Restriction map for
pFN21AA0799
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# NO CUT ENZYMES ( 46 enzymes) #
##################################
Acc65I AclI AscI AseI AsnI
Asp718I AviII AvrII BbrPI BbvCI
BlnI BsaI BsiWI BspDI BssHII
BstEII BstPI ClaI DraI EagI
EclXI Eco52I EcoO65I FseI FspI
KpnI KspI NdeI NheI NotI
NruI PacI PmaCI PmeI PmlI
PshBI Psp1406I SacII SbfI SexAI
SfiI SgrAI SpeI Sse8387I SwaI
XbaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 2092 GACGTC GACGT^C
AflII 5026 CTTAAG C^TTAAG
AgeI 3401 ACCGGT A^CCGGT
Alw44I 2106 GTGCAC G^TGCAC
ApaI 2249 GGGCCC GGGCC^C
ApaLI 2106 GTGCAC G^TGCAC
AspI 5932 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 5686 GGATCC G^GATCC
BbeI 368 GGCGCC GGCGC^C
BfrI 5026 CTTAAG C^TTAAG
BglII 1069 AGATCT A^GATCT
BlpI 1875 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1875 GCTAAGC GC^T[ACGT]AGC
BsaAI 6024 TACGTA [CT]AC^GT[GA]
BsiEI 4502 CGATCG CG[GA][CT]^CG
BsmBI 5937 CGTCTC CGTCTC(1/5)
BspHI 6095 TCATGA T^CATGA
BssSI 2109 CACGAG CACGAG(-5/-1)
Bst1107I 995 GTATAC GTA^TAC
BstBI 2989 TTCGAA TT^CGAA
BstZ17I 995 GTATAC GTA^TAC
CelII 1875 GCTAAGC GC^T[ACGT]AGC
CpoI 2021 CGGTCCG CG^G[AT]CCG
DraIII 4455 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2851 GACGAGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2361 CCTGAAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 4174 GAATTC G^AATTC
EcoRV 4534 GATATC GAT^ATC
EcoT22I 3955 ATGCAT ATGCA^T
HindIII 614 AAGCTT A^AGCTT
HpaI 1621 GTTAAC GTT^AAC
KasI 368 GGCGCC G^GCGCC
MfeI 2428 CAATTG C^AATTG
MluI 3926 ACGCGT A^CGCGT
MunI 2428 CAATTG C^AATTG
NaeI 365 GCCGGC GCC^GGC
NarI 368 GGCGCC GG^CGCC
NgoMIV 365 GCCGGC G^CCGGC
NsiI 3955 ATGCAT ATGCA^T
NspV 2989 TTCGAA TT^CGAA
PflFI 5932 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3401 ACCGGT A^CCGGT
PvuI 4502 CGATCG CGAT^CG
RcaI 6095 TCATGA T^CATGA
RsrII 2021 CGGTCCG CG^G[AT]CCG
SalI 5251 GTCGAC G^TCGAC
SapI 2598 GAAGAGC GCTCTTC(1/4)
SfoI 368 GGCGCC GGC^GCC
SfuI 2989 TTCGAA TT^CGAA
SmaI 763 CCCGGG CCC^GGG
SnaBI 6024 TACGTA TAC^GTA
SspI 700 AATATT AAT^ATT
StuI 5193 AGGCCT AGG^CCT
Tth111I 5932 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 763 CCCGGG C^CCGGG
XmaI 763 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AhdI 199 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1276 GACTTCAAGTC
Aor51HI 3707 AGCGCT AGC^GCT
6128 AGCGCT
Asp700I 4365 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4972 GAACAGTTTC
AspEI 199 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1276 GACTTCAAGTC
BcgI 2098 CGAGGGAAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2111 CGAGCCTGCTGC
BciVI 1791 GGATAC GTATCC(6/5)
4964 GGATAC
BclI 4061 TGATCA T^GATCA
5837 TGATCA
BglI 371 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
5117 GCCATGGCGGC
BsaBI 3834 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5139 GATCACCATC
Eam1105I 199 GACATGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1276 GACTTCAAGTC
Eco47III 3707 AGCGCT AGC^GCT
6128 AGCGCT
FbaI 4061 TGATCA T^GATCA
5837 TGATCA
MamI 3834 GATCGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
5139 GATCACCATC
PaeR7I 2824 CTCGAG C^TCGAG
5759 CTCGAG
ScaI 64 AGTACT AGT^ACT
1379 AGTACT
XhoI 2824 CTCGAG C^TCGAG
5759 CTCGAG
XmnI 4365 GAAGATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4972 GAACAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AccII AccIII AciI AcsI
AcyI AfaI AflIII AluI AlwI
AlwNI AocI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
Bsp1407I BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MroI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PshAI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII