Restriction map for
pFN21AA0791
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbrPI BbvCI BciVI
BcnI BfrI BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiWI BspEI BssHII BstUI BstXI
Bsu36I CelII CfoI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI FauI FseI
FspI HaeII HhaI Hin1I HinP1I
HpaI KasI KpnI KspI MamI
MluI MroI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI PacI PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
RsrII SacII SapI SbfI ScaI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I Van91I
XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AseI 2331 ATTAAT AT^TAAT
AsnI 2331 ATTAAT AT^TAAT
BalI 3023 TGGCCA TGG^CCA
BcgI 2169 GCAGTTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 538 GCCTACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2295 AGATCT A^GATCT
BsiEI 825 CGATCG CG[GA][CT]^CG
BsmBI 1691 CGTCTC CGTCTC(1/5)
BspDI 823 ATCGAT AT^CGAT
BsrBI 2786 CCGCTC CCGCTC(-3/-3)
BsrFI 3448 GCCGGT [GA]^CCGG[CT]
BssSI 3107 CTCGTG CACGAG(-5/-1)
BstAPI 2329 GCATTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 2177 TTCGAA TT^CGAA
BstEII 3898 GGTTACC G^GT[ACGT]ACC
BstPI 3898 GGTTACC G^GT[ACGT]ACC
Cfr10I 3448 GCCGGT [GA]^CCGG[CT]
ClaI 823 ATCGAT AT^CGAT
DraII 27 AGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 1025 CCGTGG C^C[GA][CT]GG
EaeI 3023 TGGCCA [CT]^GGCC[GA]
EcoNI 19 CCTTTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 27 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 3898 GGTTACC G^GT[ACGT]ACC
HapII 3449 CCGG C^CGG
HpaII 3449 CCGG C^CGG
MaeII 637 ACGT A^CGT
MluNI 3023 TGGCCA TGG^CCA
MscI 3023 TGGCCA TGG^CCA
MspI 3449 CCGG C^CGG
NdeI 118 CATATG CA^TATG
NspV 2177 TTCGAA TT^CGAA
PaeR7I 3179 CTCGAG C^TCGAG
PpuMI 27 AGGACCT [GA]G^G[AT]CC[CT]
PshBI 2331 ATTAAT AT^TAAT
PvuI 825 CGATCG CGAT^CG
SalI 2959 GTCGAC G^TCGAC
SfuI 2177 TTCGAA TT^CGAA
SphI 3546 GCATGC GCATG^C
StuI 2711 AGGCCT AGG^CCT
XcmI 1167 CCAGATGACAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3179 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1301 ACATGT A^C[GA][CT]GT
3804 ACATGT
Asp700I 3238 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3985 GAACGGCTTC
AvaI 1532 CTCGGG C^[CT]CG[GA]G
3179 CTCGAG
BamHI 3278 GGATCC G^GATCC
3753 GGATCC
BbsI 1453 GAAGAC GAAGAC(2/6)
2692 GAAGAC
BclI 3033 TGATCA T^GATCA
3732 TGATCA
BpuAI 1453 GAAGAC GAAGAC(2/6)
2692 GAAGAC
BsaI 218 GAGACC GGTCTC(1/5)
1619 GGTCTC
BsoBI 1532 CTCGGG C^[CT]CG[GA]G
3179 CTCGAG
BspHI 2124 TCATGA T^CATGA
3246 TCATGA
BspLU11I 1301 ACATGT A^CATGT
3804 ACATGT
BspMI 913 GCAGGT ACCTGC(4/8)
3642 GCAGGT
Bst1107I 1700 GTATAC GTA^TAC
3800 GTATAC
BstZ17I 1700 GTATAC GTA^TAC
3800 GTATAC
EcoRV 2404 GATATC GAT^ATC
3490 GATATC
EcoT14I 2912 CCAAGG C^C[AT][AT]GG
3573 CCTTGG
FbaI 3033 TGATCA T^GATCA
3732 TGATCA
HgaI 978 GACGC GACGC(5/10)
2070 GCGTC
HindIII 1863 AAGCTT A^AGCTT
3382 AAGCTT
MslI 910 CATGCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1761 CACAAATATG
PleI 353 GACTC GAGTC(4/5)
1945 GAGTC
PstI 2110 CTGCAG CTGCA^G
2630 CTGCAG
RcaI 2124 TCATGA T^CATGA
3246 TCATGA
SexAI 30 ACCTGGT A^CC[AT]GGT
2817 ACCTGGT
SspI 688 AATATT AAT^ATT
1881 AATATT
StyI 2912 CCAAGG C^C[AT][AT]GG
3573 CCTTGG
XbaI 682 TCTAGA T^CTAGA
1983 TCTAGA
XmnI 3238 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3985 GAACGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
Bsp1407I BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
EcoT22I Fnu4HI FokI HaeIII HincII
HindII HinfI HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MfeI
MflI MnlI MseI MspA1I MunI
MvaI MwoI NdeII NlaIII NlaIV
NsiI NspI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII