 4129 4889 4955 5090 5091 6818 Size: 588 21 8 760 66 135 1 1727 Cuts at: 6818 1239 Size: 2579 Fragments arranged by size: 2579 1727 854 789 760 588 338 174 135 66 49 30 22 21 17 8 1 Bpu10I CC'TnA_GC Cuts at: 2228 3408 3918 4765 5177 2228 Size: 1180 510 847 412 5209 Fragments arranged by size: 5209 1180 847 510 412 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3257 4133 6283 7148 7446 3257 Size: 876 2150 865 298 3969 Fragments arranged by size: 3969 2150 876 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3446 6821 882 Size: 617 1947 3375 2219 Fragments arranged by size: 3375 2219 1947 617 BsaAI yAC'GTr Cuts at: 493 1204 2509 3787 4366 5949 493 Size: 711 1305 1278 579 1583 2702 Fragments arranged by size: 2702 1583 1305 1278 711 579 BsaBI GATnn'nnATC Cuts at: 5084 8152 5084 Size: 3068 5090 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2260 3180 3260 4170 Size: 53 83 186 936 727 920 80 910 Cuts at: 4170 5965 6347 6982 275 Size: 1795 382 635 1451 Fragments arranged by size: 1795 1451 936 920 910 727 635 382 186 83 80 53 BsaWI w'CCGG_w Cuts at: 3363 3959 4267 5316 6591 6989 7263 7410 7821 Size: 596 308 1049 1275 398 274 147 411 Cuts at: 7821 3363 Size: 3700 Fragments arranged by size: 3700 1275 1049 596 411 398 308 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2417 2447 3954 3984 5189 Size: 30 165 30 1014 30 1507 30 1205 Cuts at: 5189 5219 5265 5295 1178 Size: 30 46 30 4041 Fragments arranged by size: 4041 1507 1205 1014 165 46 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2120 4400 5191 5194 5273 1186 Size: 934 2280 791 3 79 4071 Fragments arranged by size: 4071 2280 934 791 79 3 BseYI C'CCAG_C Cuts at: 2451 2697 2792 3235 4455 4709 4745 4751 4772 Size: 246 95 443 1220 254 36 6 21 Cuts at: 4772 4931 5377 5412 7361 7857 2451 Size: 159 446 35 1949 496 2752 Fragments arranged by size: 2752 1949 1220 496 446 443 254 246 159 95 36 35 21 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2699 2849 3287 3425 3809 5156 5248 Size: 573 963 150 438 138 384 1347 92 Cuts at: 5248 1163 Size: 4073 Fragments arranged by size: 4073 1347 963 573 438 384 150 138 92 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3679 3704 5608 6369 7397 1540 Size: 275 186 1678 25 1904 761 1028 2301 Fragments arranged by size: 2301 1904 1678 1028 761 275 186 25 BsiHKAI G_wGCw'C Cuts at: 2748 3726 3846 5107 6219 6304 7375 2748 Size: 978 120 1261 1112 85 1071 3531 Fragments arranged by size: 3531 1261 1112 1071 978 120 85 BsmI GAATG_Cn' Cuts at: 5669 5762 5669 Size: 93 8065 BsmBI CGTCTCn'nnnn_ Cuts at: 2015 4076 2015 Size: 2061 6097 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2079 2234 2357 2364 2382 2435 Size: 399 281 8 155 123 7 18 53 Cuts at: 2435 2469 2520 2637 2679 2748 2881 2924 2986 Size: 34 51 117 42 69 133 43 62 Cuts at: 2986 3246 3649 3657 3726 3749 3846 4487 4957 Size: 260 403 8 69 23 97 641 470 Cuts at: 4957 4969 4988 4998 5008 5046 5107 5309 5477 Size: 12 19 10 10 38 61 202 168 Cuts at: 5477 6219 6304 7375 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 641 470 403 399 281 260 202 168 155 133 123 117 97 85 69 69 62 61 53 51 43 42 38 34 23 19 18 12 10 10 8 8 7 BspEI T'CCGG_A Cuts at: 4267 7821 4267 Size: 3554 4604 BspHI T'CATG_A Cuts at: 6047 7777 6047 Size: 1730 6428 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2446 3403 3777 5513 844 Size: 1602 957 374 1736 3489 Fragments arranged by size: 3489 1736 1602 957 374 BsrBI CCG'CTC Cuts at: 1394 1823 2911 4764 6045 1394 Size: 429 1088 1853 1281 3507 Fragments arranged by size: 3507 1853 1281 1088 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2943 4311 6653 6827 65 Size: 1183 1695 1368 2342 174 1396 Fragments arranged by size: 2342 1695 1396 1368 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3699 4157 5316 6802 6989 1700 Size: 110 1889 458 1159 1486 187 2869 Fragments arranged by size: 2869 1889 1486 1159 458 187 110 BsrGI T'GTAC_A Cuts at: 96 2619 3222 96 Size: 2523 603 5032 Fragments arranged by size: 5032 2523 603 BssHII G'CGCG_C Cuts at: 1925 3772 1925 Size: 1847 6311 BssSI C'ACGA_G Cuts at: 6218 7230 6218 Size: 1012 7146 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8158 BstBI TT'CG_AA Cuts at: 2046 6962 2046 Size: 4916 3242 BstEII G'GTnAC_C Cuts at: 1184 4576 1184 Size: 3392 4766 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2101 4463 4505 4780 2011 Size: 90 2362 42 275 5389 Fragments arranged by size: 5389 2362 275 90 42 BstYI r'GATC_y Cuts at: 1972 2614 3570 3822 3933 5488 5855 6240 6257 Size: 642 956 252 111 1555 367 385 17 Cuts at: 6257 7709 7795 7807 8153 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1555 1452 956 642 385 367 346 252 111 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3942 5234 3942 Size: 1292 6866 BtgI C'CryG_G Cuts at: 513 1065 2004 2185 2263 2658 3673 4260 4487 Size: 552 939 181 78 395 1015 587 227 Cuts at: 4487 4539 4945 513 Size: 52 406 3726 Fragments arranged by size: 3726 1015 939 587 552 406 395 227 181 78 52 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2213 2868 3642 3898 487 Size: 683 56 987 655 774 256 4747 Fragments arranged by size: 4747 987 774 683 655 256 56 BtsI GCAGTG_nn' Cuts at: 2405 5168 5372 5674 6468 6488 2405 Size: 2763 204 302 794 20 4075 Fragments arranged by size: 4075 2763 794 302 204 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8158 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8123 DraI TTT'AAA Cuts at: 5462 5462 Size: 8158 DraIII CAC_nnn'GTG Cuts at: 3310 3310 Size: 8158 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7165 809 Size: 507 5849 1802 Fragments arranged by size: 5849 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3561 3676 3701 Size: 54 1425 50 275 18 1731 115 25 Cuts at: 3701 4122 4242 4723 4843 4878 4948 5321 5605 Size: 421 120 481 120 35 70 373 284 Cuts at: 5605 6494 8123 8 Size: 889 1629 43 Fragments arranged by size: 1731 1629 1425 889 481 421 373 284 275 120 120 115 70 54 50 43 35 25 18 EagI C'GGCC_G Cuts at: 1537 1812 3676 3701 5605 1537 Size: 275 1864 25 1904 4090 Fragments arranged by size: 4090 1904 1864 275 25 EarI CTCTTCn'nnn_ Cuts at: 1353 2762 3812 4220 6088 1353 Size: 1409 1050 408 1868 3423 Fragments arranged by size: 3423 1868 1409 1050 408 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2526 6745 7119 7265 387 Size: 1064 138 937 4219 374 146 1280 Fragments arranged by size: 4219 1280 1064 937 374 146 138 EcoICRI GAG'CTC Cuts at: 2746 2746 Size: 8158 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2123 2145 2194 2532 2549 3317 Size: 393 461 30 22 49 338 17 768 Cuts at: 3317 3338 3430 3512 4100 4121 4129 4159 4321 Size: 21 92 82 588 21 8 30 162 Cuts at: 4321 4889 4955 5090 5091 6221 6818 7605 7873 Size: 568 66 135 1 1130 597 787 268 Cuts at: 7873 1239 Size: 1524 Fragments arranged by size: 1524 1130 787 768 597 588 568 461 393 338 268 162 135 92 82 66 49 30 30 22 21 21 17 8 1 EcoNI CCTnn'n_nnAGG Cuts at: 3002 3002 Size: 8158 EcoO109I rG'GnC_Cy Cuts at: 2361 2564 2921 3268 3322 4230 4390 4769 4965 Size: 203 357 347 54 908 160 379 196 Cuts at: 4965 4966 4985 2361 Size: 1 19 5534 Fragments arranged by size: 5534 908 379 357 347 203 196 160 54 19 1 EcoRI G'AATT_C Cuts at: 5074 5348 5467 5845 5074 Size: 274 119 378 7387 Fragments arranged by size: 7387 378 274 119 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3223 3394 3952 3223 Size: 171 558 7429 Fragments arranged by size: 7429 558 171 FspI TGC'GCA Cuts at: 2480 4379 6664 2480 Size: 1899 2285 3974 Fragments arranged by size: 3974 2285 1899 HaeII r_GCGC'y Cuts at: 1678 2033 2041 2263 2331 3716 4108 4173 4294 Size: 355 8 222 68 1385 392 65 121 Cuts at: 4294 7305 1678 Size: 3011 2531 Fragments arranged by size: 3011 2531 1385 392 355 222 121 68 65 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2417 2449 3954 3986 6799 Size: 32 135 32 845 32 1505 32 2813 Cuts at: 6799 6831 6873 6905 1373 Size: 32 42 32 2626 Fragments arranged by size: 2813 2626 1505 845 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5502 5748 669 Size: 988 281 3564 246 3079 Fragments arranged by size: 3564 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8158 HpaI GTT'AAC Cuts at: 5748 5748 Size: 8158 KasI G'GCGC_C Cuts at: 2259 4169 2259 Size: 1910 6248 KpnI G_GTAC'C Cuts at: 5483 5483 Size: 8158 MfeI C'AATT_G Cuts at: 3812 5757 3812 Size: 1945 6213 MluI A'CGCG_T Cuts at: 8034 8034 Size: 8158 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2106 2157 3624 3789 4546 7272 7456 1305 Size: 801 51 1467 165 757 2726 184 2007 Fragments arranged by size: 2726 2007 1467 801 757 184 165 51 MscI TGG'CCA Cuts at: 10 64 1489 1832 4124 4244 4725 4845 4880 Size: 54 1425 343 2292 120 481 120 35 Cuts at: 4880 5323 10 Size: 443 2845 Fragments arranged by size: 2845 2292 1425 481 443 343 120 120 54 35 MslI CAynn'nnrTG Cuts at: 518 1166 2009 3230 3422 3515 3587 3729 4028 Size: 648 843 1221 192 93 72 142 299 Cuts at: 4028 5063 6116 6475 6634 518 Size: 1035 1053 359 159 2042 Fragments arranged by size: 2042 1221 1053 1035 843 648 359 299 192 159 142 93 72 MspA1I CmG'CkG Cuts at: 1606 2214 2299 2611 3643 5574 6251 7399 7644 Size: 608 85 312 1032 1931 677 1148 245 Cuts at: 7644 1606 Size: 2120 Fragments arranged by size: 2120 1931 1148 1032 677 608 312 245 85 NarI GG'CG_CC Cuts at: 2260 4170 2260 Size: 1910 6248 NcoI C'CATG_G Cuts at: 513 1065 2004 2263 2658 4487 4945 513 Size: 552 939 259 395 1829 458 3726 Fragments arranged by size: 3726 1829 939 552 458 395 259 NdeI CA'TA_TG Cuts at: 387 387 Size: 8158 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8158 NotI GC'GGCC_GC Cuts at: 5605 5605 Size: 8158 NspI r_CATG'y Cuts at: 1132 1229 3235 3472 3835 4633 5516 7061 1132 Size: 97 2006 237 363 798 883 1545 2229 Fragments arranged by size: 2229 2006 1545 883 798 363 237 97 PasI CC'CwG_GG Cuts at: 1348 2237 2902 4463 4901 5008 5302 1348 Size: 889 665 1561 438 107 294 4204 Fragments arranged by size: 4204 1561 889 665 438 294 107 PciI A'CATG_T Cuts at: 4629 7057 4629 Size: 2428 5730 PflMI CCAn_nnn'nTGG Cuts at: 1108 3060 4120 4818 5068 1108 Size: 1952 1060 698 250 4198 Fragments arranged by size: 4198 1952 1060 698 250 PfoI T'CCnGG_A Cuts at: 2100 2100 Size: 8158 PmeI GTTT'AAAC Cuts at: 5462 5462 Size: 8158 PmlI CAC'GTG Cuts at: 2509 3787 2509 Size: 1278 6880 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5189 5221 6227 6259 7740 7772 5189 Size: 32 1006 32 1481 32 5575 Fragments arranged by size: 5575 1481 1006 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4390 4390 Size: 8158 PshAI GACnn'nnGTC Cuts at: 1613 3908 1613 Size: 2295 5863 PsiI TTA'TAA Cuts at: 5728 5728 Size: 8158 PspOMI G'GGCC_C Cuts at: 2360 2920 4953 4965 4984 5004 2360 Size: 560 2033 12 19 20 5514 Fragments arranged by size: 5514 2033 560 20 19 12 PspXI vC'TCGA_Gb Cuts at: 2874 2874 Size: 8158 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3536 3568 5123 5155 3536 Size: 32 1555 32 6539 Fragments arranged by size: 6539 1555 32 32 PstI C_TGCA'G Cuts at: 830 2037 2610 2730 3006 4302 4891 5123 5193 Size: 1207 573 120 276 1296 589 232 70 Cuts at: 5193 5396 5510 830 Size: 203 114 3478 Fragments arranged by size: 3478 1296 1207 589 573 276 232 203 120 114 70 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8158 SacI G_AGCT'C Cuts at: 2748 2748 Size: 8158 SalI G'TCGA_C Cuts at: 1936 5500 1936 Size: 3564 4594 SbfI CC_TGCA'GG Cuts at: 3006 4302 5123 5510 3006 Size: 1296 821 387 5654 Fragments arranged by size: 5654 1296 821 387 ScaI AGT'ACT Cuts at: 5099 6406 8118 5099 Size: 1307 1712 5139 Fragments arranged by size: 5139 1712 1307 SexAI A'CCwGG_T Cuts at: 4392 4392 Size: 8158 SfcI C'TryA_G Cuts at: 826 1045 2033 2606 2726 3002 3847 4298 4887 Size: 219 988 573 120 276 845 451 589 Cuts at: 4887 5119 5189 5392 5506 6641 7322 7513 826 Size: 232 70 203 114 1135 681 191 1471 Fragments arranged by size: 1471 1135 988 845 681 589 573 451 276 232 219 203 191 120 114 70 SfoI GGC'GCC Cuts at: 2261 4171 2261 Size: 1910 6248 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8158 SgrAI Cr'CCGG_yG Cuts at: 1700 5316 1700 Size: 3616 4542 SmaI CCC'GGG Cuts at: 1872 2256 2283 2349 2919 5485 1872 Size: 384 27 66 570 2566 4545 Fragments arranged by size: 4545 2566 570 384 66 27 SmlI C'TyrA_G Cuts at: 820 1017 1943 2874 3272 4148 6262 7163 7425 Size: 197 926 931 398 876 2114 901 262 Cuts at: 7425 820 Size: 1553 Fragments arranged by size: 2114 1553 931 926 901 876 398 262 197 SnaBI TAC'GTA Cuts at: 493 5949 493 Size: 5456 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8158 SphI G_CATG'C Cuts at: 1132 3235 3835 5516 1132 Size: 2103 600 1681 3774 Fragments arranged by size: 3774 2103 1681 600 SspI AAT'ATT Cuts at: 5 52 6082 5 Size: 47 6030 2081 Fragments arranged by size: 6030 2081 47 StuI AGG'CCT Cuts at: 1517 2441 2997 4277 4478 5125 1517 Size: 924 556 1280 201 647 4550 Fragments arranged by size: 4550 1280 924 647 556 201 StyI C'CwwG_G Cuts at: 513 1065 2004 2263 2382 2658 2966 3777 4236 Size: 552 939 259 119 276 308 811 459 Cuts at: 4236 4487 4623 4936 4945 5597 513 Size: 251 136 313 9 652 3074 Fragments arranged by size: 3074 939 811 652 552 459 313 308 276 259 251 136 119 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2711 4687 5303 5315 6185 6202 6355 Size: 1255 1131 1976 616 12 870 17 153 Cuts at: 6355 6540 6985 325 Size: 185 445 1498 Fragments arranged by size: 1976 1498 1255 1131 870 616 445 185 153 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2109 2619 3222 Size: 274 80 33 51 1443 132 510 603 Cuts at: 3222 5097 6404 8116 96 Size: 1875 1307 1712 138 Fragments arranged by size: 1875 1712 1443 1307 603 510 274 138 132 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2768 2800 5265 5297 8118 8150 2768 Size: 32 2465 32 2821 32 2776 Fragments arranged by size: 2821 2776 2465 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3662 3968 1316 Size: 2346 306 5506 Fragments arranged by size: 5506 2346 306 XbaI T'CTAG_A Cuts at: 5494 5494 Size: 8158 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2816 2889 4315 5065 2011 Size: 805 73 1426 750 5104 Fragments arranged by size: 5104 1426 805 750 73 XhoI C'TCGA_G Cuts at: 1943 2874 1943 Size: 931 7227 XmaI C'CCGG_G Cuts at: 1870 2254 2281 2347 2917 5483 1870 Size: 384 27 66 570 2566 4545 Fragments arranged by size: 4545 2566 570 384 66 27 XmnI GAAnn'nnTTC Cuts at: 3206 6287 3206 Size: 3081 5077 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5966 276 Size: 53 83 186 936 4432 2468 Fragments arranged by size: 4432 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PvuII RsrII SacII SanDI SapI SfiI SrfI SwaI