(Circular) (Six-base) MAPSORT of: pFN21AA0837.seq Check: 7472 from: 1 to: 6871 pFN21AA0837 6871 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4681 278 Size: 53 83 186 936 3145 2468 Fragments arranged by size: 3145 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4214 1937 Size: 2277 4594 Acc65I G'GTAC_C Cuts at: 4192 4192 Size: 6871 AclI AA'CG_TT Cuts at: 1563 4998 5371 1563 Size: 3435 373 3063 Fragments arranged by size: 3435 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3196 3915 3989 4934 6318 6586 1632 Size: 1564 719 74 945 1384 268 1917 Fragments arranged by size: 1917 1564 1384 945 719 268 74 AfeI AGC'GCT Cuts at: 3239 3239 Size: 6871 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6674 AflIII A'CryG_T Cuts at: 1203 3051 4062 5770 6747 1203 Size: 1848 1011 1708 977 1327 Fragments arranged by size: 1848 1708 1327 1011 977 AgeI A'CCGG_T Cuts at: 5702 5702 Size: 6871 AhdI GACnn_n'nnGTC Cuts at: 5600 5600 Size: 6871 AleI CACnn'nnGTG Cuts at: 1166 2168 1166 Size: 1002 5869 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3899 3967 1184 Size: 2715 68 4088 Fragments arranged by size: 4088 2715 68 AlwNI CAG_nnn'CTG Cuts at: 2440 4614 6186 2440 Size: 2174 1572 3125 Fragments arranged by size: 3125 2174 1572 ApaI G_GGCC'C Cuts at: 2400 2627 3916 2400 Size: 227 1289 5355 Fragments arranged by size: 5355 1289 227 ApaLI G'TGCA_C Cuts at: 3466 4928 6084 3466 Size: 1462 1156 4253 Fragments arranged by size: 4253 1462 1156 ApoI r'AATT_y Cuts at: 1493 2471 4180 4406 4558 6667 1493 Size: 978 1709 226 152 2109 1697 Fragments arranged by size: 2109 1709 1697 978 226 152 AseI AT'TA_AT Cuts at: 160 1032 5425 160 Size: 872 4393 1606 Fragments arranged by size: 4393 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6871 AvaI C'yCGr_G Cuts at: 1870 1943 3788 4196 1870 Size: 73 1845 408 4545 Fragments arranged by size: 4545 1845 408 73 BamHI G'GATC_C Cuts at: 4201 4568 4201 Size: 367 6504 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2180 3432 3523 4192 5647 Size: 325 217 627 393 1252 91 669 1455 Cuts at: 5647 618 Size: 1842 Fragments arranged by size: 1842 1455 1252 669 627 393 325 217 91 BanII G_rGCy'C Cuts at: 1391 2036 2400 2627 3916 4190 1391 Size: 645 364 227 1289 274 4072 Fragments arranged by size: 4072 1289 645 364 274 227 BbeI G_GCGC'C Cuts at: 3527 3527 Size: 6871 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5900 BbvCI CC'TCA_GC Cuts at: 2316 2316 Size: 6871 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5062 5096 1903 Size: 34 50 34 3041 34 3678 Fragments arranged by size: 3678 3041 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2097 4763 5973 2097 Size: 2666 1210 2995 Fragments arranged by size: 2995 2666 1210 BclI T'GATC_A Cuts at: 1548 2518 2748 1548 Size: 970 230 5671 Fragments arranged by size: 5671 970 230 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2465 5482 136 Size: 107 122 71 631 605 793 3017 1525 Fragments arranged by size: 3017 1525 793 631 605 122 107 71 BglII A'GATC_T Cuts at: 3207 3946 6866 3207 Size: 739 2920 3212 Fragments arranged by size: 3212 2920 739 BlpI GC'TnA_GC Cuts at: 2378 4288 2378 Size: 1910 4961 Bme1580I G_kGCm'C Cuts at: 1790 2185 2290 2400 2627 2871 3470 3516 3916 Size: 395 105 110 227 244 599 46 400 Cuts at: 3916 4932 6088 1790 Size: 1016 1156 2573 Fragments arranged by size: 2573 1156 1016 599 400 395 244 227 110 105 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6871 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3586 4602 5560 Size: 416 53 83 399 24 2169 1016 958 Cuts at: 5560 442 Size: 1753 Fragments arranged by size: 2169 1753 1016 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6871 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4174 4174 Size: 6871 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2477 2718 3269 3399 3714 5531 1239 Size: 854 384 241 551 130 315 1817 2579 Fragments arranged by size: 2579 1817 854 551 384 315 241 130 Bpu10I CC'TnA_GC Cuts at: 2118 2316 2421 3768 4116 2118 Size: 198 105 1347 348 4873 Fragments arranged by size: 4873 1347 348 198 105 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2726 4996 5861 6159 2726 Size: 2270 865 298 3438 Fragments arranged by size: 3438 2270 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4105 5534 882 Size: 617 2606 1429 2219 Fragments arranged by size: 2606 2219 1429 617 BsaAI yAC'GTr Cuts at: 493 1204 3517 4662 493 Size: 711 2313 1145 2702 Fragments arranged by size: 2702 2313 1145 711 BsaBI GATnn'nnATC Cuts at: 6865 6865 Size: 6871 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3524 4678 5060 5695 Size: 53 83 186 936 1991 1154 382 635 Cuts at: 5695 275 Size: 1451 Fragments arranged by size: 1991 1451 1154 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2483 3192 3282 3813 5304 5702 5976 6123 6534 Size: 709 90 531 1491 398 274 147 411 Cuts at: 6534 2483 Size: 2820 Fragments arranged by size: 2820 1491 709 531 411 398 274 147 90 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3084 3114 3765 3795 4148 Size: 30 165 30 1681 30 651 30 353 Cuts at: 4148 4178 1178 Size: 30 3871 Fragments arranged by size: 3871 1681 651 353 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6871 BseYI C'CCAG_C Cuts at: 3388 6074 6570 3388 Size: 2686 496 3689 Fragments arranged by size: 3689 2686 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3937 1163 Size: 573 2201 4097 Fragments arranged by size: 4097 2201 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4321 5082 6110 1540 Size: 275 186 2320 761 1028 2301 Fragments arranged by size: 2320 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3027 3470 4932 5017 6088 3027 Size: 443 1462 85 1071 3810 Fragments arranged by size: 3810 1462 1071 443 85 BsmI GAATG_Cn' Cuts at: 4382 4475 4382 Size: 93 6778 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2185 2290 2400 2627 2871 3027 Size: 399 246 149 105 110 227 244 156 Cuts at: 3027 3470 3516 3916 4190 4932 5017 6088 1391 Size: 443 46 400 274 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 400 399 274 246 244 227 156 149 110 105 85 46 BspEI T'CCGG_A Cuts at: 2483 3282 3813 6534 2483 Size: 799 531 2721 2820 Fragments arranged by size: 2820 2721 799 531 BspHI T'CATG_A Cuts at: 4760 6490 4760 Size: 1730 5141 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4024 4226 844 Size: 3180 202 3489 Fragments arranged by size: 3489 3180 202 BsrBI CCG'CTC Cuts at: 1394 1823 2608 4758 1394 Size: 429 785 2150 3507 Fragments arranged by size: 3507 2150 785 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5366 5540 65 Size: 1183 4118 174 1396 Fragments arranged by size: 4118 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2217 3715 5515 5702 1700 Size: 110 407 1498 1800 187 2869 Fragments arranged by size: 2869 1800 1498 407 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6871 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6871 BssSI C'ACGA_G Cuts at: 2021 2168 4931 5943 2021 Size: 147 2763 1012 2949 Fragments arranged by size: 2949 2763 1012 147 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6871 BstBI TT'CG_AA Cuts at: 2769 5675 2769 Size: 2906 3965 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6871 BstXI CCAn_nnnn'nTGG Cuts at: 2599 2599 Size: 6871 BstYI r'GATC_y Cuts at: 1972 2094 3207 3643 3946 3964 4201 4568 4953 Size: 122 1113 436 303 18 237 367 385 Cuts at: 4953 4970 6422 6508 6520 6866 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1113 436 385 367 346 303 237 122 86 18 17 12 Bsu36I CC'TnA_GG Cuts at: 2099 3508 2099 Size: 1409 5462 BtgI C'CryG_G Cuts at: 513 1065 2371 2592 2829 2947 3088 3844 4167 Size: 552 1306 221 237 118 141 756 323 Cuts at: 4167 513 Size: 3217 Fragments arranged by size: 3217 1306 756 552 323 237 221 141 118 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2951 3690 487 Size: 683 56 1725 739 3668 Fragments arranged by size: 3668 1725 739 683 56 BtsI GCAGTG_nn' Cuts at: 4387 5181 5201 4387 Size: 794 20 6057 Fragments arranged by size: 6057 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6871 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6836 DraI TTT'AAA Cuts at: 3760 4175 3760 Size: 415 6456 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5878 809 Size: 507 4562 1802 Fragments arranged by size: 4562 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2157 2219 2457 Size: 54 1425 50 275 18 327 62 238 Cuts at: 2457 2549 2842 3454 4318 5207 6836 8 Size: 92 293 612 864 889 1629 43 Fragments arranged by size: 1629 1425 889 864 612 327 293 275 238 92 62 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4318 1537 Size: 275 2506 4090 Fragments arranged by size: 4090 2506 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3329 3914 4143 4801 1353 Size: 1976 585 229 658 3423 Fragments arranged by size: 3423 1976 658 585 229 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2357 3113 5458 5832 5978 387 Size: 1064 138 768 756 2345 374 146 1280 Fragments arranged by size: 2345 1280 1064 768 756 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2477 2718 3196 3269 3399 3714 Size: 393 461 384 241 478 73 130 315 Cuts at: 3714 3915 3989 4934 5531 6318 6586 1239 Size: 201 74 945 597 787 268 1524 Fragments arranged by size: 1524 945 787 597 478 461 393 384 315 268 241 201 130 74 73 EcoNI CCTnn'n_nnAGG Cuts at: 2121 2121 Size: 6871 EcoO109I rG'GnC_Cy Cuts at: 2397 2624 3664 2397 Size: 227 1040 5604 Fragments arranged by size: 5604 1040 227 EcoRI G'AATT_C Cuts at: 4180 4558 4180 Size: 378 6493 EcoRV GAT'ATC Cuts at: 3121 3121 Size: 6871 FspI TGC'GCA Cuts at: 5377 5377 Size: 6871 HaeII r_GCGC'y Cuts at: 1678 3241 3527 6018 1678 Size: 1563 286 2491 2531 Fragments arranged by size: 2531 2491 1563 286 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2873 2905 3491 3523 4052 Size: 32 135 32 1301 32 586 32 529 Cuts at: 4052 4084 5512 5544 5586 5618 1373 Size: 32 1428 32 42 32 2626 Fragments arranged by size: 2626 1428 1301 586 529 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4215 4461 669 Size: 988 281 2277 246 3079 Fragments arranged by size: 3079 2277 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6871 HpaI GTT'AAC Cuts at: 4461 4461 Size: 6871 KasI G'GCGC_C Cuts at: 3523 3523 Size: 6871 KpnI G_GTAC'C Cuts at: 4196 4196 Size: 6871 MfeI C'AATT_G Cuts at: 2500 4470 2500 Size: 1970 4901 MluI A'CGCG_T Cuts at: 6747 6747 Size: 6871 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5985 6169 1305 Size: 4680 184 2007 Fragments arranged by size: 4680 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2844 3456 10 Size: 54 1425 343 1012 612 3425 Fragments arranged by size: 3425 1425 1012 612 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2334 2597 2756 3881 4829 5188 Size: 648 1002 166 263 159 1125 948 359 Cuts at: 5188 5347 518 Size: 159 2042 Fragments arranged by size: 2042 1125 1002 948 648 359 263 166 159 159 MspA1I CmG'CkG Cuts at: 1606 2657 3473 4287 4964 6112 6357 1606 Size: 1051 816 814 677 1148 245 2120 Fragments arranged by size: 2120 1148 1051 816 814 677 245 NaeI GCC'GGC Cuts at: 3717 3717 Size: 6871 NarI GG'CG_CC Cuts at: 3524 3524 Size: 6871 NcoI C'CATG_G Cuts at: 513 1065 2592 2947 3844 4167 513 Size: 552 1527 355 897 323 3217 Fragments arranged by size: 3217 1527 897 552 355 323 NdeI CA'TA_TG Cuts at: 387 3940 387 Size: 3553 3318 NgoMIV G'CCGG_C Cuts at: 3715 3715 Size: 6871 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6871 NotI GC'GGCC_GC Cuts at: 4318 4318 Size: 6871 NspI r_CATG'y Cuts at: 1132 1229 2514 2717 3055 4066 4229 5774 1132 Size: 97 1285 203 338 1011 163 1545 2229 Fragments arranged by size: 2229 1545 1285 1011 338 203 163 97 PasI CC'CwG_GG Cuts at: 1348 2620 2627 1348 Size: 1272 7 5592 Fragments arranged by size: 5592 1272 7 PciI A'CATG_T Cuts at: 3051 4062 5770 3051 Size: 1011 1708 4152 Fragments arranged by size: 4152 1708 1011 PflMI CCAn_nnn'nTGG Cuts at: 1108 2455 3826 1108 Size: 1347 1371 4153 Fragments arranged by size: 4153 1371 1347 PfoI T'CCnGG_A Cuts at: 3246 3246 Size: 6871 PmeI GTTT'AAAC Cuts at: 4175 4175 Size: 6871 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4940 4972 6453 6485 4940 Size: 32 1481 32 5326 Fragments arranged by size: 5326 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6871 PsiI TTA'TAA Cuts at: 4441 4441 Size: 6871 PspOMI G'GGCC_C Cuts at: 2396 2623 3912 2396 Size: 227 1289 5355 Fragments arranged by size: 5355 1289 227 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2348 2380 3182 3214 2348 Size: 32 802 32 6005 Fragments arranged by size: 6005 802 32 32 PstI C_TGCA'G Cuts at: 830 4223 830 Size: 3393 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6871 PvuII CAG'CTG Cuts at: 3473 3473 Size: 6871 SalI G'TCGA_C Cuts at: 1936 4213 1936 Size: 2277 4594 SapI GCTCTTCn'nnn_ Cuts at: 4143 4143 Size: 6871 SbfI CC_TGCA'GG Cuts at: 4223 4223 Size: 6871 ScaI AGT'ACT Cuts at: 4130 5119 6831 4130 Size: 989 1712 4170 Fragments arranged by size: 4170 1712 989 SexAI A'CCwGG_T Cuts at: 2358 2358 Size: 6871 SfcI C'TryA_G Cuts at: 826 1045 4219 5354 6035 6226 826 Size: 219 3174 1135 681 191 1471 Fragments arranged by size: 3174 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 3525 3525 Size: 6871 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6871 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6871 SmaI CCC'GGG Cuts at: 1872 4198 1872 Size: 2326 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2741 4975 5876 6138 820 Size: 197 926 798 2234 901 262 1553 Fragments arranged by size: 2234 1553 926 901 798 262 197 SnaBI TAC'GTA Cuts at: 493 4662 493 Size: 4169 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6871 SphI G_CATG'C Cuts at: 1132 2514 4229 1132 Size: 1382 1715 3774 Fragments arranged by size: 3774 1715 1382 SspI AAT'ATT Cuts at: 5 52 4795 5 Size: 47 4743 2081 Fragments arranged by size: 4743 2081 47 StuI AGG'CCT Cuts at: 1517 2228 2739 3863 1517 Size: 711 511 1124 4525 Fragments arranged by size: 4525 1124 711 511 StyI C'CwwG_G Cuts at: 513 1065 2592 2947 3844 4167 4310 513 Size: 552 1527 355 897 323 143 3074 Fragments arranged by size: 3074 1527 897 552 355 323 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2168 2960 3345 4898 4915 5068 5253 Size: 1255 588 792 385 1553 17 153 185 Cuts at: 5253 5698 325 Size: 445 1498 Fragments arranged by size: 1553 1498 1255 792 588 445 385 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4128 5117 6829 Size: 274 80 33 51 1443 2151 989 1712 Cuts at: 6829 96 Size: 138 Fragments arranged by size: 2151 1712 1443 989 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2079 2111 3606 3638 6831 6863 2079 Size: 32 1495 32 3193 32 2087 Fragments arranged by size: 3193 2087 1495 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6871 XbaI T'CTAG_A Cuts at: 4207 4207 Size: 6871 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2827 3169 3237 2827 Size: 342 68 6461 Fragments arranged by size: 6461 342 68 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6871 XmaI C'CCGG_G Cuts at: 1870 4196 1870 Size: 2326 4545 XmnI GAAnn'nnTTC Cuts at: 5000 5000 Size: 6871 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4679 276 Size: 53 83 186 936 3145 2468 Fragments arranged by size: 3145 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI RsrII SacI SacII SanDI SfiI SrfI SwaI