(Circular) (Six-base) MAPSORT of: pFN21AA0799.seq Check: 7039 from: 1 to: 10879 pFN21AA0799 10879 bp With 182 enzymes: SgfI * January 16, 2009 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4651 4651 Size: 10879 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4101 8689 278 Size: 53 83 186 936 2565 4588 2468 Fragments arranged by size: 4588 2565 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3001 3189 7257 7950 8222 1937 Size: 1064 188 4068 693 272 4594 Fragments arranged by size: 4594 4068 1064 693 272 188 Acc65I G'GTAC_C Cuts at: 8200 8200 Size: 10879 AclI AA'CG_TT Cuts at: 1563 9006 9379 1563 Size: 7443 373 3063 Fragments arranged by size: 7443 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3362 4939 5076 6266 6683 8942 10326 10594 Size: 1730 1577 137 1190 417 2259 1384 268 Cuts at: 10594 1632 Size: 1917 Fragments arranged by size: 2259 1917 1730 1577 1384 1190 417 268 137 AfeI AGC'GCT Cuts at: 5714 8135 5714 Size: 2421 8458 AflII C'TTAA_G Cuts at: 820 1017 7031 820 Size: 197 6014 4668 Fragments arranged by size: 6014 4668 197 AflIII A'CryG_T Cuts at: 1203 3772 5931 7224 7734 9778 10755 1203 Size: 2569 2159 1293 510 2044 977 1327 Fragments arranged by size: 2569 2159 2044 1327 1293 977 510 AgeI A'CCGG_T Cuts at: 5406 9710 5406 Size: 4304 6575 AhdI GACnn_n'nnGTC Cuts at: 2209 3286 9608 2209 Size: 1077 6322 3480 Fragments arranged by size: 6322 3480 1077 AleI CACnn'nnGTG Cuts at: 1166 5123 6164 1166 Size: 3957 1041 5881 Fragments arranged by size: 5881 3957 1041 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4136 5174 6938 1184 Size: 2952 1038 1764 5125 Fragments arranged by size: 5125 2952 1764 1038 AlwNI CAG_nnn'CTG Cuts at: 4204 4729 4759 6345 6529 6739 6898 7516 7729 Size: 525 30 1586 184 210 159 618 213 Cuts at: 7729 8622 10194 4204 Size: 893 1572 4889 Fragments arranged by size: 4889 1586 1572 893 618 525 213 210 184 159 30 ApaI G_GGCC'C Cuts at: 4258 4258 Size: 10879 ApaLI G'TGCA_C Cuts at: 4111 8936 10092 4111 Size: 4825 1156 4898 Fragments arranged by size: 4898 4825 1156 ApoI r'AATT_y Cuts at: 1493 2077 2834 3023 4901 6179 7352 7793 8188 Size: 584 757 189 1878 1278 1173 441 395 Cuts at: 8188 8414 8566 10675 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1878 1697 1278 1173 757 584 441 395 226 189 152 AseI AT'TA_AT Cuts at: 160 1032 9433 160 Size: 872 8401 1606 Fragments arranged by size: 8401 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10879 AvaI C'yCGr_G Cuts at: 1870 1943 2768 4829 5395 7065 7764 8204 1870 Size: 73 825 2061 566 1670 699 440 4545 Fragments arranged by size: 4545 2061 1670 825 699 566 440 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 7496 7529 7496 Size: 33 10846 BamHI G'GATC_C Cuts at: 7691 8209 8576 7691 Size: 518 367 9994 Fragments arranged by size: 9994 518 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2373 5125 5300 5399 5780 Size: 325 217 627 586 2752 175 99 381 Cuts at: 5780 5996 8014 8200 9655 618 Size: 216 2018 186 1455 1842 Fragments arranged by size: 2752 2018 1842 1455 627 586 381 325 217 216 186 175 99 BanII G_rGCy'C Cuts at: 1391 2226 4258 4548 4785 5681 8198 1391 Size: 835 2032 290 237 896 2517 4072 Fragments arranged by size: 4072 2517 2032 896 835 290 237 BbeI G_GCGC'C Cuts at: 2377 2377 Size: 10879 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2095 2576 2642 2898 4039 4172 6225 Size: 971 196 481 66 256 1141 133 2053 Cuts at: 6225 7975 928 Size: 1750 3832 Fragments arranged by size: 3832 2053 1750 1141 971 481 256 196 133 66 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4092 4105 4126 4139 9070 9104 1903 Size: 34 2155 13 21 13 4931 34 3678 Fragments arranged by size: 4931 3678 2155 34 34 21 13 13 BciVI GTATCCnnnnn_n' Cuts at: 3790 6963 8771 9981 3790 Size: 3173 1808 1210 4688 Fragments arranged by size: 4688 3173 1808 1210 BclI T'GATC_A Cuts at: 1548 6066 7842 1548 Size: 4518 1776 4585 Fragments arranged by size: 4585 4518 1776 BfrBI ATG'CAT Cuts at: 5962 5962 Size: 10879 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2382 7128 9490 Size: 107 122 71 631 605 710 4746 2362 Cuts at: 9490 136 Size: 1525 Fragments arranged by size: 4746 2362 1525 710 631 605 122 107 71 BglII A'GATC_T Cuts at: 3074 10874 3074 Size: 7800 3079 BlpI GC'TnA_GC Cuts at: 3881 8296 3881 Size: 4415 6464 Bme1580I G_kGCm'C Cuts at: 1790 3046 4115 4258 5130 5783 5999 6858 8017 Size: 1256 1069 143 872 653 216 859 1159 Cuts at: 8017 8940 10096 1790 Size: 923 1156 2573 Fragments arranged by size: 2573 1256 1159 1156 1069 923 872 859 653 216 143 BmgBI CAC'GTC Cuts at: 1318 2947 7255 1318 Size: 1629 4308 4942 Fragments arranged by size: 4942 4308 1629 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 5133 6329 8610 Size: 416 53 83 399 24 3716 1196 2281 Cuts at: 8610 9568 442 Size: 958 1753 Fragments arranged by size: 3716 2281 1753 1196 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10879 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6956 6956 Size: 10879 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3675 4013 4226 4658 4703 4814 5188 6761 Size: 2436 338 213 432 45 111 374 1573 Cuts at: 6761 7469 7493 7655 8158 9539 1239 Size: 708 24 162 503 1381 2579 Fragments arranged by size: 2579 2436 1573 1381 708 503 432 374 338 213 162 111 45 24 Bpu10I CC'TnA_GC Cuts at: 6908 6908 Size: 10879 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2501 3528 5585 5630 5759 6926 6935 7568 9004 Size: 1027 2057 45 129 1167 9 633 1436 Cuts at: 9004 9869 10167 2501 Size: 865 298 3213 Fragments arranged by size: 3213 2057 1436 1167 1027 865 633 298 129 45 9 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 9542 882 Size: 617 8043 2219 Fragments arranged by size: 8043 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 8031 8670 493 Size: 711 6827 639 2702 Fragments arranged by size: 6827 2702 711 639 BsaBI GATnn'nnATC Cuts at: 5843 7148 10873 5843 Size: 1305 3725 5849 Fragments arranged by size: 5849 3725 1305 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2210 2374 4098 5010 Size: 53 83 186 936 677 164 1724 912 Cuts at: 5010 7938 8686 9068 9703 275 Size: 2928 748 382 635 1451 Fragments arranged by size: 2928 1724 1451 936 912 748 677 635 382 186 164 83 53 BsaWI w'CCGG_w Cuts at: 2106 4866 5073 5406 7683 9312 9710 9984 10131 Size: 2760 207 333 2277 1629 398 274 147 Cuts at: 10131 10542 2106 Size: 411 2443 Fragments arranged by size: 2760 2443 2277 1629 411 398 333 274 207 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2279 2309 3492 3522 4790 Size: 30 165 30 876 30 1183 30 1268 Cuts at: 4790 4820 5186 5216 5237 5267 6734 6764 7928 Size: 30 366 30 21 30 1467 30 1164 Cuts at: 7928 7958 1178 Size: 30 4099 Fragments arranged by size: 4099 1467 1268 1183 1164 876 366 165 30 30 30 30 30 30 30 30 30 21 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2204 3877 4363 4366 4811 4949 4988 4991 Size: 1018 1673 486 3 445 138 39 3 Cuts at: 4991 5111 5191 5671 5780 6128 7730 7733 7747 Size: 120 80 480 109 348 1602 3 14 Cuts at: 7747 1186 Size: 4318 Fragments arranged by size: 4318 1673 1602 1018 486 480 445 348 138 120 109 80 39 14 3 3 3 BseYI C'CCAG_C Cuts at: 4016 4159 4554 5033 5240 6522 7169 10082 10578 Size: 143 395 479 207 1282 647 2913 496 Cuts at: 10578 4016 Size: 4317 Fragments arranged by size: 4317 2913 1282 647 496 479 395 207 143 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2106 4828 5195 6074 6898 7520 1163 Size: 573 370 2722 367 879 824 622 4522 Fragments arranged by size: 4522 2722 879 824 622 573 370 367 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6510 8329 9090 10118 1540 Size: 275 186 4509 1819 761 1028 2301 Fragments arranged by size: 4509 2301 1819 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2147 2226 4115 4548 4785 5043 8940 9025 10096 Size: 79 1889 433 237 258 3897 85 1071 Cuts at: 10096 2147 Size: 2930 Fragments arranged by size: 3897 2930 1889 1071 433 258 237 85 79 BsmI GAATG_Cn' Cuts at: 3482 3862 4337 5992 8390 8483 3482 Size: 380 475 1655 2398 93 5878 Fragments arranged by size: 5878 2398 1655 475 380 93 BsmBI CGTCTCn'nnnn_ Cuts at: 7948 7948 Size: 10879 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2147 2226 3046 4115 4258 4548 4785 Size: 399 357 79 820 1069 143 290 237 Cuts at: 4785 5043 5130 5681 5783 5999 6858 8017 8198 Size: 258 87 551 102 216 859 1159 181 Cuts at: 8198 8940 9025 10096 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1159 1071 1069 859 820 742 551 399 357 290 258 237 216 181 143 102 87 85 79 BspEI T'CCGG_A Cuts at: 2106 4866 5073 7683 10542 2106 Size: 2760 207 2610 2859 2443 Fragments arranged by size: 2859 2760 2610 2443 207 BspHI T'CATG_A Cuts at: 8100 8768 10498 8100 Size: 668 1730 8481 Fragments arranged by size: 8481 1730 668 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2477 4651 4710 6807 7350 8234 844 Size: 1633 2174 59 2097 543 884 3489 Fragments arranged by size: 3489 2174 2097 1633 884 543 59 BsrBI CCG'CTC Cuts at: 1394 1823 4768 4879 5628 6634 8766 1394 Size: 429 2945 111 749 1006 2132 3507 Fragments arranged by size: 3507 2945 2132 1006 749 429 111 BsrDI GCAATG_nn' Cuts at: 65 1248 2635 2946 3280 9374 9548 65 Size: 1183 1387 311 334 6094 174 1396 Fragments arranged by size: 6094 1396 1387 1183 334 311 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2370 5406 5496 6429 9523 9710 1700 Size: 110 560 3036 90 933 3094 187 2869 Fragments arranged by size: 3094 3036 2869 933 560 187 110 90 BsrGI T'GTAC_A Cuts at: 96 2643 5511 5550 6882 96 Size: 2547 2868 39 1332 4093 Fragments arranged by size: 4093 2868 2547 1332 39 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10879 BssSI C'ACGA_G Cuts at: 4114 8939 9951 4114 Size: 4825 1012 5042 Fragments arranged by size: 5042 4825 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3934 4759 6799 794 Size: 3140 825 2040 4874 Fragments arranged by size: 4874 3140 2040 825 BstBI TT'CG_AA Cuts at: 4995 9683 4995 Size: 4688 6191 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10879 BstXI CCAn_nnnn'nTGG Cuts at: 4915 5240 7834 8080 4915 Size: 325 2594 246 7714 Fragments arranged by size: 7714 2594 325 246 BstYI r'GATC_y Cuts at: 1972 3074 3156 4671 5949 6894 7691 8209 8576 Size: 1102 82 1515 1278 945 797 518 367 Cuts at: 8576 8961 8978 10430 10516 10528 10874 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1515 1452 1278 1102 945 797 518 385 367 346 86 82 17 12 BstZ17I GTA'TAC Cuts at: 3002 3002 Size: 10879 Bsu36I CC'TnA_GG Cuts at: 3143 4092 7670 7908 3143 Size: 949 3578 238 6114 Fragments arranged by size: 6114 3578 949 238 BtgI C'CryG_G Cuts at: 513 1065 2004 2227 2355 3210 4242 5937 7110 Size: 552 939 223 128 855 1032 1695 1173 Cuts at: 7110 7123 7206 7346 7453 7695 7821 513 Size: 13 83 140 107 242 126 3571 Fragments arranged by size: 3571 1695 1173 1032 939 855 552 242 223 140 128 126 107 83 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2314 2395 5097 487 Size: 683 56 1088 81 2702 6269 Fragments arranged by size: 6269 2702 1088 683 81 56 BtsI GCAGTG_nn' Cuts at: 2798 3030 3857 6091 6874 8395 9189 9209 2798 Size: 232 827 2234 783 1521 794 20 4468 Fragments arranged by size: 4468 2234 1521 827 794 783 232 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10879 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 7879 7914 529 Size: 35 7315 35 3494 Fragments arranged by size: 7315 3494 35 35 DraI TTT'AAA Cuts at: 8183 8183 Size: 10879 DraIII CAC_nnn'GTG Cuts at: 6465 6465 Size: 10879 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4862 9886 809 Size: 507 3546 5024 1802 Fragments arranged by size: 5024 3546 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 3207 4239 Size: 54 1425 50 275 18 223 1154 1032 Cuts at: 4239 5044 5853 6075 7203 7249 7456 7824 8091 Size: 805 809 222 1128 46 207 368 267 Cuts at: 8091 8326 9215 10844 8 Size: 235 889 1629 43 Fragments arranged by size: 1629 1425 1154 1128 1032 889 809 805 368 275 267 235 223 222 207 54 50 46 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8326 1537 Size: 275 6514 4090 Fragments arranged by size: 6514 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3147 4215 4473 4528 4598 4662 5095 5445 Size: 1794 1068 258 55 70 64 433 350 Cuts at: 5445 7019 7554 7634 8809 1353 Size: 1574 535 80 1175 3423 Fragments arranged by size: 3423 1794 1574 1175 1068 535 433 350 258 80 70 64 55 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5717 7660 7690 8143 9466 9840 Size: 1064 138 4128 1943 30 453 1323 374 Cuts at: 9840 9986 387 Size: 146 1280 Fragments arranged by size: 4128 1943 1323 1280 1064 453 374 146 138 30 EcoICRI GAG'CTC Cuts at: 2224 4546 4783 2224 Size: 2322 237 8320 Fragments arranged by size: 8320 2322 237 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3362 3675 4013 4226 4658 4703 4814 Size: 393 1730 313 338 213 432 45 111 Cuts at: 4814 4939 5076 5188 6266 6683 6761 7469 7493 Size: 125 137 112 1078 417 78 708 24 Cuts at: 7493 7655 8158 8942 9539 10326 10594 1239 Size: 162 503 784 597 787 268 1524 Fragments arranged by size: 1730 1524 1078 787 784 708 597 503 432 417 393 338 313 268 213 162 137 125 112 111 78 45 24 EcoNI CCTnn'n_nnAGG Cuts at: 4370 4370 Size: 10879 EcoO109I rG'GnC_Cy Cuts at: 4297 4699 5101 5382 5641 6324 6502 7339 7665 Size: 402 402 281 259 683 178 837 326 Cuts at: 7665 4297 Size: 7511 Fragments arranged by size: 7511 837 683 402 402 326 281 259 178 EcoRI G'AATT_C Cuts at: 6179 8188 8566 6179 Size: 2009 378 8492 Fragments arranged by size: 8492 2009 378 EcoRV GAT'ATC Cuts at: 6541 6541 Size: 10879 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4963 4963 Size: 10879 FspI TGC'GCA Cuts at: 9385 9385 Size: 10879 HaeII r_GCGC'y Cuts at: 1678 2377 2446 5716 8137 10026 1678 Size: 699 69 3270 2421 1889 2531 Fragments arranged by size: 3270 2531 2421 1889 699 69 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2558 2590 2900 Size: 32 135 32 623 32 331 32 310 Cuts at: 2900 2932 3141 3173 3272 3304 4063 4095 4663 Size: 32 209 32 99 32 759 32 568 Cuts at: 4663 4695 5237 5269 6734 6766 7683 7715 7758 Size: 32 542 32 1465 32 917 32 43 Cuts at: 7758 7790 7928 7960 9520 9552 9594 9626 1373 Size: 32 138 32 1560 32 42 32 2626 Fragments arranged by size: 2626 1560 1465 917 759 623 568 542 331 310 209 138 135 99 43 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2540 3628 7258 7605 8223 8469 Size: 988 281 602 1088 3630 347 618 246 Cuts at: 8469 669 Size: 3079 Fragments arranged by size: 3630 3079 1088 988 618 602 347 281 246 HindIII A'AGCT_T Cuts at: 748 2619 748 Size: 1871 9008 HpaI GTT'AAC Cuts at: 3628 8469 3628 Size: 4841 6038 KasI G'GCGC_C Cuts at: 2373 2373 Size: 10879 KpnI G_GTAC'C Cuts at: 8204 8204 Size: 10879 MfeI C'AATT_G Cuts at: 4433 8478 4433 Size: 4045 6834 MluI A'CGCG_T Cuts at: 5931 10755 5931 Size: 4824 6055 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4167 5358 9993 10177 1305 Size: 2862 1191 4635 184 2007 Fragments arranged by size: 4635 2862 2007 1191 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2055 3209 7205 7458 7826 Size: 54 1425 343 223 1154 3996 253 368 Cuts at: 7826 8093 10 Size: 267 2796 Fragments arranged by size: 3996 2796 1425 1154 368 343 267 253 223 54 MslI CAynn'nnrTG Cuts at: 518 1166 3603 5123 5849 6164 8117 8123 8837 Size: 648 2437 1520 726 315 1953 6 714 Cuts at: 8837 9196 9355 518 Size: 359 159 2042 Fragments arranged by size: 2437 2042 1953 1520 726 714 648 359 315 159 6 MspA1I CmG'CkG Cuts at: 1606 2260 2430 2683 3973 4159 5208 5370 5517 Size: 654 170 253 1290 186 1049 162 147 Cuts at: 5517 5589 6529 6888 7169 8295 8972 10120 10365 Size: 72 940 359 281 1126 677 1148 245 Cuts at: 10365 1606 Size: 2120 Fragments arranged by size: 2120 1290 1148 1126 1049 940 677 654 359 281 253 245 186 170 162 147 72 NaeI GCC'GGC Cuts at: 2372 2372 Size: 10879 NarI GG'CG_CC Cuts at: 2374 2374 Size: 10879 NcoI C'CATG_G Cuts at: 513 1065 2004 2227 2355 3210 4242 7123 7206 Size: 552 939 223 128 855 1032 2881 83 Cuts at: 7206 7346 7453 7821 513 Size: 140 107 368 3571 Fragments arranged by size: 3571 2881 1032 939 855 552 368 223 140 128 107 83 NdeI CA'TA_TG Cuts at: 387 387 Size: 10879 NgoMIV G'CCGG_C Cuts at: 2370 2370 Size: 10879 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10879 NotI GC'GGCC_GC Cuts at: 8326 8326 Size: 10879 NsiI A_TGCA'T Cuts at: 5964 5964 Size: 10879 NspI r_CATG'y Cuts at: 1132 1229 3590 3776 3928 4932 5230 6906 7228 Size: 97 2361 186 152 1004 298 1676 322 Cuts at: 7228 7738 8237 9782 1132 Size: 510 499 1545 2229 Fragments arranged by size: 2361 2229 1676 1545 1004 510 499 322 298 186 152 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10879 PciI A'CATG_T Cuts at: 3772 7224 7734 9778 3772 Size: 3452 510 2044 4873 Fragments arranged by size: 4873 3452 2044 510 PflMI CCAn_nnn'nTGG Cuts at: 1108 4264 5904 6465 1108 Size: 3156 1640 561 5522 Fragments arranged by size: 5522 3156 1640 561 PfoI T'CCnGG_A Cuts at: 5183 5693 5952 6985 7377 5183 Size: 510 259 1033 392 8685 Fragments arranged by size: 8685 1033 510 392 259 PmeI GTTT'AAAC Cuts at: 8183 8183 Size: 10879 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7986 8018 8948 8980 10461 10493 7986 Size: 32 930 32 1481 32 8372 Fragments arranged by size: 8372 1481 930 32 32 32 PpuMI rG'GwC_Cy Cuts at: 4297 4699 5101 5382 6324 7339 7665 4297 Size: 402 402 281 942 1015 326 7511 Fragments arranged by size: 7511 1015 942 402 402 326 281 PshAI GACnn'nnGTC Cuts at: 1613 2118 4172 6329 1613 Size: 505 2054 2157 6163 Fragments arranged by size: 6163 2157 2054 505 PsiI TTA'TAA Cuts at: 8449 8449 Size: 10879 PspOMI G'GGCC_C Cuts at: 4254 4254 Size: 10879 PspXI vC'TCGA_Gb Cuts at: 4829 7764 4829 Size: 2935 7944 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 6938 6970 6938 Size: 32 10847 PstI C_TGCA'G Cuts at: 830 4128 4707 4755 4764 6144 6735 7521 7578 Size: 3298 579 48 9 1380 591 786 57 Cuts at: 7578 8231 830 Size: 653 3478 Fragments arranged by size: 3478 3298 1380 786 653 591 579 57 48 9 PvuI CG_AT'CG Cuts at: 2001 6510 2001 Size: 4509 6370 PvuII CAG'CTG Cuts at: 2683 3973 4159 5517 6529 6888 2683 Size: 1290 186 1358 1012 359 6674 Fragments arranged by size: 6674 1358 1290 1012 359 186 RsrII CG'GwC_CG Cuts at: 4027 4027 Size: 10879 SacI G_AGCT'C Cuts at: 2226 4548 4785 2226 Size: 2322 237 8320 Fragments arranged by size: 8320 2322 237 SalI G'TCGA_C Cuts at: 1936 7256 8221 1936 Size: 5320 965 4594 Fragments arranged by size: 5320 4594 965 SanDI GG'GwC_CC Cuts at: 6324 7665 6324 Size: 1341 9538 SapI GCTCTTCn'nnn_ Cuts at: 4598 4598 Size: 10879 SbfI CC_TGCA'GG Cuts at: 8231 8231 Size: 10879 ScaI AGT'ACT Cuts at: 2071 3386 9127 10839 2071 Size: 1315 5741 1712 2111 Fragments arranged by size: 5741 2111 1712 1315 SfcI C'TryA_G Cuts at: 826 1045 3831 4124 4703 4751 4760 6140 6731 Size: 219 2786 293 579 48 9 1380 591 Cuts at: 6731 6782 7517 7574 8137 8227 9362 10043 10234 Size: 51 735 57 563 90 1135 681 191 Cuts at: 10234 826 Size: 1471 Fragments arranged by size: 2786 1471 1380 1135 735 681 591 579 563 293 219 191 90 57 51 48 9 SfoI GGC'GCC Cuts at: 2375 2375 Size: 10879 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10879 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 10879 SmaI CCC'GGG Cuts at: 1872 2770 8206 1872 Size: 898 5436 4545 Fragments arranged by size: 5436 4545 898 SmlI C'TyrA_G Cuts at: 820 1017 1943 2516 3543 4829 5600 5645 5774 Size: 197 926 573 1027 1286 771 45 129 Cuts at: 5774 6941 6950 7031 7583 7764 8983 9884 10146 Size: 1167 9 81 552 181 1219 901 262 Cuts at: 10146 820 Size: 1553 Fragments arranged by size: 1553 1286 1219 1167 1027 926 901 771 573 552 262 197 181 129 81 45 9 SnaBI TAC'GTA Cuts at: 493 8031 8670 493 Size: 7538 639 2702 Fragments arranged by size: 7538 2702 639 SpeI A'CTAG_T Cuts at: 152 152 Size: 10879 SphI G_CATG'C Cuts at: 1132 3590 4932 5230 8237 1132 Size: 2458 1342 298 3007 3774 Fragments arranged by size: 3774 3007 2458 1342 298 SspI AAT'ATT Cuts at: 5 52 2707 8803 5 Size: 47 2655 6096 2081 Fragments arranged by size: 6096 2655 2081 47 StuI AGG'CCT Cuts at: 1517 7200 1517 Size: 5683 5196 StyI C'CwwG_G Cuts at: 513 1065 2004 2227 2355 3210 4170 4191 4242 Size: 552 939 223 128 855 960 21 51 Cuts at: 4242 5826 6000 6498 7123 7206 7346 7453 7821 Size: 1584 174 498 625 83 140 107 368 Cuts at: 7821 8318 513 Size: 497 3074 Fragments arranged by size: 3074 1584 960 939 855 625 552 498 497 368 223 174 140 128 107 83 51 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4754 6284 6536 6626 7913 8906 8923 Size: 1255 3174 1530 252 90 1287 993 17 Cuts at: 8923 9076 9261 9706 325 Size: 153 185 445 1498 Fragments arranged by size: 3174 1530 1498 1287 1255 993 445 252 185 153 90 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2069 2364 2643 Size: 274 80 33 51 1443 92 295 279 Cuts at: 2643 3384 5511 5550 5745 6262 6822 6882 7830 Size: 741 2127 39 195 517 560 60 948 Cuts at: 7830 9125 10837 96 Size: 1295 1712 138 Fragments arranged by size: 2127 1712 1443 1295 948 741 560 517 295 279 274 195 138 92 80 60 51 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4790 4822 5186 5218 10839 10871 4790 Size: 32 364 32 5621 32 4798 Fragments arranged by size: 5621 4798 364 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 7940 1316 Size: 6624 4255 XbaI T'CTAG_A Cuts at: 8215 8215 Size: 10879 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5063 7166 8080 5063 Size: 2103 914 7862 Fragments arranged by size: 7862 2103 914 XhoI C'TCGA_G Cuts at: 1943 4829 7764 1943 Size: 2886 2935 5058 Fragments arranged by size: 5058 2935 2886 XmaI C'CCGG_G Cuts at: 1870 2768 8204 1870 Size: 898 5436 4545 Fragments arranged by size: 5436 4545 898 XmnI GAAnn'nnTTC Cuts at: 6374 6981 9008 6374 Size: 607 2027 8245 Fragments arranged by size: 8245 2027 607 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4099 8687 276 Size: 53 83 186 936 2565 4588 2468 Fragments arranged by size: 4588 2565 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI BsiWI FseI FspAI NruI PacI PmlI SacII SexAI SfiI SrfI SwaI