(Circular) (Six-base) MAPSORT of: pFN21AA0866.seq Check: 3967 from: 1 to: 10621 pFN21AA0866 10621 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2403 4531 5773 7564 2403 Size: 2128 1242 1791 5460 Fragments arranged by size: 5460 2128 1791 1242 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2760 8431 278 Size: 53 83 186 936 1224 5671 2468 Fragments arranged by size: 5671 2468 1224 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2421 6008 7964 1937 Size: 484 3587 1956 4594 Fragments arranged by size: 4594 3587 1956 484 Acc65I G'GTAC_C Cuts at: 7942 7942 Size: 10621 AclI AA'CG_TT Cuts at: 1563 8748 9121 1563 Size: 7185 373 3063 Fragments arranged by size: 7185 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 5354 5561 6263 6881 7556 7577 8684 10068 Size: 3722 207 702 618 675 21 1107 1384 Cuts at: 10068 10336 1632 Size: 268 1917 Fragments arranged by size: 3722 1917 1384 1107 702 675 618 268 207 21 AflII C'TTAA_G Cuts at: 820 1017 4628 820 Size: 197 3611 6813 Fragments arranged by size: 6813 3611 197 AflIII A'CryG_T Cuts at: 1203 2424 6003 9520 10497 1203 Size: 1221 3579 3517 977 1327 Fragments arranged by size: 3579 3517 1327 1221 977 AgeI A'CCGG_T Cuts at: 9452 9452 Size: 10621 AhdI GACnn_n'nnGTC Cuts at: 3112 5935 9350 3112 Size: 2823 3415 4383 Fragments arranged by size: 4383 3415 2823 AleI CACnn'nnGTG Cuts at: 1166 3320 4523 1166 Size: 2154 1203 7264 Fragments arranged by size: 7264 2154 1203 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4130 6168 7727 1184 Size: 2946 2038 1559 4078 Fragments arranged by size: 4078 2946 2038 1559 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2702 2734 3608 3640 4995 5027 2702 Size: 32 874 32 1355 32 8296 Fragments arranged by size: 8296 1355 874 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2587 4441 4721 4762 4861 4909 5161 5689 5794 Size: 1854 280 41 99 48 252 528 105 Cuts at: 5794 5971 6088 6289 8364 9936 2587 Size: 177 117 201 2075 1572 3272 Fragments arranged by size: 3272 2075 1854 1572 528 280 252 201 177 117 105 99 48 41 ApaI G_GGCC'C Cuts at: 5827 6478 6613 5827 Size: 651 135 9835 Fragments arranged by size: 9835 651 135 ApaLI G'TGCA_C Cuts at: 3664 8678 9834 3664 Size: 5014 1156 4451 Fragments arranged by size: 5014 4451 1156 ApoI r'AATT_y Cuts at: 1493 2735 6359 7930 8156 8308 10417 1493 Size: 1242 3624 1571 226 152 2109 1697 Fragments arranged by size: 3624 2109 1697 1571 1242 226 152 AseI AT'TA_AT Cuts at: 160 1032 3165 9175 160 Size: 872 2133 6010 1606 Fragments arranged by size: 6010 2133 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10621 AvaI C'yCGr_G Cuts at: 1870 1943 5826 6471 6509 6750 7127 7373 7884 Size: 73 3883 645 38 241 377 246 511 Cuts at: 7884 7946 1870 Size: 62 4545 Fragments arranged by size: 4545 3883 645 511 377 246 241 73 62 38 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2614 2647 2614 Size: 33 10588 BamHI G'GATC_C Cuts at: 3372 7951 8318 3372 Size: 4579 367 5675 Fragments arranged by size: 5675 4579 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 7942 9397 618 Size: 325 217 627 6155 1455 1842 Fragments arranged by size: 6155 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3567 3917 5247 5508 5628 5827 5838 6478 Size: 2176 350 1330 261 120 199 11 640 Cuts at: 6478 6613 7111 7395 7940 1391 Size: 135 498 284 545 4072 Fragments arranged by size: 4072 2176 1330 640 545 498 350 284 261 199 135 120 11 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3076 4156 4598 4815 5478 6552 6644 Size: 971 1177 1080 442 217 663 1074 92 Cuts at: 6644 6828 928 Size: 184 4721 Fragments arranged by size: 4721 1177 1080 1074 971 663 442 217 184 92 BbvCI CC'TCA_GC Cuts at: 4722 4932 6426 4722 Size: 210 1494 8917 Fragments arranged by size: 8917 1494 210 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2734 2768 8812 8846 1903 Size: 34 797 34 6044 34 3678 Fragments arranged by size: 6044 3678 797 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3303 8513 9723 3303 Size: 5210 1210 4201 Fragments arranged by size: 5210 4201 1210 BclI T'GATC_A Cuts at: 1548 4254 4978 6364 1548 Size: 2706 724 1386 5805 Fragments arranged by size: 5805 2706 1386 724 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2597 4143 4385 Size: 107 122 71 631 605 925 1546 242 Cuts at: 4385 4415 9232 136 Size: 30 4817 1525 Fragments arranged by size: 4817 1546 1525 925 631 605 242 122 107 71 30 BglII A'GATC_T Cuts at: 3396 7014 10616 3396 Size: 3618 3602 3401 Fragments arranged by size: 3618 3602 3401 BlpI GC'TnA_GC Cuts at: 7310 8038 7310 Size: 728 9893 Bme1580I G_kGCm'C Cuts at: 1790 2952 3668 5827 6478 6613 6844 7363 8682 Size: 1162 716 2159 651 135 231 519 1319 Cuts at: 8682 9838 1790 Size: 1156 2573 Fragments arranged by size: 2573 2159 1319 1162 1156 716 651 519 231 135 BmgBI CAC'GTC Cuts at: 1318 2286 3868 5932 6571 1318 Size: 968 1582 2064 639 5368 Fragments arranged by size: 5368 2064 1582 968 639 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2069 2101 8352 Size: 416 53 83 399 24 652 32 6251 Cuts at: 8352 9310 442 Size: 958 1753 Fragments arranged by size: 6251 1753 958 652 416 399 83 53 32 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10621 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3427 3427 Size: 10621 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2565 2699 2892 3506 3573 4112 4784 4817 Size: 1326 134 193 614 67 539 672 33 Cuts at: 4817 4901 4973 5183 5204 5210 5357 5372 5468 Size: 84 72 210 21 6 147 15 96 Cuts at: 5468 5711 5795 5939 6047 6110 6614 6635 6677 Size: 243 84 144 108 63 504 21 42 Cuts at: 6677 6911 6988 7169 7226 7247 7259 7481 7682 Size: 234 77 181 57 21 12 222 201 Cuts at: 7682 7851 9281 1239 Size: 169 1430 2579 Fragments arranged by size: 2579 1430 1326 672 614 539 504 243 234 222 210 201 193 181 169 147 144 134 108 96 84 84 77 72 67 63 57 42 33 21 21 21 15 12 6 Bpu10I CC'TnA_GC Cuts at: 4722 4932 5507 5580 6426 7787 4722 Size: 210 575 73 846 1361 7556 Fragments arranged by size: 7556 1361 846 575 210 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3695 4469 4937 5036 5231 5660 6704 7634 7760 Size: 774 468 99 195 429 1044 930 126 Cuts at: 7760 8746 9611 9909 3695 Size: 986 865 298 4407 Fragments arranged by size: 4407 1044 986 930 865 774 468 429 298 195 126 99 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2783 5424 6611 7796 9284 882 Size: 617 1284 2641 1187 1185 1488 2219 Fragments arranged by size: 2641 2219 1488 1284 1187 1185 617 BsaAI yAC'GTr Cuts at: 493 1204 2352 8412 493 Size: 711 1148 6060 2702 Fragments arranged by size: 6060 2702 1148 711 BsaBI GATnn'nnATC Cuts at: 10615 10615 Size: 10621 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2757 3113 4323 5936 Size: 53 83 186 936 1224 356 1210 1613 Cuts at: 5936 6684 8428 8810 9445 275 Size: 748 1744 382 635 1451 Fragments arranged by size: 1744 1613 1451 1224 1210 936 748 635 382 356 186 83 53 BsaWI w'CCGG_w Cuts at: 2490 4155 7395 9054 9452 9726 9873 10284 2490 Size: 1665 3240 1659 398 274 147 411 2827 Fragments arranged by size: 3240 2827 1665 1659 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2270 2285 2300 2315 2504 Size: 30 165 30 867 15 15 15 189 Cuts at: 2504 2534 2702 2732 3647 3677 4995 5025 5783 Size: 30 168 30 915 30 1318 30 758 Cuts at: 5783 5813 1178 Size: 30 5986 Fragments arranged by size: 5986 1318 915 867 758 189 168 165 30 30 30 30 30 30 30 15 15 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2593 2710 3038 3041 3189 3509 3823 Size: 988 419 117 328 3 148 320 314 Cuts at: 3823 4355 4358 4571 4574 4679 4739 4781 4814 Size: 532 3 213 3 105 60 42 33 Cuts at: 4814 4817 4898 4901 5033 5285 5288 5456 5636 Size: 3 81 3 132 252 3 168 180 Cuts at: 5636 6044 6047 6080 6245 6395 6644 6782 6790 Size: 408 3 33 165 150 249 138 8 Cuts at: 6790 7163 7244 7247 7256 7259 7499 7679 7688 Size: 373 81 3 9 3 240 180 9 Cuts at: 7688 7691 7883 1186 Size: 3 192 3924 Fragments arranged by size: 3924 988 532 419 408 373 328 320 314 252 249 240 213 192 180 180 168 165 150 148 138 132 117 105 81 81 60 42 33 33 9 9 8 3 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2956 3746 3923 3985 4851 5949 6219 7221 7683 Size: 790 177 62 866 1098 270 1002 462 Cuts at: 7683 9824 10320 2956 Size: 2141 496 3257 Fragments arranged by size: 3257 2141 1098 1002 866 790 496 462 270 177 62 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5807 5827 1163 Size: 573 4071 20 5957 Fragments arranged by size: 5957 4071 573 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3578 6243 7276 8071 8832 9860 Size: 275 186 1577 2665 1033 795 761 1028 Cuts at: 9860 1540 Size: 2301 Fragments arranged by size: 2665 2301 1577 1033 1028 795 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3567 3668 3917 5247 5508 5628 5838 7395 8682 Size: 101 249 1330 261 120 210 1557 1287 Cuts at: 8682 8767 9838 3567 Size: 85 1071 4350 Fragments arranged by size: 4350 1557 1330 1287 1071 261 249 210 120 101 85 BsmI GAATG_Cn' Cuts at: 3623 4125 4210 4245 8132 8225 3623 Size: 502 85 35 3887 93 6019 Fragments arranged by size: 6019 3887 502 93 85 35 BsmBI CGTCTCn'nnnn_ Cuts at: 2826 5555 6803 7881 2826 Size: 2729 1248 1078 5566 Fragments arranged by size: 5566 2729 1248 1078 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2952 3567 3668 3917 5247 5508 5628 Size: 399 1162 615 101 249 1330 261 120 Cuts at: 5628 5827 5838 6478 6613 6844 7111 7363 7395 Size: 199 11 640 135 231 267 252 32 Cuts at: 7395 7940 8682 8767 9838 1391 Size: 545 742 85 1071 2174 Fragments arranged by size: 2174 1330 1162 1071 742 640 615 545 399 267 261 252 249 231 199 135 120 101 85 32 11 BspEI T'CCGG_A Cuts at: 7395 10284 7395 Size: 2889 7732 BspHI T'CATG_A Cuts at: 4251 8510 10240 4251 Size: 4259 1730 4632 Fragments arranged by size: 4632 4259 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2403 4531 4683 5773 7480 7564 7976 844 Size: 1559 2128 152 1090 1707 84 412 3489 Fragments arranged by size: 3489 2128 1707 1559 1090 412 152 84 BsrBI CCG'CTC Cuts at: 1394 1823 2326 4603 5395 5821 6037 6514 7378 Size: 429 503 2277 792 426 216 477 864 Cuts at: 7378 8508 1394 Size: 1130 3507 Fragments arranged by size: 3507 2277 1130 864 792 503 477 429 426 216 BsrDI GCAATG_nn' Cuts at: 65 1248 4114 7636 9116 9290 65 Size: 1183 2866 3522 1480 174 1396 Fragments arranged by size: 3522 2866 1480 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4137 9265 9452 1700 Size: 110 2327 5128 187 2869 Fragments arranged by size: 5128 2869 2327 187 110 BsrGI T'GTAC_A Cuts at: 96 2427 3972 96 Size: 2331 1545 6745 Fragments arranged by size: 6745 2331 1545 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10621 BssSI C'ACGA_G Cuts at: 2447 4052 5207 6329 6800 7409 8681 9693 2447 Size: 1605 1155 1122 471 609 1272 1012 3375 Fragments arranged by size: 3375 1605 1272 1155 1122 1012 609 471 BstAPI GCAn_nnn'nTGC Cuts at: 794 2452 4909 5794 794 Size: 1658 2457 885 5621 Fragments arranged by size: 5621 2457 1658 885 BstBI TT'CG_AA Cuts at: 6732 9425 6732 Size: 2693 7928 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10621 BstXI CCAn_nnnn'nTGG Cuts at: 3746 3790 5920 5932 6163 7057 3746 Size: 44 2130 12 231 894 7310 Fragments arranged by size: 7310 2130 894 231 44 12 BstYI r'GATC_y Cuts at: 1972 3185 3372 3396 4191 4962 5340 6739 7014 Size: 1213 187 24 795 771 378 1399 275 Cuts at: 7014 7951 8318 8703 8720 10172 10258 10270 10616 Size: 937 367 385 17 1452 86 12 346 Cuts at: 10616 1972 Size: 1977 Fragments arranged by size: 1977 1452 1399 1213 937 795 771 385 378 367 346 275 187 86 24 17 12 Bsu36I CC'TnA_GG Cuts at: 2318 2318 Size: 10621 BtgI C'CryG_G Cuts at: 513 1065 2004 2592 2991 3000 3636 6162 6675 Size: 552 939 588 399 9 636 2526 513 Cuts at: 6675 7734 7752 513 Size: 1059 18 3382 Fragments arranged by size: 3382 2526 1059 939 636 588 552 513 399 18 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2458 4403 5826 7435 487 Size: 683 56 1232 1945 1423 1609 3673 Fragments arranged by size: 3673 1945 1609 1423 1232 683 56 BtsI GCAGTG_nn' Cuts at: 4508 4532 8137 8931 8951 4508 Size: 24 3605 794 20 6178 Fragments arranged by size: 6178 3605 794 24 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10621 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 10586 DraI TTT'AAA Cuts at: 7925 7925 Size: 10621 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9628 809 Size: 507 8312 1802 Fragments arranged by size: 8312 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2589 3276 3849 Size: 54 1425 50 275 18 759 687 573 Cuts at: 3849 3897 4407 4746 5259 5292 5712 5922 6240 Size: 48 510 339 513 33 420 210 318 Cuts at: 6240 7155 7323 7521 7596 8068 8957 10586 8 Size: 915 168 198 75 472 889 1629 43 Fragments arranged by size: 1629 1425 915 889 759 687 573 513 510 472 420 339 318 275 210 198 168 75 54 50 48 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 6240 8068 1537 Size: 275 4428 1828 4090 Fragments arranged by size: 4428 4090 1828 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2369 2435 3467 4052 5049 5136 5141 5553 Size: 1016 66 1032 585 997 87 5 412 Cuts at: 5553 6171 6182 6400 6477 6645 6767 8551 1353 Size: 618 11 218 77 168 122 1784 3423 Fragments arranged by size: 3423 1784 1032 1016 997 618 585 412 218 168 122 87 77 66 11 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2291 4166 5846 9208 9582 9728 Size: 1064 138 702 1875 1680 3362 374 146 Cuts at: 9728 387 Size: 1280 Fragments arranged by size: 3362 1875 1680 1280 1064 702 374 146 138 EcoICRI GAG'CTC Cuts at: 3565 3915 5245 5506 5626 5836 7393 3565 Size: 350 1330 261 120 210 1557 6793 Fragments arranged by size: 6793 1557 1330 350 261 210 120 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2565 2699 2892 3506 3573 4112 4784 Size: 393 933 134 193 614 67 539 672 Cuts at: 4784 4817 4901 4973 5183 5204 5210 5354 5357 Size: 33 84 72 210 21 6 144 3 Cuts at: 5357 5372 5468 5561 5711 5795 5939 6047 6110 Size: 15 96 93 150 84 144 108 63 Cuts at: 6110 6263 6614 6635 6677 6881 6911 6988 7169 Size: 153 351 21 42 204 30 77 181 Cuts at: 7169 7226 7247 7259 7481 7556 7577 7682 7851 Size: 57 21 12 222 75 21 105 169 Cuts at: 7851 8684 9281 10068 10336 1239 Size: 833 597 787 268 1524 Fragments arranged by size: 1524 933 833 787 672 614 597 539 393 351 268 222 210 204 193 181 169 153 150 144 144 134 108 105 96 93 84 84 77 75 72 67 63 57 42 33 30 21 21 21 21 15 12 6 3 EcoNI CCTnn'n_nnAGG Cuts at: 3488 3860 4340 4679 4799 5375 5438 5735 6482 Size: 372 480 339 120 576 63 297 747 Cuts at: 6482 7130 3488 Size: 648 6979 Fragments arranged by size: 6979 747 648 576 480 372 339 297 120 63 EcoO109I rG'GnC_Cy Cuts at: 2116 5380 5452 5548 5728 6457 6475 6610 6877 Size: 3264 72 96 180 729 18 135 267 Cuts at: 6877 7087 2116 Size: 210 5650 Fragments arranged by size: 5650 3264 729 267 210 180 135 96 72 18 EcoRI G'AATT_C Cuts at: 7930 8308 7930 Size: 378 10243 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2062 2152 7582 2062 Size: 90 5430 5101 Fragments arranged by size: 5430 5101 90 FspI TGC'GCA Cuts at: 9127 9127 Size: 10621 HaeII r_GCGC'y Cuts at: 1678 3298 3930 6097 6719 7201 7460 9768 1678 Size: 1620 632 2167 622 482 259 2308 2531 Fragments arranged by size: 2531 2308 2167 1620 632 622 482 259 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2443 2475 2504 2536 2705 Size: 32 135 32 871 32 29 32 169 Cuts at: 2705 2737 3020 3052 3098 3130 3177 3209 3381 Size: 32 283 32 46 32 47 32 172 Cuts at: 3381 3413 4046 4078 5372 5404 5552 5584 5783 Size: 32 633 32 1294 32 148 32 199 Cuts at: 5783 5815 5921 5953 6999 7031 7664 7696 9262 Size: 32 106 32 1046 32 633 32 1566 Cuts at: 9262 9294 9336 9368 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1566 1294 1046 871 633 633 283 199 172 169 148 135 106 47 46 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 29 HincII GTy'rAC Cuts at: 669 1657 1938 2380 2422 6706 7965 8211 669 Size: 988 281 442 42 4284 1259 246 3079 Fragments arranged by size: 4284 3079 1259 988 442 281 246 42 HindIII A'AGCT_T Cuts at: 748 748 Size: 10621 HpaI GTT'AAC Cuts at: 8211 8211 Size: 10621 KpnI G_GTAC'C Cuts at: 7946 7946 Size: 10621 MfeI C'AATT_G Cuts at: 8220 8220 Size: 10621 MluI A'CGCG_T Cuts at: 10497 10497 Size: 10621 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3861 6150 6360 9735 9919 1305 Size: 2556 2289 210 3375 184 2007 Fragments arranged by size: 3375 2556 2289 2007 210 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3278 3899 4409 5261 5294 Size: 54 1425 343 1446 621 510 852 33 Cuts at: 5294 5924 7157 7325 10 Size: 630 1233 168 3306 Fragments arranged by size: 3306 1446 1425 1233 852 630 621 510 343 168 54 33 MslI CAynn'nnrTG Cuts at: 518 1166 3320 4250 4454 4523 5885 6804 8579 Size: 648 2154 930 204 69 1362 919 1775 Cuts at: 8579 8938 9097 518 Size: 359 159 2042 Fragments arranged by size: 2154 2042 1775 1362 930 919 648 359 204 159 69 MspA1I CmG'CkG Cuts at: 1606 3134 3306 3424 3799 3970 3985 4099 4441 Size: 1528 172 118 375 171 15 114 342 Cuts at: 4441 4657 4690 4879 5257 6025 6058 6322 6646 Size: 216 33 189 378 768 33 264 324 Cuts at: 6646 6851 7100 7225 7660 7717 8037 8714 9862 Size: 205 249 125 435 57 320 677 1148 Cuts at: 9862 10107 1606 Size: 245 2120 Fragments arranged by size: 2120 1528 1148 768 677 435 378 375 342 324 320 264 249 245 216 205 189 172 171 125 118 114 57 33 33 15 NaeI GCC'GGC Cuts at: 4139 4139 Size: 10621 NcoI C'CATG_G Cuts at: 513 1065 2004 3000 7752 513 Size: 552 939 996 4752 3382 Fragments arranged by size: 4752 3382 996 939 552 NdeI CA'TA_TG Cuts at: 387 3289 387 Size: 2902 7719 NgoMIV G'CCGG_C Cuts at: 4137 4137 Size: 10621 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10621 NotI GC'GGCC_GC Cuts at: 6240 8068 6240 Size: 1828 8793 NspI r_CATG'y Cuts at: 1132 1229 2428 2500 3952 6712 7979 9524 1132 Size: 97 1199 72 1452 2760 1267 1545 2229 Fragments arranged by size: 2760 2229 1545 1452 1267 1199 97 72 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10621 PciI A'CATG_T Cuts at: 2424 9520 2424 Size: 7096 3525 PflMI CCAn_nnn'nTGG Cuts at: 1108 3157 6592 7414 1108 Size: 2049 3435 822 4315 Fragments arranged by size: 4315 3435 2049 822 PfoI T'CCnGG_A Cuts at: 2982 3375 3483 4167 5343 5373 5586 5955 7185 Size: 393 108 684 1176 30 213 369 1230 Cuts at: 7185 2982 Size: 6418 Fragments arranged by size: 6418 1230 1176 684 393 369 213 108 30 PmeI GTTT'AAAC Cuts at: 7925 7925 Size: 10621 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2702 2734 4995 5027 8690 8722 10203 10235 2702 Size: 32 2261 32 3663 32 1481 32 3088 Fragments arranged by size: 3663 3088 2261 1481 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2116 5380 5728 6877 7087 2116 Size: 3264 348 1149 210 5650 Fragments arranged by size: 5650 3264 1149 348 210 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10621 PsiI TTA'TAA Cuts at: 8191 8191 Size: 10621 PspOMI G'GGCC_C Cuts at: 5823 6474 6609 5823 Size: 651 135 9835 Fragments arranged by size: 9835 651 135 PspXI vC'TCGA_Gb Cuts at: 6509 7373 6509 Size: 864 9757 PstI C_TGCA'G Cuts at: 830 3069 3456 4542 4590 4695 5283 5799 5862 Size: 2239 387 1086 48 105 588 516 63 Cuts at: 5862 6147 6789 6856 6957 7575 7716 7973 830 Size: 285 642 67 101 618 141 257 3478 Fragments arranged by size: 3478 2239 1086 642 618 588 516 387 285 257 141 105 101 67 63 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10621 PvuII CAG'CTG Cuts at: 3134 3424 3970 3985 4099 4441 4657 4690 4879 Size: 290 546 15 114 342 216 33 189 Cuts at: 4879 5257 6025 6058 6646 6851 7225 7660 3134 Size: 378 768 33 588 205 374 435 6095 Fragments arranged by size: 6095 768 588 546 435 378 374 342 290 216 205 189 114 33 33 15 SacI G_AGCT'C Cuts at: 3567 3917 5247 5508 5628 5838 7395 3567 Size: 350 1330 261 120 210 1557 6793 Fragments arranged by size: 6793 1557 1330 350 261 210 120 SalI G'TCGA_C Cuts at: 1936 2420 7963 1936 Size: 484 5543 4594 Fragments arranged by size: 5543 4594 484 SanDI GG'GwC_CC Cuts at: 2116 2116 Size: 10621 SapI GCTCTTCn'nnn_ Cuts at: 3467 5141 6645 3467 Size: 1674 1504 7443 Fragments arranged by size: 7443 1674 1504 SbfI CC_TGCA'GG Cuts at: 7973 7973 Size: 10621 ScaI AGT'ACT Cuts at: 8869 10581 8869 Size: 1712 8909 SexAI A'CCwGG_T Cuts at: 6297 6549 6297 Size: 252 10369 SfcI C'TryA_G Cuts at: 826 1045 3065 3264 3452 3548 4538 4586 4679 Size: 219 2020 199 188 96 990 48 93 Cuts at: 4679 4691 4826 5279 5795 5858 6143 6785 6852 Size: 12 135 453 516 63 285 642 67 Cuts at: 6852 6953 7571 7712 7969 9104 9785 9976 826 Size: 101 618 141 257 1135 681 191 1471 Fragments arranged by size: 2020 1471 1135 990 681 642 618 516 453 285 257 219 199 191 188 141 135 101 96 93 67 63 48 12 SfiI GGCCn_nnn'nGGCC Cuts at: 2597 2597 Size: 10621 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10621 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10621 SmaI CCC'GGG Cuts at: 1872 5828 6752 7948 1872 Size: 3956 924 1196 4545 Fragments arranged by size: 4545 3956 1196 924 SmlI C'TyrA_G Cuts at: 820 1017 1943 3710 4484 4628 4916 5015 5246 Size: 197 926 1767 774 144 288 99 231 Cuts at: 5246 5639 6509 6719 7127 7373 7649 7775 7884 Size: 393 870 210 408 246 276 126 109 Cuts at: 7884 8725 9626 9888 820 Size: 841 901 262 1553 Fragments arranged by size: 1767 1553 926 901 870 841 774 408 393 288 276 262 246 231 210 197 144 126 109 99 SnaBI TAC'GTA Cuts at: 493 2352 8412 493 Size: 1859 6060 2702 Fragments arranged by size: 6060 2702 1859 SpeI A'CTAG_T Cuts at: 152 152 Size: 10621 SphI G_CATG'C Cuts at: 1132 2500 7979 1132 Size: 1368 5479 3774 Fragments arranged by size: 5479 3774 1368 SrfI GCCC'GGGC Cuts at: 5828 5828 Size: 10621 SspI AAT'ATT Cuts at: 5 52 8545 5 Size: 47 8493 2081 Fragments arranged by size: 8493 2081 47 StuI AGG'CCT Cuts at: 1517 4241 6488 1517 Size: 2724 2247 5650 Fragments arranged by size: 5650 2724 2247 StyI C'CwwG_G Cuts at: 513 1065 2004 2259 2877 3000 3279 4572 4596 Size: 552 939 255 618 123 279 1293 24 Cuts at: 4596 5658 5925 6453 6489 6561 7752 7908 8060 Size: 1062 267 528 36 72 1191 156 152 Cuts at: 8060 513 Size: 3074 Fragments arranged by size: 3074 1293 1191 1062 939 618 552 528 279 267 255 156 152 123 72 36 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2263 3049 3592 4676 5978 6233 8648 Size: 1255 683 786 543 1084 1302 255 2415 Cuts at: 8648 8665 8818 9003 9448 325 Size: 17 153 185 445 1498 Fragments arranged by size: 2415 1498 1302 1255 1084 786 683 543 445 255 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2427 3972 7605 Size: 274 80 33 51 1443 450 1545 3633 Cuts at: 7605 8867 10579 96 Size: 1262 1712 138 Fragments arranged by size: 3633 1712 1545 1443 1262 450 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2270 2302 3153 3185 3647 3679 10581 10613 2270 Size: 32 851 32 462 32 6902 32 2278 Fragments arranged by size: 6902 2278 851 462 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5566 5846 6551 7277 1316 Size: 4250 280 705 726 4660 Fragments arranged by size: 4660 4250 726 705 280 XbaI T'CTAG_A Cuts at: 7820 7957 7820 Size: 137 10484 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3007 3046 3481 4222 5749 6085 6568 6610 6694 Size: 39 435 741 1527 336 483 42 84 Cuts at: 6694 7456 3007 Size: 762 6172 Fragments arranged by size: 6172 1527 762 741 483 435 336 84 42 39 XhoI C'TCGA_G Cuts at: 1943 6509 7127 7373 7884 1943 Size: 4566 618 246 511 4680 Fragments arranged by size: 4680 4566 618 511 246 XmaI C'CCGG_G Cuts at: 1870 5826 6750 7946 1870 Size: 3956 924 1196 4545 Fragments arranged by size: 4545 3956 1196 924 XmnI GAAnn'nnTTC Cuts at: 3413 4125 6191 8750 3413 Size: 712 2066 2559 5284 Fragments arranged by size: 5284 2559 2066 712 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2758 8429 276 Size: 53 83 186 936 1224 5671 2468 Fragments arranged by size: 5671 2468 1224 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacII SfoI SwaI