Restriction map for
pFN21AA0882
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# NO CUT ENZYMES ( 81 enzymes) #
##################################
AatII AclI AcyI AflII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp700I AspEI AviII AvrII
BamHI BbeI BbrPI BfrI BglII
BlnI BsaBI BsaHI BsiEI BsiWI
BspHI BspMI BssHII Bst1107I BstEII
BstPI BstZ17I CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRI EcoT22I FseI FspI
Hin1I HpaI KasI KspI MamI
MluI NarI NdeI NotI NruI
NsiI PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SexAI SfoI SnaBI SpeI
Sse8387I StuI SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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Acc65I 142 GGTACC G^GTACC
AccIII 3737 TCCGGA T^CCGGA
AocI 2296 CCTGAGG CC^T[ACGT]AGG
Asp718I 142 GGTACC G^GTACC
BbvCI 540 CCTCAGC CCTCAGC(-5/-2)
BciVI 2415 GGATAC GTATCC(6/5)
BlpI 2271 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2271 GCTCAGC GC^T[ACGT]AGC
BsaAI 3313 TACGTG [CT]AC^GT[GA]
BseAI 3737 TCCGGA T^CCGGA
BsmBI 1290 CGTCTC CGTCTC(1/5)
BspDI 794 ATCGAT AT^CGAT
BspEI 3737 TCCGGA T^CCGGA
BstAPI 2799 GCACGTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1775 TTCGAA TT^CGAA
BstXI 1910 CCAGAGTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2296 CCTGAGG CC^T[ACGT]AGG
CelII 2271 GCTCAGC GC^T[ACGT]AGC
ClaI 794 ATCGAT AT^CGAT
DraI 421 TTTAAA TTT^AAA
Eco81I 2296 CCTGAGG CC^T[ACGT]AGG
EcoRV 328 GATATC GAT^ATC
HindIII 1853 AAGCTT A^AGCTT
KpnI 142 GGTACC GGTAC^C
MfeI 1064 CAATTG C^AATTG
MroI 3737 TCCGGA T^CCGGA
MunI 1064 CAATTG C^AATTG
NheI 1953 GCTAGC G^CTAGC
NspV 1775 TTCGAA TT^CGAA
PflMI 2403 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 76 CTGCAG CTGCA^G
SfcI 76 CTGCAG C^T[GA][CT]AG
SfiI 3342 GGCCAGCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1775 TTCGAA TT^CGAA
SgrAI 96 CGCCGGCG C[GA]^CCGG[CT]G
SspI 1810 AATATT AAT^ATT
Van91I 2403 CCAGACGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflIII 2895 ACATGT A^C[GA][CT]GT
3113 ACATGT
Alw44I 887 GTGCAC G^TGCAC
1922 GTGCAC
ApaLI 887 GTGCAC G^TGCAC
1922 GTGCAC
AspI 1963 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3556 GACACGGTC
BanI 142 GGTACC G^G[CT][GA]CC
1588 GGTGCC
BbsI 1627 GAAGAC GAAGAC(2/6)
3124 GAAGAC
BcgI 2594 GCATTGACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3200 GCAAGTTGTTCG
BclI 659 TGATCA T^GATCA
2823 TGATCA
BpuAI 1627 GAAGAC GAAGAC(2/6)
3124 GAAGAC
BsaI 718 GAGACC GGTCTC(1/5)
1409 GGTCTC
BsaWI 602 TCCGGT [AT]^CCGG[AT]
3737 TCCGGA
BsmI 633 GAATGC GAATGC(1/-1)
3048 GAATGC
Bsp1407I 2789 TGTACA T^GTACA
2890 TGTACA
BspLU11I 2895 ACATGT A^CATGT
3113 ACATGT
BsrDI 366 CATTGC GCAATG(2/0)
1737 CATTGC
BsrGI 2789 TGTACA T^GTACA
2890 TGTACA
BstYI 1181 GGATCT [GA]^GATC[CT]
1496 GGATCT
DrdI 211 GACTCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1933 GACCAAGGTGTC
FbaI 659 TGATCA T^GATCA
2823 TGATCA
HaeII 169 AGCGCC [GA]GCGC^[CT]
1337 AGCGCC
HgaI 2406 GACGC GACGC(5/10)
1364 GCGTC
HincII 481 GTCAAC GT[CT]^[GA]AC
2083 GTTGAC
HindII 481 GTCAAC GT[CT]^[GA]AC
2083 GTTGAC
MflI 1181 GGATCT [GA]^GATC[CT]
1496 GGATCT
NcoI 1646 CCATGG C^CATGG
2757 CCATGG
PflFI 1963 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3556 GACACGGTC
PpuMI 515 AGGTCCC [GA]G^G[AT]CC[CT]
2762 GGGACCT
PvuII 1975 CAGCTG CAG^CTG
2520 CAGCTG
SacI 1317 GAGCTC GAGCT^C
1567 GAGCTC
SapI 2626 GCTCTTC GCTCTTC(1/4)
3282 GCTCTTC
ScaI 2861 AGTACT AGT^ACT
3683 AGTACT
SmaI 173 CCCGGG CCC^GGG
3419 CCCGGG
SmlI 280 CTTGAG C^T[CT][GA]AG
625 CTTGAG
SspBI 2789 TGTACA T^GTACA
2890 TGTACA
Tth111I 1963 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
3556 GACACGGTC
XhoII 1181 GGATCT [GA]^GATC[CT]
1496 GGATCT
XmaCI 173 CCCGGG C^CCGGG
3419 CCCGGG
XmaI 173 CCCGGG C^CCGGG
3419 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrBI BsrFI BsrI BssKI BssSI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SphI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI