Restriction map for
pFN21AA0910
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# NO CUT ENZYMES ( 79 enzymes) #
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AatII Acc65I AccI AclI AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AvrII BalI
BanI BbeI BclI BglI BlnI
BsiWI BsmBI BspHI BspLU11I BsrBI
BsrFI BssHII Bst1107I BstZ17I Cfr10I
CpoI DraIII DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I FbaI
FseI HaeII HincII HindII HpaI
KasI KpnI KspI MluI MluNI
MscI NaeI NarI NcoI NgoMIV
NotI NruI PacI PinAI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SfiI
SfoI SgrAI SmaI SnaBI Sse8387I
SwaI XcmI XmaCI XmaI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AccIII 20 TCCGGA T^CCGGA
AcyI 1903 GGCGTC G[GA]^CG[CT]C
AflII 865 CTTAAG C^TTAAG
AflIII 2516 ACACGT A^C[GA][CT]GT
Alw44I 3239 GTGCAC G^TGCAC
AocI 3631 CCTCAGG CC^T[ACGT]AGG
ApaLI 3239 GTGCAC G^TGCAC
AvaI 1478 CTCGAG C^[CT]CG[GA]G
AviII 432 TGCGCA TGC^GCA
BamHI 39 GGATCC G^GATCC
BbrPI 2517 CACGTG CAC^GTG
BbvCI 3079 CCTCAGC CCTCAGC(-5/-2)
BcgI 1138 CGAACAAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3472 GGATAC GTATCC(6/5)
BfrI 865 CTTAAG C^TTAAG
BlpI 2554 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2554 GCTGAGC GC^T[ACGT]AGC
BsaAI 2517 CACGTG [CT]AC^GT[GA]
BsaHI 1903 GGCGTC G[GA]^CG[CT]C
BsaWI 20 TCCGGA [AT]^CCGG[AT]
BseAI 20 TCCGGA T^CCGGA
BsiEI 3290 CGGTCG CG[GA][CT]^CG
BsoBI 1478 CTCGAG C^[CT]CG[GA]G
Bsp1407I 2513 TGTACA T^GTACA
BspEI 20 TCCGGA T^CCGGA
BsrGI 2513 TGTACA T^GTACA
BssSI 3503 CTCGTG CACGAG(-5/-1)
BstBI 1136 TTCGAA TT^CGAA
BstEII 2331 GGTAACC G^GT[ACGT]ACC
BstPI 2331 GGTAACC G^GT[ACGT]ACC
Bsu36I 3631 CCTCAGG CC^T[ACGT]AGG
CelII 2554 GCTGAGC GC^T[ACGT]AGC
DraI 2901 TTTAAA TTT^AAA
DrdI 1521 GACATTACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3631 CCTCAGG CC^T[ACGT]AGG
EcoNI 3439 CCTCAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2331 GGTAACC G^GT[ACGT]ACC
EcoRI 369 GAATTC G^AATTC
EcoRV 979 GATATC GAT^ATC
EcoT14I 3111 CCTTGG C^C[AT][AT]GG
EcoT22I 173 ATGCAT ATGCA^T
FspI 432 TGCGCA TGC^GCA
Hin1I 1903 GGCGTC G[GA]^CG[CT]C
MfeI 1757 CAATTG C^AATTG
MroI 20 TCCGGA T^CCGGA
MslI 2164 CATGACTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 2459 CAGCTG C[AC]G^C[GT]G
MunI 1757 CAATTG C^AATTG
NheI 1203 GCTAGC G^CTAGC
NsiI 173 ATGCAT ATGCA^T
NspV 1136 TTCGAA TT^CGAA
PaeR7I 1478 CTCGAG C^TCGAG
PflMI 2671 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2517 CACGTG CAC^GTG
PmlI 2517 CACGTG CAC^GTG
PvuII 2459 CAGCTG CAG^CTG
SexAI 640 ACCAGGT A^CC[AT]GGT
SfuI 1136 TTCGAA TT^CGAA
SpeI 3142 ACTAGT A^CTAGT
SspBI 2513 TGTACA T^GTACA
SspI 1577 AATATT AAT^ATT
StuI 2578 AGGCCT AGG^CCT
StyI 3111 CCTTGG C^C[AT][AT]GG
Van91I 2671 CCACCAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1478 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AlwNI 1322 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
3461 CAGGACCTG
AspI 2416 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3422 GACGCAGTC
BanII 1331 GAGCTC G[GA]GC[CT]^C
2230 GAGCTC
BglII 2466 AGATCT A^GATCT
2472 AGATCT
BsaBI 334 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2467 GATCTAGATC
BsaI 3324 GAGACC GGTCTC(1/5)
2691 GGTCTC
BsmI 1785 GAATGC GAATGC(1/-1)
2024 GAATGC
BspDI 322 ATCGAT AT^CGAT
2203 ATCGAT
BsrDI 2961 CATTGC GCAATG(2/0)
2966 CATTGC
BstXI 161 CCAAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
760 CCATTGTTCTGG
ClaI 322 ATCGAT AT^CGAT
2203 ATCGAT
DraII 3174 GGGTCCT [GA]G^G[ACGT]CC[CT]
3462 AGGACCT
EcoO109I 3174 GGGTCCT [GA]G^G[ACGT]CC[CT]
3462 AGGACCT
MamI 334 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2467 GATCTAGATC
NdeI 176 CATATG CA^TATG
925 CATATG
PflFI 2416 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3422 GACGCAGTC
PpuMI 3174 GGGTCCT [GA]G^G[AT]CC[CT]
3462 AGGACCT
SacI 1331 GAGCTC GAGCT^C
2230 GAGCTC
ScaI 165 AGTACT AGT^ACT
1545 AGTACT
SphI 443 GCATGC GCATG^C
1935 GCATGC
Tth111I 2416 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
3422 GACGCAGTC
XbaI 1074 TCTAGA T^CTAGA
2469 TCTAGA
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI AvaII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI