(Circular) (Six-base) MAPSORT of: pFN21AA0829.seq Check: 8876 from: 1 to: 8395 pFN21AA0829 8395 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2416 6205 278 Size: 53 83 186 936 880 3789 2468 Fragments arranged by size: 3789 2468 936 880 186 83 53 AccI GT'mk_AC Cuts at: 1937 2542 4493 5396 5738 1937 Size: 605 1951 903 342 4594 Fragments arranged by size: 4594 1951 903 605 342 Acc65I G'GTAC_C Cuts at: 5716 5716 Size: 8395 AclI AA'CG_TT Cuts at: 1563 6522 6895 1563 Size: 4959 373 3063 Fragments arranged by size: 4959 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3272 3636 3824 6458 7842 8110 1632 Size: 1640 364 188 2634 1384 268 1917 Fragments arranged by size: 2634 1917 1640 1384 364 268 188 AflII C'TTAA_G Cuts at: 820 1017 4555 4913 820 Size: 197 3538 358 4302 Fragments arranged by size: 4302 3538 358 197 AflIII A'CryG_T Cuts at: 1203 3372 5459 7294 8271 1203 Size: 2169 2087 1835 977 1327 Fragments arranged by size: 2169 2087 1835 1327 977 AgeI A'CCGG_T Cuts at: 7226 7226 Size: 8395 AhdI GACnn_n'nnGTC Cuts at: 7124 7124 Size: 8395 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8395 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2303 1184 Size: 1119 7276 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3942 3974 3942 Size: 32 8363 AlwNI CAG_nnn'CTG Cuts at: 6138 7710 6138 Size: 1572 6823 ApaI G_GGCC'C Cuts at: 3409 3409 Size: 8395 ApaLI G'TGCA_C Cuts at: 6452 7608 6452 Size: 1156 7239 ApoI r'AATT_y Cuts at: 1493 2200 2789 3113 3478 4973 5315 5594 5704 Size: 707 589 324 365 1495 342 279 110 Cuts at: 5704 5930 6082 8191 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1495 707 589 365 342 324 279 226 152 110 AseI AT'TA_AT Cuts at: 160 1032 5123 6949 160 Size: 872 4091 1826 1606 Fragments arranged by size: 4091 1826 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8395 AvaI C'yCGr_G Cuts at: 1870 1943 4425 5720 1870 Size: 73 2482 1295 4545 Fragments arranged by size: 4545 2482 1295 73 BamHI G'GATC_C Cuts at: 4199 5725 6092 4199 Size: 1526 367 6502 Fragments arranged by size: 6502 1526 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5716 7171 618 Size: 325 217 627 3929 1455 1842 Fragments arranged by size: 3929 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3409 4095 4258 5714 1391 Size: 2018 686 163 1456 4072 Fragments arranged by size: 4072 2018 1456 686 163 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3356 3532 5058 928 Size: 971 1457 176 1526 4265 Fragments arranged by size: 4265 1526 1457 971 176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6586 6620 1903 Size: 34 4649 34 3678 Fragments arranged by size: 4649 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4310 5134 5657 6287 7497 4310 Size: 824 523 630 1210 5208 Fragments arranged by size: 5208 1210 824 630 523 BclI T'GATC_A Cuts at: 1548 2998 3748 1548 Size: 1450 750 6195 Fragments arranged by size: 6195 1450 750 BfrBI ATG'CAT Cuts at: 4123 4640 4123 Size: 517 7878 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 7006 136 Size: 107 122 71 631 605 5334 1525 Fragments arranged by size: 5334 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2641 3703 8390 2641 Size: 1062 4687 2646 Fragments arranged by size: 4687 2646 1062 BlpI GC'TnA_GC Cuts at: 5812 5812 Size: 8395 Bme1580I G_kGCm'C Cuts at: 1790 3409 6456 7612 1790 Size: 1619 3047 1156 2573 Fragments arranged by size: 3047 2573 1619 1156 BmgBI CAC'GTC Cuts at: 1318 3056 1318 Size: 1738 6657 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2545 4016 6126 Size: 416 53 83 399 24 1128 1471 2110 Cuts at: 6126 7084 442 Size: 958 1753 Fragments arranged by size: 2110 1753 1471 1128 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8395 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4386 4386 Size: 8395 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2343 3555 7055 1239 Size: 1104 1212 3500 2579 Fragments arranged by size: 3500 2579 1212 1104 Bpu10I CC'TnA_GC Cuts at: 5622 5622 Size: 8395 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2783 3519 4471 6520 7385 7683 2783 Size: 736 952 2049 865 298 3495 Fragments arranged by size: 3495 2049 952 865 736 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3585 7058 882 Size: 617 2086 3473 2219 Fragments arranged by size: 3473 2219 2086 617 BsaAI yAC'GTr Cuts at: 493 1204 3511 5453 6186 493 Size: 711 2307 1942 733 2702 Fragments arranged by size: 2702 2307 1942 733 711 BsaBI GATnn'nnATC Cuts at: 2997 8389 2997 Size: 5392 3003 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2413 6202 6584 7219 Size: 53 83 186 936 880 3789 382 635 Cuts at: 7219 275 Size: 1451 Fragments arranged by size: 3789 1451 936 880 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6828 7226 7500 7647 8058 6828 Size: 398 274 147 411 7165 Fragments arranged by size: 7165 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3115 3145 3942 3972 1178 Size: 30 165 30 1712 30 797 30 5601 Fragments arranged by size: 5601 1712 797 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3522 4168 1186 Size: 2336 646 5413 Fragments arranged by size: 5413 2336 646 BseYI C'CCAG_C Cuts at: 3911 7598 8094 3911 Size: 3687 496 4212 Fragments arranged by size: 4212 3687 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2263 3078 3120 4851 5433 1163 Size: 573 527 815 42 1731 582 4125 Fragments arranged by size: 4125 1731 815 582 573 527 42 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5845 6606 7634 1540 Size: 275 186 3844 761 1028 2301 Fragments arranged by size: 3844 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4095 4258 4367 6456 6541 7612 4095 Size: 163 109 2089 85 1071 4878 Fragments arranged by size: 4878 2089 1071 163 109 85 BsmI GAATG_Cn' Cuts at: 5906 5999 5906 Size: 93 8302 BsmBI CGTCTCn'nnnn_ Cuts at: 3046 3133 4514 3046 Size: 87 1381 6927 Fragments arranged by size: 6927 1381 87 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3409 4095 4258 4367 5714 6456 6541 Size: 399 1619 686 163 109 1347 742 85 Cuts at: 6541 7612 1391 Size: 1071 2174 Fragments arranged by size: 2174 1619 1347 1071 742 686 399 163 109 85 BspEI T'CCGG_A Cuts at: 8058 8058 Size: 8395 BspHI T'CATG_A Cuts at: 6284 8014 6284 Size: 1730 6665 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5750 844 Size: 4906 3489 BsrBI CCG'CTC Cuts at: 1394 1823 6282 1394 Size: 429 4459 3507 Fragments arranged by size: 4459 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2803 2976 3261 4398 6890 7064 65 Size: 1183 1555 173 285 1137 2492 174 1396 Fragments arranged by size: 2492 1555 1396 1183 1137 285 174 173 BsrFI r'CCGG_y Cuts at: 1700 1810 7039 7226 1700 Size: 110 5229 187 2869 Fragments arranged by size: 5229 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3226 5271 5462 96 Size: 3130 2045 191 3029 Fragments arranged by size: 3130 3029 2045 191 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8395 BssSI C'ACGA_G Cuts at: 6455 7467 6455 Size: 1012 7383 BstAPI GCAn_nnn'nTGC Cuts at: 794 2384 2975 3103 4637 794 Size: 1590 591 128 1534 4552 Fragments arranged by size: 4552 1590 1534 591 128 BstBI TT'CG_AA Cuts at: 7199 7199 Size: 8395 BstEII G'GTnAC_C Cuts at: 1184 3915 1184 Size: 2731 5664 BstXI CCAn_nnnn'nTGG Cuts at: 2038 2125 2038 Size: 87 8308 BstYI r'GATC_y Cuts at: 1972 2641 3703 4199 5131 5725 6092 6477 6494 Size: 669 1062 496 932 594 367 385 17 Cuts at: 6494 7946 8032 8044 8390 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1062 932 669 594 496 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2865 2865 Size: 8395 BtgI C'CryG_G Cuts at: 513 1065 2004 4907 513 Size: 552 939 2903 4001 Fragments arranged by size: 4001 2903 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7656 Fragments arranged by size: 7656 683 56 BtsI GCAGTG_nn' Cuts at: 2580 5911 6705 6725 2580 Size: 3331 794 20 4250 Fragments arranged by size: 4250 3331 794 20 ClaI AT'CG_AT Cuts at: 1554 3489 1554 Size: 1935 6460 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8360 DraI TTT'AAA Cuts at: 3154 5218 5699 3154 Size: 2064 481 5850 Fragments arranged by size: 5850 2064 481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7402 809 Size: 507 6086 1802 Fragments arranged by size: 6086 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2602 4137 5842 Size: 54 1425 50 275 18 772 1535 1705 Cuts at: 5842 6731 8360 8 Size: 889 1629 43 Fragments arranged by size: 1705 1629 1535 1425 889 772 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5842 1537 Size: 275 4030 4090 Fragments arranged by size: 4090 4030 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3159 3324 5068 5570 6325 1353 Size: 1806 165 1744 502 755 3423 Fragments arranged by size: 3423 1806 1744 755 502 165 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6982 7356 7502 387 Size: 1064 138 5393 374 146 1280 Fragments arranged by size: 5393 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4093 4256 4093 Size: 163 8232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2343 3272 3555 3636 3824 6458 7055 Size: 393 711 929 283 81 188 2634 597 Cuts at: 7055 7842 8110 1239 Size: 787 268 1524 Fragments arranged by size: 2634 1524 929 787 711 597 393 283 268 188 81 EcoO109I rG'GnC_Cy Cuts at: 3405 3406 3405 Size: 1 8394 EcoRI G'AATT_C Cuts at: 3113 5594 5704 6082 3113 Size: 2481 110 378 5426 Fragments arranged by size: 5426 2481 378 110 EcoRV GAT'ATC Cuts at: 4929 5308 4929 Size: 379 8016 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2324 2522 3641 4939 2324 Size: 198 1119 1298 5780 Fragments arranged by size: 5780 1298 1119 198 FspI TGC'GCA Cuts at: 4334 6901 4334 Size: 2567 5828 HaeII r_GCGC'y Cuts at: 1678 2016 7542 1678 Size: 338 5526 2531 Fragments arranged by size: 5526 2531 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2672 2704 3115 3147 3482 Size: 32 135 32 1100 32 411 32 335 Cuts at: 3482 3514 4191 4223 5292 5324 5648 5680 7036 Size: 32 677 32 1069 32 324 32 1356 Cuts at: 7036 7068 7110 7142 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1356 1100 1069 677 411 335 324 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2556 2691 3378 3882 4709 5739 Size: 988 281 618 135 687 504 827 1030 Cuts at: 5739 5985 669 Size: 246 3079 Fragments arranged by size: 3079 1030 988 827 687 618 504 281 246 135 HindIII A'AGCT_T Cuts at: 748 4593 748 Size: 3845 4550 HpaI GTT'AAC Cuts at: 3378 3882 5985 3378 Size: 504 2103 5788 Fragments arranged by size: 5788 2103 504 KpnI G_GTAC'C Cuts at: 5720 5720 Size: 8395 MfeI C'AATT_G Cuts at: 2262 5994 2262 Size: 3732 4663 MluI A'CGCG_T Cuts at: 8271 8271 Size: 8395 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2106 3023 3568 4548 5409 7509 7693 1305 Size: 801 917 545 980 861 2100 184 2007 Fragments arranged by size: 2100 2007 980 917 861 801 545 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2604 4139 10 Size: 54 1425 343 772 1535 4266 Fragments arranged by size: 4266 1535 1425 772 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2285 4655 5263 6353 6712 6871 518 Size: 648 1119 2370 608 1090 359 159 2042 Fragments arranged by size: 2370 2042 1119 1090 648 608 359 159 MspA1I CmG'CkG Cuts at: 1606 3062 3740 4064 4451 4628 5811 6488 7636 Size: 1456 678 324 387 177 1183 677 1148 Cuts at: 7636 7881 1606 Size: 245 2120 Fragments arranged by size: 2120 1456 1183 1148 678 677 387 324 245 177 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6904 Fragments arranged by size: 6904 939 552 NdeI CA'TA_TG Cuts at: 387 4786 4942 387 Size: 4399 156 3840 Fragments arranged by size: 4399 3840 156 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8395 NotI GC'GGCC_GC Cuts at: 5842 5842 Size: 8395 NsiI A_TGCA'T Cuts at: 4125 4642 4125 Size: 517 7878 NspI r_CATG'y Cuts at: 1132 1229 2290 3376 4127 4339 4434 5463 5753 Size: 97 1061 1086 751 212 95 1029 290 Cuts at: 5753 7298 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1086 1061 1029 751 290 212 97 95 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8395 PciI A'CATG_T Cuts at: 3372 5459 7294 3372 Size: 2087 1835 4473 Fragments arranged by size: 4473 2087 1835 PflMI CCAn_nnn'nTGG Cuts at: 1108 4288 1108 Size: 3180 5215 PmeI GTTT'AAAC Cuts at: 5699 5699 Size: 8395 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3942 3974 6464 6496 7977 8009 3942 Size: 32 2490 32 1481 32 4328 Fragments arranged by size: 4328 2490 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 5289 1613 Size: 3676 4719 PsiI TTA'TAA Cuts at: 2897 2937 5192 5965 2897 Size: 40 2255 773 5327 Fragments arranged by size: 5327 2255 773 40 PspOMI G'GGCC_C Cuts at: 3405 3405 Size: 8395 PspXI vC'TCGA_Gb Cuts at: 4425 4425 Size: 8395 PstI C_TGCA'G Cuts at: 830 2109 5424 5747 830 Size: 1279 3315 323 3478 Fragments arranged by size: 3478 3315 1279 323 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8395 PvuII CAG'CTG Cuts at: 3062 3740 4064 4451 4628 3062 Size: 678 324 387 177 6829 Fragments arranged by size: 6829 678 387 324 177 SacI G_AGCT'C Cuts at: 4095 4258 4095 Size: 163 8232 SalI G'TCGA_C Cuts at: 1936 5737 1936 Size: 3801 4594 SapI GCTCTTCn'nnn_ Cuts at: 3324 3324 Size: 8395 SbfI CC_TGCA'GG Cuts at: 5747 5747 Size: 8395 ScaI AGT'ACT Cuts at: 2825 5418 6643 8355 2825 Size: 2593 1225 1712 2865 Fragments arranged by size: 2865 2593 1712 1225 SexAI A'CCwGG_T Cuts at: 3875 3875 Size: 8395 SfcI C'TryA_G Cuts at: 826 1045 2105 3081 3885 4045 4452 4494 5420 Size: 219 1060 976 804 160 407 42 926 Cuts at: 5420 5743 6878 7559 7750 826 Size: 323 1135 681 191 1471 Fragments arranged by size: 1471 1135 1060 976 926 804 681 407 323 219 191 160 42 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8395 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8395 SmaI CCC'GGG Cuts at: 1872 5722 1872 Size: 3850 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 3534 4425 4486 4555 4913 Size: 197 926 819 772 891 61 69 358 Cuts at: 4913 6499 7400 7662 820 Size: 1586 901 262 1553 Fragments arranged by size: 1586 1553 926 901 891 819 772 358 262 197 69 61 SnaBI TAC'GTA Cuts at: 493 3511 6186 493 Size: 3018 2675 2702 Fragments arranged by size: 3018 2702 2675 SpeI A'CTAG_T Cuts at: 152 4703 5690 152 Size: 4551 987 2857 Fragments arranged by size: 4551 2857 987 SphI G_CATG'C Cuts at: 1132 4339 4434 5753 1132 Size: 3207 95 1319 3774 Fragments arranged by size: 3774 3207 1319 95 SspI AAT'ATT Cuts at: 5 52 2902 6319 5 Size: 47 2850 3417 2081 Fragments arranged by size: 3417 2850 2081 47 StuI AGG'CCT Cuts at: 1517 3940 1517 Size: 2423 5972 StyI C'CwwG_G Cuts at: 513 1065 2004 2466 3802 5082 5834 513 Size: 552 939 462 1336 1280 752 3074 Fragments arranged by size: 3074 1336 1280 939 752 552 462 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 5448 6422 6439 6592 6777 7222 325 Size: 1255 3868 974 17 153 185 445 1498 Fragments arranged by size: 3868 1498 1255 974 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2823 3226 3427 Size: 274 80 33 51 1443 846 403 201 Cuts at: 3427 5271 5416 5462 6641 8353 96 Size: 1844 145 46 1179 1712 138 Fragments arranged by size: 1844 1712 1443 1179 846 403 274 201 145 138 80 51 46 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 8355 8387 8355 Size: 32 8363 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 8395 XbaI T'CTAG_A Cuts at: 4084 5281 5731 4084 Size: 1197 450 6748 Fragments arranged by size: 6748 1197 450 XhoI C'TCGA_G Cuts at: 1943 4425 1943 Size: 2482 5913 XmaI C'CCGG_G Cuts at: 1870 5720 1870 Size: 3850 4545 XmnI GAAnn'nnTTC Cuts at: 2307 3105 3621 3957 6524 2307 Size: 798 516 336 2567 4178 Fragments arranged by size: 4178 2567 798 516 336 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2414 6203 276 Size: 53 83 186 936 880 3789 2468 Fragments arranged by size: 3789 2468 936 880 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI XcmI