Restriction map for
pFN21AA0882
(Linear) (Six-base) MAPSORT of: KIAA0882.seq Check: 8854 from: 1 to: 3798
KIAA0882.orf
With 182 enzymes: SgfI *
February 28, 2007 11:21 ..
AccI GT'mk_AC
Cuts at: 0 221 611 2303 3798
Size: 221 390 1692 1495
Fragments arranged by size:
1692 1495 390 221
Acc65I G'GTAC_C
Cuts at: 0 142 3798
Size: 142 3656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1805 3798
Size: 1805 1993
AflIII A'CryG_T
Cuts at: 0 2895 3113 3798
Size: 2895 218 685
Fragments arranged by size:
2895 685 218
AleI CACnn'nnGTG
Cuts at: 0 340 1822 3798
Size: 340 1482 1976
Fragments arranged by size:
1976 1482 340
AlwNI CAG_nnn'CTG
Cuts at: 0 1386 2391 2409 2842 3144 3648 3798
Size: 1386 1005 18 433 302 504 150
Fragments arranged by size:
1386 1005 504 433 302 150 18
ApaLI G'TGCA_C
Cuts at: 0 887 1922 3798
Size: 887 1035 1876
Fragments arranged by size:
1876 1035 887
ApoI r'AATT_y
Cuts at: 0 439 2338 2985 3798
Size: 439 1899 647 813
Fragments arranged by size:
1899 813 647 439
AvaI C'yCGr_G
Cuts at: 0 173 1414 3419 3798
Size: 173 1241 2005 379
Fragments arranged by size:
2005 1241 379 173
BanI G'GyrC_C
Cuts at: 0 142 1588 3798
Size: 142 1446 2210
Fragments arranged by size:
2210 1446 142
BanII G_rGCy'C
Cuts at: 0 1321 1571 3335 3798
Size: 1321 250 1764 463
Fragments arranged by size:
1764 1321 463 250
BbsI GAAGACnn'nnnn_
Cuts at: 0 1634 3131 3798
Size: 1634 1497 667
Fragments arranged by size:
1634 1497 667
BbvCI CC'TCA_GC
Cuts at: 0 541 3798
Size: 541 3257
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2583 2617 3189 3223 3798
Size: 2583 34 572 34 575
Fragments arranged by size:
2583 575 572 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2409 3798
Size: 2409 1389
BclI T'GATC_A
Cuts at: 0 659 2823 3798
Size: 659 2164 975
Fragments arranged by size:
2164 975 659
BglI GCCn_nnn'nGGC
Cuts at: 0 98 3266 3349 3798
Size: 98 3168 83 449
Fragments arranged by size:
3168 449 98 83
BlpI GC'TnA_GC
Cuts at: 0 2272 3798
Size: 2272 1526
Bme1580I G_kGCm'C
Cuts at: 0 891 1926 3586 3798
Size: 891 1035 1660 212
Fragments arranged by size:
1660 1035 891 212
BmgBI CAC'GTC
Cuts at: 0 2802 3798
Size: 2802 996
BmrI ACTGGGnnnn_n'
Cuts at: 0 279 1094 2538 3633 3798
Size: 279 815 1444 1095 165
Fragments arranged by size:
1444 1095 815 279 165
BmtI G_CTAG'C
Cuts at: 0 1957 3798
Size: 1957 1841
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1287 2751 2835 3387 3429 3798
Size: 1287 1464 84 552 42 369
Fragments arranged by size:
1464 1287 552 369 84 42
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 191 1071 1281 2431 3008 3202 3424
Size: 150 41 880 210 1150 577 194 222
Cuts at: 3424 3798
Size: 374
Fragments arranged by size:
1150 880 577 374 222 210 194 150
41
Bpu10I CC'TnA_GC
Cuts at: 0 541 2810 3798
Size: 541 2269 988
Fragments arranged by size:
2269 988 541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 646 3798
Size: 301 345 3152
Fragments arranged by size:
3152 345 301
BsaI GGTCTCn'nnnn_
Cuts at: 0 712 1415 3798
Size: 712 703 2383
Fragments arranged by size:
2383 712 703
BsaAI yAC'GTr
Cuts at: 0 3315 3798
Size: 3315 483
BsaWI w'CCGG_w
Cuts at: 0 602 3737 3798
Size: 602 3135 61
Fragments arranged by size:
3135 602 61
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 7 37 1519 1549 2229 2248 2259 2278
Size: 7 30 1482 30 680 19 11 19
Cuts at: 2278 3463 3493 3798
Size: 1185 30 305
Fragments arranged by size:
1482 1185 680 305 30 30 30 19
19 11 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 31 1036 1284 1432 1687 1861 1963 3402
Size: 31 1005 248 148 255 174 102 1439
Cuts at: 3402 3429 3535 3798
Size: 27 106 263
Fragments arranged by size:
1439 1005 263 255 248 174 148 106
102 31 27
BseYI C'CCAG_C
Cuts at: 0 1279 1713 2566 3215 3798
Size: 1279 434 853 649 583
Fragments arranged by size:
1279 853 649 583 434
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 921 1195 1727 2090 2951 3517 3798
Size: 921 274 532 363 861 566 281
Fragments arranged by size:
921 861 566 532 363 281 274
BsiHKAI G_wGCw'C
Cuts at: 0 673 891 1097 1321 1571 1926 3284 3607
Size: 673 218 206 224 250 355 1358 323
Cuts at: 3607 3798
Size: 191
Fragments arranged by size:
1358 673 355 323 250 224 218 206
191
BsmI GAATG_Cn'
Cuts at: 0 639 3054 3798
Size: 639 2415 744
Fragments arranged by size:
2415 744 639
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1296 3798
Size: 1296 2502
Bsp1286I G_dGCh'C
Cuts at: 0 673 891 1097 1321 1571 1926 3284 3335
Size: 673 218 206 224 250 355 1358 51
Cuts at: 3335 3586 3607 3798
Size: 251 21 191
Fragments arranged by size:
1358 673 355 251 250 224 218 206
191 51 21
BspEI T'CCGG_A
Cuts at: 0 3737 3798
Size: 3737 61
BsrBI CCG'CTC
Cuts at: 0 1050 1697 3239 3575 3798
Size: 1050 647 1542 336 223
Fragments arranged by size:
1542 1050 647 336 223
BsrDI GCAATG_nn'
Cuts at: 0 365 1736 3798
Size: 365 1371 2062
Fragments arranged by size:
2062 1371 365
BsrFI r'CCGG_y
Cuts at: 0 97 2555 3339 3508 3798
Size: 97 2458 784 169 290
Fragments arranged by size:
2458 784 290 169 97
BsrGI T'GTAC_A
Cuts at: 0 2789 2890 3798
Size: 2789 101 908
Fragments arranged by size:
2789 908 101
BssSI C'ACGA_G
Cuts at: 0 1314 1958 3517 3798
Size: 1314 644 1559 281
Fragments arranged by size:
1559 1314 644 281
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2805 3798
Size: 2805 993
BstBI TT'CG_AA
Cuts at: 0 1776 3798
Size: 1776 2022
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1917 3798
Size: 1917 1881
BstYI r'GATC_y
Cuts at: 0 1181 1496 3798
Size: 1181 315 2302
Fragments arranged by size:
2302 1181 315
Bsu36I CC'TnA_GG
Cuts at: 0 2297 3798
Size: 2297 1501
BtgI C'CryG_G
Cuts at: 0 1646 1667 2757 3155 3798
Size: 1646 21 1090 398 643
Fragments arranged by size:
1646 1090 643 398 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 116 216 2291 3798
Size: 116 100 2075 1507
Fragments arranged by size:
2075 1507 116 100
BtsI GCAGTG_nn'
Cuts at: 0 2239 3170 3252 3534 3592 3798
Size: 2239 931 82 282 58 206
Fragments arranged by size:
2239 931 282 206 82 58
ClaI AT'CG_AT
Cuts at: 0 795 3798
Size: 795 3003
DraI TTT'AAA
Cuts at: 0 423 3798
Size: 423 3375
DrdI GACnn_nn'nnGTC
Cuts at: 0 217 1939 3798
Size: 217 1722 1859
Fragments arranged by size:
1859 1722 217
EaeI y'GGCC_r
Cuts at: 0 29 924 1436 3341 3576 3710 3764 3785
Size: 29 895 512 1905 235 134 54 21
Cuts at: 3785 3798
Size: 13
Fragments arranged by size:
1905 895 512 235 134 54 29 21
13
EarI CTCTTCn'nnn_
Cuts at: 0 464 2633 2971 3289 3798
Size: 464 2169 338 318 509
Fragments arranged by size:
2169 509 464 338 318
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 134 3798
Size: 134 3664
EcoICRI GAG'CTC
Cuts at: 0 1319 1569 3798
Size: 1319 250 2229
Fragments arranged by size:
2229 1319 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 191 1071 1281 1805 2431 3008 3202
Size: 150 41 880 210 524 626 577 194
Cuts at: 3202 3424 3798
Size: 222 374
Fragments arranged by size:
880 626 577 524 374 222 210 194
150 41
EcoO109I rG'GnC_Cy
Cuts at: 0 516 2763 3431 3798
Size: 516 2247 668 367
Fragments arranged by size:
2247 668 516 367
EcoRV GAT'ATC
Cuts at: 0 330 3798
Size: 330 3468
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1491 3798
Size: 1491 2307
HaeII r_GCGC'y
Cuts at: 0 173 1341 3798
Size: 173 1168 2457
Fragments arranged by size:
2457 1168 173
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1383 1415 1952 1984 3463 3495 3798
Size: 1383 32 537 32 1479 32 303
Fragments arranged by size:
1479 1383 537 303 32 32 32
HincII GTy'rAC
Cuts at: 0 483 2085 3798
Size: 483 1602 1713
Fragments arranged by size:
1713 1602 483
HindIII A'AGCT_T
Cuts at: 0 1853 3798
Size: 1853 1945
KpnI G_GTAC'C
Cuts at: 0 146 3798
Size: 146 3652
MfeI C'AATT_G
Cuts at: 0 1064 3798
Size: 1064 2734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 511 2883 3798
Size: 140 371 2372 915
Fragments arranged by size:
2372 915 371 140
MscI TGG'CCA
Cuts at: 0 31 926 1438 3712 3798
Size: 31 895 512 2274 86
Fragments arranged by size:
2274 895 512 86 31
MslI CAynn'nnrTG
Cuts at: 0 340 754 1822 3739 3798
Size: 340 414 1068 1917 59
Fragments arranged by size:
1917 1068 414 340 59
MspA1I CmG'CkG
Cuts at: 0 81 1977 2522 3798
Size: 81 1896 545 1276
Fragments arranged by size:
1896 1276 545 81
NaeI GCC'GGC
Cuts at: 0 99 3341 3510 3798
Size: 99 3242 169 288
Fragments arranged by size:
3242 288 169 99
NcoI C'CATG_G
Cuts at: 0 1646 2757 3798
Size: 1646 1111 1041
Fragments arranged by size:
1646 1111 1041
NgoMIV G'CCGG_C
Cuts at: 0 97 3339 3508 3798
Size: 97 3242 169 290
Fragments arranged by size:
3242 290 169 97
NheI G'CTAG_C
Cuts at: 0 1953 3798
Size: 1953 1845
NspI r_CATG'y
Cuts at: 0 1506 1560 1890 1992 2648 2756 2899 3117
Size: 1506 54 330 102 656 108 143 218
Cuts at: 3117 3798
Size: 681
Fragments arranged by size:
1506 681 656 330 218 143 108 102
54
PciI A'CATG_T
Cuts at: 0 2895 3113 3798
Size: 2895 218 685
Fragments arranged by size:
2895 685 218
PflMI CCAn_nnn'nTGG
Cuts at: 0 2409 3798
Size: 2409 1389
PfoI T'CCnGG_A
Cuts at: 0 247 1547 2723 3798
Size: 247 1300 1176 1075
Fragments arranged by size:
1300 1176 1075 247
PpuMI rG'GwC_Cy
Cuts at: 0 516 2763 3798
Size: 516 2247 1035
Fragments arranged by size:
2247 1035 516
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3100 3132 3798
Size: 3100 32 666
Fragments arranged by size:
3100 666 32
PstI C_TGCA'G
Cuts at: 0 80 3798
Size: 80 3718
PvuII CAG'CTG
Cuts at: 0 1977 2522 3798
Size: 1977 545 1276
Fragments arranged by size:
1977 1276 545
SacI G_AGCT'C
Cuts at: 0 1321 1571 3798
Size: 1321 250 2227
Fragments arranged by size:
2227 1321 250
SapI GCTCTTCn'nnn_
Cuts at: 0 2633 3289 3798
Size: 2633 656 509
Fragments arranged by size:
2633 656 509
ScaI AGT'ACT
Cuts at: 0 2863 3685 3798
Size: 2863 822 113
Fragments arranged by size:
2863 822 113
SfcI C'TryA_G
Cuts at: 0 76 3798
Size: 76 3722
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3349 3798
Size: 3349 449
SgrAI Cr'CCGG_yG
Cuts at: 0 97 3798
Size: 97 3701
SmaI CCC'GGG
Cuts at: 0 175 3421 3798
Size: 175 3246 377
Fragments arranged by size:
3246 377 175
SmlI C'TyrA_G
Cuts at: 0 280 625 3798
Size: 280 345 3173
Fragments arranged by size:
3173 345 280
SphI G_CATG'C
Cuts at: 0 1560 1890 1992 2756 3798
Size: 1560 330 102 764 1042
Fragments arranged by size:
1560 1042 764 330 102
SspI AAT'ATT
Cuts at: 0 1812 3798
Size: 1812 1986
StyI C'CwwG_G
Cuts at: 0 1646 1935 2638 2757 3375 3467 3798
Size: 1646 289 703 119 618 92 331
Fragments arranged by size:
1646 703 618 331 289 119 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1727 1909 3798
Size: 1727 182 1889
Fragments arranged by size:
1889 1727 182
TatI w'GTAC_w
Cuts at: 0 1268 2789 2861 2890 3683 3798
Size: 1268 1521 72 29 793 115
Fragments arranged by size:
1521 1268 793 115 72 29
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2096 2128 2229 2248 2261 2280 3708 3740
Size: 2096 32 101 19 13 19 1428 32
Cuts at: 3740 3798
Size: 58
Fragments arranged by size:
2096 1428 101 58 32 32 19 19
13
Tth111I GACn'n_nGTC
Cuts at: 0 1966 3559 3798
Size: 1966 1593 239
Fragments arranged by size:
1966 1593 239
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 100 2196 2475 3177 3798
Size: 100 2096 279 702 621
Fragments arranged by size:
2096 702 621 279 100
XmaI C'CCGG_G
Cuts at: 0 173 3419 3798
Size: 173 3246 379
Fragments arranged by size:
3246 379 173
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlwNI ApaLI ApoI
AvaI BanI BanII BbsI BbvCI BcgI BciVI BclI
BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BsrDI
BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtgZI BtsI ClaI DraI DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRV FalI HaeII Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NaeI NcoI NgoMIV NheI NspI PciI PflMI PfoI
PpuMI PsrI PstI PvuII SacI SapI ScaI SfcI
SfiI SgrAI SmaI SmlI SphI SspI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BfrBI BglII BsaBI BsaHI BsiEI BsiWI BspHI
BspMI BssHII BstEII BstZ17I CspCI DraIII EagI EcoNI
EcoRI FseI FspI FspAI HpaI KasI MluI NarI
NdeI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacII
SalI SanDI SbfI SexAI SfoI SgfI SnaBI SpeI
SrfI StuI SwaI XbaI XhoI XmnI ZraI